scholarly journals DNA Fingerprinting and Diversity Analysis of BRRI Hybrid Varieties and their Corresponding Parents

2011 ◽  
Vol 21 (2) ◽  
pp. 189-198
Author(s):  
M.M. Islam ◽  
M.E. Hoque ◽  
S.M.H.A. Rabbi ◽  
M.S. Ali
Keyword(s):  
Microsatellite Markers ◽  
Dna Fingerprinting ◽  
Hybrid Rice ◽  
Similarity Coefficient ◽  
Diversity Analysis ◽  
Allele Number ◽  
Information Contents ◽  
Polymorphic Bands ◽  
Parental Lines ◽  
Genetic Closeness

DNA fingerprinting and genetic diversity of four Bangladesh Rice Research Institute (BRRI) hybrid varieties and their parental lines were carried out. A total of 73 microsatellite markers were tested for screening the genotypes. Among the 73 amplified products, 37% had polymorphic bands showing 81 alleles. The number of alleles per locus ranged from two (RM10) to eight (RM327), where average allele number was 4.333. The Polymorphism Information Contents (PIC) lied between 0.337 (RM10) and 0.852 (RM327). RM327 was the most robust marker providing the highest PIC value (0.852). Pair-wise genetic dissimilarity coefficient interaction showed that BRRI hybrids two was the most genetically distant from each other whereas BRRI hybrids one, three, four and their respective parents were very close. Cluster analysis based on Dice’s similarity coefficient UPGMA system grouped BRRI hybrid and their parental lines into four major clusters at 0.41 cut off similarity coefficient. Four BRRI hybrid varieties grouped into four distinct clusters along with their component lines indicating their genetic closeness. Key words: Hybrid rice, Diversity analysis, Microsatellite markers, DNA fingerprinting   D. O. I. 10.3329/ptcb.v21i2.10242   Plant Tissue Cult. & Biotech. 21(2): 189-198, 2011 (December)

scholarly journals Genetic Diversity Analysis of Hybrid Rice Parental Lines and Genetic Purity Assessment of Hybrid Seeds of China

2020 ◽  
Vol 12 (5) ◽  
pp. 37
Author(s):  
Haiya Cai ◽  
Yuxia Lu ◽  
Gang Liu ◽  
Shuo Zhang ◽  
Haitao Jia ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Hybrid Rice ◽  
Southern China ◽  
Polymorphic Information Content ◽  
Diversity Analysis ◽  
Japonica Rice ◽  
Similarity Coefficients ◽  
Genetic Purity ◽  
Genetic Diversity Analysis ◽  
Parental Lines

Thirty-five pairs of SSR primers were used for genetic diversity analysis and DNA fingerprinting of 31 hybrid rice core parental lines developed in central- and southern-China using one japonica rice line and three inbred rice lines as the check varieties. The average number of alleles (Na) per SSR locus was 4.02, with a range of two to eight, the effective number of alleles (Ne) was 83.16 with a mean of 2.38, ranging from 1.19 to 4.66. The polymorphic information content (PIC) ranged from 0.16 to 0.79, with an average number of 0.52. The results of the cluster analysis indicated that the check varieties viz., one japonica rice and three inbred rice, were clustered into two groups with similarity coefficients of 0.62 and 0.71 respectively indicating their relatedness. Thirty-one hybrid rice parental lines were clustered into 6 groups according to their different types, pedigrees and regions of development with similarity coefficients of approximately 0.76. The highest genetic similarity coefficient (0.94) was observed between Y58S and C815S, and the lowest (0.63) was observed between Quan9311A and Peiai64S. The purity of one hybrid rice cultivar was tested using characteristic marker and the field test, and it was demonstrated that the purities obtained using the two methods were similar. This research will be helpful for rice breeding, new cultivar registration and seed production.

DNA Fingerprinting and Genetic Diversity Analysis of Chilli Germplasm Using Microsatellite Markers

2014 ◽  
Vol 13 (4) ◽  
pp. 174-180 ◽  
Author(s):  
Sumon M. Hossain ◽  
U. Habiba ◽  
Saiful I. Bhuyan ◽  
M.S. Haque ◽  
S.N. Begum ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Dna Fingerprinting ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis

Heritability and Genetic Advance Estimation From Parental Lines of Hybrid Rice

2012 ◽  
Vol 3 (7) ◽  
pp. 11-13
Author(s):  
Arpita Shrivastava ◽  
◽  
D. K. Mishra D. K. Mishra ◽  
G. K. Koutu G. K. Koutu ◽  
S. K. Singh S. K. Singh
Keyword(s):  
Hybrid Rice ◽  
Genetic Advance ◽  
Parental Lines

Development of microsatellite markers at the National Agrobiodiversity Center in Korea for the genetic assessment of underutilized crops

2014 ◽  
Vol 12 (S1) ◽  
pp. S125-S129
Author(s):  
Gi-An Lee ◽  
Sok-Young Lee ◽  
Ho-Sun Lee ◽  
Kyung-Ho Ma ◽  
Jae-Gyun Gwag ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Germplasm Management ◽  
Underutilized Crops ◽  
Crop Species ◽  
Closely Related Species ◽  
Polymorphic Loci ◽  
Allele Number ◽  
The Mean ◽  
Genetic Assessment

The RDA Genebank at the National Agrobiodiversity Center (NAAS, RDA, Republic of Korea) has conserved about 182,000 accessions in 1777 species and is working at preserving agricultural genetic resources for the conservation and sustainable utilization of genetic diversity. The detection of genetic variability in conserved resources is important for germplasm management, but the molecular evaluation tools providing genetic information are insufficient for underutilized crops, unlike those for major crops. In this regard, the Korean National Agrobiodiversity Center has been developing microsatellite markers for several underutilized crops. We designed 3640 primer pairs flanking simple sequence repeat (SSR) motifs for 6310 SSR clones in 21 crop species. Polymorphic loci were revealed in each species (7–36), and the mean ratio of polymorphic loci to all the loci tested was 12%. The average allele number was 5.1 (2.8–10.3) and the expected heterozygosity 0.51 (0.31–0.74). Some SSRs were transferable to closely related species, such as within the genera Fagopyrum and Allium. These SSR markers might be used for studying the genetic diversity of conserved underutilized crops.

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