scholarly journals Synergistic Interaction Between Cationic Novel Gemini Surfactant and Non-ionic Conventional Surfactants in Aqueous Medium

2021 ◽  
Vol 12 (6) ◽  
pp. 7416-7428
Keyword(s):  
Aqueous Medium ◽  
Gemini Surfactant ◽  
Conductometric Titration ◽  
Theoretical Models ◽  
Tween 20 ◽  
Ammonium Bromide ◽  
Ideal Behavior ◽  
Synergistic Behavior ◽  
Triton X ◽  
Mixed Systems

The interaction between novel gemini surfactant (alkane-α, ω-bis(dimethyl hexadecyl ammonium bromide) (G6)) and two different non-ionic surfactants (Triton X-114 (TX-114), Tween 20 (T-20)) has been investigated in the current study at temperature 298.15 K. The critical micelle concentration (cmc) values of mixed systems were computed by the conductometric titration in the aqueous medium. The results have been examined by utilizing numerous theoretical models (Clint, Rubingh, Motomura, Maeda, and Lange models). The experimentally determined cmc were lower than corresponding ideal values (cmc*) and decreased with the mixtures' stoichiometric mole fraction (α1) of G6. This shows non-ideal behavior between two employed components. The interaction parameter at mixed micelle (β) has been analyzed by using regular solution approximation. The attractive interaction or synergistic behavior in both mixed systems is confirmed by the negative values of β. The various energetics parameters were also evaluated and discussed.

The efficiency of various detergents for extraction and stabilization of acetylcholinesterase from bovine erythrocytes

1987 ◽  
Vol 65 (1) ◽  
pp. 8-18 ◽  
Author(s):  
Rex K. M. Wong ◽  
Christine P. Nichol ◽  
M. Chandra Sekar ◽  
Basil D. Roufogalis
Keyword(s):  
Chain Length ◽  
Membrane Lipids ◽  
Acetylcholinesterase Activity ◽  
Exchange Chromatography ◽  
Erythrocyte Membranes ◽  
Tween 20 ◽  
Bovine Erythrocyte ◽  
Critical Micelle Concentrations ◽  
Triton X ◽  
Nonionic Detergents

The efficiency of several nonionic detergents and a homologous series of zwitterionic detergents for the extraction of acetylcholinesterase (EC 3.1.1.7) from bovine erythrocyte membranes was examined. Of the nonionic detergents examined, the polyoxyethylene-based Tweens were the least effective solubilizing agents. Within this series, increasing the length of the saturated fatty acid chain progressively decreased the efficiency of enzyme recovery, while unsaturation in the side chain reversed this trend. In the Lubrol detergents, where the chain length of the alcohol group is variable, an increase in the length of the polyoxyethylene glycol group decreased the recovery of acetylcholinesterase in the solubilized state, without affecting the efficiency of extraction of total erythrocyte protein. As with the other nonionic detergents examined, Triton X-100 and octy1 β-D-glucoside were maximally effective in solubilizing acetylcholinesterase activity at concentrations greater than their respective critical micelle concentrations. In the sulfobetaine (N-alkyldimethylaminopropane sulphonate) zwitterionic detergent series, the longer alkyl chain zwittergents Z 316 and Z 314 were more efficient than the shorter chain length members of the series (Z 310 and Z 312). In contrast to the higher chain length compounds, short chain analogs were maximally effective at or below their critical micelle concentrations. After purification by ion-exchange chromatography and affinity chromatography, the enzyme extracted with the various detergents gave sedimentation coefficients between 6.8S and 7.6S, consistent with a dimeric structure. Acetylcholinesterase could also be efficiently released by 0.2 mM EDTA or 0.5 M NaCl from bovine erythrocyte membranes previously depleted of 70–80% of the membrane lipids by butanol. Nonlinear Arrhenius plots of enzyme activity were found whether acetylcholinesterase was solubilized with Tween 20, Lubrol PX, or Triton X-100. The present work confirms that bovine erythrocyte acetylcholinesterase requires detergents to solubilize it from membranes and that its activity depends on the structure of the amphiphiles used to solubilize the enzyme.

