scholarly journals Arbuscular Mycorrhizal Fungi Colonization in the Rhizosphere of Aspilia pruliseta Schweif. ext Schweif in the Semiarid Eastern Kenya

2020 ◽  
Vol 13 ◽  
pp. 117862212096919
Author(s):  
James Peter Muchoka ◽  
Daniel Njiru Mugendi ◽  
Paul Nthakanio Njiruh ◽  
Charles Onyari ◽  
Paul Kamau Mbugua ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Sandy Loam ◽  
Soil Depth ◽  
Arbuscular Mycorrhizal ◽  
Phosphate Deficiency ◽  
Similar Amount ◽  
Negative Consequences ◽  
Spore Count ◽  
Biological Technique

The use of arbuscular mycorrhizal fungi (AMF) to enhance soil phosphate uptake is a biological technique considered to cure phosphate deficiency in soils. This study investigated association of Aspilia pruliseta Schweif shrub with AMF in Kenya. The study aims at profiling a tropical shrub with multiple ecological benefits that could reduce addition of chemical phosphatic fertilizer into the soil and reverse negative consequences of eutrophication. Sampling was purposive to have areas with or without Aspilia pruliseta vegetation growing. A small amount (10 g) of the soil from 27 composite samples was used for spore count determination and a similar amount for next generation sequencing. Spore counts varied significantly among soil textural types, sample locations, and soil depth. Sandy loam had the highest spore counts with a mean average of 404 spores. The spore count decreased significantly ( P < .05) with the depth of soil from a mean of 514 spores to 185 along the rhizosphere. The intensity of spore morphotypes was significantly higher at P < .05 for soils whose vegetation was covered with Aspilia pruliseta than those without. Aspilia pruliseta vegetation used together with sandy loam soil could culture commercial mycorrhiza fungi production for use in agrisystems.

scholarly journals Arbuscular mycorrhizal fungi in saline soils: vertical distribution at different soil depth

2014 ◽  
Vol 45 (2) ◽  
pp. 585-594 ◽  
Author(s):  
Alejandra Becerra ◽  
Norberto Bartoloni ◽  
Noelia Cofré ◽  
Florencia Soteras ◽  
Marta Cabello
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Vertical Distribution ◽  
Mycorrhizal Fungi ◽  
Soil Depth ◽  
Arbuscular Mycorrhizal ◽  
Saline Soils

scholarly journals Isolation and identification of indigenous Arbuscular Mycorrhizal Fungi (AMF) of forest clove rhizosphere from Maluku, Indonesia

2021 ◽  
Vol 22 (8) ◽  
Author(s):  
Asri Subkhan Mahulette ◽  
Anggra Alfian ◽  
ABDUL KARIM KILKODA ◽  
IMELDA JEANETTE LAWALATA ◽  
DESSY ARIYANI MARASABESSY ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Soil Samples ◽  
Spore Density ◽  
Wild Type ◽  
Isolation And Identification ◽  
Spore Count ◽  
Spore Number ◽  
Total Spore Count

Abstract. Mahulette AS, Alfian A, Kilkoda KA, Lawalata IJ, Marasabessy DA, Tanasale VL, Makaruku MH. 2021. Isolation and identification of indigenous Arbuscular Mycorrhizal Fungi (AMF) of forest clove rhizosphere from Maluku, Indonesia. Biodiversitas 22: 3613-3619. Forest clove is classified as wild-type and endemic to the Maluku (Moluccas) Islands, Indonesia. The different condition of growing areas causes various types of Arbuscular Mycorrhizal Fungi (AMF) associated with forest clove. The study aimed to identify and obtain indigenous AMF inoculums from the forest clove rhizosphere from two distribution areas in Maluku. The results of AMF identification found two types of spores from the genus Glomus in the rhizosphere of forest cloves from Ambon Island with a spore density of 35/50 g of soil. In comparison, three spores were found in Seram Island, two from the genus Scutellospora and one from the Acaulospora. With an overall spore density of 5/50 g of soil. After culture trapping, there was a change in type and an increase in spore density in soil samples from the rhizosphere of the two forest clove distribution areas. Soil samples from Ambon after trapping culture obtained two new types of spores from the genus Acaulospora with a total spore number of 57/50 g soil while in soil samples from Seram found three new types of spores from the genus Glomus with a total spore count of 104/50 g of soil.

