Effects of some indigenous plant seed extracts on the Haematology of a predatory fish Singhi Heteropneustes fossilis (Bloch)

Haemolytic effects of different extracts of five indigenous plant seeds viz. Datura innoxia (Mill) (Shada Dhutra), Clerodendrum viscosum (Vent.) (Vat), Amoora rohituka (Roxb.) Wt. ct. Arnott (Pitraj), Acacia auriculiformis A. Cunn. Ex. Benth. (Akashmoni) and Pongamia pinnata (L.) Pierre (Karinja) were studied upon a predatory fish, Singhi, Heteropneustes fossilis (Bloch) under laboratory conditions. Toxicants inducing effects on the blood parameters included decreased RBC and WBC counts, low haemoglobin percentage and decreased PCV (packed cell volume), MCV (mean corpuscular volume) and MCH (mean corpuscular haemoglobin). Haemolysis action included shrunken or swollen RBCs, granulated nuclei of RBCs and pyknotic nuclei of WBCs, clumping of RBCs and syncitium of RBC matrix. Damage of blood cells with absolute ethyl alcohol extract was most pronounced with all of the seed extracts. The order of toxicity of the five seed extracts on the blood parameters of Singhi was: D. innoxia < A. auriculaerformis < P. pinnata < C. viscosum < A. rohituka seed extracts. On the basis of haemolysis the toxicity was: A. rohituka > C. viscosum > P. pinnata > D. innoxia > A. auriculiformis seed extracts. DOI: http://dx.doi.org/10.3329/cujbs.v6i1-2.17078 The Chittagong Univ. J. B. Sci.,Vol. 6(1&2):11-25, 2011

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Effects of some indigenous plant seed extracts on the haematology of a predatory fish singhi Heteropneustes fossilis (Bloch)

Chittagong University Journal of Biological Sciences ◽

10.3329/cujbs.v5i1.13367 ◽

2013 ◽

pp. 27-36

Author(s):

Munira Nasiruddin ◽

Mohammad Ali Azadi ◽

Monika Rahman ◽

Israt Ara Shazia Rahman

Keyword(s):

Blood Parameters ◽

Acacia Auriculiformis ◽

Predatory Fish ◽

Heteropneustes Fossilis ◽

Plant Seed ◽

Alcohol Extract ◽

Plant Seeds ◽

Indigenous Plant ◽

Clerodendrum Viscosum ◽

Seed Extracts

Haemolytic effects of different extracts of five indigenous plant seeds viz. Datura innoxia (Mill) (Shada Dhutra), Clerodendrum viscosum (Vent.) (Vat), Amoora rohituka (Roxb.) Wt. ct. Arnott (Pitraj), Acacia auriculiformis A. Cunn. Ex. Benth. (Akashmoni) and Pongamia pinnata (L.) Pierre (Karinja) were studied upon a predatory fish, Singhi, Heteropneustes fossilis (Bloch) under laboratory conditions. Toxicants inducing effects on the blood parameters included decreased RBC and WBC counts, low haemoglobin percentage and decreased PCV (packed cell volume), MCV (mean corpuscular volume) and MCH (mean corpuscular haemoglobin). Haemolysis action included shrunken or swollen RBCs, granulated nuclei of RBCs and pyknotic nuclei of WBCs, clumping of RBCs and syncitium of RBC matrix. Damage of blood cells with absolute ethyl alcohol extract was most pronounced with all of the seed extracts. The order of toxicity of the five seed extracts on the blood parameters of Singhi was: D. innoxia < A. auriculiformis < P. pinnata < C. viscosum < A. rohituka seed extracts. On the basis of haemolysis the toxicity was: A. rohituka > C. viscosum > P. pinnata > D. innoxia > A. auriculiformis seed extracts. DOI: http://dx.doi.org/10.3329/cujbs.v5i1.13367 The Chittagong Univ. J. B. Sci.,Vol. 5(1 &2):27-36, 2010

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Toxic response of Heteropneustes fossilis (Bloch) to some indigenous plant seed extracts

Chittagong University Journal of Biological Sciences ◽

10.3329/cujbs.v4i1.13382 ◽

2013 ◽

pp. 01-12

Author(s):

