scholarly journals Optimization of Flooded Soil Recovery via Plant- Arbuscular Mycorrhizal Fungi Symbiotic Interaction

2017 ◽  
Vol 19 ◽  
pp. 67
Author(s):  
Nor Hazwani Aziz ◽  
Norazwina Zainol ◽  
Nanthinie Thangaperumal ◽  
Nor Hanisah Zahari

<p>Flooded soil recovery was optimized using experimental design methodology by manipulating the symbiotic relationship between soil fungi, Arbuscular Mycorrhizal Fungi (AMF) and the host plant (Allium cepa L.) planted in a soil containing AMF (SA). This was achieved by measuring the amount of nutrient (nitrogen, phosphorus and potassium) uptake by AMF using HACH spectrophotometer after 14 days of planting in several condition suggested by Design-Expert® software (Ver 7.1.6). In order to determine the optimum condition for the AMF to recover the flooded soil, the experiments were designed according to a central composite design in two variables following the Response Surface Methodology (RSM). A quadratic polynomial model was generated to predict soil recovery. R2 for nitrogen, phosphorus and potassium was found at 0.89, 0.96 and 0.94 respectively of the range for the factors studied namely 24-32 ml water content and 4.0-6.0 cm depth of soil. Among two parameters, depth of soil showed significant effect on the recovery of flooded soil for phosphorus and potassium while for nitrogen both parameters showed insignificant effect. Model validation experiments showed good correspondence between experimental and predicted values at error for N, P, and K at 7.0%, 1.86% and 2.65% respectively. The optimal condition for soil recovery was at 28 ml soil water content and 5 cm soil depth. At this condition, the nutrient uptake by AMF was predicted to be at their maximum rate where the concentration of nutrients increased approximately by 2 to 3 times from the initial nutrient concentration.</p><p>Chemical Engineering Research Bulletin 19(2017) 67-74</p>

2014 ◽  
Vol 45 (2) ◽  
pp. 585-594 ◽  
Author(s):  
Alejandra Becerra ◽  
Norberto Bartoloni ◽  
Noelia Cofré ◽  
Florencia Soteras ◽  
Marta Cabello

2020 ◽  
Vol 13 ◽  
pp. 117862212096919
Author(s):  
James Peter Muchoka ◽  
Daniel Njiru Mugendi ◽  
Paul Nthakanio Njiruh ◽  
Charles Onyari ◽  
Paul Kamau Mbugua ◽  
...  

The use of arbuscular mycorrhizal fungi (AMF) to enhance soil phosphate uptake is a biological technique considered to cure phosphate deficiency in soils. This study investigated association of Aspilia pruliseta Schweif shrub with AMF in Kenya. The study aims at profiling a tropical shrub with multiple ecological benefits that could reduce addition of chemical phosphatic fertilizer into the soil and reverse negative consequences of eutrophication. Sampling was purposive to have areas with or without Aspilia pruliseta vegetation growing. A small amount (10 g) of the soil from 27 composite samples was used for spore count determination and a similar amount for next generation sequencing. Spore counts varied significantly among soil textural types, sample locations, and soil depth. Sandy loam had the highest spore counts with a mean average of 404 spores. The spore count decreased significantly ( P < .05) with the depth of soil from a mean of 514 spores to 185 along the rhizosphere. The intensity of spore morphotypes was significantly higher at P < .05 for soils whose vegetation was covered with Aspilia pruliseta than those without. Aspilia pruliseta vegetation used together with sandy loam soil could culture commercial mycorrhiza fungi production for use in agrisystems.


