scholarly journals Free radical scavenging activity of taro (Colocasia esculenta (L.) Schott) varieties with varying flesh color

2020 ◽  
pp. 23-32
Author(s):  
Erlinda Vasquez ◽  
Precila Contero ◽  
Dilberto Ferraren
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reduction Rate ◽  
Free Radical Scavenging ◽  
Differential Method ◽  
Free Radical Scavenging Activity ◽  
Antioxidant Compounds ◽  
Scavenging Activity

Corms of five taro varieties namelyztwo NSIC-registered varieties, VG-1 (Kalpao) and VG-2 (Iniito), and three farmer’s variety, PRG 322 (Chowking), PRG 381 (Pandaga), and PRG 380 (Salot), were evaluated for antioxidant activity using 1, 1-diphenyl-2 picrylhydrazyl (DPPH) assay before and after cooking. The purple-fleshed varieties PRG 322, PRG 380 and VG-2 showed higher antioxidant activity than theyellow- and cream-fleshed varieties, The monomeric anthocyanin contents of PRG 380 and VG-1 PRG 322 and VG-2, which showed the highest free radical scavenging activity were measured using the pH differential method. The free» radical scavenging activity ofthe five taro varieties ranged from 134 to 154TEμm g-1 when raw and 127 to 147TEμm g-1 when cooked with the highest activity in raw PRG 322 followed by raw PRG 380 and VG-2. Cooked PRG 381 and VG-1 showed the lowest activity, Cooking reduced the activity by 4.5-10.2% with PRG 380 showing the highest reduction rate while the other four varieties did not vary significantly from 4.5-5.9%. Decreased in antioxidant activity can be due to the degradation and leaching out of antioxidant compounds during steamed cooking.

scholarly journals Antioxidant Activity of Moringa oleifera Extracts

2018 ◽  
Vol 16 (3) ◽  
pp. 297 ◽  
Author(s):  
Wiwit Denny Fitriana ◽  
Taslim Ersam ◽  
Kuniyoshi Shimizu ◽  
Sri Fatmawati
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Moringa Oleifera ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Assay ◽  
Abts Assay

Moringa oleifera have been evaluated for its antioxidant activity. M. oleifera leaves were extracted with methanol, ethyl acetate, dichloromethane and n-hexane. The antioxidant activity of extracts were evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity assay and an improved 2,2’-azino-bis-[3-ethylbenzothiazoline sulphonate] (ABTS) radical cation decolorization assay in vitro. Trolox was used as standard with IC50 5.89 μg/mL in DPPH assay and 3.06 μg/mL in ABTS assay. The methanol extract showed the highest free radical scavenging activity with IC50 value of 49.30 μg/mL in DPPH assay and 11.73 μg/mL in ABTS assay. This study provided that M. oleifera leaves possess antioxidant.

Antioxidant Activities of Yinchenhao (Artemisiae Capillaris) Leaves

2020 ◽  
Vol 18 (4) ◽  
pp. 386-391
Author(s):  
Yonghun Kim ◽  
Ang Li ◽  
Junyu Wang ◽  
Wancong Yu ◽  
Fang Wan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Therapeutic Effects ◽  
Scavenging Activity

Yinchenhao (Artemisia capillaris Thunb.) is a pharmaceutical agent that not only shows therapeutic effects against hepatobiliary diseases but also offers various physiological benefits. This study examined the extraction rates of antioxidant extracts from Yinchenhao leaves using different solvents. The extraction rates using different solvents were as follows: ethanol (12.1 ± 0.87%) > water (7.7 ± 0.45%) > n-butanol (1.3 ± 0.16%) ethyl acetate > (1.3 ± 0.14%) > n-hexane (1.1 ± 0.15%). The n-butanol and ethyl acetate extracts showed higher 1,1-Diphenyl-2 picrylhydrazyl radical free radical scavenging activity, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonate) free radical scavenging activity and ferric ion reducing antioxidant activity, while n-hexane extracts showed weak antioxidant activity. In conclusion, Yinchenhao leaf has potential as a natural antioxidant, and n-butanol and ethyl acetate may be effective extraction solvents for studying its antioxidant activities.

Free radicals scavenging aptitude of Spilanthes acmella

2021 ◽  
pp. 2631-2634
Author(s):  
K. Gouri Sankar ◽  
B. S. Venkateswarlu
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Bioactive Molecules ◽  
Scavenging Activity ◽  
Spilanthes Acmella

Now a days, oxidative stress is one of the major disease around the world. The excess production of oxidants in the body are key source for development of different chronic diseases and are leading to damage the multiple organs' function, finally to cellular mortality. The natural remedy is the most important medicine to control the overproduction of oxidants and to obtain more antioxidants. Natural product have been using to treat diseases and as food supplements as nutrients. In this point of view, the current study was carried to evaluate the antioxidant activity of Spilanthes acmella root parts. The free radical scavenging activity was studied against 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide and hydroxyl free radicals using standard methods. The Spilanthes acmella root part extracts showed concentration dependent antioxidant activity on tested free radicals. The extracts showed modest effect on free radicals compared to the standard drug ascorbic acid and hydro-alcoholic extract possesses more activity than other two extracts. The end results of current study clearly state that Spilanthes acmella root possess free radical scavenging activity and also confirms its' traditional value in traditional medicine. The further research is worthful on isolation of different bioactive molecules and different biological activities evaluation on Spilanthes acmella.

