Rheological Properties of İnjectable Hyaluronic Acid Hydrogels for Soft Tissue Engineering Applications

Hydrogels are cross-linked three-dimensional (3D) polymeric network, which can hold the water within its porous structure. They have recently been used in various biomedical applications. In this study, injectable hyaluronic acid (HA) hydrogels were prepared using different concentrations of 1,4-butanediol diglycidyl ether (BDDE) as a crosslinker (1-5% w/w) and investigated their rheological, swelling and injectability properties. The results demonstrated that the rheological characteristics of hydrogels enhanced with increasing crosslinker concentration. The elastic modulus of the hydrogels ranged from 280 Pa to 990 Pa, while the complex viscosities were found between 42 Pa.s and 190 Pa.s at an oscillation frequency of 1 Hz. These results clearly suggest that the injectable HA hydrogels are a potential candidate for various soft tissue engineering applications due to their highly tunable rheological properties.

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Tunable methacrylated hyaluronic acid‐based hydrogels as scaffolds for soft tissue engineering applications

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.36814 ◽

2019 ◽

Vol 108 (2) ◽

pp. 279-291 ◽

Cited By ~ 9

Author(s):

Benjamin S. Spearman ◽

Nikunj K. Agrawal ◽

Andrés Rubiano ◽

Chelsey S. Simmons ◽

Sahba Mobini ◽

...

Keyword(s):

Tissue Engineering ◽

Hyaluronic Acid ◽

Soft Tissue ◽

Engineering Applications ◽

Soft Tissue Engineering

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Biodegradable hydrogels composed of oxime crosslinked poly(ethylene glycol), hyaluronic acid and collagen: a tunable platform for soft tissue engineering

Journal of Biomaterials Science Polymer Edition ◽

10.1080/09205063.2014.975393 ◽

2015 ◽

Vol 26 (3) ◽

pp. 143-161 ◽

Cited By ~ 33

Author(s):

John G. Hardy ◽

Phillip Lin ◽

Christine E. Schmidt

Keyword(s):

Tissue Engineering ◽

Hyaluronic Acid ◽

Soft Tissue ◽

Ethylene Glycol ◽

Poly Ethylene Glycol ◽

Biodegradable Hydrogels ◽

Soft Tissue Engineering ◽

Poly Ethylene

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Three-Dimensional Printable Hydrogel Using a Hyaluronic Acid/Sodium Alginate Bio-Ink

Polymers ◽

10.3390/polym13050794 ◽

2021 ◽

Vol 13 (5) ◽

pp. 794 ◽

Cited By ~ 1

Author(s):

Su Jeong Lee ◽

Ji Min Seok ◽

Jun Hee Lee ◽

Jaejong Lee ◽

Wan Doo Kim ◽

...

Keyword(s):

Tissue Engineering ◽

Hyaluronic Acid ◽

Sodium Alginate ◽

Three Dimensional ◽

Fibroblast Cell ◽

Chemical Crosslinking ◽

Fibroblast Cell Line ◽

Engineering Applications ◽

Encapsulated Cells ◽

Crosslinking Agents

Bio-ink properties have been extensively studied for use in the three-dimensional (3D) bio-printing process for tissue engineering applications. In this study, we developed a method to synthesize bio-ink using hyaluronic acid (HA) and sodium alginate (SA) without employing the chemical crosslinking agents of HA to 30% (w/v). Furthermore, we evaluated the properties of the obtained bio-inks to gauge their suitability in bio-printing, primarily focusing on their viscosity, printability, and shrinkage properties. Furthermore, the bio-ink encapsulating the cells (NIH3T3 fibroblast cell line) was characterized using a live/dead assay and WST-1 to assess the biocompatibility. It was inferred from the results that the blended hydrogel was successfully printed for all groups with viscosities of 883 Pa∙s (HA, 0% w/v), 1211 Pa∙s (HA, 10% w/v), and 1525 Pa∙s, (HA, 30% w/v) at a 0.1 s−1 shear rate. Their structures exhibited no significant shrinkage after CaCl2 crosslinking and maintained their integrity during the culture periods. The relative proliferation rate of the encapsulated cells in the HA/SA blended bio-ink was 70% higher than the SA-only bio-ink after the fourth day. These results suggest that the 3D printable HA/SA hydrogel could be used as the bio-ink for tissue engineering applications.

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