PSXIII-2 Effect of varying proportions of cereal rye and turnip on ruminal fermentation and methane output through in vitro batch culture

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 461-461
Author(s):  
Jordan L Cox-O’Neill ◽  
Vivek Fellner ◽  
Alan J Franluebbers ◽  
Deidre D Harmon ◽  
Matt H Poore ◽  
...  
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Ruminal Fermentation ◽  
Variable Response ◽  
Cool Season ◽  
Cereal Rye ◽  
Freeze Dried ◽  
Main Effect ◽  
Cool Season Grass

Abstract Ruminant animal performance has been variable in studies grazing annual cool-season grass and brassica monocultures and mixtures. There is little understanding of the fermentation mechanisms causing variation. The aim of this study was to determine apparent dry matter (DM) digestibility, methane, and volatile fatty acid (VFA) concentration from different proportions of cereal rye (Secale cereal; R) and turnip (Brassica rapa L.; T) (0R:100T, 40R:60T, 60R:40T, and 100R:0T) via in vitro batch fermentation. Freeze-dried forage samples from an integrated crop-livestock study was assembled into the four treatments with a 50:50 leaf to root ratio for turnip. Measurements were made following a 48 hr fermentation with 2:1 buffer and ruminal fluid inoculum. Data were analyzed using Mixed Procedure of SAS with batch (replicate) and treatment (main effect) in the model; differences were declared at P ≤ 0.05, with tendencies declared at > 0.05 but < 0.10. Rumen apparent DM digestibility (26.8%; overall mean) was not different among treatments. Methane production was less (P < 0.01) with inclusion of turnip ranging from 774 nmol/ml for 0R:100T to 1416 nmol/ml for 100R:0T. Total VFA production, acetate to propionate ratio, acetate, and valerate were not affected by forage treatments (117 mM, 1.45, 39.84 mol/100 mol, and 7.86 mol/100 mol, respectively; overall mean). Propionate, isobutyrate, and isovalerate concentrations were greater and butyrate concentration less with greater (P < 0.01) proportions of rye in the mixture. No effect of R:T ratio on digestibility or total VFA production along with the observed differences in individual VFA concentration do not explain variable response in grazing animals. Additionally, methane production results indicate that grazing turnips could potentially reduce methane production and thus reduce ruminant livestock’s contribution to greenhouse gas emissions.

Short Communication: Effect of condensed tannin on in vitro ruminal fermentation of purple prairie clover (Dalea purpureaVent)–cool-season grass mixture

2013 ◽  
Vol 93 (1) ◽  
pp. 155-158 ◽  
Author(s):  
L. Jin ◽  
Y. Wang ◽  
A. D. Iwaasa ◽  
Z. Xu ◽  
M. P. Schellenberg ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Dry Matter ◽  
Short Communication ◽  
Condensed Tannin ◽  
Ruminal Fermentation ◽  
Microbial Protein ◽  
Cool Season ◽  
Microbial Protein Synthesis ◽  
Cool Season Grass

Jin, L., Wang, Y., Iwaasa, A. D., Xu, Z., Schellenberg, M. P., Zhang, Y. G. and McAllister, T. A. 2013. Short Communication: Effect of condensed tannin on in vitro ruminal fermentation of purple prairie clover ( Dalea purpurea Vent)–cool- season grass mixture. Can. J. Anim. Sci. 93: 155–158. Four purple prairie clover [Dalea purpurea Vent (PPC)]–/grass mixtures containing 7.3, 14.0, 29.3 and 42.1 g kg−1dry matter (DM) of condensed tannin (CT) were incubated with rumen fluid. As the CT increased, in vitro true dry matter disappearance (IVTDMD), production of volatile fatty acids (VFA) and microbial protein synthesis were linearly increased (P<0.05) at 12 h, and IVTDMD and VFA were quadratically increased (P<0.01) at 48-h incubation. Molar proportion of acetate was linearly increased (P<0.01) but propionate and butyrate were decreased (P<0.05) at both 12 and 48-h incubation. Incorporation of PPC into cool-season grass pasture up to CT content of 42 g kg−1DM improved ruminal fermentability and microbial protein synthesis in mixed forages.

