scholarly journals Determination of pollen viability and in vitro pollen germination of Rosa dumalis and Rosa villosa

1970 ◽  
Vol 36 (2) ◽  
pp. 185-187 ◽  
Author(s):  
Sezai Ercisli
Keyword(s):  
Key Words ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Genotype 1 ◽  
Genotype 4 ◽  
Pollen Quantity

Pollen quantity, viability and germination of four genotypes of Rosa dumalis and R. villosa were investigated. The number of anthers per flower were 90.3 in genotype 4 (Rosa villosa) and 116.4 in genotype 1 (Rosa dumalis). Genotype 1 showed the highest pollen viability on the basis of both TTC and IKI tests.   Key words: Dogrose, Pollen germination, Pollen viability, Rosa spp. DOI = 10.3329/bjb.v36i2.1511 Bangladesh J. Bot. 36(2): 185-187, 2007 (December)

scholarly journals Determination of Pollen Viability in Tomatoes

1992 ◽  
Vol 117 (3) ◽  
pp. 473-476 ◽  
Author(s):  
Aref A. Abdul-Baki
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Tube Growth ◽  
Percent Germination ◽  
Total Pollen ◽  
Highly Correlated

A procedure is described for determining pollen viability in tomatoes (Lycopersicon esculentum Mill.) by growing pollen in a growth medium containing 0.29 M sucrose, 1.27 mm Ca(NO3)2, 0.16 mm H3 BO3, and 1 mm KNO3 (pH 5.2) to which 0.001% fluorescein diacetate (FDA) was added. Pollen viability can be evaluated within 30 min by determining percent fluorescing pollen in a sample. The procedure further allows the determination of percent germination in vitro and pollen tube growth within 1.5 hours. Neither the germination medium nor FDA has any adverse effects on germination and pollen tube growth. Percent fluorescent pollen and percent total pollen germination were highly correlated, suggesting that fluorescence is a good measure of pollen viability. The combined fluorescence-germination procedure is simple and adapted to routine screening of many samples.

scholarly journals Foliar Application of Boron to Almond Trees Affects Pollen Quality

2000 ◽  
Vol 125 (2) ◽  
pp. 265-270 ◽  
Author(s):  
A.M.S. Nyomora ◽  
P.H. Brown ◽  
K. Pinney ◽  
V.S. Polito
Keyword(s):  
Pollen Germination ◽  
Foliar Application ◽  
Culture Media ◽  
Pollen Viability ◽  
Prunus Dulcis ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Mature Trees

The effect of boron (B) on in vivo and in vitro development of almond [Prunus dulcis (Mill.) D.A. Webb (syn. P. amygdalus Batsch)] pollen and pollen tubes and the resultant effect on fruit set was studied in mature trees. The cultivars Mono (pistil donor) and Butte (pollinizer) in an orchard with low soil B in Fresno, California were sprayed with B at 0, 0.8, 1.7, or 2.5 kg·ha-1 during Fall 1993. Pollen viability as indicated by the fluorescein diacetate method (FDA) was >85% and was not affected by field-applied B, however, in vivo pollen germination and tube growth were enhanced by foliar-applied B. More effect of applied B on in vivo growth appeared as pollen tubes progressed toward the ovary. For in vitro germination, foliar-applied B reduced bursting of tubes, and addition of B to the culture media significantly increased pollen germination and pollen tube growth.

scholarly journals Effect of high temperature on the reproductive development of chickpea genotypes under controlled environments

2012 ◽  
Vol 39 (12) ◽  
pp. 1009 ◽  
Author(s):  
Viola Devasirvatham ◽  
Pooran M. Gaur ◽  
Nalini Mallikarjuna ◽  
Raju N. Tokachichu ◽  
Richard M. Trethowan ◽  
...  
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Pollen Grains ◽  
High Temperature Stress ◽  
Pollen Production ◽  
Seed Number ◽  
Controlled Environments

