scholarly journals Study on growth and lipid biosynthesis of microalgae strains isolated in Vietnam

2014 ◽  
Vol 17 (2) ◽  
pp. 15-26
Author(s):  
Lan Thi My Nguyen ◽  
Tham Thi Mong Doan ◽  
Hung Hiep Huynh ◽  
Loan Thi Thanh Le ◽  
Ho Thanh Pham ◽  
...  
Keyword(s):  
Lipid Production ◽  
Screening Method ◽  
Nile Red ◽  
Biodiesel Production ◽  
Rapid Screening ◽  
High Yield ◽  
Lipid Contents ◽  
Yield Production ◽  
Microalgal Biodiesel ◽  
Local Species

Microalgal biodiesel is considered an alternative to fossil fuel and also potentially reduce the introduction of new CO2 by displacing fossil hydrocarbon fuels. Nowadays, researching on renewable energy is mainly focus on biodiesel from microalgae due to their fast growth rates and high-yield production. In order to improve this field in Vietnam, we isolated some local species and used rapid screening method for lipid production in microalgae based on Nile Red fluorescence. Futhermore, providing a reference for the future biodiesel production using these microalgal species, we also determined lipid contents of these species ranged from 8.89% to 29.12% in natural cuturing conditions by soxhlet and Bligh & Dyer methods.

scholarly journals Biochemical Modulation of Lipid Pathway in MicroalgaeDunaliellasp. for Biodiesel Production

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Ahmad Farhad Talebi ◽  
Masoud Tohidfar ◽  
Seyedeh Mahsa Mousavi Derazmahalleh ◽  
Alawi Sulaiman ◽  
Azhari Samsu Baharuddin ◽  
...  
Keyword(s):  
Feedback Inhibition ◽  
Lipid Production ◽  
Cetane Number ◽  
Nile Red ◽  
Transcript Abundance ◽  
Point Of View ◽  
Biodiesel Production ◽  
Biochemical Modulation ◽  
Oil Accumulation ◽  
Microalgal Biodiesel

Exploitation of renewable sources of energy such as algal biodiesel could turn energy supplies problem around. Studies on a locally isolated strain ofDunaliellasp. showed that the mean lipid content in cultures enriched by 200 mg L−1myoinositol was raised by around 33% (1.5 times higher than the control). Similarly, higher lipid productivity values were achieved in cultures treated by 100 and 200 mg L−1myoinositol. Fluorometry analyses (microplate fluorescence and flow cytometry) revealed increased oil accumulation in the Nile red-stained algal samples. Moreover, it was predicted that biodiesel produced from myoinositol-treated cells possessed improved oxidative stability, cetane number, and cloud point values. From the genomic point of view, real-time analyses revealed that myoinositol negatively influenced transcript abundance ofAccDgene (one of the key genes involved in lipid production pathway) due to feedback inhibition and that its positive effect must have been exerted through other genes. The findings of the current research are not to interprete that myoinositol supplementation could answer all the challenges faced in microalgal biodiesel production but instead to show that “there is a there there” for biochemical modulation strategies, which we achieved, increased algal oil quantity and enhanced resultant biodiesel quality.

scholarly journals Harnessing pongamia shell hydrolysate for triacylglycerol agglomeration by novel oleaginous yeast Rhodotorula pacifica INDKK

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Kukkala Kiran Kumar ◽  
Farha Deeba ◽  
Sauraj ◽  
Yuvraj Singh Negi ◽  
Naseem A. Gaur
Keyword(s):  
High Throughput Screening ◽  
Oleaginous Yeast ◽  
Lipid Production ◽  
Cetane Number ◽  
Nile Red ◽  
Major Fatty Acid ◽  
Biodiesel Production ◽  
Microbial Lipid ◽  
Dry Cell Weight ◽  
Pre Treatment

