scholarly journals Effect of Sarsaponin on Ruminal Fermentation with Particular Reference to Methane Production in Vitro

2003 ◽  
Vol 86 (10) ◽  
pp. 3330-3336 ◽  
Author(s):  
Z.A. Lila ◽  
N. Mohammed ◽  
S. Kanda ◽  
T. Kamada ◽  
H. Itabashi
Keyword(s):  
Methane Production ◽  
Ruminal Fermentation

scholarly journals Chemical and phytochemical composition, in vitro ruminal fermentation, methane production, and nutrient degradability of fresh and ensiled Paulownia hybrid leaves

2021 ◽  
pp. 115038
Author(s):  
Haihao Huang ◽  
Malgorzata Szumacher-Strabel ◽  
Amlan Kumar Patra ◽  
Sylwester Ślusarczyk ◽  
Dorota Lechniak ◽  
...  
Keyword(s):  
Methane Production ◽  
Ruminal Fermentation ◽  
Phytochemical Composition

PSXIII-2 Effect of varying proportions of cereal rye and turnip on ruminal fermentation and methane output through in vitro batch culture

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 461-461
Author(s):  
Jordan L Cox-O’Neill ◽  
Vivek Fellner ◽  
Alan J Franluebbers ◽  
Deidre D Harmon ◽  
Matt H Poore ◽  
...  
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Ruminal Fermentation ◽  
Variable Response ◽  
Cool Season ◽  
Cereal Rye ◽  
Freeze Dried ◽  
Main Effect ◽  
Cool Season Grass

Abstract Ruminant animal performance has been variable in studies grazing annual cool-season grass and brassica monocultures and mixtures. There is little understanding of the fermentation mechanisms causing variation. The aim of this study was to determine apparent dry matter (DM) digestibility, methane, and volatile fatty acid (VFA) concentration from different proportions of cereal rye (Secale cereal; R) and turnip (Brassica rapa L.; T) (0R:100T, 40R:60T, 60R:40T, and 100R:0T) via in vitro batch fermentation. Freeze-dried forage samples from an integrated crop-livestock study was assembled into the four treatments with a 50:50 leaf to root ratio for turnip. Measurements were made following a 48 hr fermentation with 2:1 buffer and ruminal fluid inoculum. Data were analyzed using Mixed Procedure of SAS with batch (replicate) and treatment (main effect) in the model; differences were declared at P ≤ 0.05, with tendencies declared at > 0.05 but < 0.10. Rumen apparent DM digestibility (26.8%; overall mean) was not different among treatments. Methane production was less (P < 0.01) with inclusion of turnip ranging from 774 nmol/ml for 0R:100T to 1416 nmol/ml for 100R:0T. Total VFA production, acetate to propionate ratio, acetate, and valerate were not affected by forage treatments (117 mM, 1.45, 39.84 mol/100 mol, and 7.86 mol/100 mol, respectively; overall mean). Propionate, isobutyrate, and isovalerate concentrations were greater and butyrate concentration less with greater (P < 0.01) proportions of rye in the mixture. No effect of R:T ratio on digestibility or total VFA production along with the observed differences in individual VFA concentration do not explain variable response in grazing animals. Additionally, methane production results indicate that grazing turnips could potentially reduce methane production and thus reduce ruminant livestock’s contribution to greenhouse gas emissions.

scholarly journals Pomegranate seed oil rich in conjugated linolenic acids reduces in vitro methane production

1970 ◽  
Vol 46 (3) ◽  
pp. 325-335
Author(s):  
E. Maleki ◽  
G.Y. Meng ◽  
M. Faseleh Jahromi ◽  
R. Jorfi ◽  
A. Khoddami ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Seed Oil ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Ruminal Fermentation ◽  
Ch4 Production

The objective of this study was to determine the effect of pomegranate (Punica granatum L.) seed oil (PSO) on gas and methane (CH4) production, ruminal fermentation and microbial populations under in vitro conditions. Three treatments consisting of a control diet containing 10 mg tallow (CON); the control diet with 5 mg PSO + 5 mg tallow (MPSO) and the control diet containing 10 mg PSO (HPSO) were compared. Ten mg of the experimental fat/oil samples were inserted into a gas-tight 100 mL plastic syringe containing 30 mL of an incubation inoculum and 250 mg of a basic substrate of a hay/concentrate (1/1, w/w) mixture. In vitro gas production was recorded over 0, 2, 4, 6, 8, 10, 12 and 24 h of incubation. After 24 hours, incubation was stopped, and methane production, pH, volatile fatty acids (VFAs) and microbial counts were measured in the inoculant. Gas production at 4, 6, 8, 10, 12 and 24 h incubation, metabolizable energy and in vitro organic matter disappearance increased linearly and quadratically as level of PSO increased. Furthermore, the 10 mg PSO (HPSO) decreased CH4 production by 21.0% compared with the control (CON) group. There were no significant differences in total and individual VFA concentrations between different levels of PSO, except for butyric acid. After 24 h of incubation, methanogenesis decreased in the HPSO compared with the MPSO and CON treatments. In addition, total bacteria and protozoa counts increased with rising PSO levels, while population methanogenesis declined significantly. These results suggested that PSO could reduce methane emissions, which might be beneficial to nutrient utilization and growth in ruminants.

scholarly journals Flavonoids and their aromatic derivatives in Piper betle powder promote in vitro methane mitigation in a variety of diets

2020 ◽  
Vol 44 ◽  
Author(s):  
Rayudika Aprilia Patindra Purba ◽  
Siwaporn Paengkoum ◽  
Chalermpon Yuangklang ◽  
Pramote Paengkoum
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Methane Emissions ◽  
Gas Production ◽  
Piper Betle ◽  
Ruminal Fermentation ◽  
Perennial Plant ◽  
Rumen Ph ◽  
Lactating Goats

ABSTRACT At present, there is little information regarding whether supplementation with Piper betle powder (PBP) and sunflower oil (SFO) has a synergistic effect on lowering methane emissions without negatively impacting ruminal fermentation. This study investigated the effects of PBP, supplemented either with or without SFO, on biogas release, fermentation end-products, and microorganisms in the rumen of lactating goats. The treatments were run in a completely randomized 3 × 5 factorial arrangement, whereby 0, 15, and 30 mg SFO were combined with 0, 15, 30, 45, and 60 mg PBP on a dry matter basis. The outcomes were assessed in vitro. PBP was obtained from the perennial plant Piper betle L., which is an abundant source of flavonoids and their aromatic derivatives. SFO, which reduces dietary methane emissions, was supplemented to confirm whether it interacted with other nutrients in the ruminant diet. SFO × PBP significantly (p < 0.05) decreased methane production, enhanced total volatile fatty acid concentrations, and decreased the number of rumen protozoa. We found that 15-30 mg, but not 45-60 mg, PBP combined with 0, 15, and 30 mg SFO increased (p < 0.05) total gas production (including CO2) from fermentation. However, our results suggested that at least 45 mg PBP, either alone or combined with SFO, was required to reduce ammonia-N (p < 0.05). Not all treatments affected rumen pH. In conclusion, supplementing PBP (< 30 mg), either alone or combined with SFO, has a suppressing effect on methane production while preserving an optimum rate of rumen fermentation.

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