scholarly journals Changes in peroxidase and polyphenol oxidase activity and transcript levels of related genes in two egyptian bread wheat cultivars (Triticum aestivum L.) affected by gamma irradiation and salinity stress

2019 ◽  
Vol 48 (1) ◽  
pp. 177-186 ◽  
Author(s):  
Amina Abd El-Hamid Aly ◽  
Rabab Waheed Maraei ◽  
Ibrahim Aldrussi
Keyword(s):  
Gamma Irradiation ◽  
Polyphenol Oxidase ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Maximal Activity ◽  
Triticum Aestivum L ◽  
Wheat Cultivars ◽  
Genetic Response ◽  
Polyphenol Oxidase Activity ◽  
Enzyme Stimulation

The effect of gamma irradiation (100, 200, and 300 Gy) and NaCl (60 and 120 mM) on peroxidase (POD) and polyphenol oxidase (PPO) activities in Sids-1 and Sakha-93 wheat cultivars were investigated. POD and PPO activities increased significantly with increasing NaCl concentrations, and the maximal activity increased at 120 mM NaCl, while PPO activity was higher than POD at the same concentration of NaCl in both the two cultivars. POD and PPO isozymes profile revealed a total of six bands, whereas all of them are variable and exposed the effect of gamma ray and sodium chloride with different genetic response of the two wheat cultivars. The expression level of POD gene in Sakha-93 cultivar was more than in Sids-1. While it was more pronounced at 120 mM NaCl combined with gamma irradiation dose 300 Gy. Gamma ray can be used as a useful tool for gene expression in plants and salt tolerance could be attributed to the constitutive induced antioxidant gene, leading to more efficient enzyme stimulation and protection in wheat.

Effect of gamma irradiation dose on the fabrication of α-elastin nanoparticles by gamma-ray crosslinking

2011 ◽  
Vol 80 (2) ◽  
pp. 142-144 ◽  
Author(s):  
Mari Fujimoto ◽  
Mayuko Takeda ◽  
Kouji Okamoto ◽  
Masakazu Furuta
Keyword(s):  
Gamma Irradiation ◽  
Irradiation Dose ◽  
Gamma Ray

scholarly journals Evaluasi Keragaman Genetik Mutan Harapan Generasi MV1 Jeruk Keprok SoE (Citrus reticulata Blanco) Berdasarkan Penanda Morfologi dan ISSR

2016 ◽  
Vol 7 (2) ◽  
pp. 102
Author(s):  
Indriati Husain ◽  
Agus Purwito ◽  
Ali Husni ◽  
Kikin H. Mutaqin ◽  
Slamet Susanto
Keyword(s):  
Genetic Diversity ◽  
Gamma Irradiation ◽  
Genetic Distance ◽  
Embryogenic Callus ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Morphological Diversity ◽  
Issr Markers ◽  
Citrus Reticulata ◽  
Gamma Ray Irradiation

<p class="Abstrak"><em>ABSTRACT</em><em></em></p><p><em>Mandarin’s SoE is national <del cite="mailto:Windows%207" datetime="2016-10-31T15:54"></del>variety originated <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins>from Mount of Mutis, Sub District of SoE, of Timur Tengah Selatan (TTS) District, East Nusa Tenggara (NTT). The genetic diversity of citrus can be induced by gamma ray irradiation on embryogenic callus cells thus producing new mutants. <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del>Genetic diversity detection can be <ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>based on morphological and ISSR markers. The aim of this research was <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>to obtain information on the genetic diversity <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>on putative mutants<ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins> mandarin SoE induced by <ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>gamma ray irradiation <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del>based on morphology and markers ISSR. ISSR markers used are ISSR 1, 4, 6 and 8. Analysis of morphological diversity produced <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>a dendrogram with the level of similarity between individuals each irradiation dose 83-95% with <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>5-17% genetic distance. Dendrogram analysis based on the genetic diversity ISSR markers showed high levels of 51-100% similarity and genetic distance 0-49%. Individuals<del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del> samples obtained from<del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins> gamma irradiation, based <ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins>both morphological and ISSR markers, was different from individual's genetic make up before irradiation.</em></p><p class="Abstrak"><em>Keywords</em><em>:</em><em> cluster, </em><em>gamma ray</em><em>, genetic distance, genetic diversity</em><em>s, </em><em>similarity</em></p><p class="Abstrak"> </p><p class="Abstrak">ABSTRAK</p><p>Jeruk keprok SoE adalah jeruk varietas unggul nasional yang berasal dari Pegunungan Mutis, Kecamatan SoE, Kabupaten Timur Tengah Selatan (TTS), Provinsi Nusa Tenggara Timur (NTT). Keragaman genetik jeruk ini dapat diinduksi dengan iradiasi sinar gamma pada sel-sel kalus embriogenik untuk menghasilkan mutan yang solid. Deteksi keragaman genetik yang terbentuk dapat dilakukan secara morfologi maupun molekuler dengan marka ISSR. Tujuan Penelitian ini adalah untuk mendapatkan informasi mengenai keragaman genetik yang terjadi pada mutan harapan jeruk keprok SoE hasil iradiasi sinar gamma berdasarkan morfologi dan marka ISSR. Marka ISSR yang digunakan adalah ISSR 1, 4, 6 dan 8 pada beberapa mutan harapan jeruk keprok SoE. Analisis keragaman secara morfologi menghasilkan dendrogram dengan tingkat kemiripan antar individu masing-masing dosis iradiasi 83-95% dengan jarak genetik 5-17%. Dendrogram analisis keragaman genetik berdasar marka ISSR memperlihatkan tingkat kemiripan 51-100% dan jarak genetik 0-49%. Individu-individu sampel yang diuji hasil iradiasi gamma, baik secara morfologi dan marka ISSR, telah memiliki susunan genetik yang berbeda dari susunan genetik individu sebelum diiradiasi.</p><p>Kata kunci: grup, jarak genetik, kemiripan, keragaman, sinar gamma</p>

scholarly journals Effects of gamma irradiation and salt stress on amino acids and protein fractions of two egyptian bread wheat (Triticum aestivum L.) cultivars

2019 ◽  
Vol 48 (4) ◽  
pp. 1175-1184
Author(s):  
Amina A Aly ◽  
Rabab W Maraei ◽  
Mokhtar Baraket
Keyword(s):  
Amino Acids ◽  
Gamma Rays ◽  
Irradiation Dose ◽  
Triticum Aestivum L ◽  
Protein Fractions ◽  
Wheat Cultivars ◽  
Protein Patterns ◽  
Maximum Increase ◽  
Wheat Grains ◽  
Amino Acids Composition

Effects of gamma rays (0.0, 100, 200 and 300 Gy) and NaCl (0.0, 60 and 120 mM) on the amino acids composition, protein fractions and electrophoretic protein patterns of two Egyptian wheat cultivars (Sids-1 and Sakha-93) were investigated. The results showed that content of amino acids increased by salinity and irradiation doses compared to the control. Concerning, protein fractions of albumin + globulin percentage increased by increasing salinity only and reached the maximum increase by treatment of 120 mM NaCl in the two cultivars. But for glutenin, the highest increase was observed in treatment 100 Gy + 60 mM NaCl. The changes in protein patterns profile were found to be dependent on irradiation dose and salinity level in wheat grains. Total number of protein bands decreased from 20 bands (untreated plants) to 13 bands (irradiated plants) due to treatment of 200 Gy and 60 mM NaCl in Sids-1 cultivar.

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