scholarly journals Role of Light Emitting Diode (LED) Fluorescence Microscopy in the Diagnosis of Smear Negative Pulmonary Tuberculosis.

1970 ◽  
Vol 26 (1) ◽  
pp. 3-6
Author(s):  
Zohra Khatun ◽  
Chandan Kumar Roy ◽  
Tuhin Sultana ◽  
Md Quddusur Rahman ◽  
AN Nashimuddin Ahmed
Keyword(s):  
Fluorescence Microscopy ◽  
Pulmonary Tuberculosis ◽  
Light Emitting Diode ◽  
Light Emitting ◽  
Lowenstein Jensen ◽  
Smear Negative ◽  
Brightfield Microscopy ◽  
Negative Sputum ◽  
A Prospective Study

Light emitting diode (LED) fluorescence microscopy offers well described benefits compared with brightfield microscopy by Ziehl-Neelsen stained sputum, even which are smear negative. We evaluated the diagnostic performance of fluorescence microscopy, using novel light-emitting diode (LED) technology as an alternative to the brightfield microscopy. The objective of this study was the role of LED fluorescence microscopy in diagnosis of smear negative pulmonary tUberculosis. This is a prospective study consisted of 50 smear negative patients, who were clinically suspected cases of pulmonary tuberculosis. All samples were stained by both ZN stain and Auramine stain and as a gold standard all were cultured on Lowenstein-Jensen Media. On evaluation of all sputum samples were found negative by ZN method but by auramine stain 16%, 20%, 20% cases were found positive by conventional fluorescence microscopy (CFM), LED and culture respectively. LED fluorescence microscopy is more useful test to distinguish the smear negative cases. It also provide an effective guideline to make decisions regarding judicious use of antitubercular drug therapy. Keywords: Smear negative Sputum; LED; CFM; Culture; Pulmonary Tuberculosis DOI: http://dx.doi.org/10.3329/bjpath.v26i1.9126 BJPATH 2011; 26(1): 3-6

scholarly journals Usefulness of Light Emitting Diode (LED) fluorescent microscopy as a tool for rapid and effective method for the diagnosis of pulmonary tuberculosis

1970 ◽  
Vol 37 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Z Khatun ◽  
M Kamal ◽  
CK Roy ◽  
T Sultana ◽  
MQ Rahman ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Pulmonary Tuberculosis ◽  
Light Emitting Diode ◽  
Fluorescent Microscopy ◽  
Infectious Agent ◽  
Sputum Smear ◽  
Light Emitting ◽  
Brightfield Microscopy ◽  
The Difference ◽  
A Prospective Study

Background: Tuberculosis remains world's leading cause of death from a single infectious agent. Fluorescence microscopy offers well-described benefits, comparing with brightfield microscopy, for the evaluation sputum smear samples for tuberculosis. We evaluated the diagnostic performance of fluorescence microscopy, using novel Light Emitting Diode (LED) technology as an alternative to the conventional fluorescence microscopy by Auramine stain as well as brightfield microscopy by Ziehl- Neelsen (ZN) stain.Objectives: The objective of the study was to see the usefulness of LED fluorescent microscopy in the diagnosis of pulmonary tuberculosis.Methods: This is a prospective study consisted of 150 sputum samples from the patients of NIDCH, Mohakhali. All samples were stained by auramine and ZN stain at BSMMU and culture was done in Lowenstein-Jensen (L-J) media as gold standard at NTRL, Mohakhali.Results: In this study total 66(44%) out of 150 sputum specimens were positive for Mycobacterium Tuberculosis by culture. Sensitivity and specificity documented for the different modalities were 95.38% and 94.11%, respectively, for the LED assessment; 68.18% and 90.47%, respectively, for the CFM assessment; and 56.06% and 97.61%, respectively, for brightfield microscopy by ZN stain. The difference in their case detection rate was statistically significant (χ2=119.38, p<0.001).Conclusion: Fluorescence Microscopy (FM) is more sensitive than ZN for diagnosis of pulmonary tuberculosis. However, since FM is more sensitive and rapid, using this method (LED) in clinical laboratories with large specimen numbers is recommended. DOI: http://dx.doi.org/10.3329/bmrcb.v37i1.7792 Bangladesh Med Res Counc Bull 2011; 37: 7 -10

scholarly journals Fluorescence microscopy for the diagnosis of smear-negative pulmonary tuberculosis in Ethiopia

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Gemeda Abebe ◽  
Dossegnaw Aragaw ◽  
Mulualem Tadesse
Keyword(s):  
Confidence Interval ◽  
Fluorescence Microscopy ◽  
Pulmonary Tuberculosis ◽  
Diagnostic Performance ◽  
Light Emitting Diode ◽  
Fluorescent Microscopy ◽  
Culture Method ◽  
Light Emitting ◽  
Smear Negative ◽  
Negative Sputum

