scholarly journals Plant Regeneration from Leaf Derived Callus of Stevia rebaudiana Bertoni

1970 ◽  
Vol 19 (2) ◽  
pp. 133-141 ◽  
Author(s):  
B. Janarthanam ◽  
M. Gopalakrishnan ◽  
G. Lakshmi Sai ◽  
T. Sekar
Keyword(s):  
Plant Regeneration ◽  
Plant Tissue ◽  
Growth Response ◽  
Average Length ◽  
Leaf Explants ◽  
Basal Medium ◽  
Stevia Rebaudiana ◽  
Stevia Rebaudiana Bertoni ◽  
Juvenile Leaf ◽  
Half Strength

Juvenile leaf explants of Stevia rebaudiana Bertoni produced maximum callus than the nodal explants  cultured on MS containing 11.31 mM 2, 4-D and 2.22 mM BAP. Callus transferred to MS supplemented with 4.44 mM BA and 1.34 mM NAA showed better growth response and produced 14.0 ± 1.0 shoots with an average length of 5.6 ± 0.1 cm after 28 days. All plantlets produced profuse rooting within 25 days after transfer to half strength of MS basal medium supplemented with 2.46 mM IBA. Rooted plantlets were transferred for hardening, with 90% of plantlets successfully established in the field.  Key words: Stevia rebaudiana, Leaf explant, callus culture, micropropagation D.O.I. 10.3329/ptcb.v19i2.5430 Plant Tissue Cult. & Biotech. 19(2): 133-141, 2009 (December)

scholarly journals A Rapid, High Frequency Regeneration of Justicia gendarussa Burm.f.

2011 ◽  
Vol 46 (2) ◽  
pp. 201-204 ◽  
Author(s):  
B Janarthanam ◽  
B Gayathri ◽  
E Sumath
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Growth Response ◽  
Average Length ◽  
Coconut Milk ◽  
Nodal Segments ◽  
Average Height ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
Half Strength

An efficient plant regeneration protocol was developed for Justicia gendarussa Burm.f. (Acanthaceae), an important medicinal shrub. Nodal segments grown on Murashige and Skoog (MS) medium containing 1.0 mg/l Benzyl adenine (BAP) with 10 % coconut milk showed better growth response and produced 10.5 ± 0.6 shoots per explant with an average length of 4.4 ± 0.3 cm after 35 days. Rooting of shoots was achieved on growth regulator free half strength MS medium produced 5.3 ± 0.25 cm roots with an average height of 4.8 ± 0.2 cm after 30 days. The rooted plantlets were transferred for hardening, 80 % of plants were successfully established in the field. Key words: Justicia gendarussa; Nodal segments; Regeneration; Coconut milk. DOI: http://dx.doi.org/10.3329/bjsir.v46i2.4388 Bangladesh J. Sci. Ind. Res. 46(2), 201-204, 2011

scholarly journals In vitro Plant Regeneration from Shoot tip Explants of Exacum travancoricum Beedi

1970 ◽  
Vol 20 (2) ◽  
pp. 113-118 ◽  
Author(s):  
B. Janarthanam ◽  
E. Sumathi Sumathi
Keyword(s):  
Plant Regeneration ◽  
Growth Response ◽  
Average Length ◽  
Shoot Tip ◽  
Average Height ◽  
In Vitro Plant Regeneration ◽  
Farm Yard Manure ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants of Exacum travancoricum, an endangered herb mainly known for ornamental purpose grown on MS with 4.44 μM BAP and 1.34 μM NAA showed better growth response and produced 29.3 ± 0.3 shoots per explant with an average length of 4.6 ± 0.1 cm after 35 days. Roots were induced after transfer to half strength of MS supplemented with 2.46 μM IBA produced 4.8 ± 0.62 roots with an average height of 3.6 ± 0.10 cm after 30 days. The rooted plantlets were transferred for hardening, 80 per cent of plantlets survived and resumed growth in the mixture of soil, vermiculite and farm yard manure (1 : 1 : 1). Key words: Exacum travancoricum; Shoot tip; Plant regeneration D.O.I. 10.3329/ptcb.v20i2.6889 Plant Tissue Cult. & Biotech. 20(2): 113-118, 2010 (December)

scholarly journals Rapid and Efficient Plant Regeneration from Nodal Explants of Artemisia annua L.

