Artificial cultivation of a novel wild strain of Lepista sordida (Sowerby) Pat from south west China

Cultivation of Ganoderma lucidum (Curtis) P. Karst, a medicinal mushroom known for antioxidant, antitumor, anti-inflammatory, and immunomodulatory activities is not practiced in Nigeria. Tree species used for cultivation in Asia, America and Europe are not available in Nigeria. The present study investigated indigenous hardwoods and their supplementation with rice and wheat bran as substrates for its cultivation. Six hardwoods (Mansonia altissima (A Chev.) A Chev., Avecennia germinans (L.) L, Lophira alata Banks ex Gaertn., Triplochiton scleoxylon K. Schum, Uapaca guineensis Mull. Arg, Nauclea diderrichii (De Wild. & Th. Dur.) Merrill) were investigated as potential growth substrates and potentially improve biological efficiency. The mushroom was collected from the wild and identification confirmed by amplifying the ribosomal DNA-ITS fragment with ITS1 and ITS4 primers. Tissue culture of the mushroom collected from the wild was initiated successfully with modified malt extract agar and grain spawn developed from it. The substrates were incubated after inoculation with grain spawn at room temperature for 30-60 days. Substrates were given cold treatment for 7days to induce fructification which was achieved with 9-10h/day regime and daily watering. There was full mycelial ramification of all substrates by the mushroom. Fruit bodies were harvested from all substrates but Lophira alata. The highest yield (308.76±5.81g/kg) was recorded with Mansonia altissima (Biological Efficiency-31.42±4.55%) and the least yield (31.45±5.44g/kg) was recorded in Nauclea diderrichii (B.E- 5.25±0.58%). The substrates with wheat bran performed significantly better than rice bran supplemented substrates. Commercial cultivation of native G. lucidum in Nigeria is possible with local agricultural wastes.

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Isolation of Ganoderma lucidum (Curtis) P. Karst. From the Wild in Lagos through Tissue Culture Techniques and Cultivation on Sawdust of Six Nigerian Hardwoods

Journal of Applied Sciences and Environmental Management ◽

10.4314/jasem.v25i7.32 ◽

2021 ◽

Vol 25 (7) ◽

pp. 1329-1335

Author(s):

E.M. Adongbede ◽

A.F. Atoyebi

Keyword(s):

Tissue Culture ◽

Wheat Bran ◽

Ganoderma Lucidum ◽

Cold Treatment ◽

Biological Efficiency ◽

Malt Extract ◽

Potential Growth ◽

Culture Techniques ◽

Commercial Cultivation ◽

Nauclea Diderrichii

Cultivation of Ganoderma lucidum (Curtis) P. Karst, a medicinal mushroom known for antioxidant, antitumor, anti-inflammatory, and immunomodulatory activities is not practiced in Nigeria. Tree species used for cultivation in Asia, America and Europe are not available in Nigeria. The present study investigated indigenous hardwoods and their supplementation with rice and wheat bran as substrates for its cultivation. Six hardwoods (Mansonia altissima (A Chev.) A Chev., Avecennia germinans (L.) L, Lophira alata Banks ex Gaertn., Triplochiton scleoxylon K. Schum, Uapaca guineensis Mull. Arg, Nauclea diderrichii (De Wild. & Th. Dur.) Merrill) were investigated as potential growth substrates and potentially improve biological efficiency. The mushroom was collected from the wild and identification confirmed by amplifying the ribosomal DNA-ITS fragment with ITS1 and ITS4 primers. Tissue culture of the mushroom collected from the wild was initiated successfully with modified malt extract agar and grain spawn developed from it. The substrates were incubated after inoculation with grain spawn at room temperature for 30-60 days. Substrates were given cold treatment for 7days to induce fructification which was achieved with 9-10h/day regime and daily watering. There was full mycelial ramification of all substrates by the mushroom. Fruit bodies were harvested from all substrates but Lophira alata. The highest yield (308.76±5.81g/kg) was recorded with Mansonia altissima (Biological Efficiency-31.42±4.55%) and the least yield (31.45±5.44g/kg) was recorded in Nauclea diderrichii (B.E- 5.25±0.58%). The substrates with wheat bran performed significantly better than rice bran supplemented substrates. Commercial cultivation of native G. lucidum in Nigeria is possible with local agricultural wastes.

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Influence of a chemical preparation of triazole50% cs on sowing the winter wheat against rust

International Journal on Integrated Education ◽

10.31149/ijie.v3i9.626 ◽

2020 ◽

Vol 3 (9) ◽

pp. 231-233

Author(s):

AliyevSh.K. ◽

TuychiyevI.U ◽

Karimov N ◽

Umaraliev.M.I

Keyword(s):

Winter Wheat ◽

Field Experiments ◽

Yellow Rust ◽

Biological Efficiency ◽

Chemical Preparation ◽

Seed Growth ◽

Biological Farming ◽

The Given ◽

Farming Efficiency

The article is focused on the data of the carried works on studying biological efficiency of fungi Triazole 50% on sowing the winter wheat against yellow rust as well as on the height, development and fertility of the wheat. On May 5, 2019 from 9 to 10 o’clock under the temperature 21-23 field experiments of Triazol 50% CS manufactured by the firm “Agroximstar” (Uzbekistan) were carried out on winter wheat as a protector of seeds of winter wheat of Pervitsa sort against the disease of yellow rustin the irrigated conditions in an experimental field of the Institute “Istiklal” of Andijan district of Andijan region. The aim of the given research is to study biological-farming efficiency and determination of optimal norms of preparation expenses and to study the influence of fungicide on the height and development as well as on the fertility of the wheat. The received data showed that the preparation Triazole 50% CS effected on the pathogen of yellow rust favorably and besides that it didn’t effect on seed growth and energy of growth negatively.