scholarly journals Flavonoids and their aromatic derivatives in Piper betle powder promote in vitro methane mitigation in a variety of diets

2020 ◽  
Vol 44 ◽  
Author(s):  
Rayudika Aprilia Patindra Purba ◽  
Siwaporn Paengkoum ◽  
Chalermpon Yuangklang ◽  
Pramote Paengkoum
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Methane Emissions ◽  
Gas Production ◽  
Piper Betle ◽  
Ruminal Fermentation ◽  
Perennial Plant ◽  
Rumen Ph ◽  
Lactating Goats

ABSTRACT At present, there is little information regarding whether supplementation with Piper betle powder (PBP) and sunflower oil (SFO) has a synergistic effect on lowering methane emissions without negatively impacting ruminal fermentation. This study investigated the effects of PBP, supplemented either with or without SFO, on biogas release, fermentation end-products, and microorganisms in the rumen of lactating goats. The treatments were run in a completely randomized 3 × 5 factorial arrangement, whereby 0, 15, and 30 mg SFO were combined with 0, 15, 30, 45, and 60 mg PBP on a dry matter basis. The outcomes were assessed in vitro. PBP was obtained from the perennial plant Piper betle L., which is an abundant source of flavonoids and their aromatic derivatives. SFO, which reduces dietary methane emissions, was supplemented to confirm whether it interacted with other nutrients in the ruminant diet. SFO × PBP significantly (p < 0.05) decreased methane production, enhanced total volatile fatty acid concentrations, and decreased the number of rumen protozoa. We found that 15-30 mg, but not 45-60 mg, PBP combined with 0, 15, and 30 mg SFO increased (p < 0.05) total gas production (including CO2) from fermentation. However, our results suggested that at least 45 mg PBP, either alone or combined with SFO, was required to reduce ammonia-N (p < 0.05). Not all treatments affected rumen pH. In conclusion, supplementing PBP (< 30 mg), either alone or combined with SFO, has a suppressing effect on methane production while preserving an optimum rate of rumen fermentation.

Methane production and in vitro digestibility of low quality forages treated with a protease or a cellulase

2016 ◽  
Vol 56 (10) ◽  
pp. 1700
Author(s):  
J. M. Cantet ◽  
D. Colombatto ◽  
G. Jaurena
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
Ruminal Fermentation ◽  
Neutral Detergent Fibre ◽  
Dry Matter Digestibility ◽  
Application Rates ◽  
The Impact

The objective was to assess the impact of application of two enzyme mixtures on the in vitro dry matter digestibility, neutral detergent fibre digestibility, net cumulative gas production and methane production after 24 h of incubation of Milium coloratum (formely Panicum coloratum) and a Patagonian meadow grassland. A protease (Protex 6-L) and a fibrolytic enzyme (Rovabio) were assessed at three application rates (30, 60 and 90 mg/100 mL of distiller water) on the substrates. Meadow samples were higher to Milium ones (P < 0.05) for in vitro dry matter digestibility and net cumulative gas production at 24 h. Nevertheless, Milium was ~11% higher than meadow (P < 0.05) for methane when expressed as a proportion of digested dry matter (g/kg). Rovabio did not induce differences in any variable, but the addition of Protex reduced (P < 0.05) in vitro dry matter digestibility in both substrates without bringing about differences in methane production. Collectively, the addition of these enzymes did not benefit in vitro ruminal fermentation of low quality forages.

scholarly journals The Effect of Different Concentrations of Total Polyphenols from Paulownia Hybrid Leaves on Ruminal Fermentation, Methane Production and Microorganisms

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2843
Author(s):  
Julia Puchalska ◽  
Małgorzata Szumacher-Strabel ◽  
Amlan Kumar Patra ◽  
Sylwester Ślusarczyk ◽  
Min Gao ◽  
...  
Keyword(s):  
Methane Production ◽  
Crude Protein ◽  
Dry Matter ◽  
Protein Concentration ◽  
Microbial Population ◽  
Ruminal Fermentation ◽  
Total Polyphenols ◽  
Methanogen Population ◽  
Hydrogen Availability