2012 ◽  
Vol 22 (1) ◽  
pp. 33-39 ◽  
Author(s):  
B. Janarthanam ◽  
P. Rashmi ◽  
E. Sumathi
Keyword(s):  
Plant Regeneration ◽  
Growth Response ◽  
Artemisia Annua ◽  
Average Length ◽  
Shoot Elongation ◽  
Nodal Explants ◽  
Average Height ◽  
Farm Yard Manure ◽  
Artemisia Annua L ◽  
Half Strength

An efficient plant regeneration protocol was developed for Artemisia annua, an aromatic medicinal herb. Nodal explants inoculated on MS supplemented with 4.44 ?M BAP showed better growth response and produced 116.2 ± 0.1 micro-shoots of an average length 1.9 ± 0.3 cm after 35 days culture. The cluster of small shootlets were cultured on shoot elongation medium supplemented with 1.44 ?M GA3 and 10% coconut milk (CM) showed shoot elongation up to 4.6 ± 0.7 cm. Roots were induced after transfer to half strength MS supplemented with 2.46 ?M IBA produced 14.3 ± 0.2 roots with an average height of 4.3 ± 0.53 cm after 30 days. The rooted plantlets were transferred for hardening, 80 per cent of plantlets survived and resumed growth in the mixture of soil, vermiculite and farm yard manure (1 :  1 : 1). DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11257 Plant Tissue Cult. & Biotech. 22(1): 33-39, 2012 (June)

scholarly journals Plant Regeneration from Leaf Explants of Plumbago

1970 ◽  
Vol 19 (1) ◽  
pp. 79-87 ◽  
Author(s):  
M. Gopalakrishnan ◽  
B. Janarthananm ◽  
G. Lakshmi Sai ◽  
T. Sekar
Keyword(s):  
Average Length ◽  
Leaf Explants ◽  
Basal Medium ◽  
Shoot Elongation ◽  
Mass Propagation ◽  
Plumbago Rosea ◽  
Half Strength ◽  
Maximum Root ◽  
Important Medicinal Plant

Leaf explants of Plumbago rosea L. an important medicinal plant inoculated on MS supplemented with 6.66 mM BAP and 2.69 mM NAA  produced numerous (105 ± 0.3) shootlets with an average length of 3.1 ± 0.0 cm. Small shootlets were transferred to shoot elongation medium supplemented with 1.11 mM BAP plus 1.44 mM GA3. The elongated shootlets transferred to half strength MS basal medium without any plant growth regulator produced 4.3 ± 0.2 rootlets per plants with average root length of 4.0 ± 0.0 cm after 25 days of culture. Rooted plantlets were transferred for hardening showed 80 - 90 per cent of plantlets success-fully established in the field. Potentially more than 50,000 plantlets could be produced within five subcultures from without callus phase obtained from leaf explant. Maximum root differentiation from leaf explants was obtained on MS supplemented with 5.38 mM IBA. The roots developed by the above method is an alternative for the controlled production of secondary metabolites. Key words:  In vitro culture, leaf explants, mass propagation, Plumbago rosea D.O.I. 10.3329/ptcb.v19i1.4989 Plant Tissue Cult. & Biotech. 19(1): 79-87, 2009 (June)

scholarly journals Indirect in vitro Regeneration of Viola canescens Wall. ex, Roxb. by using Leaf Calli