scholarly journals Evaluation of antioxidant activity of ormeloxifene: in vitro study

2017 ◽  
Vol 6 (10) ◽  
pp. 2507
Author(s):  
Vinay M. ◽  
Seethalakshmi S. ◽  
Vijay Kumar
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
No Synthase ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Assay

Background: Ormeloxifene (Centchroman) is a Selective Estrogen Receptor Modulator (SERM) which acts as estrogen antagonist and having anti progestogenic activity also. It is being used in the management of dysfunctional uterine bleeding and as nonhormonal oral contraceptive. It is also being investigated for the indications such as osteoporosis, breast and endometrial carcinoma. In this study, we have evaluated the Antioxidant potential of drug by using DPPH and NO synthase Assay. It was found that ormeloxifene has significant antioxidant activity which could be cause for its use in various gynaecological and other conditions.Methods: In this study, we have demonstrated in vitro antioxidant activity of ormeloxifene. DPPH and NO synthase assay tests were done for different concentrations of ormeloxifene.Results: In our study, it showed that the free radical scavenging activity of ormeloxifene was less in lower concentration and increased in the higher concentration in DPPH assay. The free radical scavenging activity of drug ormeloxifene was 22% at 100µg/ml and 27% for the concentrations of 1000µg/ml in DPPH assay. No scavenging activity was 3% at 100µg/ml and 11% at 1000µg/ml.Conclusions: The invitro antioxidant analysis of ormeloxifene, was proved to be a potent antioxidant.

scholarly journals Phenolic compounds and antioxidant activity of different parts of three mandarin varieties extracts: A comparative study

2020 ◽  
Vol 4 (8) ◽  
pp. 318-324
Author(s):  
Makhlouf Chaalal ◽  
iham Ydjedd ◽  
Sana Mansouri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Different Parts

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel source of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels reveled a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.

scholarly journals Hypotensive effect of Cichorium intybus extract in rats

2021 ◽  
Vol 10 (2) ◽  
pp. 257-261
Author(s):  
Mehrnoosh Sedighi ◽  
Mostafa Cheraghi ◽  
Mahdieh Faghihi ◽  
Mohammad Rahimi-Madiseh ◽  
Ali Asghar Kiani ◽  
...  
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Cichorium Intybus ◽  
Free Radical Scavenging Activity ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Scavenging Activity ◽  
Phenolic And Flavonoid

Introduction: Oxidative stress is involved in many diseases, including hypertension, kidney failure, and heart disease. This study aimed to evaluate the effect of hydroalcoholic Cichorium intybus extract on blood pressure in rats. Antioxidant activity, phenolic and flavonoid contents of the plant extract were also evaluated. Methods: In this study, 32 male Wistar rats weighing 250-300 g were divided into four groups of eight each. Animals in the control group were administered with normal saline and in the C. intybus groups with extract at 25, 50, and 100 mg/kg for two weeks. Then, the homodynamic parameters were examined by the Power lab. The phenolic and flavonoid contents were also evaluated by a spectrophotometer and the rate of free radical scavenging activity was measured by the diphenyl-1-picyryl-hydrazyl (DPPH) free radical method. Results: The free radical scavenging activity of C. intybus extract was obtained 47.85% of DPPH, and flavonoid and phenolic contents were 8.21 and 27.19 mg/g of dry extract, respectively. Meanwhile, median (MAP), systolic (SAP) and diastolic arterial pressure (DAP) significantly decreased in the 50 mg/kg extract-treated group compared to the control and 200 mg/kg extract-treated groups. Conclusion: Ethanol extract of C. intybus plays a protective role against hypertension, which, in part, might be due to antioxidant compounds of the plant against free radicals.

scholarly journals Phenolic compounds and antioxidant activity of different parts of three mandarin varieties extracts: A comparative study

2020 ◽  
Vol 4 (08) ◽  
pp. 318-324
Author(s):  
Makhlouf Chaalal ◽  
Siham Ydjedd ◽  
Sana Mansouri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Phenolic Compounds ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Different Parts

