Determination of Lactate Kinetics in the Human Analysis of Data From Single Injection vs. Continuous Infusion Methods

1972 ◽  
Vol 139 (3) ◽  
pp. 1002-1006 ◽  
Author(s):  
G. L. Searle ◽  
R. R. Cavalieri
Keyword(s):  
Continuous Infusion ◽  
Single Injection ◽  
Lactate Kinetics

Measurement of leucine metabolism in man from a primed, continuous infusion of L-[1-3C]leucine

1980 ◽  
Vol 238 (5) ◽  
pp. E473-E479 ◽  
Author(s):  
D. E. Matthews ◽  
K. J. Motil ◽  
D. K. Rohrbaugh ◽  
J. F. Burke ◽  
V. R. Young ◽  
...  
Keyword(s):  
Steady State ◽  
Continuous Infusion ◽  
Co2 Production ◽  
Human Beings ◽  
Priming Dose ◽  
Leucine Metabolism ◽  
Kinetics Of ◽  
13Co2 Enrichment

Leucine metabolism in vivo can be determined from a primed, continuous infusion of L-[1-13C]leucine by measuring, at isotopic steady state, plasm [-13C]leucine enrichment, expired 13CO2 enrichment, and CO2 production rate. With an appropriate priming dose of L-[1-13C]leucine and NaH13CO3, isotopic steady state is reached in less than 2 h, and the infusion is completed in 4 h. The method can determine rates of leucine turnover, oxidation, and incorporation into protein with typical relative uncertainties of 2, 10, and 4%, respectively. The method requires no more than 1 ml of blood and uses stable isotope rather than radioisotope techniques. Thus, the method is applicable to studies of human beings of all ages. L-[1-13C]leucine may be infused with a second amino acid labeled with 15N for simultaneous determination of the kinetics of two amino acids.

Calculator assisted determination of dilutions for continuous infusion ICU medications

1982 ◽  
Vol 10 (9) ◽  
pp. 610-612 ◽  
Author(s):  
JOSEF NEU ◽  
COLLEEN MAHONEY ◽  
ALLEN D. WILSON ◽  
THOMAS B. RICE
Keyword(s):  
Continuous Infusion

scholarly journals Simultaneous determination of parabens and inorganic anions in cosmetics by a two-dimensional ultrahigh-performance liquid chromatography-ion chromatography valve-switching method

RSC Advances ◽  
2017 ◽  
Vol 7 (52) ◽  
pp. 32769-32776 ◽  
Author(s):  
Feng-Lian Wang ◽  
Yong-Gang Zhao ◽  
Nadeem Muhammad ◽  
Shu-Chao Wu ◽  
Yan Zhu
Keyword(s):  
Simultaneous Determination ◽  
Single Injection ◽  
Inorganic Anions ◽  
Two Dimensional ◽  
Switching System ◽  
System P ◽  
Ultrahigh Performance Liquid Chromatography ◽  
Switching Method ◽  
Valve Switching

Simultaneous determination of parabens and inorganic anions in cosmetics in a single injection was obtained in a two-dimensional UHPLC-IC valve switching system.

Isolation of radioactive iodothyronines for kinetic studies: a comparison of two methods

1982 ◽  
Vol 99 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Jens Faber ◽  
lb Bo Lumholtz ◽  
Carsten Kirkegaard ◽  
Kaj Siersbæk-Nielsen ◽  
Thorkild Friis
Keyword(s):  
Radioactive Iodine ◽  
Single Injection ◽  
Kinetic Studies ◽  
Acid Precipitation ◽  
Relative Difference ◽  
Metabolic Clearance ◽  
Metabolic Clearance Rate ◽  
Minor Degree ◽  
A Minor

Abstract. A method based on the principle of gel separation followed by antibody extraction (GSAE) has been developed for isolation of radioactive thyroxine (T4), 3,5,3'-triiodothyronine (T3), 3,3',5'-triiodothyronine (rT3), 3,3'-diiodothyronine (3,3'-T2), 3',5'-diiodothyronine (3',5'-T2) and 3'-monoiodothyronine (3'-T1) in serum. This method was used for the estimation of the metabolic clearance rate (MCR) of the iodothyronines using the single injection, non-compartmental approach, and was compared to the conventional trichloroacetic acid precipitation/ethanol extraction (TCA-E) technique. The GSAE method excluded the co-determination of radioactive iodine and iodoproteins, whereas the co-determination of radiolabelled daughter iodothyronines was found negligible. The relative difference of duplicate estimations of MCR was approximately 10%. Using the TCA-E method for isolation of tracer, the MCR of T4, T3 and rT3 was underestimated to a minor degree (20%), whereas the MCRs of 3,3'-T2, 3',5'-T2 and 3'-T1 were 20–40% of those estimated by the GSAE method. In conclusion the GSAE method was found suitable for kinetic studies of iodothyronines, whereas the TCA-E method cannot be used for turnover studies of 3,3'-T2, 3',5'-T2 or 3'-T1.

scholarly journals Determination of Lymphocyte Turnover by Continuous Infusion of H3 Thymidine

Blood ◽  
1962 ◽  
Vol 19 (2) ◽  
pp. 236-242 ◽  
Author(s):  
J. R. LITTLE ◽  
GEORGE BRECHER ◽  
T. R. BRADLEY ◽  
S. ROSE
Keyword(s):  
Continuous Infusion ◽  
Peripheral Blood ◽  
Osmotic Pump ◽  
Initial Delay

Abstract The use of an osmotic pump for continuous infusion of H3 thymidine in rats is described. Estimates of turnover times of cells in the peripheral blood are 4 days for granulocytes, 60 days for large lymphocytes and in excess of 100 days for small lymphocytes. These turnover times include initial delay in release or maturation after the last mitosis in the marrow or nodes, and any time spent outside the circulation prior to final disappearance of a given cell from the peripheral blood. The data exclude reutilization as an explanation for the observed longevity of lymphocytes.

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