Biochemistry of postharvest spoilage of sweet potato (Ipomoea batatas L.). 2. Comparison of cellulolytic enzyme production in cultures and fungi-infected sweet potato tubers

Annals of Tropical Research ◽

10.32945/atr2814.2006 ◽

2006 ◽

pp. 48-57

Author(s):

R. C. Ray

Keyword(s):

Sweet Potato ◽

Enzyme Production ◽

Ipomoea Batatas ◽

Rhizopus Oryzae ◽

Cellulose Synthesis ◽

Cellulolytic Enzyme ◽

Optimum Temperature ◽

Potato Tubers

The study was conducted to determine the production in vitro and in vivo of cellulases by Botrydiplodia theobromae and Rhizopus oryzae. Isolates of these organisms were obtained from the postharvest decay of sweetpotato tubers. Results revealed that B. theobrornae and R. oryzae which were isolated from postharvest spoilage of sweetpotato tubers produced endo-13-1,4-glucanase and exo-V-1 ,4-glucanase in culture and in fungi-infected tissues of sweetpotato tubers. The optimum temperature and pH for cellulose synthesis and activity were 30°C and pH 6.5, respectively.

Download Full-text

Mechanisms of pathogenesis in Sclerotium bataticola on sunflowers. II. Pectolytic and cellulolytic enzyme production in vitro and in vivo

Canadian Journal of Botany ◽

10.1139/b70-155 ◽

1970 ◽

Vol 48 (6) ◽

pp. 1073-1077 ◽

Cited By ~ 8

Author(s):

Yu-Ho Chan ◽

W. E. Sackston

Keyword(s):

Enzyme Production ◽

Pectin Methylesterase ◽

Cellulolytic Enzyme ◽

Culture Filtrates ◽

Healthy Control ◽

Host Tissues

Pectin methylesterase (PME), endopolygalacturonase (Endo-PG), exopolygalacturonase (Exo-PG), pectin trans-eliminase (PTE), polygalacturonase trans-eliminase (PGTE), cellulase, and cellobiase activities were investigated in culture filtrates of Sclerotium bataticola, and in extracts of inoculated and uninoculated sunflower stems. All of the enzymes except PTE were produced in culture filtrates of the pathogen and in diseased host tissues. Only PME was detected in healthy control plants.

Download Full-text

Bioactive Compounds, Antioxidants, and Health Benefits of Sweet Potato Leaves

Molecules ◽

10.3390/molecules26071820 ◽

2021 ◽

Vol 26 (7) ◽

pp. 1820

Author(s):

Hoang Chinh Nguyen ◽

Chang-Chang Chen ◽

Kuan-Hung Lin ◽

Pi-Yu Chao ◽

Hsin-Hung Lin ◽

...

Keyword(s):

Sweet Potato ◽

Bioactive Compounds ◽

Ipomoea Batatas ◽

Current Knowledge ◽

Health Benefits ◽

Health Promoting ◽

New Compounds ◽

Sweet Potato Leaves

Sweet potato (Ipomoea batatas) is one of the most important food crops worldwide and its leaves provide a dietary source of nutrients and various bioactive compounds. These constituents of sweet potato leaves (SPL) vary among varieties and play important roles in treating and preventing various diseases. Recently, more attentions in health-promoting benefits have led to several in vitro and in vivo investigations, as well as the identification and quantification of bioactive compounds in SPL. Among them, many new compounds have been reported as the first identified compounds from SPL with their dominant bioactivities. This review summarizes the current knowledge of the bioactive compositions of SPL and their health benefits. Since SPL serve as a potential source of micronutrients and functional compounds, they can be further developed as a sustainable crop for food and medicinal industries.

Download Full-text

Anthocyanins from purple sweet potato alleviate doxorubicin‐induced cardiotoxicity in vitro and in vivo

Journal of Food Biochemistry ◽

10.1111/jfbc.13869 ◽

2021 ◽

Author(s):

Sixue Tang ◽

Juan Kan ◽

Rui Sun ◽

Huahao Cai ◽

Jinhai Hong ◽

...

Keyword(s):

Sweet Potato ◽

Purple Sweet Potato

Download Full-text

Reductions of copper ion-mediated low-density lipoprotein (LDL) oxidations of trypsin inhibitors, the sweet potato root major proteins, and LDL binding capacities

Botanical Studies ◽

10.1186/s40529-020-00303-4 ◽

2020 ◽

Vol 61 (1) ◽

Author(s):

Yeh-Lin Lu ◽

Chia-Jung Lee ◽

Shyr-Yi Lin ◽

Wen-Chi Hou

Keyword(s):

Sweet Potato ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Trypsin Inhibitors ◽

Water Soluble ◽

Affinity Column ◽

Low Density ◽

Native Page

Abstract Background The root major proteins of sweet potato trypsin inhibitors (SPTIs) or named sporamin, estimated for 60 to 80% water-soluble proteins, exhibited many biological activities. The human low-density lipoprotein (LDL) showed to form in vivo complex with endogenous oxidized alpha-1-antitrypsin. Little is known concerning the interactions between SPTIs and LDL in vitro. Results The thiobarbituric-acid-reactive-substance (TBARS) assays were used to monitor 0.1 mM Cu2+-mediated low-density lipoprotein (LDL) oxidations during 24-h reactions with or without SPTIs additions. The protein stains in native PAGE gels were used to identify the bindings between native or reduced forms of SPTIs or soybean TIs and LDL, or oxidized LDL (oxLDL). It was found that the SPTIs additions showed to reduce LDL oxidations in the first 6-h and then gradually decreased the capacities of anti-LDL oxidations. The protein stains in native PAGE gels showed more intense LDL bands in the presence of SPTIs, and 0.5-h and 1-h reached the highest one. The SPTIs also bound to the oxLDL, and low pH condition (pH 2.0) might break the interactions revealed by HPLC. The LDL or oxLDL adsorbed onto self-prepared SPTIs-affinity column and some components were eluted by 0.2 M KCl (pH 2.0). The native or reduced SPTIs or soybean TIs showed different binding capacities toward LDL and oxLDL in vitro. Conclusion The SPTIs might be useful in developing functional foods as antioxidant and nutrient supplements, and the physiological roles of SPTIs-LDL and SPTIs-oxLDL complex in vivo will investigate further using animal models.

Download Full-text