Aphanomyces astaci genotypes involved in recent crayfish plague outbreaks in central Italy

2018 ◽  
Vol 130 (3) ◽  
pp. 209-219 ◽  
Author(s):  
R Caprioli ◽  
A Mrugała ◽  
M Di Domenico ◽  
V Curini ◽  
C Giansante ◽  
...  
2021 ◽  
Vol 9 ◽  
Author(s):  
Marco Di Domenico ◽  
Valentina Curini ◽  
Riccardo Caprioli ◽  
Carla Giansante ◽  
Agata Mrugała ◽  
...  

The oomycete Aphanomyces astaci is the etiologic agent of crayfish plague, a disease that has seriously impacted the populations of European native crayfish species. The introduction of non-indigenous crayfish of North American origin and their wide distribution across Europe have largely contributed to spread of crayfish plague in areas populated by indigenous crayfish. Tracking A. astaci genotypes may thus be a useful tool for investigating the natural history of crayfish plague in its European range, as well as the sources and introduction pathways of the pathogen. In this study, we describe the development of real-time PCR TaqMan assays aiming to distinguish the five genotype groups of A. astaci (A–E) previously defined by their distinct RAPD patterns. The method was evaluated using DNA extracts from pure A. astaci cultures representing the known genotype groups, and from A. astaci-positive crayfish clinical samples collected mostly during crayfish plague outbreaks that recently occurred in Central Italy and Czechia. The assays do not cross-react with each other, and those targeting genotype groups A, B, D, and E seem sufficiently specific to genotype the pathogen from infected crayfish in the areas invaded by A. astaci (particularly Europe). The unusual A. astaci genotype “SSR-Up” documented from crayfish plague outbreaks in Czechia and chronically infected Pontastacus leptodactylus in the Danube is detected by the group B real-time PCR. The assay originally developed to detect group C (one not yet documented from crayfish plague outbreaks) showed cross-reactivity with Aphanomyces fennicus; the A. astaci genotype “rust1” described in the United States from Faxonius rusticus is detected by that assay as well. Analyses of additional markers (such as sequencing of the nuclear internal transcribed spacer or mitochondrial ribosomal subunits) may complement such cases when the real-time PCR-based genotyping is not conclusive. Despite some limitations, the method is a robust tool for fast genotyping of A. astaci genotype groups common in Europe, both during crayfish plague outbreaks and in latent infections.


Agronomie ◽  
2001 ◽  
Vol 21 (3) ◽  
pp. 267-276
Author(s):  
Daniela Businelli ◽  
Enrico Tombesi ◽  
Marco Trevisan

2019 ◽  
pp. 335-358
Author(s):  
Lorenzo Compagnucci ◽  
Alessio Cavicchi ◽  
Francesca Spigarelli

2015 ◽  
Vol 35 ◽  
pp. 268-271
Author(s):  
Michele Saroli ◽  
Michele Lancia ◽  
Marco Petitta ◽  
Gabriele Scarascia Mugnozza

2018 ◽  
Vol 46 ◽  
pp. 88-93
Author(s):  
Altair Pirro ◽  
Paolo Conti ◽  
Gianluca Cornamusini ◽  
Luigi Carmignani ◽  
Giulia Verdiani ◽  
...  
Keyword(s):  

Author(s):  
Chris Wickham

Amid the disintegration of the Kingdom of Italy in the eleventh and twelfth centuries, a new form of collective government—the commune—arose in the cities of northern and central Italy. This book takes a bold new look at how these autonomous city-states came about, and fundamentally alters our understanding of one of the most important political and cultural innovations of the medieval world. The book provides richly textured portraits of three cities—Milan, Pisa, and Rome—and sets them against a vibrant backcloth of other towns. It argues that, in all but a few cases, the élite of these cities and towns developed one of the first nonmonarchical forms of government in medieval Europe, unaware that they were creating something altogether new. The book makes clear that the Italian city commune was by no means a democracy in the modern sense, but that it was so novel that outsiders did not know what to make of it. It describes how, as the old order unraveled, the communes emerged, governed by consular elites “chosen by the people,” and subject to neither emperor nor king. They regularly fought each other, yet they grew organized and confident enough to ally together to defeat Frederick Barbarossa, the German emperor, at the Battle of Legnano in 1176. This book reveals how the development of the autonomous city-state took place, which would in the end make possible the robust civic culture of the Renaissance.


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