scholarly journals Plant Regeneration from Leaf Explants of Plumbago

1970 ◽  
Vol 19 (1) ◽  
pp. 79-87 ◽  
Author(s):  
M. Gopalakrishnan ◽  
B. Janarthananm ◽  
G. Lakshmi Sai ◽  
T. Sekar
Keyword(s):  
Average Length ◽  
Leaf Explants ◽  
Basal Medium ◽  
Shoot Elongation ◽  
Mass Propagation ◽  
Plumbago Rosea ◽  
Half Strength ◽  
Maximum Root ◽  
Important Medicinal Plant

Leaf explants of Plumbago rosea L. an important medicinal plant inoculated on MS supplemented with 6.66 mM BAP and 2.69 mM NAA  produced numerous (105 ± 0.3) shootlets with an average length of 3.1 ± 0.0 cm. Small shootlets were transferred to shoot elongation medium supplemented with 1.11 mM BAP plus 1.44 mM GA3. The elongated shootlets transferred to half strength MS basal medium without any plant growth regulator produced 4.3 ± 0.2 rootlets per plants with average root length of 4.0 ± 0.0 cm after 25 days of culture. Rooted plantlets were transferred for hardening showed 80 - 90 per cent of plantlets success-fully established in the field. Potentially more than 50,000 plantlets could be produced within five subcultures from without callus phase obtained from leaf explant. Maximum root differentiation from leaf explants was obtained on MS supplemented with 5.38 mM IBA. The roots developed by the above method is an alternative for the controlled production of secondary metabolites. Key words:  In vitro culture, leaf explants, mass propagation, Plumbago rosea D.O.I. 10.3329/ptcb.v19i1.4989 Plant Tissue Cult. & Biotech. 19(1): 79-87, 2009 (June)

scholarly journals In Vitro Regeneration of Phyllanthus amarus Schum. and Thonn.: An Important Medicinal Plant

Our Nature ◽  
1970 ◽  
Vol 7 (1) ◽  
pp. 110-115 ◽  
Author(s):  
A. Sen ◽  
M.M. Sharma ◽  
D. Grover ◽  
A. Batra
Keyword(s):  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Phyllanthus Amarus ◽  
Regenerated Plants ◽  
Half Strength ◽  
Important Medicinal Plant

An efficient in vitro plant regeneration protocol was developed for the medicinally potent plant species Phyllanthus amarus Schum. and Thonn. (Euphorbiaceae) using nodal segment as explant. Maximum multiplication of shoots (15.275±0.96) was achieved on Murashige and Skoog’s medium supplemented with BAP (0.5 mg/l) after 3-4 weeks of inoculation. The shoots were separated from cluster and subcultured for their elongation on the same medium. In vitro flowering was also observed on the elongated shoots after 3–4 weeks of sub culturing on the shoot elongation medium. In vitro rooting was obtained on half strength MS medium supplemented with IBA (0.5 mg/l).  Regenerated plants were successfully hardened and acclimatized, 80 % of plantlets survived well under natural conditions after transplantation.Key words: In vitro regeneration, multiple shoots, nodal segments, Phyllanthus amarusDOI: 10.3126/on.v7i1.2557Our Nature (2009) 7:110-115

scholarly journals Micropropagation of Eclipta alba (Linn.) Hassk- a Valuable Medicinal Herb

1970 ◽  
Vol 43 (2) ◽  
pp. 215-222 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Farhana Afroz ◽  
Laila Shamroze Bari ◽  
John Liton Munshi ◽  
Miskat Ara Akhter Jahan ◽  
...  
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Mass Propagation ◽  
Eclipta Alba ◽  
Half Strength ◽  
Regenerated Plantlets

