Effect of gamma irradiation dose on the fabrication of α-elastin nanoparticles by gamma-ray crosslinking

2011 ◽  
Vol 80 (2) ◽  
pp. 142-144 ◽  
Author(s):  
Mari Fujimoto ◽  
Mayuko Takeda ◽  
Kouji Okamoto ◽  
Masakazu Furuta
Keyword(s):  
Gamma Irradiation ◽  
Irradiation Dose ◽  
Gamma Ray

scholarly journals Evaluasi Keragaman Genetik Mutan Harapan Generasi MV1 Jeruk Keprok SoE (Citrus reticulata Blanco) Berdasarkan Penanda Morfologi dan ISSR

2016 ◽  
Vol 7 (2) ◽  
pp. 102
Author(s):  
Indriati Husain ◽  
Agus Purwito ◽  
Ali Husni ◽  
Kikin H. Mutaqin ◽  
Slamet Susanto
Keyword(s):  
Genetic Diversity ◽  
Gamma Irradiation ◽  
Genetic Distance ◽  
Embryogenic Callus ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Morphological Diversity ◽  
Issr Markers ◽  
Citrus Reticulata ◽  
Gamma Ray Irradiation

<p class="Abstrak"><em>ABSTRACT</em><em></em></p><p><em>Mandarin’s SoE is national <del cite="mailto:Windows%207" datetime="2016-10-31T15:54"></del>variety originated <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins>from Mount of Mutis, Sub District of SoE, of Timur Tengah Selatan (TTS) District, East Nusa Tenggara (NTT). The genetic diversity of citrus can be induced by gamma ray irradiation on embryogenic callus cells thus producing new mutants. <del cite="mailto:Windows%207" datetime="2016-10-31T15:55"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><ins cite="mailto:Windows%207" datetime="2016-10-31T15:55"></ins><del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del>Genetic diversity detection can be <ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>based on morphological and ISSR markers. The aim of this research was <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>to obtain information on the genetic diversity <del cite="mailto:Windows%207" datetime="2016-10-31T15:56"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins>on putative mutants<ins cite="mailto:Windows%207" datetime="2016-10-31T15:56"></ins> mandarin SoE induced by <ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>gamma ray irradiation <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del>based on morphology and markers ISSR. ISSR markers used are ISSR 1, 4, 6 and 8. Analysis of morphological diversity produced <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>a dendrogram with the level of similarity between individuals each irradiation dose 83-95% with <del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:57"></ins>5-17% genetic distance. Dendrogram analysis based on the genetic diversity ISSR markers showed high levels of 51-100% similarity and genetic distance 0-49%. Individuals<del cite="mailto:Windows%207" datetime="2016-10-31T15:57"></del> samples obtained from<del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><del cite="mailto:Windows%207" datetime="2016-10-31T15:58"></del><ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins> gamma irradiation, based <ins cite="mailto:Windows%207" datetime="2016-10-31T15:58"></ins>both morphological and ISSR markers, was different from individual's genetic make up before irradiation.</em></p><p class="Abstrak"><em>Keywords</em><em>:</em><em> cluster, </em><em>gamma ray</em><em>, genetic distance, genetic diversity</em><em>s, </em><em>similarity</em></p><p class="Abstrak"> </p><p class="Abstrak">ABSTRAK</p><p>Jeruk keprok SoE adalah jeruk varietas unggul nasional yang berasal dari Pegunungan Mutis, Kecamatan SoE, Kabupaten Timur Tengah Selatan (TTS), Provinsi Nusa Tenggara Timur (NTT). Keragaman genetik jeruk ini dapat diinduksi dengan iradiasi sinar gamma pada sel-sel kalus embriogenik untuk menghasilkan mutan yang solid. Deteksi keragaman genetik yang terbentuk dapat dilakukan secara morfologi maupun molekuler dengan marka ISSR. Tujuan Penelitian ini adalah untuk mendapatkan informasi mengenai keragaman genetik yang terjadi pada mutan harapan jeruk keprok SoE hasil iradiasi sinar gamma berdasarkan morfologi dan marka ISSR. Marka ISSR yang digunakan adalah ISSR 1, 4, 6 dan 8 pada beberapa mutan harapan jeruk keprok SoE. Analisis keragaman secara morfologi menghasilkan dendrogram dengan tingkat kemiripan antar individu masing-masing dosis iradiasi 83-95% dengan jarak genetik 5-17%. Dendrogram analisis keragaman genetik berdasar marka ISSR memperlihatkan tingkat kemiripan 51-100% dan jarak genetik 0-49%. Individu-individu sampel yang diuji hasil iradiasi gamma, baik secara morfologi dan marka ISSR, telah memiliki susunan genetik yang berbeda dari susunan genetik individu sebelum diiradiasi.</p><p>Kata kunci: grup, jarak genetik, kemiripan, keragaman, sinar gamma</p>

scholarly journals Changes in peroxidase and polyphenol oxidase activity and transcript levels of related genes in two egyptian bread wheat cultivars (Triticum aestivum L.) affected by gamma irradiation and salinity stress

