Penghambatan berbagai isolat Trichoderma sp. terhadap perkecambahan spora Colletotrichum sp.

Danar Wicaksono; Maftuh Kafiya

doi:10.31943/agrowiralodra.v5i1.74

Penghambatan berbagai isolat Trichoderma sp. terhadap perkecambahan spora Colletotrichum sp.

Agro Wiralodra ◽

10.31943/agrowiralodra.v5i1.74 ◽

2022 ◽

Vol 5 (1) ◽

pp. 20-27

Author(s):

Danar Wicaksono ◽

Maftuh Kafiya

Keyword(s):

Trichoderma Sp

Penyakit antraknosa pada buah cabai menyebabkan kualitas hasil menurun dan kehilangan hasil yang tinggi. Penyakit ini disebabkan oleh jamur Colletotrichum sp. Pada umumnya, antraknosa pada cabai dikendalikan dengan aplikasi fungisida kimia sintetis pada seluruh permukaan buah. Hal ini sangat berbahaya bila dilakukan menjelang panen karena buah cabai juga dikonsumsi segar. Agens pengendali hayati merupakan alternatif pengendalian Colletotrichum sp. pada buah cabai. Penelitian bertujuan untuk memperoleh isolat agens pengendali hayati yang dapat menghambat perkecambahan spora Colletotrichum sp. penyebab penyakit antraknosa pada buah cabai besar keriting. Penelitian diawali dengan isolasi Colletotrichum sp. dari cabai rawit, besar, dan merah keriting. Isolasi menghasilkan dua buah isolat dari cabai merah besar, 5 isolat diisolasi dari cabai merah keriting, dan 7 isolat dari cabai rawit. Uji virulensi dilakukan untuk memperoleh isolat yang paling virulen terhadap cabai merah keriting. Isolat BA asal cabai besar diketahui memiliki virulensi paling tinggi dibandingkan yang lainnya. Tujuh Trichoderma, 1 Gliocladium, dan 2 APH yang belum diidentifikasi digunakan biakan dalam media cair. Suspensi APH digunakan untuk mengendalikan penyakit antraknosa pada buah cabai merah keriting. Penambahan suspensi LPT2 menyebabkan persentase perkecambahan paling sedikit.

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PENGARUH PUPUK ORGANIK CAIR DAN Trichoderma sp TERHADAP PERTUMBUHAN DAN HASIL MELON (Cucumis melo)

Jurnal BiBieT ◽

10.22216/jbbt.v3i1.3298 ◽

2018 ◽

Vol 3 (1) ◽

pp. 17

Author(s):

Lena Ananda Putri ◽

Jamillah Jamillah ◽

Widodo Haryoko

Keyword(s):

Cucumis Melo ◽

Organic Fertilizer ◽

Fresh Fruit ◽

Growth And Yield ◽

Randomized Design ◽

Trichoderma Sp

Percobaan “Pengaruh Pupuk Organik Cair (POC) dan Trichoderma sp Terhadap Pertumbuhan dan Hasil Melon (Cucumis melo) telah dilaksanakan di Kelurahan Air Pacah, Kecamatan Koto Tangah di Kota Padang. Tujuan penelitian ini adalah untuk mengetahui pengaruh pupuk organik cair dan Trichoderma sp terhadap pertumbuhan dan hasil Melon (Cucumis melo). Percobaan ini menggunakan rancangan acak lengkap 2 faktor (Trichoerma sp dan POC). Trichoderma sp terdiri dari 2 tingkat yaitu 0 g L-1 dan 200 g L-1 per tanaman, sedangkan POC pemberian terdiri dari 4 tingkat yaitu 0 ml L-1, 25 ml L-1, 50 ml L-1 dan 75 ml L-1 dengan 3 ulangan dan setiap satuan percobaan 2 polibeg sehingga diperoleh 48 polibag. Dari hasil percobaan disimpulkan bahwa pemberian 200 gram inokulan Trichoderma sp dan 75 ml L-1 POC yang diberikan setiap 2 minggu mampu meningkatkan pertumbuhan dan hasil tertinggi berat buah segar mencapai 1,33 kg/buah Experiment "The Effect of Liquid Organic Fertilizer (LOF) and Trichoderma sp Against Growth and Melon Yield (Cucumis melo L) has been conducted in Air Pacah Koto Tangah Subdistrict in Padang City. The purpose of this research is to know the effect of liquid organic fertilizer and Trichoderma sp on growth and yield of Melon (Cucumis melo L). This study used a complete randomized design of 2 factors (Trichoderma sp and POC). Trichoderma sp consists of 2 levels of 0 g L-1 and 200 g L-1 per plant, whereas POC administration consists of 4 levels ie 0 ml L-1, 25 ml L-1, 50 ml L-1 and 75 ml L-1 with 3 replications and each unit of experiment 2 polybag so obtained 48 polybags. The experimental resulted that giving 200 grams of Trichoderma sp inoculum and 75 ml L-1 POC was given every 2 weeks can increase the growth and yield of melon. The highest yield of fresh fruit 1.33 kg/fruit.

