scholarly journals Micronutrient deficiency affects the development of the seedlings of the cagaita, a Myrtaceae typical of the Brazilian Cerrado

2020 ◽  
Vol 9 (11) ◽  
pp. e65391110209
Author(s):  
Layara Alexandre Bessa ◽  
Luciana Cristina Vitorino ◽  
Fabiano Guimarães Silva
Keyword(s):  
Growth Parameters ◽  
Nutrient Deficiency ◽  
Micronutrient Deficiency ◽  
Seedling Development ◽  
Brazilian Cerrado ◽  
Development Stages ◽  
Root Hair Development ◽  
Hair Development ◽  
Eugenia Dysenterica ◽  
Cu And Zn

Cagaita (Eugenia dysenterica DC.) plants are highly dependent on the micronutrient iron during their early development stages. Given this, we tested the hypothesis that initial seedling development is affected by a lack of specific micronutrients. We planted seedlings of this species in standard hydroponic solution, and in individual treatments, in which B, Cu, Fe, Mn, Zn or Mo were omitted. The development of symptoms of micronutrient deficiency in response to the different treatment solutions was monitored visually for 120 days after transplantation, and growth parameters and the content of different nutrients in the plant tissue were determined. The deficiency of the micronutrient Mo was the treatment with the greatest effect on seedling growth. The most commonly observed symptoms of nutrient deficiency were chlorosis and necrosis (Cu and Zn deprivation) and impaired root hair development (Fe and Cu deprivation). Overall, Fe was the micronutrient that accumulated most in the stems and roots of the seedlings exposed to the standard nutrient solution, which confirms the importance of the availability of this nutrient in the initial stages of seedling development for the production of healthy cagaita plants, with greater viability.

scholarly journals Macronutrient Omission Affects the Seedling Performance of Eugenia dysenterica DC., an Important Fruiting Species of the Cerrado Biome

2019 ◽  
Vol 11 (11) ◽  
pp. 8 ◽  
Author(s):  
Layara Alexandre Bessa ◽  
Luciana Cristina Vitorino ◽  
Fabianao Guimarães Silva
Keyword(s):  
Seedling Development ◽  
Regional Food ◽  
Cerrado Biome ◽  
Deficiency Symptoms ◽  
Root Hair Development ◽  
Number Of Leaves ◽  
Hair Development ◽  
Eugenia Dysenterica ◽  
Tissue Contents ◽  
Complete Nutrient Solution

Cagaita (Eugenia dysenterica DC.) seedling production is currently important for the restoration of degraded areas of the Cerrado biome and to supply the demand for its fruits for regional food production. The plants of Cagaita have been described in the literature as resistant to nutrient-poor soils, but the studies remain inconclusive. Therefore, we tested the hypothesis that initial E. dysenterica seedling development could be affected by the lack of specific nutrients. We planted seedlings of this species in complete hydroponic solution and under individual macronutrient omission of N, P, K, Ca, Mg and S. The development of macronutrient deficiency symptoms was visually monitored for 120 days after transplantation in response to the treatment solution, and growth characteristics and tissue contents of different macronutrients were determined. The omissions of Mg and P were the treatments with the strongest effects on seedling growth. The most commonly observed deficiency symptoms were chlorosis and necrosis, in addition to leaf purpling (P deprivation), impaired root hair development (Ca and S deprivation) and a reduced number of leaves (Mg and P deprivation). The macronutrients Ca and N were most accumulated in the shoots and roots of plants subjected to complete nutrient solution, showing the importance of the availability of these nutrients in the initial stages of seedling development for the production of healthy Cagaita plants, with greater viability in the field.

scholarly journals 3,4-dehydro-L-proline Induces Programmed Cell Death in the Roots of Brachypodium distachyon

2021 ◽  
Vol 22 (14) ◽  
pp. 7548
Author(s):  
Artur Pinski ◽  
Alexander Betekhtin ◽  
Jolanta Kwasniewska ◽  
Lukasz Chajec ◽  
Elzbieta Wolny ◽  
...  
Keyword(s):  
Cell Death ◽  
Brachypodium Distachyon ◽  
Root Hairs ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Cell Wall Proteins ◽  
Root Epidermis ◽  
Root Hair Development ◽  
Transmission Electron ◽  
Hair Development