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Growth and yield impact of oyster mushroom Pleurotus ostreatus (Jacq P. Kumm) cultivated on different agricultural wastes

Journal of Bioscience and Agriculture Research ◽

10.18801/jbar.270121.272 ◽

2021 ◽

Vol 27 (01) ◽

pp. 2225-2233

Author(s):

M. O. Agba ◽

◽

A. A. Markson ◽

J. O. Oni ◽

G. A. Bassey ◽

...

Keyword(s):

Pleurotus Ostreatus ◽

Growth Parameters ◽

Total Yield ◽

Fruit Body ◽

Food Product ◽

Dry Weight ◽

Edible Mushroom ◽

Growth And Yield ◽

Biological Efficiency ◽

Essential Food

Pleurotus ostreatus, an edible mushroom is an essential food product. Recognised as one of the cheapest sources of proteins, their high nutritional and anti-nutritional properties are of immense importance to humans. The present study evaluated the effect of sawdust and dried plantain leaves on the growth and yield of Pleurotus ostreatus. Sawdust and dried plantain leaves were composted into seven substrates; as single substrates and at different combinations. The composted substrates include sawdust 100 % (M0), Dry plantain leaves 100% (M1), Dry plantain leaves + sawdust at the ratio of 1:4 (M2), 2:3 (M3), 3:2 (M4), 1:1 (M5) and 4:1(M6). Growth parameters of interest assessed flush include mycelia running rate (MRR), area of pileus, length of the stipe, the girth of the stipe, fresh weight and dry weight, number of fruit body, total yield and biological efficiency. At the end of the three weeks spawn running period, M0 was overall best in supporting mycelia running rate with a mean MRR of 16.00 cm. M1 substrates produced mushroom with longer and bigger stipes (7.17 cm) at the first flush. A higher number of fruit bodies (82.66), total yield (130.35 g), and biological efficiency (43.45 g) were all seen in M0 substrates. Sawdust at 100 % (M0) proved to be the best substrate for the cultivation of Pleurotus ostreatus.

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Fruit-body production of an ectomycorrhizal fungus in genus Boletus in pure culture

Mycoscience ◽

10.1007/s10267-003-0120-5 ◽

2003 ◽

Vol 44 (4) ◽

pp. 295-300 ◽

Cited By ~ 16

Author(s):

Akira Ohta ◽

Naoya Fujiwara

Keyword(s):

Pure Culture ◽

Fruit Body ◽

Ectomycorrhizal Fungus ◽

Body Production

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Impact of weed management practices on soil biological activity in corn and soybean field crops in Québec (Canada)

Canadian Journal of Soil Science ◽

10.1139/cjss-2020-0023 ◽

2020 ◽

pp. 1-10

Author(s):

Émile Samson-Brais ◽

Marc Lucotte ◽

Matthieu Moingt ◽

Gilles Tremblay ◽

Serge Paquet

Keyword(s):

Biological Activity ◽

Weed Management ◽

Management Practices ◽

Field Experiments ◽

Soil Functions ◽

Soybean Field ◽

Soil Biological Activity ◽

Field Crops ◽

Fda Hydrolysis ◽

Sampling Times

Repeated applications and combination of glyphosate-containing herbicides (GCH) with other herbicides are two weed management practices (WMP) used to compensate for GCH decreasing efficiency impacts in field crops. These practices may have serious impacts on soil functions because GCH affect soil biota and soil biological activity (SBA). Two field experiments, one with corn and one with soybean crops, were conducted during one growing season. SBA indicators, soil respiration (SR) and fluorescein diacetate (FDA) hydrolysis, were measured at two sampling times following six WMP. These WMP included one or two GCH applications (GCH alone or combined with other herbicides), applications of other herbicides only and mechanical weeding. WMP did not affect FDA neither for corn or soybean at either sampling times. In contrast, WMP affected SR in corn fields at both sampling times and SR in soybean field at the first sampling time. Repeating GCH applications and combining different herbicides led to lower SR, suggesting that these practices decreased SBA, whilst one single GCH application presented higher SR, suggesting that this practice stimulated SBA. Our study demonstrates that using GCH in combination with other herbicides or in multiple applications affects SBA in field conditions. Affecting soil functions and carbon cycle do bear serious weed management implications, and the choice of WMP should be taken into consideration to minimize their impacts on SBA for field crops sustainability.

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