High temperature during the reproductive stage in chickpea (Cicer arietinum L.) is a major cause of yield loss. The objective of this research was to determine whether that variation can be explained by differences in anther and pollen development under heat stress: the effect of high temperature during the pre- and post-anthesis periods on pollen viability, pollen germination in a medium, pollen germination on the stigma, pollen tube growth and pod set in a heat-tolerant (ICCV 92944) and a heat-sensitive (ICC 5912) genotype was studied. The plants were evaluated under heat stress and non-heat stress conditions in controlled environments. High temperature stress (29/16°C to 40/25°C) was gradually applied at flowering to study pollen viability and stigma receptivity including flower production, pod set and seed number. This was compared with a non-stress treatment (27/16°C). The high temperatures reduced pod set by reducing pollen viability and pollen production per flower. The ICCV 92944 pollen was viable at 35/20°C (41% fertile) and at 40/25°C (13% fertile), whereas ICC 5912 pollen was completely sterile at 35/20°C with no in vitro germination and no germination on the stigma. However, the stigma of ICC 5912 remained receptive at 35/20°C and non-stressed pollen (27/16°C) germinated on it during reciprocal crossing. These data indicate that pollen grains were more sensitive to high temperature than the stigma in chickpea. High temperature also reduced pollen production per flower, % pollen germination, pod set and seed number.

scholarly journals In VitroPollen Viability and Pollen Germination in Cherry Laurel (Prunus laurocerasusL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Melekber Sulusoglu ◽  
Aysun Cavusoglu
Keyword(s):  
Pollen Germination ◽  
Culture Media ◽  
Pollen Viability ◽  
Second Step ◽  
Triphenyl Tetrazolium Chloride ◽  
Tetrazolium Chloride ◽  
Germinated Pollen ◽  
Cherry Laurel ◽  
Better Than

Pollen quality is important for growers and breeders. This study was carried out to determinein vitropollen viability and pollen germination in seven genotypes of cherry laurel (Prunus laurocerasusL.). Two pollen viability tests, TTC (2,3,5-triphenyl tetrazolium chloride) and IKI (iodine potassium iodide), were used. Pollen traits of genotypes were studied using anin vitromedium containing 0%, 5%, 10%, 15%, and 20% sucrose to determine the best sucrose concentrations for germination. In the second step, the germinated pollen was counted 1, 4, 6, 10, 12, 24, and 48 hours later until there was no further germination. The viability rates were different according to genotypes and tests used. The IKI and TTC staining tests and pollen germination had low correlation (r2= 0.0614 andr2= 0.0015, resp.). Painted pollen rate was higher and pollen was well-stained with IKI test and pollen viability estimated with TTC staining test was better than that estimated with the IKI staining test. 15% sucrose gave the best germination rates in most of the genotypes. Pollen germination rates were recorded periodically from one hour to 48 hours in 15% sucrose and the results showed that pollen germination rates increased after 6 hours of being placed in culture media.

Viability and in Vitro Germination of Johnsongrass (Sorghum Halepense) Pollen

2007 ◽  
Vol 21 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Ian C. Burke ◽  
John W. Wilcut ◽  
Nina S. Allen
Keyword(s):  
Suspension Culture ◽  
Boric Acid ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Calcium Nitrate ◽  
Medium Composition ◽  
In Vitro Tests ◽  
In Vitro Germination ◽  
Agar Culture

A high proportion of viable pollen grains must germinate to study the physiology of pollen growth to reduce the confounding effects of environmental influences on pollen germination. The objectives of this study were to evaluate the nuclear state and develop a suitable medium and culture method for in vitro germination of johnsongrass pollen. Johnsongrass pollen was trinucleate, and in vitro tests for pollen viability using Alexander's stain and a fluorochromatic reaction method (FCR) indicated johnsongrass pollen was viable (92.6 to 98.4%). A factorial treatment arrangement of four concentrations of sucrose, two concentrations of boric acid, and two concentrations of calcium nitrate were used to determine the optimum pollen-germination medium composition in suspension culture, agar culture, and cellophane membrane culture. Germination was highest in a suspension culture with a medium containing 0.3 M sucrose, 2.4 mM boric acid, and 3 mM calcium nitrate. Pollen germination using this medium was 78.9% when anthers were harvested just before anthesis.

scholarly journals Colchicine did not affect the viability of induced 2n pollen in Populus tomentosa

