scholarly journals Usefulness of Light Emitting Diode (LED) fluorescent microscopy as a tool for rapid and effective method for the diagnosis of pulmonary tuberculosis

1970 ◽  
Vol 37 (1) ◽  
pp. 7-10 ◽  
Author(s):  
Z Khatun ◽  
M Kamal ◽  
CK Roy ◽  
T Sultana ◽  
MQ Rahman ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Pulmonary Tuberculosis ◽  
Light Emitting Diode ◽  
Fluorescent Microscopy ◽  
Infectious Agent ◽  
Sputum Smear ◽  
Light Emitting ◽  
Brightfield Microscopy ◽  
The Difference ◽  
A Prospective Study

Background: Tuberculosis remains world's leading cause of death from a single infectious agent. Fluorescence microscopy offers well-described benefits, comparing with brightfield microscopy, for the evaluation sputum smear samples for tuberculosis. We evaluated the diagnostic performance of fluorescence microscopy, using novel Light Emitting Diode (LED) technology as an alternative to the conventional fluorescence microscopy by Auramine stain as well as brightfield microscopy by Ziehl- Neelsen (ZN) stain.Objectives: The objective of the study was to see the usefulness of LED fluorescent microscopy in the diagnosis of pulmonary tuberculosis.Methods: This is a prospective study consisted of 150 sputum samples from the patients of NIDCH, Mohakhali. All samples were stained by auramine and ZN stain at BSMMU and culture was done in Lowenstein-Jensen (L-J) media as gold standard at NTRL, Mohakhali.Results: In this study total 66(44%) out of 150 sputum specimens were positive for Mycobacterium Tuberculosis by culture. Sensitivity and specificity documented for the different modalities were 95.38% and 94.11%, respectively, for the LED assessment; 68.18% and 90.47%, respectively, for the CFM assessment; and 56.06% and 97.61%, respectively, for brightfield microscopy by ZN stain. The difference in their case detection rate was statistically significant (χ2=119.38, p<0.001).Conclusion: Fluorescence Microscopy (FM) is more sensitive than ZN for diagnosis of pulmonary tuberculosis. However, since FM is more sensitive and rapid, using this method (LED) in clinical laboratories with large specimen numbers is recommended. DOI: http://dx.doi.org/10.3329/bmrcb.v37i1.7792 Bangladesh Med Res Counc Bull 2011; 37: 7 -10

scholarly journals Role of Light Emitting Diode (LED) Fluorescence Microscopy in the Diagnosis of Smear Negative Pulmonary Tuberculosis.

1970 ◽  
Vol 26 (1) ◽  
pp. 3-6
Author(s):  
Zohra Khatun ◽  
Chandan Kumar Roy ◽  
Tuhin Sultana ◽  
Md Quddusur Rahman ◽  
AN Nashimuddin Ahmed
Keyword(s):  
Fluorescence Microscopy ◽  
Pulmonary Tuberculosis ◽  
Light Emitting Diode ◽  
Light Emitting ◽  
Lowenstein Jensen ◽  
Smear Negative ◽  
Brightfield Microscopy ◽  
Negative Sputum ◽  
A Prospective Study

Light emitting diode (LED) fluorescence microscopy offers well described benefits compared with brightfield microscopy by Ziehl-Neelsen stained sputum, even which are smear negative. We evaluated the diagnostic performance of fluorescence microscopy, using novel light-emitting diode (LED) technology as an alternative to the brightfield microscopy. The objective of this study was the role of LED fluorescence microscopy in diagnosis of smear negative pulmonary tUberculosis. This is a prospective study consisted of 50 smear negative patients, who were clinically suspected cases of pulmonary tuberculosis. All samples were stained by both ZN stain and Auramine stain and as a gold standard all were cultured on Lowenstein-Jensen Media. On evaluation of all sputum samples were found negative by ZN method but by auramine stain 16%, 20%, 20% cases were found positive by conventional fluorescence microscopy (CFM), LED and culture respectively. LED fluorescence microscopy is more useful test to distinguish the smear negative cases. It also provide an effective guideline to make decisions regarding judicious use of antitubercular drug therapy. Keywords: Smear negative Sputum; LED; CFM; Culture; Pulmonary Tuberculosis DOI: http://dx.doi.org/10.3329/bjpath.v26i1.9126 BJPATH 2011; 26(1): 3-6

scholarly journals Fluorescence microscopy for the diagnosis of smear-negative pulmonary tuberculosis in Ethiopia