scholarly journals Mucin biosynthesis. Properties of a bovine tracheal mucin β-6-N-acetylglucosaminyltransferase

1985 ◽  
Vol 227 (2) ◽  
pp. 405-412 ◽  
Author(s):  
P W Cheng ◽  
W E Wingert ◽  
M R Little ◽  
R Wei
Keyword(s):  
Enzyme Activity ◽  
Freezing Point ◽  
Cetylpyridinium Chloride ◽  
Sodium Dodecyl ◽  
Sodium Deoxycholate ◽  
Gas Chromatography Mass Spectrometry ◽  
Tween 20 ◽  
Group A ◽  
Michaelis Constants ◽  
Triton X

We have characterized a bovine tracheal mucin beta-6-N-acetylglucosaminyltransferase that catalyses the transfer of N-acetylglucosamine from UDP-N-acetylglucosamine to the C-6 of the N-acetylgalactosamine residue of galactosyl-β 1→3-N-acetylgalactosamine. Optimal enzyme activity was obtained between pH 7.5-8.5, at 5mM-MnCl2, and at 0.06-0.08% (v/v) Triton X-100 (or Nonidet P-40), or 0.5-5.0% (v/v) Tween 20. Ba2+, Mg2+ and Ca2+ could partially replace Mn2+, but Co2+, Fe2+, Cd2+ and Zn2+ could not. Sodium dodecyl sulphate, cetylpyridinium chloride, sodium deoxycholate, octyl beta-D-glucoside, digitonin and alkyl alcohols were less effective in enhancing enzyme activity, and dimethyl sulphoxide was ineffective. The apparent Michaelis constants were 1.25 mM for UDP-N-acetylglucosamine, 0.94-3.34 mM for freezing-point-depressing glycoprotein and 0.19 mM for periodate-treated blood-group-A porcine submaxillary mucin. Asialo ovine submaxillary mucin could not serve as the glycosyl acceptor. The structure of the 14C-labelled oligosaccharide obtained by alkaline-borohydride treatment of the product was identified as Gal beta 1→3(Glc-NAc beta 1→6)N-acetylgalactosaminitol by beta-hexosaminidase treatment, gas chromatography-mass spectrometry and 1H-n.m.r. (270 MHz) analysis. The enzyme is important in the regulation of mucin oligosaccharide biosynthesis.

Drug Solubilization by Surfactants: Experimental Methods and Theoretical Perspectives

2021 ◽  
Vol 21 ◽  
Author(s):  
Nisar Ahmad Malik
Keyword(s):  
Theoretical Models ◽  
Experimental Methods ◽  
Poorly Soluble Drugs ◽  
Drug Solubilization ◽  
Theoretical Perspectives ◽  
Surface Active Agents ◽  
Poorly Soluble ◽  
Surface Active ◽  
Mixed Systems ◽  
Insight Into

: This mini review will give an insight into the need and usefulness of investigating the solubilization of poorly soluble drugs. Commonly used experimental and theoretical models are outlined to study the efficacy of the carrier or excipient for the poorly soluble drugs. Furthermore, the use of surface active agents for drug solubilization is discussed in correlation with the mathematical models suggested from time to time. A few experimental techniques are also discussed which would be very helpful in elucidating the interactions prevailing in the mixed systems of poorly soluble drugs and surface active agents.