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Production of β‐galactosidase by Trichoderma sp. through solid‐state fermentation targeting the recovery of galactooligosaccharides from whey cheese

Journal of Applied Microbiology ◽

10.1111/jam.14805 ◽

2020 ◽

Author(s):

L.F.M.C. Jesus ◽

L.H.S. Guimarães

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Trichoderma Sp

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Growth and Reproduction of Trichoderma sp. in with presence Bacillus sp. or Fluorescent Pseudomonad

Journal of Physics Conference Series ◽

10.1088/1742-6596/1899/1/012021 ◽

2021 ◽

Vol 1899 (1) ◽

pp. 012021

Author(s):

Gita Pawana ◽

Kaswan Badami ◽

and Achmad Djunaedy

Keyword(s):

Fluorescent Pseudomonad ◽

Bacillus Sp ◽

Trichoderma Sp ◽

Growth And Reproduction

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Extracellular α-Galactosidase from Trichoderma sp. (WF-3): Optimization of Enzyme Production and Biochemical Characterization

Biotechnology Research International ◽

10.1155/2015/860343 ◽

2015 ◽

Vol 2015 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Aishwarya Singh Chauhan ◽

Arunesh Kumar ◽

Nikhat J. Siddiqi ◽

B. Sharma

Keyword(s):

Enzyme Activity ◽

Enzyme Assay ◽

Biochemical Characterization ◽

Catalytic Efficiency ◽

Culture Conditions ◽

Significant Loss ◽

Trichoderma Sp ◽

Rate Of Reaction ◽

Higher Temperature ◽

Substrate Concentrations

Trichoderma spp. have been reported earlier for their excellent capacity of secreting extracellular α-galactosidase. This communication focuses on the optimization of culture conditions for optimal production of enzyme and its characterization. The evaluation of the effects of different enzyme assay parameters such as stability, pH, temperature, substrate concentrations, and incubation time on enzyme activity has been made. The most suitable buffer for enzyme assay was found to be citrate phosphate buffer (50 mM, pH 6.0) for optimal enzyme activity. This enzyme was fairly stable at higher temperature as it exhibited 72% activity at 60°C. The enzyme when incubated at room temperature up to two hours did not show any significant loss in activity. It followed Michaelis-Menten curve and showed direct relationship with varying substrate concentrations. Higher substrate concentration was not inhibitory to enzyme activity. The apparent Michaelis-Menten constant (Km), maximum rate of reaction (Vmax), Kcat, and catalytic efficiency values for this enzyme were calculated from the Lineweaver-Burk double reciprocal plot and were found to be 0.5 mM, 10 mM/s, 1.30 U mg−1, and 2.33 U mg−1 mM−1, respectively. This information would be helpful in understanding the biophysical and biochemical characteristics of extracellular α-galactosidase from other microbial sources.

