scholarly journals Development of a profused In vitro shoot multiplication using leaf explants of Bacopa monnieri (L.) Pennell

2020 ◽  
pp. 14-17
Author(s):  
Sape Subba Tata
Keyword(s):  
Medicinal Plant ◽  
Leaf Explants ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Bacopa Monnieri ◽  
Optimum Concentration ◽  
Ms Medium ◽  
Efficient Regeneration ◽  
Important Medicinal Plant

Bacopa monnieri (L.) Pennell is an important medicinal plant used for the preparation of medhyarasayan (rasayana). Leaf explants of field grown young plants of B. monnieri was used to establish an efficient regeneration protocol with cytokinin (BAP) and auxin (IAA). The highest multiplication, i.e. (220 shoots/leaf, a cumulative of 2200 shoots from 10 explants) were noticed after 45 days of culture in MS medium supplemented with BAP(1.5mg/L) and IAA(0.5mg/L). The optimum concentration of growth regulator for shoot elongation and rooting was recorded in MS+GA3(0.25mg/L) and MS+IBA(1.5mg/L) respectively. The rooted plantlets were successfully established in green house conditions.

scholarly journals In Vitro Propagation of Digitalis trojana Ivanina., an Endemic Medicinal Plant of Turkey

2020 ◽  
Author(s):  
Nurşen Çördük ◽  
Cüneyt Aki
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
In Vitro Propagation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Northwestern Turkey ◽  
Helen Of Troy ◽  
Endemic Medicinal Plant

Digitalis trojana Ivanina is a member of the Plantaginaceae family and known by its common name, Helen of Troy foxglove. It is perennial endemic to Çanakkale and Balıkesir, northwestern Turkey. In order to develop an efficient shoot regeneration protocol, the leaf explants of D. trojana were cultured on Murashige and Skoog (MS) medium containing 6-benzyl adenine (0.1, 0.5, 1.0, 3.0, 5.0 mg/L) and α-naphthalene acetic acid (0.1, 0.5, 1.0 mg/L), 3% (w/v) sucrose and 0.8% (w/v) agar. The highest number of regenerated shoots was obtained from leaf explants that were cultured on MS medium with 3.0 mg/L BA+0.1 mg/L NAA. Regenerated shoots were rooted on MS medium without plant growth regulators. Rooted plants (2–3 cm) were separately transferred to pots containing a mixture of peat and perlite (2:1 v/v) and acclimatized successfully in a growth chamber.

scholarly journals Micropropagation of PLUCHEA LANCEOLATA (Oliver & Hiern.) Using Nodal Explant

2014 ◽  
Vol 22 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Mafatlal M. Kher ◽  
Dimpal Joshi ◽  
Sureshkumar Nekkala ◽  
M. Nataraj ◽  
Dharmesh P. Raykundaliya
Keyword(s):  
Medicinal Plant ◽  
Salt Concentration ◽  
Nodal Explants ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Nodal Explant ◽  
Pluchea Lanceolata ◽  
Asteraceae Family ◽  
Important Medicinal Plant

AbstractPluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. lanceolata using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962) - MS medium supple–mented with 6-benzylaminopurine (BAP), kinetin (Kin), thidiazuron (TDZ) and 2iP (2-isopentenyladenine) at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3). The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ). In vitro raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.

scholarly journals Histological studies of in vitro regeneration induced in leaf explants of Nymphoides cristatum (Roxb) O.Kuntze – An aquatic medicinal plant

1970 ◽  
Vol 3 ◽  
Author(s):  
Mallapa Hanumanthu Niranjan ◽  
Mysore Shankar Sudarshana
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Free Medium ◽  
Histological Studies ◽  
Leaf Histology ◽  
Benzyl Amino Purine