A protocol was established for mass propagation of a valuable medicinal herb, Eclipta alba (Linn.) Hassk (Asteraceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mgl-1 BAP + 0.1 mgl-1 NAA, in which 94% of the explants produced 18 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 26 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mgl-1 IBA +1.0 mgl-1 NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Eclipta alba, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization   DOI: 10.3329/bjsir.v43i2.965 Bangladesh J. Sci. Ind. Res. 43(2), 215-222, 2008 

scholarly journals CALLUS INDUCTION IN SOMATIC TISSUES OF PRUNUS PERSICA L.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 698c-698
Author(s):  
Veronique Declerck ◽  
Schuyler S. Korban
Keyword(s):  
Growth Regulators ◽  
Large Scale ◽  
Prunus Persica ◽  
Carbon Sources ◽  
Leaf Explants ◽  
Basal Medium ◽  
In Vitro Shoots ◽  
Somatic Tissues ◽  
Half Strength

Leaf segments of Prunus persica L. (peach) collected from greenhouse-grown plants and from micropropagated shoots were cultured on a basal medium containing half-strength Murashige and Skoog (MS), Staba vitamins, sucrose (30 g/1) and agar (6.5 g/l); medium adjusted to pH 5.6. The influence of 6 different growth regulators at 3 concentrations (5, 10, 15 μM) were investigated using leaf explants from proliferating shoots of 'Elberta Queen' peach. With thidiazuron (TDZ), compact and multiple green calli were obtained; with benzyladenine and zeatin, lower numbers of small sized calli were obtained; with kinetin, no callus development was observed. Among auxin treatments, both Dicamba and 2,4-D resulted in friable white and yellow calli. Most of the calli produced in all treatments were formed along the cut margins of the explants. In an another experiment, leaf explants of' Bellaire' (greenhouse) and `Elberta Queen' (in vitro shoots) were used to determine the influence of a large scale concentration of TDZ (3 to 23 |iM). Explants from greenhouse and in vitro leaves resulted in higher levels of callus development at TDZ concentrations of 8-13 μM. Higher TDZ levels resulted in necrosis of leaf explants. The-influence of different carbon sources on callogenesis was investigated. We observed more green and compact calli with glucose than with sucrose and fructose at 100 mM. The influence of the glucose at 10 different concentrations (30 to 300 mM) was also investigated.

scholarly journals In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

2012 ◽  
Vol 47 (3) ◽  
pp. 297-302
Author(s):  
AKMS Hassan ◽  
F Haque ◽  
MAA Jahan ◽  
SK Roy
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Plumbago Zeylanica ◽  
Half Strength ◽  
Regenerated Plantlets

An efficient protocol was developed for in vitro mass propagation of an important medicinal shrub, Plumbago zeylanica L., (Plumbaginaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 86.4% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooted culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. DOI: http://dx.doi.org/10.3329/bjsir.v47i3.13063 Bangladesh J. Sci. Ind. Res. 47(3), 297-302 2012

scholarly journals Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

2017 ◽  
Vol 5 (2) ◽  
pp. 15-26 ◽  
Author(s):  
Raihan I Raju ◽  
Shyamal K Roy
Keyword(s):  
In Vitro Culture ◽  
Basal Medium ◽  
Nodal Segments ◽  
Garden Soil ◽  
Root Induction ◽  
Ms Medium ◽  
Mass Propagation ◽  
Surface Sterilization ◽  
Half Strength