Background: Mandarin by-products, such as peels and seeds, are considered as one of the naturel sources of phenolic compounds. Aim: The objective of the present study was to compare the phenolic compounds contents and antioxidant activity of different parts (pulps, seeds, juices, and peels) of three mandarins varieties extracts. Material and methods: Total phenolic, flavonoid, and proanthocyanidin contents of the extracts were assessed while, the antioxidant activity was evaluated using three tests including ferric reducing power, free radical scavenging activity, and phosphomolybdate. Results: The Mediterranean Mandarin peels present a high total phenolics and flavonoids contents with values of 2445.62 mg GAE/100g FW and 609.78 mg QE/100g FW, respectively. Likewise, the clementine juice showed the highest proanthocyanins contents with a value of 46.67 mg CE/100g FW. Seeds and pulps of the three varieties present the lowest phenolic compound contents. Mediterranean Mandarin juice showed a strong ferric reducing power and phosphomolybdate with values of 1270.91 and 190.19 mg AAE/100g FW, respectively, however, the peels revealed a strong free radical scavenging activity with value of 259.89 mg AAE/100 g FW. Statistical analysis showed a positive correlation between the antioxidant activity and the phenolic compounds contents of the three studied mandarins varieties extracts. Conclusion: Among the analyzed cultivars, the Mediterranean mandarin variety may be considered as the most promising source of polyphenols and antioxidants, compared to the clementine and Satsuma varieties. Moreover, this study also highlights important properties of the fruit parts generally considered wastes. Keywords: Mandarins, different parts, phenolics compounds, antioxidant activity.

DETERMINATION OF BIOACTIVE CONTENTS AND IN VITRO ANTIOXIDANT ACTIVITY OF POLY HERBAL FORMULATIONS

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (05) ◽  
pp. 74-79
Author(s):  
A. K Srivastava ◽  
D. Kaushik ◽  
V. K. Lal
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vitro Models ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Cardiac Complications ◽  
Scavenging Activity

Free radicals are reactive molecules involved in many physiological processes and have been associated with many diseases, such as ageing, cancer, arthritis and liver injury and cardiac complications. In polyherbal formulations HAF-I and HAF-II, described below, the total phenolics content were found to be 34.4±0.10 and 27.6±1.20 mg gallic acid equivalent (GAE)/g and total flavonoids contents, total tannin contents were 24.7±0.25 and 18.1±1.20 RE/g and 12.31±0.25 and 9.48±1.85 GAE/g, respectively. Free radical scavenging activity was determined according to the elimination of DPPH radicals. Total phenol content was determined by the Folin–Ciocalteu reaction. The relative antioxidant ability of the polyherbal formulations were investigated through two in vitro models, namely, antioxidant capacity by radical scavenging activity using α, α-diphenyl-β-picrylhydrazyl (DPPH) and nitric oxide (NO) methods. The extracts were used at 20, 40, 60, 80 and 100 μg/mL concentrations and radical scavenging activity was determined in terms of inhibition percentage. The IC50 (concentration required for 50% inhibition) were calculated for each radical. The present study was designed to evaluate the free radical scavenging activity of hydro-alcoholic extracts of polyherbal formulations (HAF-I & HAF-II) various in-vitro models using ascorbic acid and rutin as references. The in vitro free radical DPPH activities were found to be 74.17±0.18 & 75.30±0.18 and NO antioxidant activity were found to be 75.3±1.10 & 76.17±1.24 at maximum concentration of 100 μg/mL. The in-vitro anti-oxidant activity of these polyherbal formulations may be due to the presence of polyphenols.

GC-MS Analysis and In Vitro Antioxidant Activity of Crude Extracts and Different Fractions of Moringa Oleifera Pods

2021 ◽  
Vol 1025 ◽  
pp. 247-251
Author(s):  
Hasriana Hadi ◽  
Nur Salsabila Ahmad Roslan ◽  
Normaiza Zamri
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Moringa Oleifera ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Chemical Compositions ◽  
Scavenging Activity

Moringa oleifera (M. oleifera) has been known as a medicinal plant. The objectives of this study is to identify the chemical compositions of M. oleifera pods extracts and to evaluate its total phenolic content (TPC) and antioxidant activity. The methodology of this study began with the collection of the pod's sample, drying and grinding the pods into powdered form. M. oleifera pods extracts were prepared by using methanol (MeOH), hexane, dichloromethane (DCM), and ethyl acetate (EtOAc) solvent which yielded respective residues. All extracts were subjected to Gas Chromatography-Mass Spectrometry (GC-MS) to identify its chemical compositions. Major compound found in hexane, DCM, EtOAc, and MeOH extracts were nonanoic acid (30.94 %), diisooctyl phthalate (89.45 %), benzoic acid, 4-hydroxy- (37.57 %) and octanoic acid (19.19 %), respectively. The TPC quantification was done via Folin-Ciocalteu method showed that EtOAc extract has the highest phenols content with 500.05 mg GAE/g. Generally, all extract contain moderate phenolic content. M. oleifera pods extracts were screened for the antioxidant activity via 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical. Hexane, DCM and EtOAc extracts exhibited good free radical scavenging activity with IC50 of 65.79 μg/mL, 74.93 μg/mL and 82.83 μg/mL, respectively. MeOH showed the lowest free radical scavenging activity with IC50 529.6 μg/mL. However, antioxidant activity of all extracts was low compared to standard, ascorbic acid (AA) with IC50 of 19.57 μg/mL. The presence of various bioactive constituents in the M. oleifera pods extracts might contributed to the antioxidant activity of the plant.

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