This experiment was conducted to study the effects of different concentrations of polyphenols of Paulownia Clon In Vitro 112® leaves or their particular parts on in vitro ruminal fermentation, methane production and microbial population. Paulownia leaves with high (PLH; 31.35 mg/g dry matter (DM)), medium (PLM; 26.94 mg/g DM), and low level of polyphenols (PLL; 11.90 mg/g DM) were used from three plantation areas. Lamina (PLLA; 33.63 mg/g DM) and twigs (PLT; 2.53 mg/g DM) of leaves were also collected from the PLM plantation. The chemical analyses of Paulownia leaves indicated that the content of the most basic nutrients (e.g., crude protein concentration of 185 g/kg of DM) were similar to dehydrated alfalfa. The in vitro results showed that the use of Paulownia leaves with the highest content of total polyphenols (PLH and PLLA) decreased methane production, methanogens numbers, and acetate to propionate ratio. In PLT, lowered methane production was followed by reduced substrate degradability and volatile fatty acid (VFA) concentration along with higher acetate to propionate ratio. Therefore, reduction of methane production in PLH and PLLA was attributed to the lowered methanogen population, whereas in PLT it was caused by decreased substrate degradability with the resultant of limited hydrogen availability to the methanogens.

In vitro ruminal fermentation characteristics and methane production differ in selected key pasture species in Australia

2013 ◽  
Vol 64 (9) ◽  
pp. 935 ◽  
Author(s):  
B. K. Banik ◽  
Z. Durmic ◽  
W. Erskine ◽  
K. Ghamkhar ◽  
C. Revell
Keyword(s):  
Methane Production ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
Enteric Fermentation ◽  
Rumen Microbes ◽  
Nutritive Values ◽  
The Impact ◽  
In Vitro Rumen Fermentation

Thirteen current and potential pasture species in southern Australia were examined for differences in their nutritive values and in vitro rumen fermentation profiles, including methane production by rumen microbes, to assist in selection of pasture species for mitigation of methane emission from ruminant livestock. Plants were grown in a glasshouse and harvested at 7 and 11 weeks after sowing for in vitro batch fermentation, with nutritive values assessed at 11 weeks of growth. The pasture species tested differed significantly (P < 0.001) in methane production during in vitro rumen fermentation, with the lowest methane-producing species, Biserrula pelecinus L., producing 90% less methane (4 mL CH4 g–1 dry matter incubated) than the highest methane-producing species, Trifolium spumosum L. (51 mL CH4 g–1 dry matter incubated). Proxy nutritive values of species were found not to be useful predictors of plant fermentation characteristics or methane production. In conclusion, there were significant differences in fermentative traits, including methane production, among selected pasture species in Australia, indicating that the choice of fodder species may offer a way to reduce the impact on the environment from enteric fermentation.

scholarly journals Effect of Saponaria Officinalis L. Or Panax Ginseng C.A Meyer Triterpenoid Saponins on Ruminal Fermentation in Vitro / Wpływ Saponin Triterpenowych Saponaria Officinalis L. Lub Panax Ginseng C.A. Meyer Na Przemiany Zachodzące W Żwaczu W Warunkach In Vitro

2013 ◽  
Vol 13 (4) ◽  
pp. 815-827 ◽  
Author(s):  
Joanna Szczechowiak ◽  
Małgorzata Szumacher-Strabel ◽  
Anna Stochmal ◽  
Magdalena Nadolna ◽  
Emilia Pers-Kamczyc ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Methane Production ◽  
Dry Matter ◽  
Corn Meal ◽  
Ruminal Fermentation ◽  
Triterpenoid Saponins ◽  
Potential Factor ◽  
High Forage ◽  
Saponaria Officinalis