Protocol for mass propagation of Bambusa bamboos (L.) Voss was developed through in vitro culture. Nodal segments containing pre-existing axillary bud, after surface sterilization, were inoculated on liquid Murashige and Skoog’s (MS) basal medium containing different concentrations and combinations of cytokinins (BAP, TDZ and Kn). The highest direct shoot induction (90%) was obtained in the MS liquid medium supplemented with 2.0 mg/l BAP and 1.0 mg/l TDZ with maximum average number of shoots (3.14 ± 0.06) per explant. Highest shoot multiplication (16.58 ± 0.24 shoots per culture) with highest average shoot length (9.21 ± 0.13 cm) was obtained when in vitro raised shoots were cultured in gelrite gelled MS medium in conjunction with 2.0 mg/l BAP and 1.0 mg/l TDZ. Incorporation of 10% coconut water with 4% sucrose in the above mentioned medium resulted satisfactory shoot growth and development with an average 26.7 ± 0.60 shoots per culture. For root induction, in vitro raised shoots were divided into clumps of 4-5 shoots in each clump and transferred onto both liquid and gelled half-strength MS medium containing different concentrations and combinations of auxins (IBA and NAA). Maximum rooting (86.67%) was achieved in half-strength of MS medium fortified with 2.5 mg/l IBA and 2.5 mg/l NAA with an average 8.72 ± 0.42 root per shoot. The rooted plantlets were then transferred to polybags containing garden soil, sand and compost mixture with 1:1:1 ratio. After a month the hardened plantlets were then transferred to the larger pots containing garden soil and compost with 1:1 ratio for sufficient growth and finally transplanted to the field. In this process, the highest 100% survivability was recorded from well-established rooted plantlets. The regenerated plants showed well developed root and shoot systems in field condition.Jahangirnagar University J. Biol. Sci. 5(2): 15-26, 2016 (December)

scholarly journals In Vitro Mass Propagation of Heliotropium indicum L., using Apical and Axillary Bud Explants

1970 ◽  
Vol 45 (1) ◽  
pp. 69-74 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Nadira Begum ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Axillary Buds ◽  
Mass Propagation ◽  
Half Strength ◽  
Heliotropium Indicum ◽  
Regenerated Plantlets

A consequency was obtained for mass propagation of a valuable ayurvedic medicinal herb, Heliotropium indicum Linn. (Boraginaceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l GA3, in which 92% of the axillary buds explants produced 12 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 18 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Heliotropium indicum; Medicinal plant; Shoot proliferation; Micropropagation; Acclimatization. DOI: 10.3329/bjsir.v45i1.5185 Bangladesh J. Sci. Ind. Res. 45(1), 69-74, 2010

scholarly journals In Vitro Mass Propagation of Mimosa pudica L., Using Shoot Tip and Nodal Explants

1970 ◽  
Vol 45 (2) ◽  
pp. 95-100 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Rebeka Sultana ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Mimosa Pudica ◽  
Mass Propagation ◽  
Half Strength ◽  
Proliferation In Vitro

An efficient protocol was established for in vitro mass propagation of a valuable medicinal shrubby plant, Mimosa pudica Linn., from shoot tip and nodal explants. Optimum in vitro shoot induction was observed from nodal explants on MS basal medium supplemented with 1.5 mg/l BAP + 0.5 mg/l NAA, in which 88.2% of the explants produced 9 shoots per culture within 3-4 weeks. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 20.4 ± 1.20 shoots per culture within 12 weeks. The healthy in vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Mimosa pudica; Medicinal plant; Shoot proliferation; In vitro mass propagation; Acclimatization DOI: 10.3329/bjsir.v45i2.5704Bangladesh J. Sci. Ind. Res. 45(2), 95-100, 2010

scholarly journals Plant Regeneration from Leaf Derived Callus of Stevia rebaudiana Bertoni

1970 ◽  
Vol 19 (2) ◽  
pp. 133-141 ◽  
Author(s):  
B. Janarthanam ◽  
M. Gopalakrishnan ◽  
G. Lakshmi Sai ◽  
T. Sekar
Keyword(s):  
Plant Regeneration ◽  
Plant Tissue ◽  
Growth Response ◽  
Average Length ◽  
Leaf Explants ◽  
Basal Medium ◽  
Stevia Rebaudiana ◽  
Stevia Rebaudiana Bertoni ◽  
Juvenile Leaf ◽  
Half Strength