Background: Despite its low sensitivity, microscopy remains the main method for the diagnosis of pulmonary tuberculosis in most laboratories in Ethiopia. Few studies have evaluated the performance of light-emitting diode fluorescent microscopy (LED-FM) in bleach-concentrated smear-negative sputum specimens.Objective: This study aimed to evaluate the diagnostic performance of LED-FM for smear-negative pulmonary tuberculosis in Ethiopia.Methods: A total of 194 adult patients with a cough lasting for more than two weeks, and who had three direct smear-negative sputum tests for Mycobacterium tuberculosis by Ziehl-Neelsen light microscopy, were included. All direct Ziehl-Neelsen-stained smear-negative sputum samples were cultured and were also visualised by LED-FM. Smears for LED-FM were performed from bleach-concentrated sputum sediment. The diagnostic performance of the LED-FM was compared to the culture method (the reference standard).Results: Of the 194 smear-negative sputum specimens analysed, 28 (14.4%) were culture-positive and 21 (10.8%) were LED-FM-positive for M. tuberculosis. However, only 11 of the 21 (52.4%) LED-FM-positive patients had a confirmed tuberculosis diagnosis by culture. Light-emitting diode fluorescence microscopy (FM) had a sensitivity of 39.3% (95% confidence interval: 21.2–57.4) and specificity of 93.9% (95% confidence interval: 90.4–97.6). Ten LED-FM-positive specimens were culture-negative, and all of these specimens had scanty grading (1–19 bacilli per 40 fields on LED-FM).Conclusion: This study showed that implementation of LED-FM on bleach pre-treated and concentrated sputum can significantly improve the diagnosis of smear-negative pulmonary tuberculosis. However, all scanty grade, positive smears by LED-FM need to be confirmed by reference culture method.

scholarly journals Xpert MTB/Rif assay in the diagnosis of smear-negative pulmonary tuberculosis: a prospective study from Nepal

2019 ◽  
Author(s):  
Priyatam Khadka ◽  
Januka Thapaliya ◽  
Ramesh Bahadur Basnet ◽  
Gokarna Raj Ghimire ◽  
Jyoti Amatya ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Disease Transmission ◽  
Clinical Information ◽  
University Teaching ◽  
Smear Microscopy ◽  
Line Probe Assay ◽  
Smear Negative ◽  
Negative Sputum ◽  
Probe Assay ◽  
A Prospective Study

Abstract Background For improving patient care and abbreviating the disease transmission chain, speedy detection of tuberculosis and its drug-resistance with precision is crucial. Methods We analyzed, pulmonary tuberculosis (PTB) suspected, 360 smear-negative sputum from the patients attending Tribhuvan University Teaching Hospital (TUTH). The patients were selected as per the algorithm of National Tuberculosis Programme(NTP) for Xpert MTB/RIF testing. Participants’ demographic and clinical information were collected using a pre-tested questionnaire. The specimens were collected, processed directly for Xpert MTB/RIF test according to the manufacturer’s protocol. The same samples were stained using Ziehl-Neelsen technique then observed microscopically. Both findings were interpreted; rifampicin-resistant, if obtained, on Xpert testing was confirmed with Line Probe Assay. Result Of 360 smear-negative sputum samples analyzed, 85(23.61%) found positive while 3 of them were rifampicin resistance. The infection was higher in male, i.e. 60(25.3%) compared to female 25(20.3%). The age group, >45(nearly 33%) with median age 42± 21.5, were prone to the infection. During the study period, 4.6% (515/11048) sputum samples were reported as smear-positive in TUTH; consequently, with Xpert MTB/RIF assay, additional case 16.5% (n=85/515) missed on the smear microscopy, were detected—surging overall confirmed cases. Among the most occurring clinical presentations, cough and chest pain were more evident in PTB with relative-risk at 95% confident-levels i.e. 3.03(1.01-9.11) and 3.47(2.29-5.27) respectively. A higher number of new suspects (n=63) were found positive compared to previously treated suspects. The upper lobe infiltrates (36.4%) and pleural effusion (40.4%) were peculiar radiological impression noted in PTB patient. 94 MDR suspected cases were enrolled; of total suspected cases, 29 samples were found rifampicin sensitive, 1 indeterminant while 2 of them were rifampicin-resistant. However, a single rifampicin-resistant; case was detected in patient which was not MDR suspected. Conclusion Additional cases of PTB which are neglected as smear-negative on microscopy and other conventional tests can be detected with gene Xpert test. Hence, recommended to every suspect as a presumptive test could be a wise investment in diagnosis to restrict the global burden to some extent. Keywords: Xpert MTB/Rif assay, Mycobacterium tuberculosis, Line Probe Assay, MDR-TB

scholarly journals Light Emitting Diode (LED) Fluorescent Microscopy: a Milestone in the Detection of Paucibacillary Mycobacterium in Case of Pulmonary Tuberculosis

