Molecular Detection of Infectious Laryngotracheitis Virus in Chickens with a Microfluidic Chip

Infectious laryngotracheitis (ILT) is a viral disease of chickens’ respiratory system that imposes considerable financial burdens on the chicken industry. Rapid, simple, and specific detection of this virus is crucial to enable proper control measures. Polymerase chain reaction (PCR)-based molecular tests require relatively expensive instruments and skilled personnel, confining their application to centralized laboratories. To enable chicken farms to take timely action and contain the spread of infection, we describe a rapid, simple, semi-quantitative benchtop isothermal amplification (LAMP) assay, and a field-deployable microfluidic device for the diagnosis of ILTV infection in chickens. Our assay performance was compared and favorably agreed with quantitative PCR (qPCR). The sensitivity of our real-time LAMP test is 250 genomic copies/reaction. Clinical performance of our microfluidic device using samples from diseased chickens showed 100% specificity and 100% sensitivity in comparison with benchtop LAMP assay and the gold-standard qPCR. Our method facilitates simple, specific, and rapid molecular ILTV detection in low-resource veterinary diagnostic laboratories and can be used for field molecular diagnosis of suspected ILT cases.

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Prevalence and molecular detection of infectious laryngotracheitis virus in chickens in selected areas of Bangladesh

Bangladesh Journal of Livestock Research ◽

10.3329/bjlr.v27i1.55175 ◽

2021 ◽

pp. 113-117

Author(s):

MZ Ali ◽

M Giasuddin

Keyword(s):

Broiler Chickens ◽

Winter Season ◽

Viral Disease ◽

Broiler Chicks ◽

High Morbidity ◽

Rt Pcr ◽

Infectious Laryngotracheitis Virus ◽

Infectious Laryngotracheitis ◽

Layer Chickens ◽

Laryngotracheitis Virus

Infectious laryngotracheitis (ILT) is a viral disease of poultry species caused by infectious laryngotracheitis virus (ILTV) that shows high morbidity and mortality. The present study was under taken for ILTV prevalence in broiler and layer chickens from four different geographical areas including Bogura, Gazipur, Chattogram and Dhaka districts during 2017 to 2018. Total 350 tracheal swabs were collected and were evaluated by real time RT-PCR (rRT-PCR). The overall 5.14% (18/350) ILTV prevalence was found that included 6.5% (13/200) in layer and 3.33% (5/150) in broiler chickens. The prevalence of ILTV was highest (10%) in layer chickens under age below 20 weeks and broiler chicks showed ILTV (1. 42%) infection when they were 7-14 days old. Winter season showed highest 6.6% prevalence whereas 5% and 3% prevalence were noticed at summer and rainy seasons, respectively. Bang. J. Livs. Res. Vol. 27 (1&2), 2020: P. 113-117

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Laying Hens Affected By Newcastle Virus And Laryngotracheitis Virus Show Rapid Recovery After Treatment With Ivermectin

10.1101/2020.07.29.226027 ◽

2020 ◽

Author(s):

Manolo Fernandez-Díaz ◽

Luis Guevara ◽

Eliana Icochea ◽

Angela Montalván ◽

Doris Villanueva-Pérez ◽

...

Keyword(s):

Egg Production ◽

Standard Treatment ◽

Laying Hens ◽

Infectious Laryngotracheitis Virus ◽

Molecular Tests ◽

Clinical Observations ◽

Remarkable Recovery ◽

Infectious Laryngotracheitis ◽

Laryngotracheitis Virus ◽

The City

SUMMARYThis is an anecdotal observation of an intervention study involving laying hens from a commercial farm in the city of Chincha, Peru, who suffered an outbreak caused by Newcastle disease virus (NDV) and infectious laryngotracheitis virus (ILTV), confirmed by clinical observations, serological and molecular tests (PCR).In addition to receiving standard treatment appropriate to the state of health at the time of the operation, a group of birds were treated with a single dose of ivermectin administered subcutaneously (0.2 mL of a 1% solution equivalent to 200 µg/kg body weight), with a group of control birds not receiving the treatment being reserved.The results showed a remarkable recovery of symptoms after 24 hours of treatment among the birds that received ivermectin. At 4 days after treatment, the birds that received ivermectin showed visibly greater mobility and vivacity, as well as a recovery in egg production. PCR tests after 4 days of treatment with ivermectin were negative for NDV and ILTV.These results are interesting and suggest a possible effect of ivermectin against NDV and ILTV in birds. More controlled studies are needed to confirm this hypothesis.

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Molecular epidemiology of respiratory viruses in commercial chicken flocks in Pakistan from 2014 through to 2016

BMC Veterinary Research ◽

10.1186/s12917-019-2103-6 ◽

2019 ◽

Vol 15 (1) ◽

Cited By ~ 3

Author(s):

Sajid Umar ◽

Angélique Teillaud ◽

Hassan Bin Aslam ◽

Jean-Luc Guerin ◽

Mariette F. Ducatez

Keyword(s):

Respiratory Viruses ◽

Control Measures ◽

Economic Losses ◽

Infectious Laryngotracheitis Virus ◽

Respiratory Problems ◽

Infectious Bronchitis ◽

Commercial Chicken ◽

Infectious Laryngotracheitis ◽

Commercial Poultry ◽

Laryngotracheitis Virus

Abstract Background Viral diseases are a matter of great concern for poultry farmers in Pakistan. Multiple common viral respiratory diseases (CVRDs) cause huge economic losses in the poultry industry. The prevalence of CVRDs in many countries, including Pakistan, is not clearly understood. Results Incidences of 5 chicken respiratory viruses: avian influenza virus (AIV), Newcastle disease virus (NDV/AAVV-1), infectious bronchitis virus (IBV), avian metapneumovirus (aMPV) and infectious laryngotracheitis virus (ILTV) were assessed on commercial Pakistani farms with respiratory problems from 2014 through to 2016. While AIV and AAVV-1 were frequently detected (16 to 17% of farms), IBV and aMPV were rarely detected (in 3 to 5% of farms) and ILTV was not detected. We characterized H9 AIV of the G1 lineage, genotype VII AAVV-1, GI-13 IBV, and type B aMPV strains with very little genetic variability in the 2-year study period. Co-infections with AIV and AAVV-1 were common and wild type AAVV-1 was detected despite the use of vaccines. Control measures to limit the virus burden in chicken flocks are discussed. Conclusions Our data shows that AIV (H9), AAVV-1, IBV and aMPV are prevalent in commercial poultry in Pakistan. Further studies are necessary to assess circulating strains, economic losses caused by infections and coinfections of these pathogens, and the costs and benefits of countermeasures. Furthermore, veterinarians and farmers should be informed of the pathogens circulating in the field and hence advised on the use of vaccines.

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