Association of MMP-9 and PCNA Protein Expression in Osteosarcoma are Associated with Clinical Stage, Metastasis, and Prognosis

ABSTRACT Present work studies the expressions of MMP-9 and PCNA in osteosarcoma patients and its correlation with clinical stage. 53 specimens of osteosarcoma were surgically removed and 28 osteochondroma tissues were used for immunohistochemical staining to detect the expression of proliferating cell nuclear antigen (PCNA) as well as matrix metalloproteinase-9 (MMP-9). The expression rates of MMP-9, as well as PCNA, were 75.5 percent and 86.8 percent in osteosarcoma, while were 10.7 percent and 7.5 percent in osteochondroma, respectively, which was statistically significant (P<0.05). The expression levels of MMP-9, PCNA, and the total survival time were connected with the Enneking stage. It shows an upward trend for the expression levels of PCNA and MMP- 9 in osteosarcoma tissues. The expression levels of MMP-9 and PCNA are related to the clinical stage, metastasis, and prognosis.

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EXPRESSION OF EPIDERMAL GROWTH-FACTOR (EGF), MATRIX METALLOPROTEINASE-9 (MMP-9) AND PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) IN ESOPHAGEAL CANCER

International Journal of Oncology ◽

10.3892/ijo.6.4.833 ◽

1995 ◽

Cited By ~ 1

Author(s):

I SHIMA ◽

Y SASAGURI ◽

N ARIMA ◽

H YAMANA ◽

H FUJITA ◽

...

Keyword(s):

Esophageal Cancer ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Matrix Metalloproteinase ◽

Proliferating Cell Nuclear Antigen ◽

Matrix Metalloproteinase 9 ◽

Nuclear Antigen ◽

Epidermal Growth ◽

Proliferating Cell ◽

Metalloproteinase 9

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Proliferating cell nuclear antigen and mitotic activity in rectal cancer: predictor of response to preoperative irradiation.

Journal of Clinical Oncology ◽

10.1200/jco.1994.12.4.679 ◽

1994 ◽

Vol 12 (4) ◽

pp. 679-682 ◽

Cited By ~ 20

Author(s):

C G Willett ◽

G Warland ◽

R Cheek ◽

J Coen ◽

J Efird ◽

...

Keyword(s):

Rectal Cancer ◽

Mitotic Activity ◽

Proliferating Cell Nuclear Antigen ◽

Residual Disease ◽

Tumor Regression ◽

Rectal Wall ◽

Clinical Stage ◽

Nuclear Antigen ◽

Preoperative Irradiation ◽

Proliferating Cell

PURPOSE This study examines the association between the pathologic response of rectal cancer after irradiation and its pretreatment proliferative state as assayed by proliferating cell nuclear antigen (PCNA) and mitotic activity. PATIENTS AND METHODS Ninety patients with clinical stage T3 and T4 rectal cancer received preoperative irradiation followed by surgery. Pretreatment tumor biopsies were scored for PCNA activity (number of tumor cells staining immunohistochemically with an anti-PCNA monoclonal antibody) and the number of mitoses per 10 high-powered fields (hpf). Postirradiation surgical specimens were examined for extent of residual disease. RESULTS The tumors of 33 of 90 patients (37%) exhibited marked pathologic downstaging (no residual tumor or cancer confined to the rectal wall) after preoperative irradiation. Two features were independently associated with the likelihood of marked pathologic regression after preoperative irradiation: lesion size and PCNA/mitotic activity. When stratified by tumor size, marked tumor regression occurred most frequently in smaller tumors with high PCNA/mitotic activity compared with larger tumors with lower PCNA/mitotic activity. Intermediate downstaging rates were seen for small or large tumors with moderate PCNA/mitotic activity. CONCLUSION Tumor PCNA/mitotic activity predicts the likelihood of response to irradiation, which may aid in formulating treatment policies for patients with rectal cancer.

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Effect of natural antioxidants and apocynin on LPS-induced endotoxemia in rabbit

Human & Experimental Toxicology ◽

10.1191/096032700666138364 ◽

2000 ◽

Vol 19 (11) ◽

pp. 604-614 ◽

Cited By ~ 16

Author(s):

V Ben-Shaul ◽

L Lomnitskil ◽

A Nyska ◽

M Carbonatto ◽

S Peano ◽

...

Keyword(s):

Nadph Oxidase ◽

Proliferating Cell Nuclear Antigen ◽

Immunohistochemical Staining ◽

Microsomal Fraction ◽

Natural Antioxidants ◽

Treated Group ◽

Nuclear Antigen ◽

Sod Activity ◽

Proliferative Effect ◽

Proliferating Cell

The objective of this study was to compare the prophylactic effects of the natural antioxidant from spinach (NAO) and apocynin, on the hepatic oxidative stress and liver damage induced by lipopolysaccharide (LPS). Male New Zealand rabbits were challenged with LPS with or without 8 days of antioxidant pretreatment. Pretreatment with NAO, but not apocynin, significantly (p<0.05) decreased the levels of hydroperoxides and malondialdehyde (MDA) in the liver cytosolic fraction and the activity of NADPH oxidase-generated superoxide in the microsomal fraction, compared to LPS alone. The activity of glutathione peroxidase (G-POX) was significantly (p<0.05) increased in the LPS-treated group, whereas treatment with NAO, but not apocynin, significantly (p< 0.05) decreased G-POX activity. Pretreatment with the same antioxidants had no significant effects on superoxide dismutase (SOD) activity, whereas an increased level of catalase (CAT) was obtained in all LPS-treated groups. TUNEL immunohistochemical staining in the LPS-treated animals indicated that there was no increase in apoptosis outside of necrotic foci. However, apoptotic hepatocytes were observed within areas of focal necrosis in animals exposed to LPS alone or LPS plus apocynin. Hepatocyte cell proliferation was tested by the proliferating-cell nuclear antigen (PCNA) tool, which indicated a proliferative effect in the LPS group, whereas the effect disappeared in the antioxidant-treated groups. The prophylactic effect of NAO on liver pathology and the significant decreases in lipid peroxidation products and NADPH oxidase activity suggest the use of NAO as an efficient strategy for treatanent of endotoxemia.

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