Fingerprinting of Rice Hybrids and their Parental Lines using Microsatellite Markers and their Utilization in Genetic Purity Assessment of Hybrid Rice

DNA fingerprinting and genetic diversity of four Bangladesh Rice Research Institute (BRRI) hybrid varieties and their parental lines were carried out. A total of 73 microsatellite markers were tested for screening the genotypes. Among the 73 amplified products, 37% had polymorphic bands showing 81 alleles. The number of alleles per locus ranged from two (RM10) to eight (RM327), where average allele number was 4.333. The Polymorphism Information Contents (PIC) lied between 0.337 (RM10) and 0.852 (RM327). RM327 was the most robust marker providing the highest PIC value (0.852). Pair-wise genetic dissimilarity coefficient interaction showed that BRRI hybrids two was the most genetically distant from each other whereas BRRI hybrids one, three, four and their respective parents were very close. Cluster analysis based on Dices similarity coefficient UPGMA system grouped BRRI hybrid and their parental lines into four major clusters at 0.41 cut off similarity coefficient. Four BRRI hybrid varieties grouped into four distinct clusters along with their component lines indicating their genetic closeness. Key words: Hybrid rice, Diversity analysis, Microsatellite markers, DNA fingerprinting D. O. I. 10.3329/ptcb.v21i2.10242 Plant Tissue Cult. & Biotech. 21(2): 189-198, 2011 (December)

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Genetic Diversity Analysis of Hybrid Rice Parental Lines and Genetic Purity Assessment of Hybrid Seeds of China

Journal of Agricultural Science ◽

10.5539/jas.v12n5p37 ◽

2020 ◽

Vol 12 (5) ◽

pp. 37

Author(s):

Haiya Cai ◽

Yuxia Lu ◽

Gang Liu ◽

Shuo Zhang ◽

Haitao Jia ◽

...

Keyword(s):

Genetic Diversity ◽

Hybrid Rice ◽

Southern China ◽

Polymorphic Information Content ◽

Diversity Analysis ◽

Japonica Rice ◽

Similarity Coefficients ◽

Genetic Purity ◽

Genetic Diversity Analysis ◽

Parental Lines

Thirty-five pairs of SSR primers were used for genetic diversity analysis and DNA fingerprinting of 31 hybrid rice core parental lines developed in central- and southern-China using one japonica rice line and three inbred rice lines as the check varieties. The average number of alleles (Na) per SSR locus was 4.02, with a range of two to eight, the effective number of alleles (Ne) was 83.16 with a mean of 2.38, ranging from 1.19 to 4.66. The polymorphic information content (PIC) ranged from 0.16 to 0.79, with an average number of 0.52. The results of the cluster analysis indicated that the check varieties viz., one japonica rice and three inbred rice, were clustered into two groups with similarity coefficients of 0.62 and 0.71 respectively indicating their relatedness. Thirty-one hybrid rice parental lines were clustered into 6 groups according to their different types, pedigrees and regions of development with similarity coefficients of approximately 0.76. The highest genetic similarity coefficient (0.94) was observed between Y58S and C815S, and the lowest (0.63) was observed between Quan9311A and Peiai64S. The purity of one hybrid rice cultivar was tested using characteristic marker and the field test, and it was demonstrated that the purities obtained using the two methods were similar. This research will be helpful for rice breeding, new cultivar registration and seed production.

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EVALUATION OF NEW PROMISING RICE HYBRID AND ITS PARENTAL LINES FOR FLORAL, AGRONOMIC TRAITS AND GENETIC PURITY ASSESSMENT

The Pakistan Journal of Agricultural Sciences ◽

10.21162/pakjas/19.7297 ◽

2019 ◽

Vol 56 (03) ◽

pp. 567-576

Author(s):

Galal Anis

Keyword(s):

Grain Quality ◽

Seed Quality ◽

Hybrid Rice ◽

Agricultural Research ◽

Floral Traits ◽

Research Station ◽

Genetic Purity ◽

Cms Line ◽

Check Variety ◽

Parental Lines

Development of new rice hybrids with high-yielding and superior in grain quality is the most important goal to enhance food security as well as to achieve consumer requirements. High seed costs and poor grain quality are the major limiting factors for adoption of hybrid rice. A practical way to improve hybrid seed quality and to increase production is possible to increase the seed setting rate through improving the floral traits for parental lines. In the context, the study was conducted at the Rice Farm and Laboratories of Sakha Agricultural Research Station, Sakha, Kafr El-Sheikh, Egypt in consecutive three rice growing seasons in 2015, 2016 and 2017. The study focused mainly on characterization of the new promising hybrid ‘G46A/GZ9399R’ and its parental lines. The cytoplasmic male sterile (CMS) line and restorer line were evaluated for twenty-three floral traits that influence outcrossing rate in hybrid rice under two seasons and there was a marked stability for most of these traits. As compared with commercial check variety ‘Giza 178’, the new promising hybrid ‘G46A/GZ9399R’ showed its superiority in all agro-morphological traits except plant height. This hybrid was very early, adapted widely and recorded 20.19% more yield than commercial check variety ‘Giza 178’. Instead, this hybrid produced superiority in all grain quality properties except grain length was medium grain and amylose content was equal for both studied genotypes compared to the check variety. In parallel, the assessment of genetic purity established the SSR markers RM154, RM331, and RM297, amplified alleles specific to differentiate parental lines of hybrid ‘G46A/GZ9399R’.

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