Effect of Polyacrylic Acid and Polyacrylamide on Clouding Behavior of Triton-X-100 in Aqueous Medium

2015 ◽  
Vol 52 (1) ◽  
pp. 62-66 ◽  
Author(s):  
Anil D. Kakuste ◽  
Mahendra S. Borse ◽  
Gunvant H. Sonawane
Keyword(s):  
Aqueous Medium ◽  
Polyacrylic Acid ◽  
Triton X

Metal-free synthesis of polysubstituted pyrroles using surfactants in aqueous medium

2017 ◽  
Vol 19 (22) ◽  
pp. 5385-5389 ◽  
Author(s):  
Amrendra Kumar ◽  
Ramanand Ramanand ◽  
Narender Tadigoppula
Keyword(s):  
Aqueous Medium ◽  
Sodium Dodecyl Sulphate ◽  
Room Temperature ◽  
Sodium Dodecyl ◽  
Pyrrole Derivatives ◽  
Metal Free ◽  
Triton X

An efficient and metal-free method has been developed for the synthesis of polysubstituted pyrrole derivatives with combination of sodium dodecyl sulphate (SDS) and Triton X-100 surfactants using water as a solvent at room temperature in 2–6 h and under microwave conditions (10 min) with good to excellent yields.

Interactions between bovine serum albumin and gemini surfactant alkanediyl-α, ω-bis(dimethyldodecyl-ammonium bromide)

Biopolymers ◽  
2006 ◽  
Vol 83 (3) ◽  
pp. 243-249 ◽  
Author(s):  
Yingying Pi ◽  
Yazhuo Shang ◽  
Changjun Peng ◽  
Honglai Liu ◽  
Ying Hu ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Gemini Surfactant ◽  
Ammonium Bromide

scholarly journals A Novel Method for the Determination of Synthetic Colors in Ice Cream Samples

2004 ◽  
Vol 87 (3) ◽  
pp. 657-663 ◽  
Author(s):  
Meenakshi Tripathi ◽  
Subhash K Khanna ◽  
Mukul Das
Keyword(s):  
Indigo Carmine ◽  
Ice Cream ◽  
Trisodium Citrate ◽  
Ammonia Solution ◽  
Tween 20 ◽  
Sunset Yellow ◽  
Simple Method ◽  
Paper Chromatogram ◽  
Triton X

Abstract A simple method has been developed for the extraction, separation, and determination of synthetic colors in ice cream samples. The process involves the breakdown of emulsion by neutral detergents (Triton X-100 and Tween 20) followed by extraction with petroleum ether for removal of fat. The aqueous colored solution obtained is treated with 5% acetic acid, and the uptake of color is carried out by a wool-dyeing technique. The color is eluted from the wool with 5% ammonia solution, the solution is evaporated to dryness, and the residue is dissolved in 60% ethanol for paper chromatography using trisodium citrate–ammonia–water (2 + 5 + 95, w/v/v) as the mobile phase. The colored spots from the paper chromatogram are cut and eluted with 60% ethanol, and the absorbance is measured at the respective λ maximum corresponding to the Rf value of the appropriate standard. The recoveries of 6 colors, including sunset yellow FCF (SSYFCF), tartrazine, carmoisine, ponceau 4R, brilliant blue FCF (BBFCF), and fast green FCF from spiked samples with either detergent were found to be >90%. However, recoveries of erythrosine were 21 and 65% with Triton X-100 and Tween 20, respectively. Indigo carmine could not be recovered at all because of its fugitive property in 5% ammonia solution, which is used to strip the color from the wool. The sensitivity of the method with the use of Tween 20 is 1 ppm (1 μg/g) for the colors in spiked ice cream samples. With this method, we analyzed samples of 20 branded colored ice cream. The results showed the presence of tartrazine (8.4–43.3 ppm), SSYFCF (23.5–117.6 ppm), carmoisine (traces–53.2 ppm), erythrosine (3.5 ppm), and BBFCF (4.1 ppm) in the ice cream samples. Apart from 2 samples of tuttifruity, all of the ice cream samples showed the presence of permitted synthetic colors below the permissible level of 100 ppm established by the Prevention of Food Adulteration Act of India.

Detergentes: Triton X-100, Tween-20 e mais

2013 ◽  
Vol pt3 ◽  
Author(s):  
Mary Johnson
Keyword(s):  
Tween 20 ◽  
Triton X
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