The objective of this work was to study the histological events related to the regeneration process of a medicinal plant, Nymphoides cristatum (Roxb). Leaf explants were cultured on MS medium supplemented with 0.5 mgl-1 of 6-benzyl amino purine (BAP). About 90% of explants gave rise to shoots after 15 days of incubation. The histological studies showed that the regeneration originated directly from parenchymatous cells and direct organogenesis after 20 days of culture could be observed. Buds and roots were found completely differentiated after 40 days of culture and number of shoots per explants was 30. Micorshoots were rooted in hormone - free medium and the plants obtained grew in artificial pond under green house conditions. Key words: Leaf, Histology, in vitro regeneration, Nymphoides cristatum.  DOI: 10.3126/ijls.v3i0.2370

scholarly journals In vitro propagation of Nanorrhinum ramosissimum (Wall.) Betsche: A traditionally important medicinal plant

2021 ◽  
Vol 48 (3) ◽  
Author(s):  
Jyotsna Sharma ◽  
◽  
Anuja Koul ◽  
Savita Sharma ◽  
Raju Shankarayan ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Shoot Organogenesis ◽  
Germplasm Conservation ◽  
Ms Medium ◽  
In Vitro Plantlets ◽  
Half Strength ◽  
Shoot Tip Explants ◽  
Indole 3 Acetic Acid ◽  
Important Medicinal Plant

An efficient micropropagation protocol facilitates successful conservation and improvement of Nanorrhinum ramosissimum (Wall.) Betsche by biotechnological means. Shoot tip explants exhibited optimal organogenic response when inoculated on half-strength(1/2) Murashige and Skoog (MS) medium supplemented with kinetin (KN) and indole-3-acetic acid (IAA) (0.5 mg/L each). Shoot organogenesis was further enhanced when the multiplication medium was fortified with dextrose (1%) (2.6 shoots/explant; 7.9 cm shoot length). The regenerated shoots formed roots; however, the best rooting frequency (87%) was achieved on half-strength MS medium containing only IAA (0.5 mg/L). Four-week-old in vitro plantlets were acclimatized with 95% survival under greenhouse conditions. The regeneration protocol developed in this study can be utilized for germplasm conservation of this elite traditional medicinal plant.

scholarly journals (281) Micropropagation of Trifoliate Orange Rootstock (Poncirus trifoliate Raf.)

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1052C-1052 ◽  
Author(s):  
Abdelrahman Al-Wasel
Keyword(s):  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
Trifoliate Orange ◽  
Mature Trees ◽  
Poncirus Trifoliate

In vitro propagation of trifoliate orange rootstock (Poncirus trifoliate Raf.) was achieved using axillary buds taken from new flushes of mature trees and then cultured on Murashige and Skoog medium (MS). The addition of growth regulators [0.5 mg·L-1 gibberellic acid (GA3) or 0.1 mg·L-1 6-benzyladenine (BA) and 0.1 mg·L-1 indol-3-butyric acid (IBA)] were necessary to promote bud breakage and shoot elongation. Shoot proliferation was induced on MS medium supplemented with various levels of BA (0.0, 0.5, 1.0, 1.5, and 2.0 mg·L-1) and α-naphthalene acetic acid (NAA) (0.0, 0.1, and 0.5 mg·L-1). Maximal shoot multiplication (9.3 shoots/explant) and elongation (2.3 cm) occurred on media containing either 1.0 mg·L-1 BA alone or with 0.1 mg·L-1 NAA. Shoots rooted better and gave high root number (7.6 roots/shoot) and long roots (5.4 cm) when cultured on a liquid MS medium provided by 0.1 mg·L-1 NAA. Rooted shoots were successfully established in soil (≥90%).

scholarly journals Micropropagation Of Merremia Quinquefolia (L.) Hallier F. From Nodal Explants

2015 ◽  
Vol 23 (1) ◽  
pp. 13-16
Author(s):  
Mafatlal M. Kher ◽  
M. Nataraj ◽  
Hettal D. Parmar ◽  
Hasmatbanu Buchad
Keyword(s):  
Shoot Multiplication ◽  
Nodal Explants ◽  
Ms Medium ◽  
Mineral Salts ◽  
Single Node ◽  
The Family ◽  
Sedative Effects ◽  
Bud Breaking ◽  
Important Medicinal Plant