Munira Nasiruddin ◽

Monika Rahman ◽

Rozina Akhter

Keyword(s):

Ethyl Alcohol ◽

Water Extract ◽

Distilled Water ◽

Heteropneustes Fossilis ◽

Absolute Ethyl Alcohol ◽

Plant Seed ◽

Alcohol Extract ◽

Toxic Response ◽

Indigenous Plant ◽

Seed Extracts

Toxic responses of distilled water, 50% ethyl and absolute ethyl alcohol extracts of six indigenous plants, Datura innoxia (Mill), Clerodendrum viscosum (Vent), Amoora rohituka (Roxb) Wt. et. Arnott, Acacia auriculaeformis A. Cunn. ex. Benth., Pongamia pinnata (L) Pierre and Acacia catechu Willd were studied on a predatory fish Heteropneustes fossilis (Bloch) in laboratory conditions. Amongst the extracts the toxicity range varied, absolute ethyl alcohol extract of A. rohituka seeds being the most toxic (LC50 = 8.67 ppm) whilst distilled water extract of D. innoxia seeds was the least toxic (LC50 = 1950.03 ppm). On the basis of LC50 and relative potency values the relative toxicity of the six plants was in the order- A. rohituka > C. viscosum > P. pinnata > A. auriculaeformis > A. catechu > D. innoxia seed extracts. These six indigenous plant seed extracts assayed might be helpful as controlling agent of undesirable fishes in fish culture ponds. DOI: http://dx.doi.org/10.3329/cujbs.v4i1.13382 The Chittagong Univ. J. B. Sci.,Vol. 4(1&2):1-12, 2009

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Haemolytic effects of Samanea saman (Jacq.), Tectona grandis (L.) and Dipterocarpus turbinatus (Gaertn) seed extracts on Singhi fish, Heteropneustes fossilis (Bloch)

Journal of the Asiatic Society of Bangladesh Science ◽

10.3329/jasbs.v38i1.15323 ◽

2013 ◽

Vol 38 (1) ◽

pp. 83-91

Author(s):

Munira Nasiruddin ◽

Mohammad Ali Azadi ◽

Shabnam Chowdhury

Keyword(s):

Ethyl Alcohol ◽

Tectona Grandis ◽

Blood Parameters ◽

Cellular Damage ◽

Predatory Fish ◽

Heteropneustes Fossilis ◽

Alcohol Extract ◽

Samanea Saman ◽

Induced Changes ◽

Seed Extracts

The haemolytic effects of distilled water, 50% ethyl alcohol and 100% ethyl alcohol extracts of three plant seeds, Fulkoroi (Samanea saman), shegoon (Tectona grandis) and Gargan (Dipterocarpus turbinatus) were studied on a predatory fish Heteropneustes fossilis under laboratoary conditions. Comparative data of control and treated fishes showed that the toxic components induced changes in the blood parameters. Piscicides inducing toxicity were evident from decreased RBC counts, low haemoglobin percentage and decreased PCV, MCV, MCH values but increased WBC counts. Haemolysis included shrunken or swollen RBC, granulated nuclei of RBC and pyknotic and swollen nuclei of WBC, cellular damage resulting in bare nuclei of RBC and WBC, clumping of RBC and syncitium of RBC matrix. Damage of blood cells with 100% ethyl alcohol extract was pronounced with all the seed extracts. The order of toxicity on the blood parameters statistically were T. grandis > S. saman > D. turbinatus seed extracts. On the basis of haemolysis of blood cell the effects were D. turbinatus > S. saman > T. grandis seed extracts. DOI: http://dx.doi.org/10.3329/jasbs.v38i1.15323 J. Asiat. Soc. Bangladesh, Sci. 38(1): 83-91, June 2012

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Toxicological effect and behavioral response of a predatory Stinging Catfish, Heteropneustes fossilis exposed to three indigenous plant seed extracts

Bangladesh Journal of Zoology ◽

10.3329/bjz.v48i2.52377 ◽

2021 ◽

Vol 48 (2) ◽

pp. 379-391

Author(s):