2018 ◽  
Vol 28 (2) ◽  
pp. 215-222 ◽  
Author(s):  
Arun Kumar Khajuria ◽  
NS Bisht
Keyword(s):  
Plant Regeneration ◽  
Natural Population ◽  
Plant Tissue ◽  
In Vitro Regeneration ◽  
Soil Condition ◽  
Medicinal Herb ◽  
Growth Chamber ◽  
Half Strength ◽  
Number Of Shoots

An efficient indirect plant regeneration protocol was developed for Viola canescens, an important medicinal herb used in broad spectra of diseases in number of folk medicines since aeon. Excessive use of this plant without any rehabilitating measure has led to decline its natural population. Present investigation reports the use of zeatin to regenerate the plant from the callus on MS following its acclimatization on the soil condition. Calli of the plant responded positively to zeatin and maximum number of shoots 13.07 ± 2.01 were obtained when 9.12 μM concentration of zeatin was used. Regenerated shoots were subsequently rooted with IBA on MS and half strength MS and showed maximum number of roots 14.13 ± 1.64 after 60 days when medium was fortified with 4.92 μM IBA, followed by transferring them to soil condition, acclimatization of the plantlet was carried in growth chamber and then finally to the field for their survival where it showed 80% survival. Plant Tissue Cult. & Biotech. 28(2): 215-222, 2018 (December)

scholarly journals Plant regeneration via somatic embryogenesis from immature leaves in Tetrapleura tetraptera (Schum. & Thonn.) Taub.

2011 ◽  
Vol 63 (4) ◽  
pp. 1135-1145 ◽  
Author(s):  
J.T. Opabode ◽  
O.A. Akinyemiju ◽  
O.O. Ayeni
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Leaf Explants ◽  
Basal Medium ◽  
Shoot Elongation ◽  
Leaf Petiole ◽  
Immature Leaf ◽  
Embryogenic Calluses ◽  
Tetrapleura Tetraptera ◽  
Secondary Embryos

Plant regeneration via somatic embryogenesis was assessed using immature leaf, petiole and apical meristem explants in Tetrapleura tetraptera. Somatic embryos were induced in the immature leaf using MS basal medium supplemented with 2,4-D and matured on MS basal medium containing BAP. Medium supplemented with 12 mg/l 2,4-D had the highest (43.1%) percentage of embryogenic calluses from immature leaf explants. Conversion of embryogenic callus to mature primary somatic embryo occurred in the medium that contained 1.2 mg/l BAP. Development of secondary embryogenic calluses to matured secondary embryos was highest (98.0%) in the medium with 0.4 mg/l BAP, while the highest average number of mature secondary embryos (6.0) was obtained in the same medium. Medium supplemented with 1.0 mg/l BAP and 0.5 mg/l IBA had the highest (38.7%) percentage of explants with shootbuds. The highest (18.1%) percentage of shoot elongation was obtained in medium with 1.0 mg/l BAP and 20 mg/l IBA. Shootbuds survived and produced roots on medium free of plant growth regulators. Shoots obtained on medium supplemented with 1.0 mg/l BAP and 20 mg/g IBA recorded the highest number of roots per plantlet (7.5) with no apparent morphological abnormality.

scholarly journals Plantlet regeneration from nodal explants of Ocimum citriodorum Vis.

2013 ◽  
Vol 47 (4) ◽  
pp. 433-436
Author(s):  
Balasundaram Janarthanam ◽  
Sumathi Ethiraj
Keyword(s):  
Growth Response ◽  
Average Length ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Average Height ◽  
Ms Medium ◽  
Farm Yard Manure ◽  
Multiple Shoot Induction ◽  
Half Strength ◽  
Indole 3 Acetic Acid