AbstractMerremia quinquefolia, is an important medicinal plant of the family Convolvulaceae known for its vasoconstrictor, uterotonic, neurohormonic, sympathicolytic and sedative effects. In the present investigation effect of cytokinins 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ), at concentrations 1.0, 2.0, 3.0, 4.0 and 5.0 mg·dm−3 on in vitro shoot multiplication from nodal explants of M. quinquefolia was evaluated. Bud breaking and emergence of shoots started within 10-15 days of inoculation in all media containing cytokinin. Murashige and Skoog (MS) medium supplemented with 4.0 mg·dm−3 BAP resulted in maximum number of shoots from single node within 45 days. In vitro raised shoots were successfully rooted on ½ mineral salts of MS medium with 3% sucrose supplemented with 2.0 mg·dm−3 indole-3-butyric acid (IBA). This is the first report on in vitro propagation of Merremia quinquefolia. This study can be useful for development of micropropagation protocols for related taxa.

scholarly journals Micropropagation, Micromorphological Studies, andIn VitroFlowering inRungia pectinataL.

Scientifica ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Mahipal S. Shekhawat ◽  
M. Manokari ◽  
C. P. Ravindran
Keyword(s):  
Tissue Culture ◽  
Shoot Multiplication ◽  
Developmental Changes ◽  
Bud Break ◽  
Ms Medium ◽  
Half Strength ◽  
Indole 3 Acetic Acid ◽  
Culture Protocol ◽  
Important Medicinal Plant

A tissue culture protocol was developed for an important medicinal plantRungia pectinataL. in the present study. Nodal shoots were used as explants and surface-sterilized with 0.1% HgCl2solution. Murashige and Skoog (MS) medium was used to establish the cultures ofR. pectinata. The bud break was reported on MS medium supplemented with 1.0 mg L−16-benzylaminopurine (BAP). About 98% response was observed with this media combination and maximum 3.2 shoots per explant with 4.3 cm length were recorded. The shoots were further multiplied using MS medium augmented with 0.5 mg L−1each of BAP and kinetin (Kin) + 0.1 mg L−1indole-3 acetic acid (IAA). Maximum 13.2 shoots per explant with 5.2 cm length were observed. All the shoots were rooted (4.9 roots per shoot with 3.5 cm length) on half strength MS medium fortified with 2.0 mg L−1indole-3 butyric acid (IBA).In vitroflowering was induced from the shoots on half strength MS medium supplemented with same concentrations and combinations of growth regulators used for shoot multiplication under 12/12 hr light/dark photoperiod. The plantlets were hardened in the greenhouse for two months and finally transferred to the field. The foliar micromorphological studies revealed the developmental changes in stomata, vein density, and trichomes during the culture of shoots underin vitroconditions.

scholarly journals Study on in vitro propagation of Giao co lam (Gynostemma pentaphyllium Thunb.)

2018 ◽  
Vol 17 (05) ◽  
pp. 84-92
Author(s):  
Trinh L. D. Ho
Keyword(s):  
Leaf Explants ◽  
Shoot Multiplication ◽  
Young Leaf ◽  
Ms Medium ◽  
Nodal Explant ◽  
Coconut Fiber ◽  
Average Percentage ◽  
Endangered Medicinal Herb ◽  
Multiplication Score

Giao co lam (Gynostemma pentaphyllium Thunb.) is a traditional medicine plant and endangered species in Vietnam. The research was carried out to establish the plant propagation for the purpose of conserving and exploiting this endangered medicinal herb. The young leaf and nodes of Giao co lam in vitro were used as explants in the study to evaluate the factors influencing the multiplication. Young leaf explants were excised and cultured in MS medium with TDZ from 0.1 to 1 mg/L. After 10 weeks of culture, new shoots came out from their explants and the MS medium containing TDZ 0,7 mg/L gave the highest shoots (12.89 shoots/explant) with the average percentage of 74.67%. When nodal explants were cultured on MS supplemented with BA at a concentration of 0.3 to 1.5 mg/L and IBA 0.5 mg/L. After 6 weeks of culture, explants on MS medium supplemented with BA 1 mg/L and IBA 0,5 mg/L gave the highest shoots (7.39 shoots/explant) and their average percentage was 83.33%. In comparison to the nodal explant medium in combination with BA (0.5 to 3 mg/L) and NAA 0.2 mg/L for 4 weeks of culture, the best rapid shoot multiplication score was 3.67 times with MS + BA 1.5 mg/L + NAA 0.2 mg/L as compared to MS + BA 1.0 mg/L + IBA 0.5 mg/L. Suitable medium for rooting was MS + 0.5 mg/L IBA with root shoots at 97.33%, average roots at 5.29 roots/shoot after 4 weeks. On organic substrat, 70% coconut fiber and 30% composted cow manure gave the highest survival rate of 91.33%. The plants grew and developed well during the nursery stage.