As cell wall proteins, the hydroxyproline-rich glycoproteins (HRGPs) take part in plant growth and various developmental processes. To fulfil their functions, HRGPs, extensins (EXTs) in particular, undergo the hydroxylation of proline by the prolyl-4-hydroxylases. The activity of these enzymes can be inhibited with 3,4-dehydro-L-proline (3,4-DHP), which enables its application to reveal the functions of the HRGPs. Thus, to study the involvement of HRGPs in the development of root hairs and roots, we treated seedlings of Brachypodium distachyon with 250 µM, 500 µM, and 750 µM of 3,4-DHP. The histological observations showed that the root epidermis cells and the cortex cells beneath them ruptured. The immunostaining experiments using the JIM20 antibody, which recognizes the EXT epitopes, demonstrated the higher abundance of this epitope in the control compared to the treated samples. The transmission electron microscopy analyses revealed morphological and ultrastructural features that are typical for the vacuolar-type of cell death. Using the TUNEL test (terminal deoxynucleotidyl transferase dUTP nick end labelling), we showed an increase in the number of nuclei with damaged DNA in the roots that had been treated with 3,4-DHP compared to the control. Finally, an analysis of two metacaspases’ gene activity revealed an increase in their expression in the treated roots. Altogether, our results show that inhibiting the prolyl-4-hydroxylases with 3,4-DHP results in a vacuolar-type of cell death in roots, thereby highlighting the important role of HRGPs in root hair development and root growth.

scholarly journals The Histone Chaperone NRP1 Interacts with WEREWOLF to Activate GLABRA2 in Arabidopsis Root Hair Development

2017 ◽  
Vol 29 (2) ◽  
pp. 260-276 ◽  
Author(s):  
Yan Zhu ◽  
Liang Rong ◽  
Qiang Luo ◽  
Baihui Wang ◽  
Nana Zhou ◽  
...  
Keyword(s):  
Root Hair ◽  
Histone Chaperone ◽  
Arabidopsis Root ◽  
Root Hair Development ◽  
Hair Development

scholarly journals A novel gene LbHLH from the halophyte Limonium bicolor enhances salt tolerance via reducing root hair development and enhancing osmotic resistance

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xi Wang ◽  
Yingli Zhou ◽  
Yanyu Xu ◽  
Baoshan Wang ◽  
Fang Yuan
Keyword(s):  
Salt Tolerance ◽  
Root Hair ◽  
Salt Gland ◽  
Nacl Stress ◽  
35S Promoter ◽  
Osmotic Resistance ◽  
Salt Glands ◽  
Limonium Bicolor ◽  
Root Hair Development ◽  
Hair Development

Abstract Background Identifying genes involved in salt tolerance in the recretohalophyte Limonium bicolor could facilitate the breeding of crops with enhanced salt tolerance. Here we cloned the previously uncharacterized gene LbHLH and explored its role in salt tolerance. Results The 2,067-bp open reading frame of LbHLH encodes a 688-amino-acid protein with a typical helix-loop-helix (HLH) domain. In situ hybridization showed that LbHLH is expressed in salt glands of L. bicolor. LbHLH localizes to the nucleus, and LbHLH is highly expressed during salt gland development and in response to NaCl treatment. To further explore its function, we heterologously expressed LbHLH in Arabidopsis thaliana under the 35S promoter. The overexpression lines showed significantly increased trichome number and reduced root hair number. LbHLH might interact with GLABRA1 to influence trichome and root hair development, as revealed by yeast two-hybrid analysis. The transgenic lines showed higher germination percentages and longer roots than the wild type under NaCl treatment. Analysis of seedlings grown on medium containing sorbitol with the same osmotic pressure as 100 mM NaCl demonstrated that overexpressing LbHLH enhanced osmotic resistance. Conclusion These results indicate that LbHLH enhances salt tolerance by reducing root hair development and enhancing osmotic resistance under NaCl stress.