Yield and Rhizosphere - microflora of Cowpea in Response to Magnesium Fertilization in Lateritic Soils of Kerala

2021 ◽  
Author(s):  
V.P. Soniya ◽  
P.S. Bhindhu
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Root Colonization ◽  
Arbuscular Mycorrhizal ◽  
Nitrogen Fixing ◽  
Nitrogen Fixing Bacteria ◽  
Free Living ◽  
Spore Count ◽  
Lateritic Soils ◽  
Magnesium Uptake

Background: Magnesium deficiency has become a major nutritional disorder in lateritic soils of Kerala. Appropriate magnesium fertilization is the best strategy to combat deficiency issues. Apart from correcting nutritional deficiency, magnesium fertilization has an influence on the growth of beneficial microbes such as nitrogen fixing bacterias and arbuscular mycorrhizal fungi. The experiment aimed to investigate the effect of magnesium fertilization on crop yield and population rhizosphere micoflora of cowpea in lateritic soils of Kerala.Methods: A pot culture experiment was conducted with a gradient of magnesium additions ranging from 5 mg kg-1 to 80 mg kg-1 of soil along with recommended dose of fertilizers. Population of rhizobium, free living nitrogen fixing bacteria, spore count of arbuscular mycorrhizal fungi and per cent root colonization of arbuscular mycorrhizal fungi were studied during flowering. The available magnesium and magnesium uptake were also worked out during harvest. Yield and yield contributing characteristics of cowpea were measured during harvest stage.Result: Magnesium addition produced significant variations in population of rhizobium and free- living nitrogen fixing bacteria whereas spore count of AMF and per cent root colonization of AMF did not vary according to the added doses of magnesium. A higher population of rhizobium, free living nitrogen fixers, root nodules, magnesium uptake, plant height and yield were obtained in the treatment where magnesium was applied @ 10 mg kg-1 soil.

scholarly journals Effects of arbuscular mycorrhizal fungi and Rhizobium on ion content and root characteristics of green bean and maize under intercropping

2017 ◽  
Vol 109 (1) ◽  
pp. 79 ◽  
Author(s):  
Zahra Marzban ◽  
Elham Faryabi ◽  
Shahram Torabian
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Root Growth ◽  
Mycorrhizal Fungi ◽  
Sandy Loam ◽  
Total Concentration ◽  
Arbuscular Mycorrhizal ◽  
Green Bean ◽  
Root Characteristics ◽  
P Concentration ◽  
Sole Cropping

<p>In order to evaluate arbuscular mycorrhizal fungi and rhizobium bacteria effects on leaf nitrogen (N) and phosphorus (P) concentration and root characteristics of green bean and maize under intercropping, experiment was carried out in the research field of College of Agriculture, Payame Noor University of Azna, Lorestan, Iran. In experiment, sandy loam soil with pH 7.3 and EC 0.49 dS m<sup>-1</sup> was used.The treatments comprised three cropping systems (sole cropping of green bean and maize, and intercropping), and four inoculations (control, arbuscular mycorrhizal fungi, rhizobium and mix of arbuscular mycorrhizal fungi and rhizobium). The results showed that inoculation with rhizobium improved length, diameter, volume and area of green bean root.The highest of green bean N, P concentration and root dry mass were observed in sole culture of green bean inoculated with arbuscular mycorrhizal fungi. Moreover, root length, diameter, volume and area of maize increased by arbuscular mycorrhizal fungi, and total concentration of N and P enhanced with use of rhizobium in sole cropping. Although the usage of <em>Rhizobium </em>and AMF can be affected on increasing the root growth and nutrient uptake of crops, application of bacterium and fungi combination at the same time would not be suitable. Overall, intercropping of maize with green bean caused to increase of leaf N and P concentrations and root growth of maize.</p>

Concentrations of K, Ca and Mg in maize colonized by arbuscular mycorrhizal fungi under field conditions

2002 ◽  
Vol 82 (3) ◽  
pp. 272-278 ◽  
Author(s):  
A. Liu ◽  
C. Hamel ◽  
A. Elmi ◽  
C. Costa ◽  
B. Ma ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Sandy Loam ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Dry Weight ◽  
Soil Fumigation ◽  
Soil P ◽  
Shoot Dry Weight ◽  
Loamy Sand Soil

