Magnolol Affects Cellular Proliferation, Polyamine Biosynthesis and  Catabolism-Linked Protein Expression and Associated Cellular Signaling  Pathways in Human Prostate Cancer Cells in vitro

Background: Prostate cancer is the most commonly diagnosed form of cancer in men in Canada and the United States. Both genetic and environmental factors contribute to the development and progression of many cancers, including prostate cancer.Context and purpose of this study: This study investigated the effects of magnolol, a compound found in the roots and bark of the magnolia tree Magnolia officinalis, on cellular proliferation and proliferation-linked activities of PC3 human prostate cancer cells in vitro.Results: PC3 cells exposed to magnolol at a concentration of 80 μM for 6 hours exhibited decreased protein expression of ornithine decarboxylase, a key regulator in polyamine biosynthesis, as well as affecting the expression of other proteins involved in polyamine biosynthesis and catabolism. Furthermore, protein expression of the R2 subunit of ribonucleotide reductase, a key regulatory protein associated with DNA synthesis, was significantly decreased. Finally, the MAPK (mitogen-activated protein kinase), PI3K (phosphatidylinositol 3-kinase), NFκB (nuclear factor of kappa-light-chain-enhancer of activated B cells) and AP-1 (activator protein 1) cellular signaling pathways were assayed to determine which, if any, of these pathways magnolol exposure would alter. Protein expressions of p-JNK-1 and c-jun were significantly increased while p-p38, JNK-1/2, PI3Kp85, p-PI3Kp85, p-Akt, NFκBp65, p-IκBα and IκBα protein expressions were significantly decreased.Conclusions: These alterations further support the anti-proliferative effects of magnolol on PC3 human prostate cancer cells in vitro and suggest that magnolol may have potential as a novel anti-prostate cancer agent.Key Words: prostate cancer cells, magnolol, polyamines, MAPK, PI3K, NFκB

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Magnolol affects the expression of polyamine biosynthesis and catabolism‐linked proteins in PC3 human prostate cancer cells in vitro (1011.1)

The FASEB Journal ◽

10.1096/fasebj.28.1_supplement.1011.1 ◽

2014 ◽

Vol 28 (S1) ◽

Author(s):

Brendan McKeown ◽

Robert Hurta

Keyword(s):

Prostate Cancer ◽

Cancer Cells ◽

Human Prostate ◽

Human Prostate Cancer ◽

Prostate Cancer Cells ◽

Polyamine Biosynthesis

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Proanthocyanidins from the American Cranberry (Vaccinium macrocarpon) Induce Cell Cycle Alterations in DU145 Human Prostate Cancer Cells in Vitro by Affecting the Expression of Cell Cycle-Associated Proteins

Functional Foods in Health and Disease ◽

10.31989/ffhd.v4i4.20 ◽

2014 ◽

Vol 4 (4) ◽

pp. 130

Author(s):

Joseph Kim ◽

Brendan McKeown ◽

Kunal Patel ◽

Adriana Catalli ◽

Marianna Kulka ◽

...

Keyword(s):

Prostate Cancer ◽

Cell Cycle ◽

Protein Expression ◽

Cancer Cells ◽

Vaccinium Macrocarpon ◽

Human Prostate ◽

Human Prostate Cancer ◽

Prostate Cancer Cells ◽

American Cranberry

Background: Prostate cancer is one of the most common cancers in the world. There are genetic and environmental factors that can potentially impact the development and progression of many types of cancer, including prostate cancer. As a consequence of environmental factors, such as diet having a potential effect on the development of prostate cancer, considerable interest in the possible health benefits associated with the inclusion and consumption of certain foods in the diet exists.Context and purpose of this study: This study describes the effects of a proanthocyanidin-enriched fraction (PACs) isolated from the American cranberry (Vaccinium macrocarpon) on the behaviour of androgen-refractory (insensitive) DU145 human prostate cancer cells in vitro.Results: Following treatment of DU145 human prostate cancer cells with 25 µg/mL of PACs for six hours, PACs significantly decreased the cellular viability of DU145 cells. PACs treatment (25 µg/mL for 6 hours) of DU145 cells increased the proportion of cells in the G2-M phase of the cell cycle and decreased the proportion of cells in the G1 phase of the cell cycle. These alterations were associated with changes in cell cycle regulatory proteins and other cell cycle associated proteins. PACs increased the expression of cyclin E, cyclin D1, CDK2 and CDK4, and decreased the expression of cyclin A and cyclin B1. The protein expression level of p27 increased, and the protein expression levels of p16INK4a, p21, and pRBp107 decreased in response to PACs treatment. The protein expression level of pRBp130 was unchanged in response to PACs treatment.Conclusions: These findings demonstrate that proanthocyanidins from the American cranberry can affect the behaviour of human prostate cancer cells in vitro and further support the potential health benefits associated with cranberries.Keywords: Prostate cancer, proanthocyanidin-enriched fraction (PACs), American cranberry (Vaccinium macrocarpon)

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Differential effects of transforming growth factor β on human prostate cancer cells in vitro

Molecular and Cellular Endocrinology ◽

10.1016/0303-7207(89)90115-9 ◽

1989 ◽

Vol 62 (1) ◽

pp. 79-87 ◽

Cited By ~ 111

Author(s):

George Wilding ◽

Gerhard Zugmeier ◽

Cornelius Knabbe ◽

Katherine Flanders ◽

Edward Gelmann

Keyword(s):

Prostate Cancer ◽

Growth Factor ◽

Cancer Cells ◽

Transforming Growth Factor ◽

Transforming Growth Factor Β ◽

Human Prostate ◽

Human Prostate Cancer ◽

Prostate Cancer Cells ◽

Differential Effects

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Blueberry flavonoids inhibit matrix metalloproteinase activity in DU145 human prostate cancer cells

Biochemistry and Cell Biology ◽

10.1139/o05-063 ◽

2005 ◽

Vol 83 (5) ◽

pp. 637-643 ◽

Cited By ~ 47

Author(s):

Michael D Matchett ◽

Shawna L MacKinnon ◽

Marva I Sweeney ◽

Katherine T Gottschall-Pass ◽

Robert A.R Hurta

Keyword(s):

Prostate Cancer ◽

Cancer Cells ◽

Apoptotic Cell ◽

Human Prostate ◽

Response To Treatment ◽

Human Prostate Cancer ◽

Prostate Cancer Cells ◽

The Matrix ◽

Ecm Degradation

Regulation of the matrix metalloproteinases (MMPs), the major mediators of extracellular matrix (ECM) degradation, is crucial to regulate ECM proteolysis, which is important in metastasis. This study examined the effects of 3 flavonoid-enriched fractions (a crude fraction, an anthocyanin-enriched fraction, and a proanthocyanidin-enriched fraction), which were prepared from lowbush blueberries (Vaccinium angustifolium), on MMP activity in DU145 human prostate cancer cells in vitro. Using gelatin gel electrophoresis, MMP activity was evaluated from cells after 24-hr exposure to blueberry fractions. All fractions elicited an ability to decrease the activity of MMP-2 and MMP-9. Of the fractions tested, the proanthocyanidin-enriched fraction was found to be the most effective at inhibiting MMP activity in these cells. No induction of either necrotic or apoptotic cell death was noted in these cells in response to treatment with the blueberry fractions. These findings indicate that flavonoids from blueberry possess the ability to effectively decrease MMP activity, which may decrease overall ECM degradation. This ability may be important in controlling tumor metastasis formation.Key words: blueberry flavonoids, MMP activity, prostate cancer cells.

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