scholarly journals THE EFFECT OF INTRAVENOUS STEM CELLS INJECTION ON THE INDICATORS OF THE ANTIOXIDANT SYSTEM OF KIDNEYS OF RATS AFTER APPLICATION OF A BONE DEFECT

2021 ◽  
Vol 7 (1) ◽  
pp. 24-31
Author(s):  
A.N. Serkina
Keyword(s):  
Stem Cells ◽  
Bone Defect ◽  
Antioxidant System

Repair of bone defect with vascularized tissue engineered bone graft seeded with mesenchymal stem cells in rabbits

Microsurgery ◽  
2011 ◽  
Vol 31 (2) ◽  
pp. 130-137 ◽  
Author(s):  
Mingdong Zhao ◽  
Jian Zhou ◽  
Xilei Li ◽  
Taolin Fang ◽  
Wenda Dai ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Graft ◽  
Bone Defect

scholarly journals SHED Aggregate Derived Exosomes-shuttled miR-222 Promotes the Regenerative Properties of Periodontal Ligament Stem Cells

2021 ◽  
Author(s):  
Feng Zhou ◽  
Jia Guo ◽  
Fang Wang ◽  
Wanmin Zhao ◽  
Xiaoning He ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Defect ◽  
Periodontal Ligament ◽  
Underlying Mechanism ◽  
Tube Formation ◽  
Deciduous Teeth ◽  
Aggregate Formation ◽  
Periodontal Ligament Stem Cells ◽  
Mirna Sequencing

Abstract Background: Periodontal ligament stem cells (PDLSCs) aggregate is still limited in clinical application for lack of angiogenesis. This study aimed to investigate the effects and underlying mechanism of exosomes derived from stem cells from human exfoliated deciduous teeth (SHED) aggregate (SA-Exo) on the aggregate formation and angiogenic properties of PDLSCs.Methods: SA-Exo were isolated by ultracentrifugation. The effect of SA-Exo on the aggregate formation and angiogenic differentiation of PDLSCs were evaluated by investigating extracellular matrix (ECM) deposition and tube formation assay. MicroRNA (miRNA) sequencing was employed to screen different miRNA expression. The effect of targeting miRNA on ECM deposition and angiogenesis of PDLSCs aggregate was investigated after overexpression and inhibition of miRNA. Periodontal bone defect rat models were established to evaluate the effect of the PDLSCs aggregate and SA-Exo combination on periodontal bone regeneration. Results: SA-Exo could significantly enhance the ECM deposition and angiogenic ability of PDLSCs. The expression of ECM-associated proteins (COL-I, integrinβ1, and fibronectin), angiogenesis-related proteins (PDGF, ANG, TGFβRII), and related pathway (p-SMAD1/5 and p-SMAD2/3) were upregulated in PDLSCs aggregate with SA-Exo. Mechanistically, miR-222 was found relatively abundant in SA-Exo, which promoted ECM deposition and angiogenesis of PDLSCs. In vivo experiment further validated that combinational use of PDLSCs aggregate and SA-Exo promote more bone formation and neovascularization in rat’s periodontal bone defect.Conclusions: SA-Exo-shuttled miR-222 contributes to PDLSCs aggregate engineering by promoting aggregate formation and angiogenesis, which might through activate the TGF-β/SMAD signaling pathway.

Repair of Calvarial Bone Defect Using Jarid1a-Knockdown Bone Mesenchymal Stem Cells in Rats

2018 ◽  
Vol 24 (9-10) ◽  
pp. 711-718 ◽  
Author(s):  
Yuan Deng ◽  
Tao Guo ◽  
Jipeng Li ◽  
Li Guo ◽  
Ping Gu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Defect ◽  
Calvarial Bone ◽  
Bone Mesenchymal Stem Cells ◽  
Calvarial Bone Defect

CASE REPORT: THREE CASES OF NON-UNION FRACTURE IN DOGS TREATED WITH ADIPOSE-DERIVED ADULT STEM CELLS COMBINED WITH AUTOLOGOUS CANCELLOUS BONE AND SYNTHETIC BONE SUBSTITUTES

2017 ◽  
Vol 43 (01) ◽  
pp. 1-9
Author(s):  
Kun-Lung Tsai
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Therapy ◽  
Bone Defect ◽  
Stem Cell Therapy ◽  
Bone Plates ◽  
Intact Male ◽  
Non Union ◽  
Post Surgery ◽  
Veterinary Hospital

