scholarly journals Identifikasi Jamur Kontaminan yang Bersifat Xerofilik pada Lada Bubuk

Biomedika ◽  
2018 ◽  
Vol 10 (2) ◽  
pp. 25-30
Author(s):  
Santika Widowati ◽  
Kartinah W. ◽  
Guruh Sri Pamungkas

Lada merupakan salah satu jenis rempah yang cukup penting, baik ditinjau dari peranannya sebagai penyumbang devisa negara maupun kegunaannya yang khas dan tidak dapat digantikan oleh jenis rempah lainnya. Pada proses pengolahan lada yang masih dilakukan dengan cara yang kurang higienis, risiko produk terkontaminasi oleh jamur selama proses pengolahan lada bubuk masih sangat besar, khususnya jamur xerofilik, yaitu jamur yang mampu tumbuh pada produk kering atau kadar air yang rendah. Penelitian ini bertujuan untuk mengetahui adanya jamur xerofilik pada lada bubuk dan mengetahui spesies jamur xerofilik apa saja yang berpotensi sebagai penghasil toksin pada lada bubuk. Metode yang digunakan untuk pemeriksaan yaitu metode taburan. Sampel lada bubuk di encerkan dengan menggunakan aquades steril, dengan pengenceran 10-1, 10-2, dan 10-3, masing-masing di inokulasikan ke dalam medium DG18 pada cawan petri steril dan diinkubasi selama 5-7 hari. Kemudian di identifikasi secara makroskopis dan mikroskopis. Hasil identifikasi menunjukan lada bubuk terkontaminasi jamur xerofilik. Spesies jamur xerofilik yang terdapat pada lada bubuk yaitu Aspergillus candidus, Aspergillus ochraceus, Aspergillus fumigatus, Eurotium herbariorum, Aspergillus tamarii, Eurotium chevalieri, Aspergillus penicilloides, Aspergillus niger, dan Aspergillus oryzae. Dari 9 spesies jamur yang ditemukan, 6 diantaranya dapat berpotensi sebagai penghasil mikotoksin.

Author(s):  
O. Y. Aguda ◽  
S. O. Bankole ◽  
E. A. Adekunle ◽  
O. I. Bolanle-Ojo

Aframomum species (Aframomum danielli, Aframomum melegueta and Aframomum sceptrum) are used traditionally as medicine and food preservatives. Synthetic preservatives have been reported to be carcinogenic; hence, the continuous search for a natural preservative. This study was designed to validate the efficacy of the three named Aframomum species as a preservative against fungi that causes spoilage in raw fish and meat. The methanolic extracts of the samples were screened against Aspergillus tamarii, Aspergillus fumigatus, Aspergillus ochraceus and Trichoderma sp. using pour plate technique. The fungi were isolated from the raw fish and meat by the method of serial dilution then pour plated into Potatoes Dextro Agar (PDA) incubated at 37ºC for 7 days. The fungi observed were subcultured to get pure culture. The three samples showed significant antifungal activities against Aspergillus tamarii, Aspergillus fumigatus, Aspergillus ochraceus and Trichoderma sp. at 25%, 50% and 75% concentrations. The significant antifungal activities displayed by extract of these samples could be attributed to their phytochemical and nutritional components of the samples as well as their antioxidant activity. The three samples could be valuable natural preservatives with additional therapeutic potential.


2011 ◽  
Vol 35 (1) ◽  
pp. 167-173
Author(s):  
Rusol Muhammedi Al Bahran

The study included 100 samples collected from different locations of the homes were located in the area of Ali Saleh in Baghdad 6 species were isolated from fungi and the most common genus or species of fungi isolated were Aspergillus fumigatus by frequency ratio of 25.84%, and occurrence ratio of 23%, Penicilium by frequency ratio of 21.34%, and occurrence ratio of 19%, Mucor by frequency ratio 20.22%, and the occurrence ratio of 18%, Candida albicans by frequency ratio of 15.73%, the occurrence ratio of 14%, Rhizopus frequency ratio by 13.48%, the occurrence ratio of 12% and Aspergillus niger frequency ratio by 3.37% and the occurrence ratio of 3%. Then the sensitivity test of disinfectants were studied against fungi isolated by using three disinfectants Chloroxylenol known commercially by (Dettol), Chlorhexidine commercially known by (Hibitane) and Sodium hypochlorite commercially known by (Bleach), and a study for the effected of three concentrations of each disinfectant (5, 2.5, 1.25)%, and the use of statistical analysis (ANOVA) to contrast the differences and Dnken test to the variation in any disinfectant or the most efficient concentrations of other disinfectants were observed that concentrations of 5% was the most efficient of concentrations than (2.5%) and (1.25%). As the disinfectant Dettol was significantly the most efficient from Bleach and Hibitane.