2019 ◽  
Vol 48 (1) ◽  
pp. 177-186 ◽  
Author(s):  
Amina Abd El-Hamid Aly ◽  
Rabab Waheed Maraei ◽  
Ibrahim Aldrussi
Keyword(s):  
Gamma Irradiation ◽  
Polyphenol Oxidase ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Maximal Activity ◽  
Triticum Aestivum L ◽  
Wheat Cultivars ◽  
Genetic Response ◽  
Polyphenol Oxidase Activity ◽  
Enzyme Stimulation

The effect of gamma irradiation (100, 200, and 300 Gy) and NaCl (60 and 120 mM) on peroxidase (POD) and polyphenol oxidase (PPO) activities in Sids-1 and Sakha-93 wheat cultivars were investigated. POD and PPO activities increased significantly with increasing NaCl concentrations, and the maximal activity increased at 120 mM NaCl, while PPO activity was higher than POD at the same concentration of NaCl in both the two cultivars. POD and PPO isozymes profile revealed a total of six bands, whereas all of them are variable and exposed the effect of gamma ray and sodium chloride with different genetic response of the two wheat cultivars. The expression level of POD gene in Sakha-93 cultivar was more than in Sids-1. While it was more pronounced at 120 mM NaCl combined with gamma irradiation dose 300 Gy. Gamma ray can be used as a useful tool for gene expression in plants and salt tolerance could be attributed to the constitutive induced antioxidant gene, leading to more efficient enzyme stimulation and protection in wheat.

Effects of Gamma Irradiation on Optical Properties of Colloidal Nanocrystals

2007 ◽  
Vol 1038 ◽  
Author(s):  
Nathan J Withers ◽  
Krishnaprasad Sankar ◽  
Brian A. Akins ◽  
Tosifa A. Memon ◽  
Jiangjiang Gu ◽  
...  
Keyword(s):  
Optical Properties ◽  
Gamma Irradiation ◽  
Irradiation Dose ◽  
Nanocrystalline Material ◽  
Light Output ◽  
Colloidal Nanocrystals ◽  
Photoluminescence Intensity ◽  
Photoluminescence Properties ◽  
Material Systems ◽  
Dose Radiation

AbstractThe effects of 137Cs gamma irradiation on photoluminescence properties, such as spectra, light output, and lifetime, of several types of colloidal nanocrystals have been investigated. Irradiation-induced damage testing was performed on CdSe/ZnS, LaF3:Eu, LaF3:Ce, ZnO, and PbI2 nanocrystals synthesized on a Schlenk line using appropriate solvents and precursors. Optical degradation of the nanocrystals was evaluated based on the measured dependence of their photoluminescence intensity on the irradiation dose. Radiation hardness varies significantly between various nanocrystalline material systems.

Synthesis, Quantum Chemical Calculations, Molecular Docking and Studying the Effect of High Energetic Gamma Irradiation on Cu(I, II), Zn(II) and Cd(II) Complexes with their Antibacterial Activity.

2021 ◽  
Author(s):  
Hussein Elganzory
Keyword(s):  
Molecular Docking ◽  
Gamma Irradiation ◽  
Density Functional ◽  
Topoisomerase Ii ◽  
Gamma Ray ◽  
Inhibitory Effect ◽  
Molar Conductance ◽  
Basis Set ◽  
Conformational Structure ◽  
Before And After