1970 ◽  
Vol 40 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Z Khatun ◽  
MS Hossain ◽  
CK Roy ◽  
T Sultana ◽  
MQ Rahman ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Hiv Risk ◽  
Gold Standard ◽  
Risk Evaluation ◽  
Financial Constraints ◽  
Light Emitting Diode ◽  
Fluorescent Microscopy ◽  
Light Emitting ◽  
Fluorescent Microscope ◽  
Lowenstein Jensen

In Bangladesh with a large number of pulmonary tuberculosis cases and financial constraints with high HIV risk, evaluation of scanty i.e paucibacillary cases has great importance. To study the efficacy of Light Emitting Diode fluorescent microscopy in the diagnosis of pulmonary tuberculosis specially paucibacillary cases in comparison to conventional fluorescent microscopy, Ziehl-Neelsen staining and culture of sputum samples from patients suspected of pulmonary tuberculosis. 150 sputum samples collected from the patients suspected of pulmonary tuberculosis were processed by the Petroff's method, and subjected to Ziehl-Neelsen staining (ZN), which were examined by both LED and conventional fluorescent microscope (CFM) and culture on Lowenstein- Jensen media (gold standard) for detection of Mycobacterium tuberculosis. In this study, out of 150 patients 14.67%, 8.67%, 4% cases were detected as paucibacillary (Scanty) cases by LED, CFM, ZN respectively. LED fluorescent microscopy is more effective in the detection of paucibacillary cases of pulmonary tuberculosis than other methods of microscopic examination. DOI: http://dx.doi.org/10.3329/bmj.v40i1.9958 BMJ 2011; 40(1): 22-26

scholarly journals Light emitting diode fluorescence microscopy versus Ziehl–Neelsen smear microscopy for the diagnosis of pulmonary tuberculosis

2016 ◽  
Vol 20 (5) ◽  
pp. 522-523
Author(s):  
Shambhu Kumar Upadhyaya ◽  
Narayan Dutt Pant ◽  
Raju Bhandari ◽  
Asia Poudel ◽  
Bhawana Shrestha
Keyword(s):  
Fluorescence Microscopy ◽  
Pulmonary Tuberculosis ◽  
Light Emitting Diode ◽  
Smear Microscopy ◽  
Light Emitting

Detection of Mycobacterium tuberculosis in smear negative sputum by PCR

2012 ◽  
Vol 6 (2) ◽  
pp. 2-6 ◽  
Author(s):  
Mohammad Jobayer ◽  
SM Shamsuzzaman ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Accurate Method ◽  
Major Health Problem ◽  
Major Health ◽  
Tuberculosis Patients ◽  
Diagnostic Potential ◽  
Total Death ◽  
Smear Negative ◽  
Negative Sputum

Pulmonary tuberculosis is a major health problem in Bangladesh that is responsible for about 7% of total death in a year. This study was conducted to isolate and identify Mycobacterium tuberculosis from sputum and to evaluate the efficacy of PCR as a modern diagnostic tool, for diagnosis of pulmonary tuberculosis, especially in the smear negative cases. One hundred and fifty suspected pulmonary TB patients (male/ female: 97/53) were included in this study. Single morning sputum was collected from each patient and diagnostic potential of PCR was compared with staining and culture. Twenty five (16.7%) sputum were positive by ZN stained smear. Among 125 smear negative samples, 13 (10.4%) yielded growth in culture in LJ media and 21 (16.8%) samples were positive by PCR. The sensitivity and specificity of PCR in smear negative cases was 100% and 92.9% respectively. Mean detection time in PCR was 24 hours. PCR detected M. tuberculosis in 21 smear negative and 9 culture negative samples. For diagnosis of tuberculosis in smear negative cases, PCR directly from sputum was a very sensitive and accurate method. In conclusion, PCR may be done, especially in clinically suspected pulmonary tuberculosis patients who remain negative by conventional methods.DOI: http://dx.doi.org/10.3329/bjmm.v6i2.19368 Bangladesh J Med Microbiol 2012; 06(02): 2-6

Role of fibreoptic bronchoscopy in sputum smear negative pulmonary tuberculosis

1994 ◽  
Vol 75 ◽  
pp. 29
Author(s):  
A. McDaniel ◽  
N. Schoenfeld ◽  
W. Frank ◽  
A. Grassot ◽  
H. Mauch ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Fibreoptic Bronchoscopy ◽  
Sputum Smear ◽  
Smear Negative

Anode modification of polymer light-emitting diode using graphene oxide interfacial layer: The role of ultraviolet-ozone treatment

2013 ◽  
Vol 103 (7) ◽  
pp. 073305 ◽  
Author(s):  
Xiao-Chen Jiang ◽  
Yan-Qing Li ◽  
Yan-Hong Deng ◽  
Qi-Qi Zhuo ◽  
Shuit-Tong Lee ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Interfacial Layer ◽  
Light Emitting Diode ◽  
Ozone Treatment ◽  
Light Emitting ◽  
Anode Modification ◽  
Polymer Light Emitting Diode
Sign in / Sign up

Export Citation Format

Share Document