A protocol for multiple shoot induction and plant regeneration from nodal explants of Ocimum citriodorum has been developed. Nodal explants inoculated on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l Benzyl adenine (BAP) and 0.025 mg/l indole -3- acetic acid (IAA) showed better growth response (80%) and produced 15.2 ± 1.28 shoots per explant with an average length of 6.17 ± 0.29 cm after 35 days. Roots were induced after transfer to half strength MS medium supplemented with 0.5 mg/l Indole -3- butyric acid (IBA) produced 6.0 ± 1.0 roots with an average height of 4.9 ± 0.26 cm after 30 days. Plantlets with well developed root and shoot systems were successfully acclimated (80 %) and established in earthen pots containing mixture of soil, vermiculite and farm yard manure (1:1:1). Bangladesh J. Sci. Ind. Res. 47(4), 433-436, 2012 DOI: http://dx.doi.org/10.3329/bjsir.v47i4.8547

scholarly journals The Success Rate of Two Explant Types of Stevia (Stevia rebaudiana Bertoni) in Various Sterilant Formulas

2020 ◽  
Vol 3 (2) ◽  
pp. 92
Author(s):  
Riedha Kariena ◽  
Nofia Hardarani ◽  
Hilda Susanti
Keyword(s):  
Success Rate ◽  
Leaf Explants ◽  
Stevia Rebaudiana ◽  
Stevia Rebaudiana Bertoni ◽  
Randomized Design ◽  
Two Factors

This study aims to determine the effect of interaction between several sterilants and types of explants and determine the best interaction with stevia culture's success rate. This study was designed using a Factorial Complete Randomized Design with two factors and three replications. The first factor is sterilant formulas i.e: fungicide 3% + alcohol 70% + Bayclin 5%; fungicide 3% + bactericide 6% + 70% alcohol + Bayclin 5%; sublimate 0.1% + 70% alcohol + Bayclin 5%; and fungicide 3% + bactericide 6% + sublimate 0.1% + alcohol 70% + Bayclin 5%. The second factor is explant types, i.e., stevia nodes and leaves. The variables observed are the percentage of contamination (%), percentage of alive explants (%), and browning percentage.  The interaction between sterilants and explant types only had a significant effect on the percentage of alive explants. The best interactions of sterilant formulas and explant types on the percentage of alive explants are 0.1% sublimate + 70% alcohol + 5% Bayclin and leaf explants.

scholarly journals Plant Regeneration from Leaf Explants of the Medicinal Herb Wedelia chinensis

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 407
Author(s):  
Yung-Ting Tsai ◽  
Kin-Ying To
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Liver Toxicity ◽  
Leaf Explants ◽  
Basal Medium ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Average Percentage

Wedelia chinensis, belonging to the Asteraceae family, has been used in folk medicine in East and South Asia for the treatment of common inflammatory diseases and protection against liver toxicity. Previously, in vitro propagation through different tissue explants has been reported, including through nodal segments, axillary buds, and shoot tips, whereas leaf segments failed to proliferate. Here, we report on the in vitro propagation of W. chinensis by culturing young leaf explants in MS medium supplemented with 0.5 mg/L α-naphthaleneacetic acid (NAA), 0.75 mg/L thidiazuron (TDZ), 1 mg/L gibberellic acid (GA3), 3.75 mg/L adenine, 3% sucrose, and 0.8% agar at pH 5.8. Calli were observed in all explants derived from the youngest top two leaves, and the average percentage of shoot regeneration was 23% from three independent experiments. Then, several shoots were excised, transferred onto MS basal medium supplemented with 3% sucrose and 0.8% agar at pH 5.8, and cultured in a growth chamber for 1 to 2 months. Roots were easily induced. Finally, plantlets carrying shoots and roots were transferred into soil, and all of them grew healthily in a greenhouse. No morphological variation was observed between the regenerated plantlets and the donor wild-type plants. In addition, we also established root cultures of W. chinensis in culture medium (MS medium, 3 mg/L NAA, 3% sucrose, pH 5.8) with or without 0.8% agar. To the best of our knowledge, this is the first paper reporting plant regeneration from leaf explants in the herbal plant W. chinensis.

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