Abstract New complexes of Cu(I,II), Zn(II) and Cd(II) of thiosemicarbazide ligand 1-(p-(methylanilinocetyl-4-phenyl-thiosemicarbazide)(H2LB) have been prepared and characterized by 1HNMR, Mass spectra, FT-IR, elemental analyses, molar conductance, UV-visible spectra, magnetic susceptibility measurements, thermogravimetric analysis (TGA/DTG) and X-ray diffraction pattern before and after irradiation. The results confirmed that gamma ray enhanced the stability of irradiated compounds as compared to non-irradiated compounds. XRD patterns proved that increasing the crystallinity of the samples and the particles in nano range after gamma irradiation. The obtained data indicated that the Cu(I) and Cd(II) ions coordinated to the ligand through the (C = O), N(2)H and (C = S), the ligand behaves as neutral tridentate. While in complexes Cu(II) and Zn(II)complexes (B2 and B3) the ligand behave as neutral tetradentate and coordination take place via (C = O) and two N(2)H. These studies revealed that, two kinds of stereochemical geometries; Cu(II) and Zn(II) complexes were predicted to be octahedral, Cu(I) and Cd(II)complexes were found to be tetrahedral. The theoretical conformational structure analyses were performed using density functional theory for ligand and complexes at B3LYP functional with 6-31G(++)d,p basis set for ligand and LANL2DZ basis set for complexes. The ligand and its metal complexes have been tested for their inhibitory effect on the growth of bacteria against gram-positive (Streptococcus pyogenes) and gram-negative (Escherichia coli). Results suggested that in case of 1µg/ml and 5µg/ml for Cu(II) and Zn(II) complexes have higher activity than other complexes. The chelation could facilitate the ability to cross the cell membrane of E. coli and can be explained by Tweedy’s chelation theory. Molecular docking investigation proved that; the Zn(II) complex had interesting interactions with active site amino acids of topoisomerase II DNA gyrase enzymes (code: 2XCT).

Characterization of a family of gamma-ray-induced CHO mutants demonstrates that the ldlA locus is diploid and encodes the low-density lipoprotein receptor

1986 ◽  
Vol 6 (9) ◽  
pp. 3268-3277
Author(s):  
R D Sege ◽  
K F Kozarsky ◽  
M Krieger
Keyword(s):  
Gamma Irradiation ◽  
Cho Cells ◽  
Gamma Ray ◽  
Receptor Gene ◽  
Low Density Lipoprotein ◽  
Chinese Hamster ◽  
Ldl Receptor ◽  
Density Lipoprotein ◽  
Low Density ◽  
Ldl Receptors

The ldlA locus is one of four Chinese hamster ovary (CHO) cell loci which are known to be required for the synthesis of functional low-density lipoprotein (LDL) receptors. Previous studies have suggested that the ldlA locus is diploid and encodes the LDL receptor. To confirm this assignment, we have isolated a partial genomic clone of the Chinese hamster LDL receptor gene and used this and other nucleic acid and antibody probes to study a family of ldlA mutants isolated after gamma-irradiation. Our analysis suggests that there are two LDL receptor alleles in wild-type CHO cells. Each of the three mutants isolated after gamma-irradiation had detectable deletions affecting one of the two LDL receptor alleles. One of the mutants also had a disruption of the remaining allele, resulting in the synthesis of an abnormal receptor precursor which was not subject to Golgi-associated posttranslational glycoprotein processing. The correlation of changes in the expression, structure, and function of LDL receptors with deletions in the LDL receptor genes in these mutants directly demonstrated that the ldlA locus in CHO cells is diploid and encodes the LDL receptor. In addition, our analysis suggests that CHO cells in culture may contain a partial LDL receptor pseudogene.

scholarly journals X-ray diffraction and thermodynamics kinetics of SiB6 under gamma irradiation dose

Silicon ◽  
2018 ◽  
Vol 11 (5) ◽  
pp. 2499-2504 ◽  
Author(s):  
M. N. Mirzayev ◽  
S. H. Jabarov ◽  
E. B. Asgerov ◽  
R. N. Mehdiyeva ◽  
T. T. Thabethe ◽  
...  
Keyword(s):  
Gamma Irradiation ◽  
Irradiation Dose ◽  
X Ray Diffraction ◽  
X Ray ◽  
Kinetics Of

scholarly journals SYNTHESIS OF POLYVINYL ALCOHOL-CHITOSAN HYDROGEL AND STUDY OF ITS SWELLING AND ANTIBACTERIAL PROPERTIES

2013 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Thamrin Wikanta ◽  
Mr Erizal ◽  
Mr Tjahyono ◽  
Mr Sugiyono
Keyword(s):  
Polyvinyl Alcohol ◽  
Irradiation Dose ◽  
Gamma Ray ◽  
Antibacterial Properties ◽  
Positive Control ◽  
Negative Control ◽  
Initial Time ◽  
Water Evaporation ◽  
Combination Technique ◽  
Pva Hydrogel