Sadia Sultana ◽

Munira Nasiruddin ◽

MA Azadi ◽

MR Chowdhury

Keyword(s):

Ethyl Alcohol ◽

Fish Species ◽

Water Extract ◽

Predatory Fish ◽

Distilled Water ◽

Heteropneustes Fossilis ◽

Plant Seed ◽

Toxicological Effect ◽

Indigenous Plant ◽

Seed Extracts

For the removal of undesired fish species from aquaculture ponds, an alternative to synthetic toxins is the use of botanical toxicants which are biodegradable and environmentally safer. Toxicological effects of distilled water, 50% ethyl alcohol, methanol and acetone extracts of three indigenous plant seeds, Jatropha carcus (Linn.), Hydnocarpus wightianus (Blume) and Aleurites moluccana (Linn. Willd) were studied on the predatory fish, Heteropneustes fossilis (Bloch) under normal laboratory conditions. Depending upon the type of plants, extracts and concentrations, percentage mortality varied. Behavioral activities increased with increasing concentrations. During exposure, fish exhibited discolouration, gulping for air, erratic swimming, loss of reflex, slow movement and ultimately became motionless before death . The extent of toxicity of extracts for J. carcus, H. wightianus and A. moluccana on H. fossilis could be ranked in the order: Acetone > methanol > 50% ethyl alcohol > distilled water. On the basis of LC50 values the most toxic was acetone extract of J. carcus seeds and least toxic was distilled water extract of A. moluccana seeds. It is suggested that these plant seed extracts would be helpful in aquaculture to remove unwanted fish species from culture ponds. Bangladesh J. Zool. 48(2): 379-391, 2020

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Piscicidal action and behavioral responses of Cichlid fish Tilapia, Oreochromis niloticus (Linn) exposed to three indigenous plant seed extracts

Egyptian Journal of Aquatic Biology and Fisheries ◽

10.21608/ejabf.2021.149785 ◽

2021 ◽

Vol 25 (1) ◽

pp. 749-760

Author(s):

Sadia Sultana ◽

Munira Nasiruddin ◽

Mohammad Ali Azadi

Keyword(s):

Oreochromis Niloticus ◽

Cichlid Fish ◽

Behavioral Responses ◽

Plant Seed ◽

Indigenous Plant ◽

Seed Extracts

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Histopathological Effects Of Extracts Of Two Indigenous Plants, Pongamia Pinnata (L.) Pierre And Clerodendrum Viscosum (Vent.) On The Cat Fish, Heteropneustes Fossilis (Bloch)

Journal of the Asiatic Society of Bangladesh Science ◽

10.3329/jasbs.v39i1.16039 ◽

2013 ◽

Vol 39 (1) ◽

pp. 105-115 ◽

Cited By ~ 1

Author(s):

Munira Nasiruddin ◽

Mohamad Ali Azadi ◽

Mahmuda Sharmin Nely

Keyword(s):

Pongamia Pinnata ◽

Central Vein ◽

Heteropneustes Fossilis ◽

Indigenous Plants ◽

Alcohol Extract ◽

Plant Parts ◽

Hepatic Cells ◽

Secondary Lamellae ◽

Gill Filaments ◽

Clerodendrum Viscosum

Piscicidal properties of part extracts (seed, leaf and bark) of two indigenous plants, Pongamia pinnata (L.) Pierre and Clerodendrum viscosum (Vent.) were studied on Heteropneustes fossilis (Bloch). A number of histopathological lesions was observed in the tissues of intestine, gill and liver of the fish which were treated with 50% ethyl alcohol extract of the two plant parts toxicants. The principal degenerative changes in the intestine were: disintegrated serosa, swollen and partially ruptured muscularis layer, vacuolated submucosa, damaged mucosa and distended and coalesced villi. The principal changes in the gills included vacuolated and disrupted epithelial cells and disorganized gill filaments, shrunken and oedamatous distensions in the primary lamellae, swollen, shortened and coalesced secondary lamellae and distorted and disintegrated gill arches. The changes in the liver included compactly or loosely arranged hepatic cells, reduced or swollen hepatic artery, disintegrated hepatic vein, blood coagulated portal vein, dilated and swollen central vein and dispersed sinusoids. The most toxic extract for P. pinnata was the leaf extract and for C. viscosum was the seed extract in three organs. Among the extracts of three plant parts (seed, leaf and bark) P. pinnata was found to be more toxic than C. viscosum. J. Asiat. Soc. Bangladesh, Sci. 39(1): 105-115, June 2013 DOI: http://dx.doi.org/10.3329/jasbs.v39i1.16039

