Effect Of Ga3 And Row Ratio On Floral Traits Of Component Lines Of BRRI Hybrid Dhan2

Component lines (A and R lines) of BRRI hybrid dhan2 were assessed under treatment of GA3 application and row ratio on days to 5% and 50% flowering, duration of opening of floret, angle of open floret, filament length, anther length, stigma length, panicle exsertion rate, stigma exsertion rate and outcrossing rate. The CMS line viz; BRRI 10A have been found to be usable female parents for hybrid rice seed production due to their stigma length, stigma exsertion rate, duration of blooming, anther length and filament length. The different between genotypic and phenotypic variation was not remarkable for most of the characters studied indicating negligible environmental influence on the traits. The maximum duration of opening of floret (158.10 min) and angle of floret opening (28.870) were obtained with the application of 350 g GA3 ha-1. The improvement in the floral traits with increased GA3 level might be due to increased availability of GA3 which enhanced floral traits. The CMS line can be used as potential female parent in hybrid rice seed production of BRRI hybrid dhan2. Bangladesh J. Agril. Res. 38(1): 155-163, March 2013 DOI: http://dx.doi.org/10.3329/bjar.v38i1.15202

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Detection of QTLs for Outcrossing-Related Traits in CSSL Population Derived from Primitive Japonica Accession Ludao in the Genetic Background of O. sativa spp. Japonica Restorer C-bao Using RSTEP-LRT Method

Agronomy ◽

10.3390/agronomy10010028 ◽

2019 ◽

Vol 10 (1) ◽

pp. 28

Author(s):

Lal Bux ◽

Dalu Li ◽

Muhammad Faheem ◽

Nour Ali ◽

Muzafar Hussain Sirohi ◽

...

Keyword(s):

Seed Production ◽

Hybrid Rice ◽

Oryza Sativa L ◽

Flag Leaf ◽

Leaf Angle ◽

Ratio Test ◽

Phenotypic Variance ◽

Rice Seed ◽

Hybrid Seed Production ◽

Cms Line

The outcrossing traits in rice (Oryza sativa L.) affect the yield of hybrid seed production. Using a cytoplasmic male sterile (CMS) line with good outcrossing traits, such as short flag leaf length (FLL), narrow flag leaf width (FLW), wide flag leaf angle (FLA), and elongated panicle neck length (PNL), for hybrid rice seed production, it is possible to avoid the procedure of cutting flag leaves and make the supplementary pollination feasible by machine. In this study, a japonica restorer C-bao as the receptor parent and a primitive japonica accession Ludao as the donor parent were used to construct a chromosome segment substitution line (CSSL) population. The CSSL population was used to detect quantitative trait loci (QTLs) for the four outcrossing traits using a likelihood ratio test based on the stepwise regression (RSTEP-LRT) method. The CSSL population constructed consisted of 163 lines covering 90.7% of the donor genome. Among the seven QTLs detected in the CSSL population, four QTLs were detected in both years. qFLL-4 explained 6.70% of the two-year-averaged phenotypic variance, and the alleles from Ludao decreased FLL 5.1 cm. qFLA-1.1 and qFLA-1.2 explained 7.85% and 21.29% of the 2-year-averaged phenotypic variance respectively, and the alleles from Ludao increased FLA 17.38° and 31.50°. qPNL-8 explained 8.87% of the 2-year-averaged phenotypic variance, and the alleles from Ludao increased PNL 4.44 cm. These favorable alleles identified could be used to improve the outcrossing traits of parents for hybrid rice seed production in rice.

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Comparative study of floral characteristics in the component lines of hybrid rice (Oryza sativa L.)

Bangladesh Journal of Botany ◽

10.3329/bjb.v43i1.19739 ◽

2014 ◽

Vol 43 (1) ◽

pp. 1-8

Author(s):

MJ Hasan ◽

Umma Kulsum ◽

MH Rahman ◽

A Akter ◽

AKM Shamsuddin

Keyword(s):

Oryza Sativa ◽

Hybrid Rice ◽

Oryza Sativa L ◽

Floral Traits ◽

Restorer Line ◽

Hybrid Seed Production ◽

Filament Length ◽

Style Length ◽

Maintainer Line ◽

Anther Length

Significant variations were obtained among the component lines for most of the floral traits. BRRI9A exhibited superiority for angle of open florets, stigma length and breadth, anther breadth, filament length, style length and panicle exertion rate while its corresponding maintainer lines BRRI9B showed superiority for duration of open florets, stigma length and breadth, anther breadth and filament length. This indicated BRRI9A and its corresponding maintainer line BRRI9B possessed very good floral traits that influence out crossing. Among the restorer lines BR168R showed superiority for duration of open florets, angle of open florets, duration of bloomed florets/panicle, stigma length, anther length and number of pollen/microscopic focus. This suggested BR168R could be used as promising restorer line with BRRI9A for hybrid seed production in local condition.DOI: http://dx.doi.org/10.3329/bjb.v43i1.19739 Bangladesh J. Bot. 43(1): 1-8, 2014 (June)

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ROLE OF MORPHOLOGICAL TRAITS ABOVE FLAG LEAF NODE AND FLORAL TRAITS IN HYBRID RICE SEED PRODUCTION