2020 ◽  
Vol 17 (4) ◽  
pp. 150
Author(s):  
O. TRISILAWATI

<p>ABSTRACT</p><p>The effects of several arbuscular mycorrhizal fungi (AMF) on thegrowth, nutrient uptake (nitrogen, phosphorus, and potassium), and acidphosphate activity of two promising numbers of Anacardium occidentaleseedling were evaluated. The experiment was conducted in the greenhouse of Indonesian Spices and Medicinal Crops Research Institute(BALITTRO) in 2002 for six months on a randomized design with twofactors and four replicates. First factor was isolate (six isolates of AMFand one control) consisting of : 1) control; 2) Glomus aggregatum; 3)Glomus etunicatum; 4) Mycofer; 5) Glomus sp.; 6) a mixture of Glomussp1, Glomus sp2, Glomus sp3, Glomus sp4, Glomus etunicatum,Gigaspora margarita, Gigaspora sp., and Enthropospora sp., and 7)Gigaspora sp. The second factor was two cashew promising numbers :Asembagus and Wonogiri. The results showed that AMF inoculationsignificantly affected the growth of cashew. Mycofer and mixed AMFwere more effective to Wonogiri promising number, while for Asembaguspromising number inoculation of mycofer was more effective. Inoculationwith mycofer to Asembagus promising number increased the uptake of Pand K nutrients by 65 and 53% while inoculation with mycofer and mixedAMF to Wonogiri promising number increased the uptake of N, P and Knutrients by 55, 38, and 17%, and by 18, 31, and 17%. Moreover, theAMF inoculation resulted in higher phosphatase activity. In mycorrhizalAsembagus promising number infected by mixed AMF, the increment ofphosphatase activity was 136.5%, whether in Wonogiri promising numberinfected by mycofer, the increment of phosphatase activity was 80% thancontrol.</p><p>Key words: Anacardium occidentale, promising number, growth,phosphatase activity</p><p>ABSTRAK</p><p>Pengaruh Pupuk Hayati Fungi Mikoriza Arbuskula(FMA) terhadap Pertumbuhan Benih Jambu Mete</p><p>Penelitian ini bertujuan untuk mengetahui pengaruh beberapa jenisfungi mikoriza arbuskula (FMA) terhadap pertumbuhan, serapan hara danaktivitas enzim fosfatase dari dua nomor harapan benih jambu mete(Anacardium occidentale). Penelitian dilakukan di rumah kaca Balittropada tahun 2002 selama 6 bulan, menggunakan rancangan acak yangterdiri dari dua faktor dan diulang empat kali. Faktor pertama adalahisolate (6 jenis isolat FMA dan satu kontrol) yaitu: 1). kontrol; 2). Glomusaggregatum; 3) Glomus etunicatum; 4). Mycofer; 5). Glomus sp.; 6).campuran dari Glomus sp1, Glomus sp2, Glomus sp3, Glomus sp4, Glomusetunicatum, Gigaspora margarita, Gigaspora sp., Enthropospora sp., dan7). Gigaspora sp. Faktor kedua adalah nomor harapan jambu mete, yaituAsembagus dan Wonogiri. Hasil penelitian mendapatkan bahwa inokulasiFMA berpengaruh nyata terhadap pertumbuhan jambu mete. Mycofer dancampuran FMA lebih efektif berpengaruh terhadap nomor harapanWonogiri, sedangkan mycofer lebih efektif berpengaruh terhadap nomorharapan Asembagus. Serapan hara P dan K pada nomor harapanAsembagus yang diinokulasi mycofer meningkat sebesar 65 dan 53%,sedangkan nomor harapan Wonogiri yang diinokulasi mycofer dancampuran FMA, serapan hara N, P, dan K meningkat masing-masingsebesar 55; 38; dan 17%, dan 18; 31; dan 17%. Selain itu, inokulasi FMAdapat meningkatkan aktivitas fosfatase akar jambu mete. Peningkatanaktivitas fosfatase akar jambu mete nomor harapan Asembagus yangterinfeksi oleh campuran FMA sebesar 136,5%, sedangkan pada nomorharapan Wonogiri yang terinfeksi mycofer, peningkatnnya sebesar 80%dibandingkan kontrol.</p><p>Kata kunci: Anacardium occidentale, nomor harapan, pertumbuhan,aktivitas fosfatase</p>


Plants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 80 ◽  
Author(s):  
Abderrahim Boutasknit ◽  
Marouane Baslam ◽  
Mohamed Ait-El-Mokhtar ◽  
Mohamed Anli ◽  
Raja Ben-Laouane ◽  
...  

Irregular precipitation and drought caused an increase in tree mortality rates in multiple forest biomes with alterations in both ecosystem services and carbon balance. Carob (Ceratonia siliqua) growth and production in arid and semi-arid ecosystems are likely affected by climate change-induced droughts. Understanding the physiological responses of drought-induced early-stage tree death and strategies to enhance drought tolerance and optimize growth will help tree improvement programs. Mycorrhizal inoculation has a pronounced impact on plant growth, water absorption, mineral nutrition, and protection from abiotic stresses. However, a better understanding of these complex interconnected cellular processes and arbuscular mycorrhizal fungi (AMF)-mediated mechanisms regulating drought tolerance in plants will enhance its potential application as an efficient approach for bio-amelioration of stresses. The objectives of this work were to elucidate the different effects of autochthone AMF on inorganic solute and water content uptakes, organic adjustments (sugar and proteins content), leaf gas exchange (stomatal conductance and efficiency of photosystems I and II), and oxidative damage of two contrasting ecotypes of carob seedlings: coastal (southern ecotype (SE)) and in-land (northern ecotype (NE)) under control (C), drought (by cessation of irrigation for 15 days (15D)), and recovery (R) conditions. Our findings showed that AMF promoted growth, nutrient content, and physiological and biochemical parameters in plants of both ecotypes during C, 15D, and R conditions. After four days of recovery, stomatal conductance (gs), the maximum photochemical efficiency of PSII (Fv/Fm), water content, and plant uptake of mineral nutrients (P, K, Na, and Ca) were significantly higher in shoots of mycorrhizal (AM) than non-mycorrhizal (NM) control plants. Consequently, AMF reduced to a greater degree the accumulation of hydrogen peroxide (H2O2) and oxidative damage to lipid (malondialdehyde (MDA)) content in AM than NM plants in NE and SE, after recovery. Altogether, our findings suggest that AMF can play a role in drought resistance of carob trees at an early stage by increasing the inorganic solutes (P, K, Na, and Ca), water content uptake, organic solutes (soluble sugars and protein content), stomatal conductance, and defense response against oxidative damage during re-watering after drought stress.


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