Silva Fennica ◽  
2019 ◽  
Vol 53 (2) ◽  
Author(s):  
Yan Liu ◽  
Yuan Zhang ◽  
Qing Zhou ◽  
Jian Wu ◽  
Pingdong Zhang
Keyword(s):  
Pollen Germination ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Populus Tomentosa ◽  
2N Pollen ◽  
Significant Difference ◽  
Diploid Pollen ◽  
Germination Experiments ◽  
Diploid Gametes

Colchicine is widely used as a mutagen to induce production of diploid gametes in plants. However, whether colchicine affects induced pollen viability remains unclear. To clarify whether colchicine affected the viability of induced pollen, we induced production of diploid pollen by colchicine, followed by pollen germination and crossing induced pollen with normal gametes to produce triploid in Carrière. The results showed that the predominant meiotic stages and the number of colchicine injections had significant effects on the occurrence rates of induced 2n pollen. When the colchicine injection was given at diakinesis, a significant decrease in the pollen production per bud was observed ( < 0.001). The morphology of the colchicine-induced 2n pollen was similar to that of the natural 2n pollen in its ectexine structure. The pollen germination experiments revealed that there was also no significant difference in germination rates between the induced diploid pollen and natural 2n pollen grains, and 68 triploids were created by crossing colchicine-induced pollen. Our findings revealed that colchicine injection could induce to produce 2n pollen and will not lead to dysfunction of induced diploid pollen.in vitroPopulus tomentosapP. tomentosa

scholarly journals Technique for In Vitro Pollen Germination and Short-term Pollen Storage in Caladium

HortScience ◽  
2004 ◽  
Vol 39 (2) ◽  
pp. 365-367 ◽  
Author(s):  
Zhanao Deng ◽  
Brent K. Harbaugh
Keyword(s):  
Pollen Germination ◽  
Large Scale ◽  
Pollen Viability ◽  
Storage Conditions ◽  
In Vitro Germination ◽  
Short Term ◽  
Pollen Storage ◽  
Breeding Programs ◽  
Sucrose Level

The sporadic nature of inflorescence production and flower protogyny in caladium (Caladium ×hortulanum Birdsey) makes it desirable to store pollen and to rapidly assess its viability for cross-pollinations in breeding programs. This study was conducted to develop a procedure to determine caladium pollen viability and to use that procedure to evaluate the effect of short-term storage conditions on pollen viability. The sucrose level in the culture medium was found to have a significant impact on the in vitro germination of caladium pollen; a concentration of 6.8% was determined to be optimal for pollen germination. Caladium pollen lost viability within 1 day under room (24 °C) or freezing (-20 °C) temperatures, but could be stored at 4 °C for 2 to 4 days. Pollen stored at 4 °C produced successful pollinations. Data obtained from large-scale greenhouse pollinations supported use of this in vitro germination assay as a convenient way to evaluate caladium pollen viability (and fertility).

scholarly journals In vitro germination and viability of pea pollen grains after application of organic nano-fertilizers

2017 ◽  
Vol 32 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Natalia Georgieva ◽  
Ivelina Nikolova ◽  
Valentin Kosev ◽  
Yordanka Naydenova
Keyword(s):  
Pollen Tube ◽  
Pollen Germination ◽  
Culture Media ◽  
Pollen Viability ◽  
Pollen Grains ◽  
Pollen Grain ◽  
In Vitro Germination ◽  
Pisum Sativum L ◽  
Pollen Tube Elongation

The objective of this study was to evaluate the influence of two organic nanofertilizers, Lithovit and Nagro, on in vitro germination, pollen tube elongation and pollen grain viability of Pisum sativum L cv. Pleven 4. The effect of their application was high and exceeded data for the untreated control (44.2 and 47.23 % regarding pollen germination and pollen tube elongation, respectively), as well as the effect of the control organic algal fertilizer Biofa (17.5 and 27.9 %, respectively). Pollen grains were inoculated in four culture media. A medium containing 15% sucrose and 1% agar had the most stimulating impact on pea pollen grains. Pollen viability, evaluated by staining with 1% carmine, was within limits of 74.72-87.97%. The highest viability of pollen grains was demonstrated after the application of Nagro organic nano-fertlizer.

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