Journal of Sustainable Agricultural Sciences ◽

10.21608/jsas.2017.1872.1029 ◽

2018 ◽

Vol 0 (0) ◽

pp. 0-0

Author(s):

Elsayed Arafat

Keyword(s):

Seed Production ◽

Morphological Traits ◽

Hybrid Rice ◽

Flag Leaf ◽

Leaf Node ◽

Floral Traits ◽

Rice Seed

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Floral traits and flowering behaviors of Malaysian rice cytoplasmic male sterile and maintainer lines and its relationship with out-crossing performance

Australian Journal of Crop Science ◽

10.21475/ajcs.21.15.02.p1732 ◽

2021 ◽

pp. 180-186

Author(s):

Shahida Hashim ◽

Phebe Ding ◽

Mohd Firdaus Ismail ◽

Asfaliza Ramli

Keyword(s):

Seed Production ◽

Hybrid Rice ◽

Agricultural Research ◽

Floral Traits ◽

Rice Seed ◽

Male Sterile ◽

Floral Trait ◽

Cytoplasmic Male Sterile ◽

Cross Pollination ◽

Parental Lines

Rice is a strictly self-pollinating crop. However, in hybrid rice seed production, an effective male sterility system is used to produce hybrid seed in bulk. In hybrid rice system, the pollen grains of cytoplasmic male sterile (CMS) are sterile and the female organ of the CMS depends on the fertile pollen released by the maintainer or restorer lines via out-crossing or cross-pollination in order to produce seed. Floral trait and flowering behavior of CMS and its corresponding maintainer or restorer lines are essential factors in hybrid rice seed production because they influenced the out-crossing or cross-pollination between parental lines. Two local CMSs and their corresponding maintainer lines were developed through breeding program in Malaysian Agricultural Research and Development Institute (MARDI) namely 0025A/0025B and 0047A/0047B. This study was carried out on floral traits and flowering behavior of these two hybrid line. Present studies have shown that there were variations between the CMS and its maintainer lines whether on floral trait or flowering behavior for both hybrid rice combinations. The results showed that stigma characters for both 0025A and 0047 were superior than their respective maintainers. Therefore, it is expected that the out-crossing rate would be high. Seeding date intervals need to be done on 0025A/0025B during nursery stage because the on-set of flowering between parental lines was significantly different. Panicle of both CMS was also classified as just exserted and partially-exserted and application of exogenous hormones such as gibberellic acid was useful to improve panicle elongation and consequently increase the seed set and yield. Correlation study indicates that the stigma area of both 0025A and 0047A has significant positive correlation with out-crossing rate.

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Heterosis and Combining Ability For Floral and Yield Characters in Rice Using Cytoplasmic Male Sterility System

10.21203/rs.3.rs-927164/v1 ◽

2021 ◽

Author(s):

Essam A. Z. ElShamey ◽

Raghda M. Sakran ◽

Mahmoud A. A. ElSayed ◽

Mahmoud E. Selim ◽

Mousa O. Germoush ◽

...

Keyword(s):

Grain Yield ◽

Cytoplasmic Male Sterility ◽

Male Sterility ◽

Floral Traits ◽

Hybrid Seed Production ◽

Gene Effects ◽

Environmental Pressures ◽

Anther Length ◽

Cms Line ◽

Stigma Exsertion

Abstract Developing high-yielding rice genotypes has become more urgent to ensure global food security with continuing population growth and the threat of environmental pressures. Cytoplasmic male sterility (CMS) system provides a valuable approach for commercial exploitation of heterosis and producing high-yielding and quality hybrid rice. Three CMS lines and ten diverse restorers were crossed using line × tester mating design. The obtained thirty F1 hybrids and their thirteen parents were evaluated. Yield traits as well as certain floral traits characters that influence the efficiency of crossing and hybrid seed production as duration of floret opening (min), stigma exsertion (mm), stigma length (mm), opening floret angle, and anther length (mm) were assessed. Highly significant variations were detected among parents, crosses, and parents vs. crosses for all the studied traits. The CMS line L2 and the restorer T5 were identified as good combiners for stigma exsertion, stigma length, opining floret angle, and duration of floret opening. Moreover, the hybrids L1×T1, L1×T3, L2×T2, L2×T5, L3×T4, L3×T5, and L3×T9 had positive SCA effects for most floral traits. Besides, the CMS lines L1 and L3 as well as the restorers T1, T2, T3, T6, and T9 proved to be the best general combiners for grain yield and certain contributing traits. The hybrids L1×T1, L1×T5, L1×T7, L2×T3, L2×T4, L2×T5, L2×T10, L3×T1, L3×T2, and L3×T6 displayed positive SCA effects for grain yield and one or more of its attributes. Both additive and non-additive gene effects are involved in the governing inheritance of all evaluated traits. The biochemical variations among the certain evaluated genotypes were further studied. The esterase and peroxidase isozymes were applied for verifying the genetic diversity at the protein level among the used CMS lines, restorers, and their crosses. All the applied isozymes displayed polymorphism for the parents and their crosses. The banding pattern and intensity differences provided accurate results on the reliable variability among the tested genotypes.

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