Bromodeoxyuridine: a comparison of its photosensitizing and radiosensitizing properties

1988 ◽  
Vol 69 (3) ◽  
pp. 410-415 ◽  
Author(s):  
Corey Raffel ◽  
Dennis F. Deen ◽  
Michael S. B. Edwards
Keyword(s):  
Uv Light ◽  
Tumor Cell Line ◽  
Single Strand ◽  
Enhancement Ratio ◽  
Dose Enhancement ◽  
Content Type ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Cross Links

✓ The photo- and radiosensitizing properties of bromodeoxyuridine (BUdR) were assessed in vitro using the 9L rat brain tumor cell line. Pretreatment of 9L cells with 10 µM BUdR for 24 hours followed by irradiation with ultraviolet (UV) light resulted in a dose-enhancement ratio of 3.8:1 compared with UV radiation alone. X-radiation of BUdR-pretreated cells produced a dose-enhancement ratio of 1.7:1. Alkaline elution analysis of deoxyribonucleic acid (DNA) from cells treated with BUdR and UV irradiation showed the presence of DNA single-strand breaks and DNA-protein cross-links. Analysis of DNA from cells treated with BUdR and then x-irradiated showed no increase in DNA single-strand breaks compared with cells treated with x-radiation alone; no DNA-protein cross-links could be detected. The possible clinical relevance of these findings is discussed.

The Equine Estrogen Metabolite 4-Hydroxyequilenin Causes DNA Single-Strand Breaks and Oxidation of DNA Bases in Vitro

1998 ◽  
Vol 11 (9) ◽  
pp. 1105-1111 ◽  
Author(s):  
Yumei Chen ◽  
Li Shen ◽  
Fagen Zhang ◽  
Serrine S. Lau ◽  
Richard B. van Breemen ◽  
...  
Keyword(s):  
Single Strand ◽  
Dna Bases ◽  
Strand Breaks ◽  
Estrogen Metabolite ◽  
Oxidation Of Dna ◽  
Single Strand Breaks

Chromosomal aberrations and deoxyribonucleic acid single-strand breaks in adipose-derived stem cells during long-term expansion in vitro

Cytotherapy ◽  
2013 ◽  
Vol 15 (7) ◽  
pp. 767-781 ◽  
Author(s):  
Katrin Froelich ◽  
Johannes Mickler ◽  
Gudrun Steusloff ◽  
Antje Technau ◽  
Mario Ramos Tirado ◽  
...  
Keyword(s):  
Stem Cells ◽  
Chromosomal Aberrations ◽  
Deoxyribonucleic Acid ◽  
Single Strand ◽  
Adipose Derived Stem Cells ◽  
Strand Breaks ◽  
Single Strand Breaks

Uranyl Acetate Causes DNA Single Strand Breaks In Vitro in the Presence of Ascorbate (Vitamin C)

2003 ◽  
Vol 16 (4) ◽  
pp. 524-530 ◽  
Author(s):  
Monica Yazzie ◽  
Shania L. Gamble ◽  
Edgar R. Civitello ◽  
Diane M. Stearns
Keyword(s):  
Vitamin C ◽  
Uranyl Acetate ◽  
Single Strand ◽  
Strand Breaks ◽  
Single Strand Breaks

Photoinduced Single Strand Breaks and Intrastrand Cross-Links in an Oligonucleotide Labeled with 5-Bromouracil

2014 ◽  
Vol 118 (19) ◽  
pp. 5009-5016 ◽  
Author(s):  
Magdalena Zdrowowicz ◽  
Barbara Michalska ◽  
Agnieszka Zylicz-Stachula ◽  
Janusz Rak
Keyword(s):  
Single Strand ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Cross Links

UV-induzierte Brüche in 5-Bromuracil-substituierter DNS des Phagen T1 / UV-Induced Strand Breaks in 5-Bromouracil Substituted DNA of Phage T1

1970 ◽  
Vol 25 (9) ◽  
pp. 1037-1042 ◽  
Author(s):  
G. Stephan ◽  
H. G. Miltenburger ◽  
G. Hotz
Keyword(s):  
Biological Activity ◽  
Uv Irradiation ◽  
Uv Light ◽  
Double Strand Breaks ◽  
Single Strand ◽  
Radical Scavenger ◽  
Strand Breaks ◽  
Single Strand Breaks ◽  
Phage Dna ◽  
Site Of Action

Phage Tl and BU-Τ1 (phage DNA substituted with 5-bromouracil) were inactivated by UV-light (2537 A). After irradiation DNA was extracted and its sedimentation behaviour studied by zone centrifugation in neutral and alkaline sucrose gradients, respectively. In the DNA of unsubstituted phage no double-strand breaks and only a small number of single-strand breaks per inactivation dose could be detected after high UV-doses. However, after irradiation of phage BU-T1 double- and single-strand breaks were observed at an increased rate. UV-irradiation in the presence of a radical scavenger (0.01 M cysteamine) prevented the occurrence of double- but not of single strand breaks. The relevance of these breaks attributable to BU-incorporation for the biological activity (plaque forming ability) of the phage and the site of action of cysteamine are discussed.

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