scholarly journals Free Insulin Measurement

2020 ◽  
Author(s):  
Keyword(s):  
1992 ◽  
Vol 67 (01) ◽  
pp. 117-120 ◽  
Author(s):  
Helena Vuorinen-Markkola ◽  
llpo Puhakainen ◽  
Hannele Yki-Järvinen

SummaryIn crossectional studies a positive correlation has been found between circulating insulin, triglycerides and plasminogen activator inhibitor (PAI-1) activity. To directly examine the effect of insulin on PAI-1 activity in vivo, we determined the response of PAI-1 activity in 17 normal subjects to acute hyperinsulinemia (serum free insulin 92 ± 8 mU/l) during maintenance of normoglycemia (plasma glucose 5.1 ± 0.1 mmol/l). In 12 matched control subjects PAI-1 activity was measured during infusion of saline (serum free insulin 3.6 ± 0.3 mU/l, plasma glucose 5.2 ± 0.1 mmol/l). Plasma PAI-1 activity decreased during the insulin infusion from 9.0 α 1.4 to 5.6 α 0.8 U/ml (p <0.01), and during saline infusion from 7.0 ± 1.4 to 4.3 ± 0.6 U/ml (p <0.05). Serum triglyceride concentrations decreased from 1.09 ± 0.20 to 0.76 ± 0.09 mmol/l (p < 0.001) during hyperinsulinemia but remained unchanged during the saline infusion (1.04 ± 0.11 vs. 1.02 ± 0.12 mmol/l, NS). We conclude that insulin does not acutely change plasma PAI-1 activity, and that acute insulin-induced changes in serum triglycerides occur independently from those of PAI-1 activity.


2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Jae Geun Song ◽  
Sang Hoon Lee ◽  
Hyo-Kyung Han

Abstract Background There is a strong need for non-invasive and patient-friendly delivery systems of protein drugs for long-term therapy. However, oral delivery of protein drugs is a big challenge due to many barriers including instability in the gastrointestinal (GI) tract and low permeability. To overcome the absorption barriers in GI tract and improve the patient compliance, this study aimed to develop an M cell targeted-nanocomposite delivery system of protein drugs. Results An aminoclay-protein core complex (AC-Ins) was prepared by using insulin as a model protein and then sequentially coated with Ulex europaeus agglutinin 1 (UEA-1) for M-cell targeting and the pH sensitive polymer, Eudragit® L100 (EUAC-Ins). All nanoparticles were obtained with a high entrapment efficiency (> 90%) and their structural characteristics were confirmed by Fourier transform-infrared spectroscopy, energy dispersive X-ray spectroscopy, and circular dichroism. Among the developed nanoparticles, EUAC-Ins effectively suppressed drug release at pH 1.2, while rapidly released drugs at pH 6.8 due to dissolution of the outer coating layer. The conformational stability of insulin entrapped in EUAC-Ins was well maintained in the presence of proteolytic enzymes. Compared to free insulin, EUAC-Ins increased the membrane transport of insulin by 4.4-fold in M cells. In parallel, oral administration of EUAC-Ins in mice enhanced insulin uptake by 4.1-fold in the intestinal Peyer’s patches and 2.6-fold in intestinal epithelium tissues with normal villi, compared to free insulin. Orally administered EUAC-Ins decreased significantly the blood glucose level in diabetic mice, while the effect of oral insulin solution was negligible. Conclusion An M cell targeted-ternary nanocomposite system obtained by dual coating of the aminoclay-protein core complex with UEA-1 and a pH dependent polymer is promising as an effective oral protein delivery carrier.


Diabetes ◽  
1954 ◽  
Vol 3 (6) ◽  
pp. 466-475
Author(s):  
G. E. Anderson ◽  
M. Fribourg

2021 ◽  
Vol 160 (6) ◽  
pp. S-305-S-306
Author(s):  
Kyu Shik Mun ◽  
Jaimie D. Nathan ◽  
Maisam Abu-El-Haija ◽  
Tom K. Lin ◽  
Deborah A. Elder ◽  
...  

2021 ◽  
pp. 193229682199152
Author(s):  
Jana Winkelkötter ◽  
Thore Reitz

Background: The use of tube-free insulin pumps is increasing. To protect the environment, the use of resources and the amount of emissions into the environment should be kept as low as possible when designing these systems. In addition to basic waste avoidance, the composition of the waste produced must be considered. Methods: To compare current tube-free pumps from an ecological standpoint, a tube-free insulin pump with a modular design and two non-modular tube-free pumps were subjected to manual separation, manual sorting, characterization, and mass determination. The annual waste volume of a user was measured, and the recyclability was assessed. The global warming potential (GWP) resulting from extraction of raw materials, energetic utilization of waste, and landfill of the incineration residues was balanced. Results: For the modular tube-free pump, a total waste volume of 5.5 kg/a (recycling percentage 44.3%) was determined. The non-modular systems generated 4.9 kg/a (recycling percentage 14.6%) and 5.1 kg/a (recycling percentage 16.0%) waste. The product-specific GWP of the modular system was approximately 50% lower than that of the non-modular systems; the packaging-specific GWP was 2.5 times higher. In total, a GWP of 13.6 kg CO2-equivalent per year could be determined for the modular system and a GWP of 15.5 kg CO2-equivalent per year for the non-modular systems. Conclusions: Although the modular micropump has a higher total waste volume, a greater ecological potential can be attributed to it. This is based on the recyclability of the system due to its modularity and the possible reduction of packaging waste.


2007 ◽  
Vol 9 (s1) ◽  
pp. S-57-S-64 ◽  
Author(s):  
Per Wollmer ◽  
Thomas R. Pieber ◽  
Mari-Anne Gall ◽  
Stephen Brunton

Diabetes Care ◽  
1979 ◽  
Vol 2 (6) ◽  
pp. 457-464 ◽  
Author(s):  
M. I. Schmidt ◽  
A. Hadji-Georgopoulos ◽  
M. Rendell ◽  
S. MargoliS ◽  
D. Kowarski ◽  
...  
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