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Toxic response of three predatory fishes to four indigenous plant seed extracts

Chittagong University Journal of Biological Sciences ◽

10.3329/cujbs.v6i1-2.17079 ◽

2013 ◽

Vol 6 (1-2) ◽

pp. 27-39

Author(s):

Munira Nasiruddin ◽

Mohammad Ali Azadi ◽

Tania Rahman ◽

Mst Ashrafunnessa

Keyword(s):

Indigenous Plants ◽

Anabas Testudineus ◽

Plant Seed ◽

Toxic Response ◽

Brassica Hirta ◽

Differential Toxicity ◽

Indigenous Plant ◽

Predatory Fishes ◽

Medium Toxicity ◽

Seed Extracts

Toxicity of seed extracts of four indigenous plants, Luffa acutangula (Roxb.), Areca catechue (Linn.), Brassica nigra (Linn. Koch.) and Brassica hirta (Monech) was tested on three predatory fishes- Heteropneustes fossilis (Bloch), Anabas testudineus (Bloch) and Channa punctatus (Bloch) under normal laboratory conditions. Due to the toxic effect of different seed extracts and concentrations, mortality rate varied. It also varied from species to species. On the basis of LC50 values of absolute ethyl alcohol extracts, L. acutangula seed extracts were more toxic for H. fossilis and A. testudineus. A. catechue seed extracts were of medium toxicity for all the experimental fishes. B. nigra seed extracts were less toxic whereas B. hirta seed extracts showed differential toxicity being more toxic for C. punctatus, medium for A. testudineus and less toxic for H. fossilis. The susceptibility pattern also varied in the three fish species. In case of L. acutangula, A. catechue and B. nigra seed extracts, the susceptibility was in the same order: H. fossilis > A. testudineus > C. punctatus but the effect of B. hirta seed extracts was reverse. To determine the ichthyotoxicity of the four seed extracts on the three predatory fishes, the present study was undertaken. DOI: http://dx.doi.org/10.3329/cujbs.v6i1-2.17079 The Chittagong Univ. J. B. Sci.,Vol. 6(1&2):27-39, 2011

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Precipitation Reactions Between Components of Plant Tissue Extracts

Functional Plant Biology ◽

10.1071/pp9750533 ◽

1975 ◽

Vol 2 (4) ◽

pp. 533 ◽

Cited By ~ 4

Author(s):

MA Jermyn

Keyword(s):

Concanavalin A ◽

Carbohydrate Binding ◽

Exact Nature ◽

Plant Seeds ◽

Tissue Extracts ◽

General Inhibitor ◽

Precipitation Reactions ◽

Leguminous Seeds ◽

Gel Diffusion ◽

Seed Extracts

Precipitation lines are observed on gel-diffusion plates between many pairs of seed extracts. Interactions involving Canavalia ensiformis depend upon concanavalin A, and a number of extracts from other plant seeds contain a precipitant that mimics that lectin. Methyl a-D-mannopyranoside is a general inhibitor of the precipitation phenomenon and some form of protein-carbohydrate binding seems to be involved in all precipitations, although the exact nature of the macromolecules taking part is obscure. For the leguminous seeds Phaseolus vulgauis, Vicia faba and Cajanus cajan, precipitation reactions occur between extracts of cotyledons and extracts of tissues of the parent plants, even of the testa of the seeds. The nature of these reactions appears to be the same as those of the interspecies ones. Both types of reaction may be examples of ways in which plant cells recognize self from non-self. The material in P. vulgaris that reacts with concanavalin A is a group of globulin-like glycoproteins (5 % carbohydrate), heterogeneous in both charge and molecular weight but similar in overall amino acid analysis.

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