Biotransformation of Androst-4-Ene-3,17-Dione by Some Fungi

2017 ◽  
Vol 41 (10) ◽  
pp. 594-597 ◽  
Author(s):  
Kudret Yildirim ◽  
Ali Kuru ◽  
Ece Keskin ◽  
Aylin Salihoglu ◽  
Neslihan Bukum
Keyword(s):  
Mucor Hiemalis ◽  
Aspergillus Tamarii ◽  
Monooxygenase Activity ◽  
Aspergillus Wentii ◽  
Aspergillus Candidus

The incubations of androst-4-ene-3,17-dione with Aspergillus candidus MRC 200634, Aspergillus tamarii MRC 72400, Aspergillus wentii MRC 200316 and Mucor hiemalis MRC 70325 for 5 days are reported. A. candidus MRC 200634 mainly hydroxylated androst-4-ene-3,17-dione at C-11α, C-15α and C-15β whilst A. wentii MRC 200316 hydroxylated it mainly at C-6β. A. tamarii MRC 72400 showed predominately a Baeyer–Villiger monooxygenase activity. M. hiemalis MRC 70325 hydroxylated the substrate at C-14α and reduced most of it at C-17.

scholarly journals Identifikasi Jamur Kontaminan yang Bersifat Xerofilik pada Lada Bubuk

Biomedika ◽  
2018 ◽  
Vol 10 (2) ◽  
pp. 25-30
Author(s):  
Santika Widowati ◽  
Kartinah W. ◽  
Guruh Sri Pamungkas
Keyword(s):  
Aspergillus Niger ◽  
Aspergillus Fumigatus ◽  
Aspergillus Oryzae ◽  
Aspergillus Ochraceus ◽  
Aspergillus Tamarii ◽  
Aspergillus Candidus

Lada merupakan salah satu jenis rempah yang cukup penting, baik ditinjau dari peranannya sebagai penyumbang devisa negara maupun kegunaannya yang khas dan tidak dapat digantikan oleh jenis rempah lainnya. Pada proses pengolahan lada yang masih dilakukan dengan cara yang kurang higienis, risiko produk terkontaminasi oleh jamur selama proses pengolahan lada bubuk masih sangat besar, khususnya jamur xerofilik, yaitu jamur yang mampu tumbuh pada produk kering atau kadar air yang rendah. Penelitian ini bertujuan untuk mengetahui adanya jamur xerofilik pada lada bubuk dan mengetahui spesies jamur xerofilik apa saja yang berpotensi sebagai penghasil toksin pada lada bubuk. Metode yang digunakan untuk pemeriksaan yaitu metode taburan. Sampel lada bubuk di encerkan dengan menggunakan aquades steril, dengan pengenceran 10-1, 10-2, dan 10-3, masing-masing di inokulasikan ke dalam medium DG18 pada cawan petri steril dan diinkubasi selama 5-7 hari. Kemudian di identifikasi secara makroskopis dan mikroskopis. Hasil identifikasi menunjukan lada bubuk terkontaminasi jamur xerofilik. Spesies jamur xerofilik yang terdapat pada lada bubuk yaitu Aspergillus candidus, Aspergillus ochraceus, Aspergillus fumigatus, Eurotium herbariorum, Aspergillus tamarii, Eurotium chevalieri, Aspergillus penicilloides, Aspergillus niger, dan Aspergillus oryzae. Dari 9 spesies jamur yang ditemukan, 6 diantaranya dapat berpotensi sebagai penghasil mikotoksin.

Aspergillus sydowii Strain SCAU066 and Aspergillus versicolor Isolate BAB-6580: Potential Source of Xylanolytic, Cellulolytic and Amylolytic Enzymes

2020 ◽  
Vol 14 (2) ◽  
pp. 15
Author(s):  
Zaidah Zainal ariffin
Keyword(s):  
Extracellular Enzymes ◽  
Biologically Active ◽  
Aspergillus Versicolor ◽  
Aspergillus Sydowii ◽  
Aspergillus Tamarii ◽  
Alternative Source ◽  
Amylolytic Enzymes ◽  
Potato Dextrose Broth ◽  
Wide Range ◽  
Pharmaceutical Industries