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ANTAGONISTIC PROPERTIES OF THE LOCAL STRAIN TRICHODERMA SP 4

International Journal of Agriculture Environment and Bioresearch ◽

10.35410/ijaeb.2021.5690 ◽

2022 ◽

Vol 06 (06) ◽

pp. 152-166

Author(s):

H.Kh. Karimov ◽

B.I. Turaeva ◽

N.Sh. Azimova ◽

Kh.M. Khamidova

Keyword(s):

Local Strain ◽

Trichoderma Sp

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Development of Trichoderma sp. formulations in encapsulated granules (CG) and evaluation of conidia shelf-life

Biological Control ◽

10.1016/j.biocontrol.2017.08.020 ◽

2018 ◽

Vol 117 ◽

pp. 21-29 ◽

Cited By ~ 14

Author(s):

Gabriel Olivo Locatelli ◽

Gabriel Franco dos Santos ◽

Priscila S. Botelho ◽

Christine Lamenha Luna Finkler ◽

Luciano Avallone Bueno

Keyword(s):

Shelf Life ◽

Trichoderma Sp

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Evaluation of in vitro antifungal activity of potassium bicarbonate on Rhizoctonia solani AG 4 HG-I, Sclerotinia sclerotiorum and Trichoderma sp.

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb11.411 ◽

2011 ◽

Vol 10 (43) ◽

pp. 8605-8612 ◽

Cited By ~ 8

Author(s):

Erper Ismail ◽

Turkkan Muharrem ◽

H Karaca Guuml rsel ◽

Kılıc Guuml lay

Keyword(s):

Antifungal Activity ◽

Rhizoctonia Solani ◽

Sclerotinia Sclerotiorum ◽

Potassium Bicarbonate ◽

Trichoderma Sp ◽

In Vitro Antifungal Activity

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Efeito do lodo de esgoto na indução de supressividade in vitro a Phytophthora nicotianae

Fitopatologia Brasileira ◽

10.1590/s0100-41582003000100010 ◽

2003 ◽

Vol 28 (1) ◽

pp. 67-75 ◽

Cited By ~ 2

Author(s):

Carolina Leoni ◽

Raquel Ghini

Keyword(s):

Triticum Aestivum ◽

Medicago Sativa ◽

Phytophthora Nicotianae ◽

Phytophthora Spp ◽

Trichoderma Sp ◽

Aspergillus Sp

Uma alternativa de manejo das doenças causadas por Phytophthora spp. é o uso de matéria orgânica. No presente trabalho foi avaliada a potencialidade do lodo de esgoto na indução de supressividade in vitro a P. nicotianae. O efeito do lodo de esgoto incorporado ao solo na sobrevivência de P. nicotianae foi avaliado mediante um experimento fatorial com dois fatores: doses de lodo de esgoto (0, 10, 20 e 40% p/p) e concentrações de inóculo [0, 10 ou 20 g de grãos de trigo (Triticum aestivum) colonizados kg-1]. Aos 21 dias, quando aumentaram as doses de lodo de esgoto, a sobrevivência de P. nicotianae e os pHs das misturas diminuíram, e as condutividades elétricas (CE) aumentaram. As correlações entre a CE e a sobrevivência do patógeno foram negativas e significativas (P>0,05). Para estudar o efeito dos compostos químicos envolvidos na supressividade, foram obtidos extratos em água, H2SO4 2N e KOH 0,4N de misturas de areia – lodo de esgoto (20% p/p), e foram acrescentados ao meio de cultura e seu efeito avaliado no crescimento das colônias de P. nicotianae. O extrato ácido (H2SO4 2N) do tratamento com 20% de lodo de esgoto inibiu significativamente (P>0,05) o crescimento da colônia do patógeno. O efeito biológico foi estudado mediante isolamento de microrganismos em meio de cultura e seleção por antagonismo. No bioensaio com plântulas de alfafa (Medicago sativa) destacaram-se os isolados F9.1 (Aspergillus sp.) e A12.1 (actinomiceto, não identificado); e no teste de culturas pareadas destacou-se um Trichoderma sp. e dois actinomicetos por antibiose, e um Trichoderma sp. e três Aspergillus sp. por hiperparasitismo.

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