Author(s):  
سعاد محمد خليفة أبوالغيث ◽  
أحلام القمودي محمد زعيط

استهدفت هذه الدراسة عزل بعض أنواع الفطريات من التربة الملوثة بالهيدروكربون بمصفاة الزاوية لتكرير النفط، حيث تم عزل وتعريف بعض الفطريات مثل Rhizopus, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus, Aspergillus nidulans وأوضحت نتائج هذه الدراسة أن تواجد وتنوع فطر Aspergillus قد تفوق معنويا مقارنة بتواجد وتنوع فطرRhizopus. كما تم في هذه الدراسة اختبار قدرة وكفاءة الفطريات المعزولة على النمو واستغلال المركبات الهيدروكربونية المتمثلة في زيت الحمادة وزيت الشرارة بتركيز 1% و3%، حيث أوضحت النتائج بأن جنس Rhizopus سجل أعلى معدل للنمو على الوسط الغذائي Malt Extract Agar، وسجل كلا من فطر A. fumigatus وفطر A. flavus معدّل النمو القطري أعلى معنويا من النمو القطري لفطرA. niger  وفطر A. nidulans. هذه المعدّلات العالية تدل على إمكانية استخدام الفطريات المعزولة في المعالجة البيولوجية للتربة الملوّثة بالنفط.


Author(s):  
Kassim Dekhil

 Objective: This study was aimed to identify the public pattern of presentation, influencing factors, and sort the fungal species, distribution of sex of patients with otomycosis.Results: The predominant complaints were pruritus and found in 76 patients (88.73%), discomfort and pain found in 62 patients (72.09%), aural fullness in 48 patients (55.81%), tinnitus in 34 patients (39.53%), hearing impairment in 50 cases (58.31%), ear discharge in 22 patients (25.58%), and most of the symptoms seen in 36 patients (68.14%). The results showed a total of eight fungal species belong to six different genera, namely, Aspergillus, Candida, Penicillium, Rhizopus, Alternaria, and Cephalosporium were isolated during this study. Among identified fungi, Aspergillus niger was found to be the most prevalent fungal species with 35.71% followed by Candida albicans (27.55%), Aspergillus flavus (10.20%), Aspergillus fumigatus (8.16), Penicillium digitatum (6.12%) and Cephalosporium species (4.08%), and Rhizopus species (5.1%), while Alternaria alternata had the lowest percentage (6.54%).Conclusion: Otomycosis/mycotic otitis externa is still a common problem and there is a rise in the occurrence of otomycosis in latest years, especially in tropical and subtropical humid climates.


1990 ◽  
Vol 36 (9) ◽  
pp. 625-630 ◽  
Author(s):  
P. Vanacker ◽  
B. Bacle ◽  
G. Vidal ◽  
L. Lacoste

We have searched for producers of a saccharifying activity with improved thermostability compared with industrial amyloglucosidases. These producers were chosen among thermophilic, thermotolerant, or even mesophilic fungi. Among the 846 isolated strains, five species (two Thermoascus spp., a member of the Aspergillus fumigatus group, and two members of the Aspergillus niger group) showed an amyloglucosidasic complex with the required property. Whereas the first three of these were thermophilic or thermotolerant strains, the latter two strengthen the idea that mesophilic strains can produce thermostable enzymes. The thermostability of the saccharifying complex of the Thermoascus spp., established with a half-life measure, was found to be far better than the other ones. The industrial use of these strains was discussed, and Thermoascus crustaceus seems to be the most advantageous one. Key words: filamentous fungi, amyloglucosidases, thermostability, Thermoascus.


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