Short Communication: Characterization of DRB3 Alleles in the MHC of Japanese Shorthorn Cattle by Polymerase Chain Reaction-Sequence-Based Typing

Rhodococcus equi is an uncommon cause of systemic pyogranulomatous infections in goats with macroscopic similarities to caseous lymphadenitis caused by Corynebacterium pseudotuberculosis. Caprine cases have previously been reported to be caused by avirulent R. equi strains. Six cases of R. equi infection in goats yielding 8 R. equi isolates were identified from 2000 to 2017. Lesions varied from bronchopneumonia, vertebral and humeral osteomyelitis, and subcutaneous abscesses, to disseminated infection involving the lungs, lymph nodes, and multiple visceral organs. Isolates of R. equi from infected goats were analyzed by polymerase chain reaction for R. equi virulence-associated plasmid ( vap) genes. Seven of 8 isolates carried the VapN plasmid, originally characterized in bovine isolates, while 1 isolate lacked virulence plasmids and was classified as avirulent. The VapN plasmid has not been described in isolates cultured from goats.

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Identification of the novel allele HLA-DRB1*150205 by polymerase chain reaction-sequence-based typing in a Chinese individual

Tissue Antigens ◽

10.1111/j.1399-0039.2009.01267.x ◽

2009 ◽

Vol 74 (2) ◽

pp. 173-175 ◽

Cited By ~ 5

Author(s):

F.-M. Zhu ◽

W. Zhang ◽

J. He ◽

L.-X. Yan

Keyword(s):

Polymerase Chain Reaction ◽

The Novel ◽

Reaction Sequence ◽

Chain Reaction ◽

Polymerase Chain ◽

Novel Allele ◽

Chinese Individual

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Extração de DNA a partir de sangue humano coagulado para aplicação nas técnicas de genotipagem de antígenos leucocitários humanos e de receptores semelhantes à imunoglobulina

Revista da Sociedade Brasileira de Medicina Tropical ◽

10.1590/s0037-86822009000600008 ◽

2009 ◽

Vol 42 (6) ◽

pp. 651-656 ◽

Cited By ~ 8

Author(s):

Daniela Maira Cardozo ◽

Gláucia Andréia Guelsin ◽

Samaia Laface Clementino ◽

Fabiano Cavalcante de Melo ◽

Marco Antônio Braga ◽

...

Keyword(s):

Polymerase Chain Reaction ◽

Killer Cell ◽

Human Leukocyte ◽

Reaction Sequence ◽

Chain Reaction ◽

Specific Sequence ◽

Salting Out ◽

Leukocyte Antigens ◽

Polymerase Chain ◽

Made In

O objetivo deste estudo foi padronizar uma metodologia de extração de DNA de alta qualidade a partir de amostras de sangue coagulado. Quarenta e oito amostras de sangue humano coagulado foram utilizadas para a extração de DNA pelo kit comercial EZ-DNA® (Biological Industries, Beit Haemek, Israel), pelo kit de coluna Neoscience® (One Lambda Inc., San Diego, CA) e pelo método modificado de salting out. Apenas o método de salting out foi capaz de extrair altas concentrações de DNA (média, 180ng/µL), as quais foram medidas pelo detector de fluorescência Qubit® (Invitrogen, USA). Este método permitiu a amplificação dos genes HLA (human leukocyte antigens) pela tecnologia PCR-SSO (polymerase chain reaction - specific sequence of oligonucleotides) Luminex, a qual exige DNA de boa qualidade, e de genes KIR (killer cell immunoglobulin-like receptors) pela técnica made in house PCR-SSP (polymerase chain reaction-sequence specific of primers), a qual demanda uma concentração específica de DNA (10ng/µL). Concluímos que a técnica de salting out modificada foi muito eficiente, simples e rápida para a extração de DNA de amostras de sangue humano coagulado, com o objetivo de realizar a genotipagem de genes HLA e KIR.

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