Little attention has been paid to the effect of arbuscular mycorrhizal (AM) fungi on the uptake of nutrients that move mainly by mass flow. The objective of this study was to assess the possible contribution of indigenous AM fungi to the K, Ca and Mg nutrition of maize (Zea mays L.) as influenced by soil P levels and its impact on plant dry mass. The field experiment had a split plot design with four replicates. Treatments included soil fumigation status (fumigation and non-fumigation) and three levels of P fertilization (0, 60 and 120 kg P2O5 ha-1) in a loamy sand soil in 1997 and a fine sandy loam soil in 1998. Soil fumigati on with Basamid® was used to suppress indigenous AM fungi. Plants were sampled at four different growth stages (6-leaf stage, 10-leaf stage, tasseling and silking). Soil fumigation decreased shoot dry weight, but P fertilization increased shoot dry weight at most sampling times. When no P fertilizer was added, fumigation in the loamy sand soil reduced shoot K and Ca concentrations while, in contrast, in the fine sandy loam soil only Mg concentration was reduced by soil fumigation. The concentration of K in maize shoots was positively correlated (P < 0.05) with extraradicular hyphal length in both soils. The correlation between the abundance of extraradicular hyphae and the concentrations of Ca and Mg in maize shoots was significant only for soils where available Ca or Mg was relatively low. Arbuscular mycorrhizal fungi could increase corn biomass production and K, Ca and Mg uptake in soil low in these elements and low in P. These results indicate that the contribution of mycorrhizae to maize K, Ca and Mg nutrition can be significant in a field situation and that the extent of this contribution depends on the availability of these nutrients and of P in soils. Key words: Arbuscular mycorrhizal fungi, soil fumigation, extraradicular hyphae, uptake of K, Ca, and Mg, soil P levels, maize

scholarly journals Optimization of Flooded Soil Recovery via Plant- Arbuscular Mycorrhizal Fungi Symbiotic Interaction

2017 ◽  
Vol 19 ◽  
pp. 67
Author(s):  
Nor Hazwani Aziz ◽  
Norazwina Zainol ◽  
Nanthinie Thangaperumal ◽  
Nor Hanisah Zahari
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Water Content ◽  
Mycorrhizal Fungi ◽  
Chemical Engineering ◽  
Soil Depth ◽  
Arbuscular Mycorrhizal ◽  
Flooded Soil ◽  
Phosphorus And Potassium ◽  
Soil Recovery ◽  
Nitrogen Phosphorus

<p>Flooded soil recovery was optimized using experimental design methodology by manipulating the symbiotic relationship between soil fungi, Arbuscular Mycorrhizal Fungi (AMF) and the host plant (Allium cepa L.) planted in a soil containing AMF (SA). This was achieved by measuring the amount of nutrient (nitrogen, phosphorus and potassium) uptake by AMF using HACH spectrophotometer after 14 days of planting in several condition suggested by Design-Expert® software (Ver 7.1.6). In order to determine the optimum condition for the AMF to recover the flooded soil, the experiments were designed according to a central composite design in two variables following the Response Surface Methodology (RSM). A quadratic polynomial model was generated to predict soil recovery. R2 for nitrogen, phosphorus and potassium was found at 0.89, 0.96 and 0.94 respectively of the range for the factors studied namely 24-32 ml water content and 4.0-6.0 cm depth of soil. Among two parameters, depth of soil showed significant effect on the recovery of flooded soil for phosphorus and potassium while for nitrogen both parameters showed insignificant effect. Model validation experiments showed good correspondence between experimental and predicted values at error for N, P, and K at 7.0%, 1.86% and 2.65% respectively. The optimal condition for soil recovery was at 28 ml soil water content and 5 cm soil depth. At this condition, the nutrient uptake by AMF was predicted to be at their maximum rate where the concentration of nutrients increased approximately by 2 to 3 times from the initial nutrient concentration.</p><p>Chemical Engineering Research Bulletin 19(2017) 67-74</p>

Seasonal variation and distribution at different soil depths of arbuscular mycorrhizal fungi spores in a tropical sclerophyllous shrubland

Botany ◽  
2010 ◽  
Vol 88 (1) ◽  
pp. 54-64 ◽  
Author(s):  
Gisela Cuenca ◽  
Milagros Lovera
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Soil Depth ◽  
Arbuscular Mycorrhizal ◽  
Tropical Soils ◽  
Spore Density ◽  
Soil Cores ◽  
Limited Information ◽  
Different Seasons ◽  
Different Depths

Limited information is available for the diversity of arbuscular mycorrhizal fungi (AMF) in most tropical soils. To assess the biodiversity of AMF in a sclerophyllous shrubland ecosystem, an exhaustive collection of spores from soil collected in the Venezuelan Guayana was undertaken. Spores were collected from the first 15 cm of soil, four times throughout the year, and at four different depths during dry and wet seasons. A total of 50 AMF morphotypes were collected in the field, and 2 more were isolated from pot cultures. There was a reduction in spore density with soil depth, although richness of spores remained the same up to a depth of 45 cm. Spore density and richness based on soil cores were higher in the dry season than in the rainy season. Only 24% of the AMF morphotypes present could be identified to species level, since most of them did not fit published descriptions available. It is important to perform an exhaustive sampling of spores in the different seasons throughout the year and at different soil depths to obtain an accurate view of AMF spore diversity associated with a particular ecosystem.

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