scholarly journals Diferentes estratégias para aumentar a produção de biossurfactante de um isolado de Paenibacillus sp. (BR13834)

2021 ◽  
Vol 10 (17) ◽  
pp. e44101724232
Author(s):  
Elisa Maria de Oliveira ◽  
Victor Hugo Gomes Sales ◽  
Elora Dannan Corrêa Dias ◽  
Wardsson Lustrino Borges ◽  
Marcelo Silva Andrade ◽  
...  
Keyword(s):  

Os biossurfactantes são metabólitos produzidos por diversos microorganismos, e nos últimos anos estão atraindo um interesse na comunidade científica devido suas vantagens em relação aos surfactantes sintéticos. O presente estudo teve como objetivo incrementar a produção de biossurfactante por Paenibacillus sp., otimizando os fatores de crescimento fonte de carbono, pH, temperatura e tempo de cultivo. Inicialmente foram avaliadas as fontes de carbono glicose, lactose, azeite de oliva, óleo de soja, glicerol e querosene. Em seguida, foram avaliados os fatores pH, Temperatura e Tempo, utilizado um delineamento fatorial para a identificação dos fatores que influenciam o processo de produção de biossurfactante. Por fim, os fatores ambientais temperatura e tempo de cultivo foram avaliados usando delineamento central composto rotacional (DCCR). Em todos os ensaios foi utilizado o isolado BR13834 pertencente ao gênero Paenibacillus. O modelo de previsão empírica desenvolvido foi considerado adequado para descrever a produção de biossurfactante em relação à tensão superficial (R2 = 0,755). O valor mínimo para a tensão superficial foi de 34,6 mN/m, obtido nas condições ideais de 30 °C e 24 horas de cultivo. Os resultados demonstraram que o DCCR foi adequado para identificar as melhores condições de produção de biossurfactante produzido por Paenibacillus sp.

Marine Drugs ◽  
2021 ◽  
Vol 19 (4) ◽  
pp. 229
Author(s):  
Young Eun Du ◽  
Eun Seo Bae ◽  
Yeonjung Lim ◽  
Jang-Cheon Cho ◽  
Sang-Jip Nam ◽  
...  

Two new secondary metabolites, svalbamides A (1) and B (2), were isolated from a culture extract of Paenibacillus sp. SVB7 that was isolated from surface sediment from a core (HH17-1085) taken in the Svalbard archipelago in the Arctic Ocean. The combinational analysis of HR-MS and NMR spectroscopic data revealed the structures of 1 and 2 as being lipopeptides bearing 3-amino-2-pyrrolidinone, d-valine, and 3-hydroxy-8-methyldecanoic acid. The absolute configurations of the amino acid residues in svalbamides A and B were determined using the advanced Marfey’s method, in which the hydrolysates of 1 and 2 were derivatized with l- and d- forms of 1-fluoro-2,4-dinitrophenyl-5-alanine amide (FDAA). The absolute configurations of 1 and 2 were completely assigned by deducing the stereochemistry of 3-hydroxy-8-methyldecanoic acid based on DP4 calculations. Svalbamides A and B induced quinone reductase activity in Hepa1c1c7 murine hepatoma cells, indicating that they represent chemotypes with a potential for functioning as chemopreventive agents.


Data in Brief ◽  
2021 ◽  
Vol 35 ◽  
pp. 106784
Author(s):  
Chinda Chhe ◽  
Ayaka Uke ◽  
Sirilak Baramee ◽  
Umbhorn Ungkulpasvich ◽  
Chakrit Tachaapaikoon ◽  
...  

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tongda Li ◽  
Ross Mann ◽  
Jatinder Kaur ◽  
German Spangenberg ◽  
Timothy Sawbridge

AbstractPaenibacillus species are Gram-positive bacteria that have been isolated from a diverse array of plant species and soils, with some species exhibiting plant growth-promoting (PGP) activities. Here we report two strains (S02 and S25) of a novel Paenibacillus sp. that were isolated from perennial ryegrass (Lolium perenne) seeds. Comparative genomics analyses showed this novel species was closely related to P. polymyxa. Genomic analyses revealed that strains S02 and S25 possess PGP genes associated with biological nitrogen fixation, phosphate solubilisation and assimilation, as well as auxin production and transportation. Moreover, secondary metabolite gene cluster analyses identified 13 clusters that are shared by both strains and three clusters unique to S25. In vitro assays demonstrated strong bioprotection activity against phytopathogens (Colletotrichum graminicola and Fusarium verticillioides), particularly for strain S02. A transcriptomics analysis evaluating nitrogen fixation activity showed both strains carry an expressed nif operon, but strain S02 was more active than strain S25 in nitrogen-free media. Another transcriptomics analysis evaluating the interaction of strains with F. verticillioides showed strain S02 had increased expression of core genes of secondary metabolite clusters (fusaricidin, paenilan, tridecaptin and polymyxin) when F. verticillioides was present and absent, compared to S25. Such bioactivities make strain S02 a promising candidate to be developed as a combined biofertiliser/bioprotectant.


2013 ◽  
Vol 66 (8) ◽  
pp. 465-471 ◽  
Author(s):  
Hirohito Kai ◽  
Midori Yamashita ◽  
Shigehiro Takase ◽  
Michizane Hashimoto ◽  
Hideyuki Muramatsu ◽  
...  

2016 ◽  
Vol 9 (3) ◽  
pp. 419-429 ◽  
Author(s):  
Zhihua Yao ◽  
Jiqiang Guo ◽  
Wenzhu Tang ◽  
Zhen Sun ◽  
Yingmin Hou ◽  
...  

PLoS ONE ◽  
2013 ◽  
Vol 8 (4) ◽  
pp. e60538 ◽  
Author(s):  
Arun Nair ◽  
Akika Kuwahara ◽  
Akihiro Nagase ◽  
Haruhiko Yamaguchi ◽  
Tatsuya Yamazaki ◽  
...  

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