scholarly journals Responses of root hair development to elevated CO2

2011 ◽  
Vol 6 (9) ◽  
pp. 1414-1417 ◽  
Author(s):  
Yao Fang Niu ◽  
Gu Lei Jin ◽  
Ru Shan Chai ◽  
Huan Wang ◽  
Yong Song Zhang
Keyword(s):  
Elevated Co2 ◽  
Root Hair ◽  
Root Hair Development ◽  
Hair Development

scholarly journals Respostas de cultivares de girassol a doses crescentes de boro na entressafra do Cerrado Tocantinense

2016 ◽  
Vol 11 (1) ◽  
pp. 43
Author(s):  
A. Capone ◽  
A. S. Dario ◽  
M. Z. Menegon ◽  
R. R. Fidelis ◽  
H. B. Barros
Keyword(s):  
Boron Concentration ◽  
Block Design ◽  
Micronutrient Deficiency ◽  
Boron Deficiency ◽  
Brazilian Cerrado ◽  
Randomized Block Design ◽  
Sunflower Crop ◽  
Best Responses ◽  
Achene Production ◽  
Leaf Boron

<p class="Default">No cerrado brasileiro a deficiência de boro no solo ocorre com maior frequência. A cultura do girassol é muito sensível à deficiência deste micronutriente, além de apresentar pouca eficiência em seu aproveitamento. Neste contexto, objetivou-se com este estudo avaliar as respostas de cultivares de girassol a doses crescentes de boro no período da entressafra no cerrado tocantinense. O experimento foi conduzido em Gurupi, (TO), com sistema de plantio direto em sucessão a soja, sobre Latossolo Vermelho Amarelo distrófico. O delineamento experimental utilizado foi de blocos casualizados, com quinze tratamentos e quatro repetições. Os tratamentos foram dispostos num esquema fatorial simples 3 x 5, constituído por três cultivares de girassol (variedade Br 122, híbridos Aguará 4 e Helio 863) e cinco doses de ácido bórico (0, 1, 2, 3 e 4 kg ha<sup>-1</sup>). As características avaliadas foram: altura de planta, diâmetro da haste, diâmetro do capítulo, concentração de boro foliar, massa de mil aquênios e produtividade de aquênios. A dose de 3 kg ha<sup>-1</sup> de boro proporcionou, em sua grande maioria, as melhores respostas das cultivares Br 122, Aguará 4 e Helio 863 quanto às características avaliadas. No entanto, com esta dose, a cultivar Aguará 4 apresenta melhor aproveitamento para produção de aquênios.</p><p class="Default" align="center"><strong><em>Responses of sunflower cultivars to increasing doses of boron in the </em></strong><strong><em>between harvests the Cerrado Tocantinense</em></strong><strong><em></em></strong></p><p class="Default"><strong>Abstract</strong><strong>:</strong> In the brazilian cerrado soil boron deficiency occurs more frequently. The sunflower crop is very sensitive to this micronutrient deficiency, and have little efficiency in its use. In that context, aimed to of this study was to evaluate the response of sunflower cultivars to increasing doses of boron during the between harvests in the cerrado tocantinense. The experiment was conducted in Gurupi (TO), with no-tillages soybeans in succession on dystrophic Red Yellow Latosol. The experimental design was a randomized block design with fifteen treatments and four replications. The treatments were arranged in a factorial 3 x 5 simple, composed of three cultivars of sunflower (variety Br 122, hybrid Aguará 4 and Helio 863), and five doses of boric acid (0, 1, 2, 3, and 4 kg ha<sup>-1</sup>). The characteristics evaluated were: plant height, stem diameter, head diameter, leaf boron concentration, weight of thousand achenes and productivity achenes. The dose of 3 kg ha<sup>-1</sup> resulted boron, mostly, the best responses of the cultivars Br 122, Aguará 4 and Helio 863 on the characteristics evaluated. However, at this dose, the cultivar Aguará 4 has better use for achene production.</p><p class="Default"> </p>

Sign in / Sign up

Export Citation Format

Share Document