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Gemeda Abebe ◽  
Dossegnaw Aragaw ◽  
Mulualem Tadesse
Keyword(s):  
Confidence Interval ◽  
Fluorescence Microscopy ◽  
Pulmonary Tuberculosis ◽  
Diagnostic Performance ◽  
Light Emitting Diode ◽  
Fluorescent Microscopy ◽  
Culture Method ◽  
Light Emitting ◽  
Smear Negative ◽  
Negative Sputum

Background: Despite its low sensitivity, microscopy remains the main method for the diagnosis of pulmonary tuberculosis in most laboratories in Ethiopia. Few studies have evaluated the performance of light-emitting diode fluorescent microscopy (LED-FM) in bleach-concentrated smear-negative sputum specimens.Objective: This study aimed to evaluate the diagnostic performance of LED-FM for smear-negative pulmonary tuberculosis in Ethiopia.Methods: A total of 194 adult patients with a cough lasting for more than two weeks, and who had three direct smear-negative sputum tests for Mycobacterium tuberculosis by Ziehl-Neelsen light microscopy, were included. All direct Ziehl-Neelsen-stained smear-negative sputum samples were cultured and were also visualised by LED-FM. Smears for LED-FM were performed from bleach-concentrated sputum sediment. The diagnostic performance of the LED-FM was compared to the culture method (the reference standard).Results: Of the 194 smear-negative sputum specimens analysed, 28 (14.4%) were culture-positive and 21 (10.8%) were LED-FM-positive for M. tuberculosis. However, only 11 of the 21 (52.4%) LED-FM-positive patients had a confirmed tuberculosis diagnosis by culture. Light-emitting diode fluorescence microscopy (FM) had a sensitivity of 39.3% (95% confidence interval: 21.2–57.4) and specificity of 93.9% (95% confidence interval: 90.4–97.6). Ten LED-FM-positive specimens were culture-negative, and all of these specimens had scanty grading (1–19 bacilli per 40 fields on LED-FM).Conclusion: This study showed that implementation of LED-FM on bleach pre-treated and concentrated sputum can significantly improve the diagnosis of smear-negative pulmonary tuberculosis. However, all scanty grade, positive smears by LED-FM need to be confirmed by reference culture method.

scholarly journals Light Emitting Diode (LED) Fluorescent Microscopy: a Milestone in the Detection of Paucibacillary Mycobacterium in Case of Pulmonary Tuberculosis

1970 ◽  
Vol 40 (1) ◽  
pp. 22-26 ◽  
Author(s):  
Z Khatun ◽  
MS Hossain ◽  
CK Roy ◽  
T Sultana ◽  
MQ Rahman ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Hiv Risk ◽  
Gold Standard ◽  
Risk Evaluation ◽  
Financial Constraints ◽  
Light Emitting Diode ◽  
Fluorescent Microscopy ◽  
Light Emitting ◽  
Fluorescent Microscope ◽  
Lowenstein Jensen

In Bangladesh with a large number of pulmonary tuberculosis cases and financial constraints with high HIV risk, evaluation of scanty i.e paucibacillary cases has great importance. To study the efficacy of Light Emitting Diode fluorescent microscopy in the diagnosis of pulmonary tuberculosis specially paucibacillary cases in comparison to conventional fluorescent microscopy, Ziehl-Neelsen staining and culture of sputum samples from patients suspected of pulmonary tuberculosis. 150 sputum samples collected from the patients suspected of pulmonary tuberculosis were processed by the Petroff's method, and subjected to Ziehl-Neelsen staining (ZN), which were examined by both LED and conventional fluorescent microscope (CFM) and culture on Lowenstein- Jensen media (gold standard) for detection of Mycobacterium tuberculosis. In this study, out of 150 patients 14.67%, 8.67%, 4% cases were detected as paucibacillary (Scanty) cases by LED, CFM, ZN respectively. LED fluorescent microscopy is more effective in the detection of paucibacillary cases of pulmonary tuberculosis than other methods of microscopic examination. DOI: http://dx.doi.org/10.3329/bmj.v40i1.9958 BMJ 2011; 40(1): 22-26

scholarly journals Diagnosis of pulmonary tuberculosis with cartridge based nucleic acid amplification test and light emitting diode fluorescent microscopy: a comparative study

2019 ◽  
Vol 6 (5) ◽  
pp. 1580 ◽  
Author(s):  
Pratik Kumar ◽  
Puneet Bhardwaj
Keyword(s):  
Nucleic Acid ◽  
Pulmonary Tuberculosis ◽  
Negative Predictive Value ◽  
Predictive Value ◽  
Light Emitting Diode ◽  
Fluorescent Microscopy ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Smear Microscopy ◽  
Light Emitting