Abstract This study examined the potential effects of Saponaria officinalis (SO) or Panax ginseng (PG) saponins supplemented to diets differing in the proportion of forage to concentrate on rumen microbial fermentation and methane production in vitro. Two experiments were carried out using the rumen simulation technique (RUSITEC). In the first experiment the substrate was comprised of a mixture of meadow hay and corn meal in a ratio of 60:40 dry matter (DM; high forage diet; HF). In the second experiment low forage diet (LF) consisting of meadow hay and corn meal in the ratio of 40:60 DM was used. Diets were supplemented with dried roots of SO or PG to provide 1% of triterpenoid saponins in dietary dry matter. All triterpenoid sources significantly (P≤0.05) decreased number of protozoa, by 50% and 72% respectively when HF diet was analysed (first experiment). There were no changes in methane production. In the second experiment (LF), the potential to mitigate methane production was reported for both SO and PG addition, however only in PG a decrease in the protozoal population was detected. Supplementation of plants rich in triterpenoid saponins has been identified as a diet dependent potential factor which has an important role in modulation of rumen fermentation processes. However, further studies are needed to evaluate their effect in animal production.

scholarly journals 127 Porcine in vitro digestibility of whole stillage predigested with multi-enzyme during different time periods

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 49-50
Author(s):  
Kevin S Jerez Bogota ◽  
Tofuko A Woyengo
Keyword(s):  
Dry Matter ◽  
In Vitro Digestion ◽  
Enzyme Treatment ◽  
Optimal Time ◽  
In Vitro Digestibility ◽  
Percentage Points ◽  
Whole Stillage ◽  
Freeze Dried ◽  
Different Sources

Abstract A study was conducted to determine the effects of the period of predigesting whole stillage (WS; slurry material that is dried into DDGS) with multi-enzyme and composition of the multi-enzyme on porcine in vitro digestibility of dry matter (IVDDM) of the WS. Four samples of whole stillage from 4 different sources were freeze-dried and divided into 13 subsamples to give 52 sub-samples. Thirteen treatments were applied to the 48 sub-samples within source. The treatments were undigested WS (control); or pre-digested with 1 of 3 multi-enzymes (MTE1, MTE2, and MTE3) at 55 °C for 6, 12, 18 or 24 h in 3 × 4 factorial arrangement. The MTE1 contained xylanase, β-glucanase, cellulase, mannanase, protease, and amylase; MTE2 contained xylanase, α-galactosidase, and cellulase; and MTE3 contained xylanase, cellulase, β-glucanase, and mannanase. The 52 subsamples were subjected to porcine in vitro digestion. The IVDDM of untreated WS was 73.3%. The IVDDM increased (P&lt; 0.05) with an increase in the predigestion period. However, a rise in the predigestion period from 0 to 12 h resulted in greater (P&lt; 0.05) response in mean IVDDM than an increment in the predigestion period from 12 to 24 h (11 vs. 0.83 percentage points). Predigestion period and multi-enzyme type interacted on IVDDM such that the improvement in IVDDM between 0 and 12 hours of predigestion differed (P&lt; 0.05) among the 3 multi-enzyme types (13.3, 11.1, and 8.5 percentage points for MTE3, MTE2, and MTE1, respectively). The LS means by multi-enzyme treatment were modeled and resulted in unparallel curves (P&lt; 0.05). The estimated maximum response of IVDDM for MTE1, MTE2 and MTE 3 were 82.4%, 84.7% and 87.1% at 15.8, 13 and 13.1 hours, respectively. In conclusion, the optimal time of predigestion of WS with multi-enzymes (with regard to improvement in its IVDDM) was approximately 14 h.

scholarly journals Chemical and phytochemical composition, in vitro ruminal fermentation, methane production, and nutrient degradability of fresh and ensiled Paulownia hybrid leaves

2021 ◽  
pp. 115038
Author(s):  
Haihao Huang ◽  
Malgorzata Szumacher-Strabel ◽  
Amlan Kumar Patra ◽  
Sylwester Ślusarczyk ◽  
Dorota Lechniak ◽  
...  
Keyword(s):  
Methane Production ◽  
Ruminal Fermentation ◽  
Phytochemical Composition
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