Abstract Background To meet the present transportation demands and solve food versus fuel issue, microbial lipid-derived biofuels are gaining attention worldwide. This study is focussed on high-throughput screening of oleaginous yeast by microwave-aided Nile red spectrofluorimetry and exploring pongamia shell hydrolysate (PSH) as a feedstock for lipid production using novel oleaginous yeast Rhodotorula pacifica INDKK. Results A new oleaginous yeast R. pacifica INDKK was identified and selected for microbial lipid production. R. pacifica INDKK produced maximum 12.8 ± 0.66 g/L of dry cell weight and 6.78 ± 0.4 g/L of lipid titre after 120 h of growth, showed high tolerance to pre-treatment-derived inhibitors such as 5-hydroxymethyl furfural (5-HMF), (2 g/L), furfural (0.5 g/L) and acetic acid (0.5 g/L), and ability to assimilate C3, C5 and C6 sugars. Interestingly, R. pacifica INDKK showed higher lipid accumulation when grown in alkali-treated saccharified PSH (AS-PSH) (0.058 ± 0.006 g/L/h) as compared to acid-treated detoxified PSH (AD-PSH) (0.037 ± 0.006 g/L/h) and YNB medium (0.055 ± 0.003 g/L/h). The major fatty acid constituents are oleic, palmitic, linoleic and linolenic acids with an estimated cetane number (CN) of about 56.7, indicating the good quality of fuel. Conclusion These results suggested that PSH and R. pacifica INDKK could be considered as potential feedstock for sustainable biodiesel production.

scholarly journals Review: Managing sheep and goats for sustainable high yield production

animal ◽  
2021 ◽  
pp. 100293
Author(s):  
J. Simões ◽  
J.A. Abecia ◽  
A. Cannas ◽  
J.A. Delgadillo ◽  
D. Lacasta ◽  
...  
Keyword(s):  
High Yield ◽  
Sheep And Goats ◽  
Yield Production ◽  
High Yield Production

scholarly journals High‐Yield Production, Characterization, and Functionalization of Recombinant Magnetosomes in the Synthetic Bacterium Rhodospirillum rubrum “magneticum”

2021 ◽  
pp. 2101017
Author(s):  
Frank Mickoleit ◽  
Sabine Rosenfeldt ◽  
Mauricio Toro‐Nahuelpan ◽  
Miroslava Schaffer ◽  
Anna S. Schenk ◽  
...  
Keyword(s):  
Rhodospirillum Rubrum ◽  
High Yield ◽  
Yield Production ◽  
High Yield Production

scholarly journals Development of UHPLC/Q-TOF Analysis Method to Screen Glycerin for Direct Detection of Process Contaminants 3-Monochloropropane-1,2-diol Esters (3-MCPDEs) and Glycidyl Esters (GEs)

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2449
Author(s):  
Lauren Girard ◽  
Kithsiri Herath ◽  
Hernando Escobar ◽  
Renate Reimschuessel ◽  
Olgica Ceric ◽  
...  
Keyword(s):  
Renal Failure ◽  
Solid Phase ◽  
Direct Detection ◽  
Screening Method ◽  
Extraction Procedure ◽  
Biodiesel Production ◽  
Food Contaminants ◽  
Exact Mass ◽  
Glycidyl Esters

The U.S. Food and Drug Administration’s (FDA′s) Center for Veterinary Medicine (CVM) has been investigating reports of pets becoming ill after consuming jerky pet treats since 2007. Renal failure accounted for 30% of reported cases. Jerky pet treats contain glycerin, which can be made from vegetable oil or as a byproduct of biodiesel production. Glycidyl esters (GEs) and 3-monochloropropanediol esters (3-MCPDEs) are food contaminants that can form in glycerin during the refining process. 3-MCPDEs and GEs pose food safety concerns, as they can release free 3-MCPD and glycidol in vivo. Evidence from studies in animals shows that 3-MCPDEs are potential toxins with kidneys as their main target. As renal failure accounted for 30% of reported pet illnesses after the consumption of jerky pet treats containing glycerin, there is a need to develop a screening method to detect 3-MCPDEs and GEs in glycerin. We describe the development of an ultra-high-pressure liquid chromatography/quadrupole time-of-flight (UHPLC/Q-TOF) method for screening glycerin for MCPDEs and GEs. Glycerin was extracted and directly analyzed without a solid-phase extraction procedure. An exact mass database, developed in-house, of MCPDEs and GEs formed with common fatty acids was used in the screening.

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