Juvenile leaf explants of Stevia rebaudiana Bertoni produced maximum callus than the nodal explants  cultured on MS containing 11.31 mM 2, 4-D and 2.22 mM BAP. Callus transferred to MS supplemented with 4.44 mM BA and 1.34 mM NAA showed better growth response and produced 14.0 ± 1.0 shoots with an average length of 5.6 ± 0.1 cm after 28 days. All plantlets produced profuse rooting within 25 days after transfer to half strength of MS basal medium supplemented with 2.46 mM IBA. Rooted plantlets were transferred for hardening, with 90% of plantlets successfully established in the field.  Key words: Stevia rebaudiana, Leaf explant, callus culture, micropropagation D.O.I. 10.3329/ptcb.v19i2.5430 Plant Tissue Cult. & Biotech. 19(2): 133-141, 2009 (December)

scholarly journals Development of a profused In vitro shoot multiplication using leaf explants of Bacopa monnieri (L.) Pennell

2020 ◽  
pp. 14-17
Author(s):  
Sape Subba Tata
Keyword(s):  
Medicinal Plant ◽  
Leaf Explants ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Bacopa Monnieri ◽  
Optimum Concentration ◽  
Ms Medium ◽  
Efficient Regeneration ◽  
Important Medicinal Plant

Bacopa monnieri (L.) Pennell is an important medicinal plant used for the preparation of medhyarasayan (rasayana). Leaf explants of field grown young plants of B. monnieri was used to establish an efficient regeneration protocol with cytokinin (BAP) and auxin (IAA). The highest multiplication, i.e. (220 shoots/leaf, a cumulative of 2200 shoots from 10 explants) were noticed after 45 days of culture in MS medium supplemented with BAP(1.5mg/L) and IAA(0.5mg/L). The optimum concentration of growth regulator for shoot elongation and rooting was recorded in MS+GA3(0.25mg/L) and MS+IBA(1.5mg/L) respectively. The rooted plantlets were successfully established in green house conditions.

scholarly journals Adventitious Shoot Regeneration from In Vitro Leaves of Formosan Sweetgum (Liquidambar formosana L.)

HortScience ◽  
2007 ◽  
Vol 42 (3) ◽  
pp. 721-723 ◽  
Author(s):  
L. Xu ◽  
G.F. Liu ◽  
M.Z. Bao
Keyword(s):  
Shoot Regeneration ◽  
Adventitious Shoots ◽  
Leaf Explants ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Elongation ◽  
Naphthalene Acetic Acid ◽  
Regeneration Rate ◽  
High Regeneration

Plantlets were regenerated from in vitro-grown leaf explants of five genotypes of Liquidambar formosana on WPM basal medium supplemented with different concentrations of TDZ and NAA. With the addition of 0.27 μm NAA, regeneration efficiency was increased by 2- to 4-fold over that with TDZ alone. Lower concentrations of TDZ (0.45–2.27 μm) were beneficial for regenerating shoot clusters. Four genotypes (P2, P6, P9, and P11) showed high regeneration rates (up to 90%), whereas genotype P13 showed a low capability for shoot regeneration on all media tested (<35%). For all five genotypes, the optimum medium for inducing adventitious shoots was WPM supplemented with 1.14 μm TDZ and 0.27 μm NAA, on which regeneration rate ranged from 72.6% to 89.5% and adventitious shoot clusters per regenerating leaf explant ranged from 2.63 to 3.11 in four genotypes (P2, P6, P9, and P11), while for P13, the regeneration rate and number of shoot clusters per regenerating explant were 23% and 1.39, respectively. Transfer of shoot clusters to WPM basal medium containing 0.54 μm NAA, 2.22 μm BA, and 1.44 μm GA3, resulted in shoot elongation. All the elongated shoots were rooted on WPM supplemented with 9.84 μm IBA, and plantlets were transplanted to soil successfully. Chemical names used: 6-benzyladenine (BA), gibberellic acid (GA3), indole-3-butyric acid (IBA), 1-naphthalene acetic acid (NAA), plant growth regulator (PGR), thidiazuron (TDZ), woody plant medium (WPM).

Sign in / Sign up

Export Citation Format

Share Document