scholarly journals Plant Regeneration from Leaf Explants of Plumbago

1970 ◽  
Vol 19 (1) ◽  
pp. 79-87 ◽  
Author(s):  
M. Gopalakrishnan ◽  
B. Janarthananm ◽  
G. Lakshmi Sai ◽  
T. Sekar
Keyword(s):  
Average Length ◽  
Leaf Explants ◽  
Basal Medium ◽  
Shoot Elongation ◽  
Mass Propagation ◽  
Plumbago Rosea ◽  
Half Strength ◽  
Maximum Root ◽  
Important Medicinal Plant

Leaf explants of Plumbago rosea L. an important medicinal plant inoculated on MS supplemented with 6.66 mM BAP and 2.69 mM NAA  produced numerous (105 ± 0.3) shootlets with an average length of 3.1 ± 0.0 cm. Small shootlets were transferred to shoot elongation medium supplemented with 1.11 mM BAP plus 1.44 mM GA3. The elongated shootlets transferred to half strength MS basal medium without any plant growth regulator produced 4.3 ± 0.2 rootlets per plants with average root length of 4.0 ± 0.0 cm after 25 days of culture. Rooted plantlets were transferred for hardening showed 80 - 90 per cent of plantlets success-fully established in the field. Potentially more than 50,000 plantlets could be produced within five subcultures from without callus phase obtained from leaf explant. Maximum root differentiation from leaf explants was obtained on MS supplemented with 5.38 mM IBA. The roots developed by the above method is an alternative for the controlled production of secondary metabolites. Key words:  In vitro culture, leaf explants, mass propagation, Plumbago rosea D.O.I. 10.3329/ptcb.v19i1.4989 Plant Tissue Cult. & Biotech. 19(1): 79-87, 2009 (June)

PLANT REGENERATION OF KAMCHATIC PLANTAIN (PLANTAGO CAMTSCHATICA LINK) THROUGH CALLUS INDUCTION

2017 ◽  
Vol 19 (3) ◽  
pp. 41-48
Author(s):  
Ay N.V. ◽  
Duy M.V. ◽  
Baatartsogt O. ◽  
Altantsetseg Kh. ◽  
Enkhchimeg V.
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Leaf Explants ◽  
Stage Iv ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Development Stage ◽  
High Survival ◽  
Ms Medium

In vitro seedling offspring of Plantago camtschatica Link was investigated regarding induction of somatic embryogenesis in petiole/leaf explants from shoot tissue and shoot proliferation. The aim of study was to investigate the medium supplemented with suitable concentration of plant growth regulators in order to induce somatic embryogenesis, plant regeneration and shoot multiplication. The results showed that: (i) Petiole/young leaf of immature stem induced the highest ratio of calli induction and compact calli formation on MS medium supplemented with 1 mgL-1 2,4-D and 0.5 mgL-1 BA; (ii) From created calli, somatic embryogenesis could be induced on MS medium supplemented with 1 mgL-1 TDZ or 1 mgL-1 TDZ and 0.5 mgL-1 NAA; (iii) MS medium supplemented with 5-7 mgL-1 BA shown the most effective on shoot development stage; (iv) Rooting of shoot was the best on 1/2 solid MS medium with activated charcoal (2 gL-1), and 0.5-4 mgL-1 NAA; and (v) acclimatization of micropropagated plants could be planted in plastic pots containing a mixture of decayed straw : rice husk ashes, (1:1, v/v), sand : soil (1:1, v/v) or soil, showed a high survival rate and most seedlings grew normally.

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