scholarly journals Neonatal Diarrhea, AE

2020 ◽  
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2020 ◽  
Vol 41 (2) ◽  
pp. 112-115
Author(s):  
L Dethioux ◽  
S Jasienski ◽  
P Van Laer ◽  
M Maka
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Author(s):  
Miguel L. O’Ryan ◽  
James P. Nataro ◽  
Thomas G. Cleary
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2020 ◽  
Vol 7 (2) ◽  
pp. 48
Author(s):  
Anna Vidal ◽  
Laia Aguirre ◽  
Chiara Seminati ◽  
Montse Tello ◽  
Noelia Redondo ◽  
...  

Escherichia coli is considered one of the most common agents associated with neonatal diarrhea in piglets. The aim of this work was to characterize the pathogenic and antimicrobial resistance (AMR) profiles of 122 E. coli strains isolated from pigs suffering diarrhea (n = 94) and pigs without diarrhea (n = 28) of 24 farms in Spain. Virulence factors, toxins and AMR (ESBL and colistin) genes and AMR phenotypes of E. coli isolates were analyzed. Low prevalence of pathogenic E. coli strains (26%) was found in both groups. However, ETEC and VTEC strains were more frequently isolated from diarrheic piglets. Irrespectively of diarrhea occurrence, 97.5% of the strains showed a multidrug-resistance (MDR) profile to aminopenicillins, sulfonamides and tetracyclines. It was found that 22% of E. coli was CTX-M+, with CTX-M-14 being the principal allelic variant. Remarkably, 81.5% of CTX-M+ strains were isolated from diarrheic animals and presented an extended MDR profile to aminopenicillins, quinolones and aminoglycosides. Finally, low frequencies of colistin resistance genes mcr-1 (4/122) and mcr-4 (1/122) were found. MDR E. coli strains are circulating in pig farms of Spain, representing a serious threat to animal and public health. More appropriate diagnostic approaches (genetic and AMR phenotypic analysis) should be implemented in animal health to optimize antibiotic treatments.



2011 ◽  
Vol 31 (6) ◽  
pp. 505-510 ◽  
Author(s):  
Ricardo T. Lippke ◽  
Sandra M. Borowski ◽  
Sandra M.T. Marques ◽  
Suelen O. Paesi ◽  
Laura L. Almeida ◽  
...  

A case-control study was carried out in litters of 1 to 7-day-old piglets to identify the main infectious agents involved with neonatal diarrhea in pigs. Fecal samples (n=276) from piglets were collected on pig farms in the State of Rio Grande do Sul, Brazil, from May to September 2007. Litters with diarrhea were considered cases (n=129) and normal litters (n=147) controls. The samples were examined by latex agglutination test, PAGE, conventional isolating techniques, ELISA, PCR, and microscopic methods in order to detect rotavirus, bacterial pathogens (Escherichia coli, Clostridium perfringens type A and C, and Clostridium difficile), and parasites (Coccidian and Cryptosporidium spp.). Outbreaks of diarrhea were not observed during sampling. At least one agent was detected in fecal samples on 25 out of 28 farms (89.3%) and in 16 farms (57.1%) more than one agent was found. The main agents diagnosed were Coccidia (42.86%) and rotavirus (39.29%). The main agents identified in litters with diarrhea were Clostridium difficile (10.6%), Clostridium perfringens type A (8.8%) and rotavirus (7.5%); in control litters, Clostridium difficile (16.6%) and Coccidian (8.5%). Beta hemolytic Escherichia coli and Clostridium perfringens type C were not detected. When compared with controls, no agent was significantly associated with diarrhea in case litters. These findings stress the need for caution in the interpretation of laboratorial diagnosis of mild diarrhea in neonatal pigs, as the sole detection of an agent does not necessarily indicate that it is the cause of the problem.



2010 ◽  
Vol 47 (4) ◽  
pp. 751-757 ◽  
Author(s):  
D. G. Sledge ◽  
P. K. Danieu ◽  
C. A. Bolin ◽  
S. R. Bolin ◽  
A. Lim ◽  
...  
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1989 ◽  
Vol 1 (1) ◽  
pp. 74-75 ◽  
Author(s):  
Stanley Allen ◽  
Jeff Mitchell ◽  
Wendy Jones ◽  
Marie Quinn


2004 ◽  
Vol 41 (4) ◽  
pp. 433-437 ◽  
Author(s):  
Y. Wada ◽  
M. Kato ◽  
S. Yamamoto ◽  
T. Shibahara ◽  
Y. Ishikawa ◽  
...  


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