METHOD FOR MEASURING THE MASS FRACTION OF THE ACTIVE INGREDIENT OF HEXYTHIAZOX IN LIQUID HOMOGENEOUS FORMULATION BY HIGH EFFECTIVE LIQUID CHROMATOGRAPHY

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Azizbek Bektemirov ◽  
Farkhod Hoshimov ◽  
Oybek Ergashev
Keyword(s):  
Liquid Chromatography ◽  
Active Ingredient ◽  
Mass Fraction

Development and Validation of A Reversed Phase-High Performance Liquid Chromatography Method for the Quantitative Estimation of Ramipril Drug Content from Formulated Dosage Form

2016 ◽  
Vol 6 (3) ◽  
Author(s):  
Raju Chandra ◽  
Manisha Pant ◽  
Harchan Singh ◽  
Deepak Kumar ◽  
Ashwani Sanghi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reversed Phase ◽  
Uv Detector ◽  
Chromatography Method ◽  
Drug Content

A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of Remipril drug content from marketed bulk tablets. The active ingredient of Remipril separation achieved with C18 column using the methanol water mobile phase in the ratio of 40:60 (v/v). The active ingredient of the drug content quantify with UV detector at 215 nm. The retention time of Remipril is 5.63 min. A good linearity relation (R2=0.999) was obtained between drug concentration and average peak areas. The limit of detection and limit of quantification of the instrument were calculated 0.03 and 0.09 µg/mL, respectively. The accuracy of the method validation was determined 102.72% by recoveries method.

Determination of total and A1-Type β-casein in milk and milk-derivedingredients by liquid chromatography – mass spectrometry using characteristic tryptic peptides

2020 ◽  
Author(s):  
Stefan Ehling ◽  
Meibo Wang ◽  
Luke Weber
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Mass Fraction ◽  
Health Claims ◽  
Whey Protein Concentrate ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Limit Of Quantitation ◽  
Dry Milk ◽  
Nonfat Dry Milk

Abstract Background Gastrointestinal digestion of A1-type β-casein is conducive to β-casomorphin-7 with potential adverse digestive health effects. Monitoring of A1-type β-casein concentration in milk and milk-derived ingredients used in the formulation of A2-type nutritional products with associated health claims is important from a quality standpoint. Objective New analytical methods were developed and validated for total and A1-type β-casein in milk and milk-derived ingredients. Data on total and A1-type β-casein concentrations in milk, nonfat dry milk, and whey protein concentrate was generated. Methods The methods are based on a bottom-up proteomic approach using tryptic marker peptides and stable isotope dilution liquid chromatography – mass spectrometry. The measurement includes all protein sequences (intact, modified, partial) which are potential sources of β-casomorphin-7. Results Total β-casein was quantified using a neat calibration curve. Recovery and between-day precision RSD were 98% and 5.8%, respectively. A1-type β-casein was quantified by the method of standard additions. Between-day precision RSD was 7.2% and limit of quantitation was 0.01% in nonfat dry milk. The mass fraction of A1-type β-casein in β-casein standard was 0.444. Samples manufactured from A2-type milk contained 0.26–5.0% A1-type β-casein relative to total β-casein. Conclusions The methods described enable the monitoring of the A1-type β-casein concentration in milk and milk-derived ingredients destined for the manufacture of A2-type products with associated health claims. Highlights New methods are presented for the analysis of total and A1-type β-casein in milk and milk-derived ingredients. Mass fraction of A1-type β-casein in commercial β-casein standard was determined to enable its use as calibrant.

ACTIVITY ON THE BETA-EXOTOXIN OF BACILLUS THURINGIENSIS VAR. THURINGIENSIS IN THE ESCHERICHIA COLI DNA REPAIR ASSAY FOR BACTERIAL GENOTOXICITY1,2

1985 ◽  
Vol 20 (1) ◽  
pp. 104-108 ◽  
Author(s):  
George E. Cantwell ◽  
Robert J. Argauer ◽  
William W. Cantelo
Keyword(s):  
Escherichia Coli ◽  
Dna Repair ◽  
Liquid Chromatography ◽  
Bacillus Thuringiensis ◽  
Active Ingredient ◽  
Colorado Potato Beetle ◽  
Potato Beetle ◽  
Experimental Preparation

The beta-exotoxin of an experimental preparation of Bacillus thuringiensis var. thuringiensis was bioassayed against neonate Colorado potato beetle larvae and its lethal concentrations determined. The amount of active ingredient in the preparation was determined by liquid chromatography. The Escherichia coli DNA Repair Assay was used to determine the DNA damaging potential of the beta-exotoxin, which was found to be negative in this system.

scholarly journals Photostability of Loratadine Inclusion Complexes with Natural Cyclodextrins

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Patricia Elizabeth Rivas-Granizo ◽  
Leandro Giorgetti ◽  
Humberto Gomes Ferraz
Keyword(s):  
Thermal Analysis ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Statistical Analysis ◽  
Inclusion Complexes ◽  
Active Ingredient ◽  
High Performance ◽  
Full Recovery ◽  
Stoichiometric Ratios ◽  
Histamine Antagonist

The purpose of this study was to evaluate the photostability of inclusion complexes of the histamine antagonist loratadine (LORA) withα-,β-, andγ-cyclodextrins (CDs). Accordingly, binary drug-CD complexes were prepared using the coevaporation method at 1 : 1, 1 : 2, and 1 : 3 stoichiometric ratios, which were characterized by thermal analysis. Subsequently, solutions of the complexes at 500 μg mL−1in HCl 0.1 M were subjected to irradiation in a photostability chamber for 12 hours, and the content of the remaining active ingredient was quantified by means of high-performance liquid chromatography. It is possible to observe the presence of two products originating from photodegradation (P1 and P2), which were identified in solutions of loratadine withα- andβ-CD. By means of statistical analysis, we conclude that the drug:α-CD and drug:γ-CD (1 : 1) complexes proved to be more efficient in the photostability assay, obtaining a nonsignificant level of degradation and full recovery of LORA.

QUALITY ASSESSMENT AND QUANTIFICATION OF GENISTEIN IN DIETARY SUPPLEMENTS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY, QUANTITATIVE NUCLEAR MAGNETIC RESONANCE, AND TWO-DIMENSIONAL DIFFUSION ORDERED SPECTROSCOPY 1H

2020 ◽  
pp. 132-142
Author(s):  
VLACHOU IOANNA ◽  
PAPASOTIRIOU IOANNIS
Keyword(s):  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
Dietary Supplements ◽  
Active Ingredient ◽  
High Performance ◽  
Two Dimensional ◽  
Quantitative Nuclear Magnetic Resonance ◽  
Dosy Nmr

Objective: The aim of the present study is to analyze four herbal dietary supplements containing genistein by liquid chromatography, quantitative nuclear magnetic resonance (qNMR), and diffusion ordered spectroscopy (DOSY) 1H NMR. Methods: Quantification of the active ingredient, genistein, is carried out by high-performance liquid chromatography (HPLC) and qNMR. Two-dimensional (2D) DOSY NMR also allows the qualitative analysis of the samples with the detection of active ingredient and excipients present in the formulations. Results: The validated HPLC and qNMR methods showed that all four supplements contain genistein in different amounts, and 2D DOSY NMR provides a clear image of all ingredients in the formulations. Conclusion: The use of the three techniques provides detailed information on each product and its contents, and all of them are currently used for the quality control of natural supplements by our laboratory.

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