scholarly journals Prevalence and molecular detection of infectious laryngotracheitis virus in chickens in selected areas of Bangladesh

2021 ◽  
pp. 113-117
Author(s):  
MZ Ali ◽  
M Giasuddin
Keyword(s):  
Broiler Chickens ◽  
Winter Season ◽  
Viral Disease ◽  
Broiler Chicks ◽  
High Morbidity ◽  
Rt Pcr ◽  
Infectious Laryngotracheitis Virus ◽  
Infectious Laryngotracheitis ◽  
Layer Chickens ◽  
Laryngotracheitis Virus

Infectious laryngotracheitis (ILT) is a viral disease of poultry species caused by infectious laryngotracheitis virus (ILTV) that shows high morbidity and mortality. The present study was under taken for ILTV prevalence in broiler and layer chickens from four different geographical areas including Bogura, Gazipur, Chattogram and Dhaka districts during 2017 to 2018. Total 350 tracheal swabs were collected and were evaluated by real time RT-PCR (rRT-PCR). The overall 5.14% (18/350) ILTV prevalence was found that included 6.5% (13/200) in layer and 3.33% (5/150) in broiler chickens. The prevalence of ILTV was highest (10%) in layer chickens under age below 20 weeks and broiler chicks showed ILTV (1. 42%) infection when they were 7-14 days old. Winter season showed highest 6.6% prevalence whereas 5% and 3% prevalence were noticed at summer and rainy seasons, respectively. Bang. J. Livs. Res. Vol. 27 (1&2), 2020: P. 113-117

scholarly journals Metagenomic Analysis of the Respiratory Microbiome of a Broiler Flock from Hatching to Processing

2021 ◽  
Vol 9 (4) ◽  
pp. 721
Author(s):  
Kelly A. Mulholland ◽  
Monique G. Robinson ◽  
Sharon J. Keeler ◽  
Timothy J. Johnson ◽  
Bonnie W. Weber ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Microbial Interactions ◽  
Metagenomic Sequencing ◽  
Infectious Laryngotracheitis Virus ◽  
Dna And Rna ◽  
Broiler Flock ◽  
Commercial Broiler ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
Respiratory Microbiome

Elucidating the complex microbial interactions in biological environments requires the identification and characterization of not only the bacterial component but also the eukaryotic viruses, bacteriophage, and fungi. In a proof of concept experiment, next generation sequencing approaches, accompanied by the development of novel computational and bioinformatics tools, were utilized to examine the evolution of the microbial ecology of the avian trachea during the growth of a healthy commercial broiler flock. The flock was sampled weekly, beginning at placement and concluding at 49 days, the day before processing. Metagenomic sequencing of DNA and RNA was utilized to examine the bacteria, virus, bacteriophage, and fungal components during flock growth. The utility of using a metagenomic approach to study the avian respiratory virome was confirmed by detecting the dysbiosis in the avian respiratory virome of broiler chickens diagnosed with infection with infectious laryngotracheitis virus. This study provides the first comprehensive analysis of the ecology of the avian respiratory microbiome and demonstrates the feasibility for the use of this approach in future investigations of avian respiratory diseases.

Molecular characterization of infectious laryngotracheitis virus in naturally infected egg layer chickens in a multi-age flock in Brazil

2014 ◽  
Vol 160 (1) ◽  
pp. 241-252 ◽  
Author(s):  
Rodrigo de Macedo Couto ◽  
Ingred Sales Preis ◽  
Juliana Fortes Vilarinho Braga ◽  
Bruno S. A. F. Brasil ◽  
Marcela Gonçalves Drummond ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Infectious Laryngotracheitis Virus ◽  
Infectious Laryngotracheitis ◽  
Layer Chickens ◽  
Laryngotracheitis Virus

scholarly journals Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3203
Author(s):  
Mohamed El-Tholoth ◽  
Huiwen Bai ◽  
Michael G. Mauk ◽  
Eman Anis ◽  
Haim H. Bau
Keyword(s):  
Microfluidic Device ◽  
Clinical Performance ◽  
Lamp Assay ◽  
Viral Disease ◽  
Control Measures ◽  
Infectious Laryngotracheitis Virus ◽  
Molecular Tests ◽  
Infectious Laryngotracheitis ◽  
Financial Burdens ◽  
Laryngotracheitis Virus

Infectious laryngotracheitis (ILT) is a viral disease of chickens’ respiratory system that imposes considerable financial burdens on the chicken industry. Rapid, simple, and specific detection of this virus is crucial to enable proper control measures. Polymerase chain reaction (PCR)-based molecular tests require relatively expensive instruments and skilled personnel, confining their application to centralized laboratories. To enable chicken farms to take timely action and contain the spread of infection, we describe a rapid, simple, semi-quantitative benchtop isothermal amplification (LAMP) assay, and a field-deployable microfluidic device for the diagnosis of ILTV infection in chickens. Our assay performance was compared and favorably agreed with quantitative PCR (qPCR). The sensitivity of our real-time LAMP test is 250 genomic copies/reaction. Clinical performance of our microfluidic device using samples from diseased chickens showed 100% specificity and 100% sensitivity in comparison with benchtop LAMP assay and the gold-standard qPCR. Our method facilitates simple, specific, and rapid molecular ILTV detection in low-resource veterinary diagnostic laboratories and can be used for field molecular diagnosis of suspected ILT cases.

Marked differences in virulence of three Australian field isolates of infectious laryngotracheitis virus in meat and layer chickens

2020 ◽  
Vol 49 (6) ◽  
pp. 600-610
Author(s):  
Shahid Nazir ◽  
Addisu A. Yegoraw ◽  
Richard P. G. Charlesworth ◽  
Sarah Williamson ◽  
Sue Sharpe ◽  
...  
Keyword(s):  
Infectious Laryngotracheitis Virus ◽  
Field Isolates ◽  
Infectious Laryngotracheitis ◽  
Layer Chickens ◽  
Laryngotracheitis Virus

Chicken embryo origin-like strains are responsible for Infectious laryngotracheitis virus outbreaks in Egyptian cross-bred broiler chickens

Virus Genes ◽  
2013 ◽  
Vol 46 (3) ◽  
pp. 423-430 ◽  
Author(s):  
Awad A. Shehata ◽  
Mohammad Y. Halami ◽  
Hesham H. Sultan ◽  
Alaa G. Abd El-Razik ◽  
Thomas W. Vahlenkamp
Keyword(s):  
Chicken Embryo ◽  
Broiler Chickens ◽  
Infectious Laryngotracheitis Virus ◽  
Infectious Laryngotracheitis ◽  
Laryngotracheitis Virus ◽  
Embryo Origin
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