The aim of this research was to synthesize a hydrogel for wound dressing by mixing of polyvinyl alcohol (PVA) and chitosan (CTS) and processed by combination technique of freezing-thawing and irradiation by gamma ray, and to study of its properties. PVA aqueous solution 10% (w/v) was mixed with 2% (w/v) chitosan (CTS) solution and homogenized. The PVA-CTS mixture was processed by freezing-thawing up to 3 cycles, and then irradiated by gamma rays at the doseranged of 20-50 kGy  (dose rate was 10 kGy/hour). Result showed that PVA-CTS hydrogel with the gel fraction of 83%, 87%, 90%, and 83% were obtained at the irradiation dose of 20 kGy, 30 kGy, 40 kGy, and 50 kGy, respectively. Increasing of irradiation dose caused increasing of water absorption of hydrogel, i.e. 1.700 %, 1.715 %, 1.913 %, and 2.036 %, respectively, and the hydrogel reached the equilibrium in 25 hours. The hydrogel showed very slow water evaporation rate (~ 2%) at the initial time (1 hour) and then increased very fast (up ~50 %) at 24 h, i.e. 43%, 39.13%, 44%, and 53%, respectively. The elongation at break of hydrogels were obtained 245%, 322%, 322%, and 205% with the maximum value were obtained at irradiation dose ranged of 30-40 kGy. The presence of chitosan in the PVA hydrogel made it having higher antibacterial properties with the inhibitionzone value of 8 mm at irradiation dose of 30-40 kGy compared to PVA hydrogel as a negative control (6 mm) and to chloramphenicol as a positive control (8 mm).

scholarly journals Study on the immuno stimulation of radiation degraded β-glucan in swiss mice

2015 ◽  
Vol 5 (4) ◽  
pp. 9-15
Author(s):  
Thanh Long Nguyen ◽  
Quang Luan Le
Keyword(s):  
Cell Wall ◽  
Immune System ◽  
Gamma Irradiation ◽  
Gamma Rays ◽  
Irradiation Dose ◽  
Functional Foods ◽  
Water Soluble ◽  
Molecular Weights ◽  
Swiss Mice ◽  
Stimulation Of

The mixtures β-glucan extracted from the yeast cell wall were irradiated under gamma rays from a Co-60 source at doses of 100, 200 and 300 kGy in order to prepare water-soluble β-glucan. Yields of the water soluble β-glucan produced are 25.9, 49.1, 66.71%, and their molecular weights (Mw) are 30.5, 24.9 and 10.8 kDa, respectively. There are no any new peak in the IR spectra of the irradiated β-glucan samples, but the intensity ratio between the peaks at wavenumber of 1156 cm-1 (assigned to C-O-C bond) and of 1040 cm-1 (assigned to C-C bond) in glycosidic linkages was reduced with irradiation dose. These results revealed that gamma irradiation did not cause any change in the β-glucan structure except the scissions of glycosidic linkages. In this study, immuno stimulation of the irradiated b-glucan was also investigated for the Swiss mice. After 28 days supplying with the irradiated b-glucan, not only cellular indexes (white blood cell, neutrophils and lymphocytes counts), but also humoral  immunity indexes (IgA and IgM) of the mice significantly increased and the highest effects was obtained for the mice supplied with the oligoβ-glucan prepared by gamma irradiation at 200 kGy. Thus, the water soluble oligoβ-glucan with Mw ~ 24.9 kDa prepared by gamma radiation much stimulated the natural immune system (non-specific immunity) in mice including both the cellular and humoral immunities. Particularly, the irradiated b-glucan is a very promising product for preparation of functional foods aiming at cancer prevention.

The Behavior of Actinide Containing Glasses During Gammirar Adiation in a Saturated Tuff Environment

1984 ◽  
Vol 44 ◽  
Author(s):  
John K. Bates ◽  
Virginia M. Oversby
Keyword(s):  
Gamma Irradiation ◽  
Radiation Field ◽  
Irradiation Dose ◽  
Dynamic Balance ◽  
Dynamic Interaction ◽  
Solution Ph ◽  
Dose Rates

AbstractThe influence of gamma irradiation on the reaction of actinide doped SRL 165 and PNL 76–68 glasses in a saturated tuff environment has been studied in a series of tests lasting up to 56 days. The reaction, and subsequent actinide release, of both glasses depends on the dynamic interaction between radiolysis effects which cause the solution pH to become more acidic and glass reaction which drives the pH more basic. The use of large gamma irradiation dose rates to accelerate reactions that would occur in an actual repository radiation field may affect this dynamic balance by unduly influencing the mechanism of the glass-water reaction. Comparisons are made between the present results and data obtained by reacting the same or similar glasses using MCC-1 and NNWSI rock cup procedures.

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