Fungi is known to produce a wide range of biologically active metabolites and enzymes. Enzymes produced by fungi are utilized in food and pharmaceutical industries because of their rich enzymatic profile. Filamentous fungi are particularly interesting due to their high production of extracellular enzymes which has a large industrial potential. The aim of this study is to isolate potential soil fungi species that are able to produce functional enzymes for industries. Five Aspergillus species were successfully isolated from antibiotic overexposed soil (GPS coordinate of N3.093219 E101.40269) by standard microbiological method. The isolated fungi were identified via morphological observations and molecular tools; polymerase chain reactions, ITS 1 (5’- TCC GTA GGT GAA CCT GCG G3’) forward primer and ITS 4 (5’-TCC TCC GCT TAT TGA TAT GC-3’) reverse primer. The isolated fungi were identified as Aspergillus sydowii strain SCAU066, Aspergillus tamarii isolate TN-7, Aspergillus candidus strain KUFA 0062, Aspergillus versicolor isolate BAB-6580, and Aspergillus protuberus strain KAS 6024. Supernatant obtained via submerged fermentation of the isolated fungi in potato dextrose broth (PDB) and extracted via centrifugation was loaded onto specific media to screen for the production of xylanolytic, cellulolytic and amylolytic enzymes. The present findings indicate that Aspergillus sydowii strain SCAU066 and Aspergillus versicolor isolate BAB-6580 have great potential as an alternative source of xylanolytic, cellulolytic and amylolytic enzymes.

scholarly journals Methanobactin from Methylocystis sp. Strain SB2 Affects Gene Expression and Methane Monooxygenase Activity in Methylosinus trichosporium OB3b

2015 ◽  
Vol 81 (7) ◽  
pp. 2466-2473 ◽  
Author(s):  
Muhammad Farhan Ul-Haque ◽  
Bhagyalakshmi Kalidass ◽  
Alexey Vorobev ◽  
Bipin S. Baral ◽  
Alan A. DiSpirito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Methane Monooxygenase ◽  
Particulate Methane Monooxygenase ◽  
Methylosinus Trichosporium Ob3b ◽  
Methylosinus Trichosporium ◽  
Monooxygenase Activity ◽  
Content Type ◽  
Soluble Methane Monooxygenase ◽  
A Subunit ◽  
Membrane Bound

ABSTRACTMethanotrophs can express a cytoplasmic (soluble) methane monooxygenase (sMMO) or membrane-bound (particulate) methane monooxygenase (pMMO). Expression of these MMOs is strongly regulated by the availability of copper. Many methanotrophs have been found to synthesize a novel compound, methanobactin (Mb), that is responsible for the uptake of copper, and methanobactin produced byMethylosinus trichosporiumOB3b plays a key role in controlling expression of MMO genes in this strain. As all known forms of methanobactin are structurally similar, it was hypothesized that methanobactin from one methanotroph may alter gene expression in another. WhenMethylosinus trichosporiumOB3b was grown in the presence of 1 μM CuCl2, expression ofmmoX, encoding a subunit of the hydroxylase component of sMMO, was very low.mmoXexpression increased, however, when methanobactin fromMethylocystissp. strain SB2 (SB2-Mb) was added, as did whole-cell sMMO activity, but there was no significant change in the amount of copper associated withM. trichosporiumOB3b. IfM. trichosporiumOB3b was grown in the absence of CuCl2, themmoXexpression level was high but decreased by several orders of magnitude if copper prebound to SB2-Mb (Cu-SB2-Mb) was added, and biomass-associated copper was increased. Exposure ofMethylosinus trichosporiumOB3b to SB2-Mb had no effect on expression ofmbnA, encoding the polypeptide precursor of methanobactin in either the presence or absence of CuCl2.mbnAexpression, however, was reduced when Cu-SB2-Mb was added in both the absence and presence of CuCl2. These data suggest that methanobactin acts as a general signaling molecule in methanotrophs and that methanobactin “piracy” may be commonplace.

Aspergillus candidus eumycetoma with review of literature

2021 ◽  
pp. 101135
Author(s):  
Manharpreet Kaur ◽  
Nidhi Singla ◽  
Mala Bhalla ◽  
Reetu Kundu ◽  
Neelam Gulati ◽  
...  
Keyword(s):  
Review Of Literature ◽  
Aspergillus Candidus

Terphenyl derivatives and terpenoids from a wheat-born mold Aspergillus candidus

2019 ◽  
Vol 73 (3) ◽  
pp. 189-193
Author(s):  
JunJie Han ◽  
FangMing Lu ◽  
Li Bao ◽  
HanYing Wang ◽  
BaoSong Chen ◽  
...  
Keyword(s):  
Aspergillus Candidus
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