The first case was a 4-year old, 10[Formula: see text]kgs intact male Shiba Inu presenting fracture of the distal third of its right ulna and radius. The patient was first managed at a Local Veterinary Hospital (LVH) twice with bone plates but non-union was observed, along with bone infection and bone defect on a large area. The fracture site was re-stabilized and given stem cell therapy at Famous Veterinary Hospital (FVH). The area of bone defect reduced significantly with prominent callus formation. The second case was a 9-year old, 3.3[Formula: see text]kgs intact female Toy Poodle with fracture of the distal right radius and ulna. The fracture was first stabilized with bone plates at LVH but non-union was observed. Resorption of bone was visible radiographically around the proximal screw, which led to fracture and implant loosening at that location. Pan-arthrodesis followed after stem cells administration was performed at FVH. Radiography showed sufficient growth of the radius and union of the ulna occurred at 145 days after therapy. The third case was a 3.5-year old, 10[Formula: see text]kgs intact male Mongrel attended at LVH for a humerus fracture. Fracture reduction with bone plates was attempted twice at LVH but both loosened eventually. At FVH, an external skeletal fixator was used to stabilize the fracture, followed by stem cell therapy. There was radiographic evidence that the humerus healed well by the 35th day after therapy and was completely united by Day 113 of post-surgery and therapy.

Transplantation of Dental Pulp Stem Cells in Experimental Bone Defect

2017 ◽  
Vol 34 ◽  
pp. 94-100 ◽  
Author(s):  
Endang W. Bachtiar ◽  
Fatma S. Dewi ◽  
Ahmad Aulia Yusuf ◽  
Rahmi Ulfiana
Keyword(s):  
Stem Cells ◽  
Animal Model ◽  
Bone Regeneration ◽  
Bone Tissue ◽  
Bone Defect ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Bone Tissue Regeneration ◽  
Histological Evaluation ◽  
Control Rabbit

This is preliminary study in order to investigate the effect of dental pulp stem cells (DPSCs) on bone regeneration in an animal model. New Zealand rabbits were used as animal model. The critical defect was created in femoral bone and transplantation of DPSCs applied into bone defect. A colorimetric assay was used to detect ALP level in rabbit’s serum. Bone tissue regeneration was evaluated by histological analysis. In the 2nd week, the treated rabbit show increasing in the activity of ALP (157,925 μU) compared to control rabbit (155,361 μU). This increasing trend continues significantly in DPSCs rabbit (169.750 μU) compared to control rabbit (160.406) after 4 weeks. Histological evaluation revealed that the amount of bone lamellae and osteocytes were filled the defect area of DPSCs treated rabbit. Conclusions: Transplantation of DPSCs accelerating bone regeneration by raising ALP level and forming new bone tissue.

Clinical and Radiographic Effects of Bone Marrow Derived Mesenchymal Stem Cells Along with Local Insulin Therapy in Healing of Segmental Bone Defect in Diabetic Rabbits

2019 ◽  
Author(s):  
Sonu Jaiswal ◽  
Narendra Singh Jadon ◽  
Deepti Bodh ◽  
R K Vishwakarma
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Insulin Therapy ◽  
Bone Defect ◽  
Therapy Group ◽  
Allogenic Bone ◽  
Group A ◽  
Hydroxyapatite Scaffold ◽  
Diabetic Rabbits

The objective of present study was to investigate the efficacy of cultured allogenic bone marrow derived mesenchymal stem cell (BMMSCs) implant with insulin therapy for increasing osteosynthesis in bone gap defects in diabetic rabbits. Thirty six, clinically healthy New Zealand White rabbits were divided into four groups: A, B, C and D. Diabetes was induced experimentally in all rabbits, except group A. A 5 mm bone defect was created in right radii of all rabbits and following treatment was given: hydroxyapatite granules (group A and B), hydroxyapatite granules and insulin therapy (group C), cultured allogenic BMMSCs in hydroxyapatite scaffold and insulin therapy (group D). Clinical and radiographic parameters were recorded at days 0, 30, 60 and 90. It may be conducted that cultured allogenic bone marrow derived mesenchymal stem cells in hydroxyapatite scaffold along with local insulin therapy produces faster and better healing in alloxan-induced diabetic rabbits compared to healthy control, untreated diabetic and local insulin treated groups.

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