Background: Due to low sensitivity and inability to detect drug resistance, smear microscopy limits its impact on TB control. Culture methods and drug susceptibility testing is complex, time consuming, and takes around 6-8 weeks. A new diagnostic test, cartridge based nucleic acid amplification test (CBNAAT) was developed based on real-time polymerase chain reaction (RT PCR). Objective of this study to compare the results of CBNAAT for diagnosis of pulmonary tuberculosis with LED fluorescent microscopy and sputum culture.Methods: A cross-sectional study was conducted in the department of Chest and TB, CIMS, Bilaspur. Each Sputum sample of presumptive TB patients were tested with CBNAAT, sputum smear  microscopy by light emitting diode (LED) fluorescent microscopy (FM) and solid and liquid culture for diagnosis of Tuberculosis. Results of CBNAAT, Fluorescent Microscopy and Culture for detection of Mycobacterium Tuberculosis were compared.Results: The sensitivity and specificity for CBNAAT were 97% and 100% respectively; while that for Fluorescent microscopy were 70% and 100% respectively. The positive and negative predictive value for CBNAAT was 100% and 96% respectively. The positive and negative predictive value for Fluorescent microscopy was 100% and 73% respectively.Conclusion: CBNAAT is having high sensitivity and specificity for diagnosis of pulmonary tuberculosis. It should be routinely used under national health programme to detect a tuberculosis case efficiently.

scholarly journals A comparative study of fluorescent microscopy with Ziehl-Neelsen staining and culture for the diagnosis of pulmonary tuberculosis

1970 ◽  
Vol 7 (3) ◽  
pp. 226-230 ◽  
Author(s):  
S Laifangbam ◽  
HL Singh ◽  
NB Singh ◽  
KM Devi ◽  
NT Singh
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Fluorescence Microscopy ◽  
Pulmonary Tuberculosis ◽  
Fluorescent Microscopy ◽  
False Negative ◽  
Diagnostic Methods ◽  
Case Detection ◽  
Detection Rates ◽  
Auramine O ◽  
The Difference

Background: For developing countries with a large number of cases and financial constraints, evaluation of rapid and inexpensive diagnostic methods has great importance. The bacilli in the sputum can be detected microscopically by ZN stain and fluorochrome stain. Objectives : To study the efficacy of fluorescence microscopy in the diagnosis of pulmonary tuberculosis in comparison to Ziehl-Neelsen staining and culture of sputum samples from patients suspected of pulmonary tuberculosis. Materials and methods : 306 sputum samples collected from 102 patients suspected of pulmonary tuberculosis were processed by the Petroff's method, and subjected to Ziehl-Neelsen staining (ZN), fluorescent Auramine-O staining (AO) and culture on modified Lowenstein-Jensen media (gold standard) for detection of Mycobacterium tuberculosis. Positive smears were graded according to Forbes BA et al, and culture isolates were biochemically tested for confirmation of species. Results : Out of 102 patients, 44.1%, 71.6% and 70% were found positive by ZN, AO and culture respectively. AO was found to be superior to ZN on several aspects. The difference in their case detection rates was statistically significant (χ2 = 24.93, p < 0.001). AO was also able to detect more pauci-bacillary cases than ZN. There was more agreement between culture and fluorescence microscopy (95.1%) than with ZN microscopy (69.6%). The percentage of false negative by AO staining was only 2.78% which was in sharp contrast to that of ZN (40.27%). Conclusion: The better case detection rates of AO over ZN were comparable to those found by several studies. Since screening was done under lower power of magnification (400x), fluorescence microscopy has been found to be less time consuming as compared to ZN method (1000x) in the diagnosis of tuberculosis. The tubercle bacilli stood out as bright objects against a dark background in fluorescence microscopy which makes them easily identifiable hence causing less eye-strain. The efficacy of fluorescence microscopy proved to be much higher than conventional light microscopy and comparable to that of culture. Key words: Ziehl-Neelsen staining; Mycobacterium tuberculosis; Auramine-O DOI: 10.3126/kumj.v7i3.2728 Kathmandu University Medical Journal (2009) Vol.7, No.3 Issue 27, 226-230

scholarly journals Comparison of GeneXpert against Light-Emitting Diode Fluorescent Microscopy for the Diagnosis of Pulmonary Tuberculosis in Addis Ababa, Ethiopia

2018 ◽  
Vol 10 (1) ◽  
Author(s):  
Boja Dufera Taddese ◽  
Daniel Melese Desalegn ◽  
Abay Sisay Misganaw ◽  
Kumera Terfa Kitila ◽  
Hanna Mekonnen Balcha ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Light Emitting Diode ◽  
Addis Ababa ◽  
Fluorescent Microscopy ◽  
Light Emitting
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