scholarly journals A Twin DNA Replication Factory

2020 ◽  
Author(s):  
Michael O’Donnell
Keyword(s):  
Dna Replication ◽  
Replication Factory

scholarly journals Anatomy of a twin DNA replication factory

2020 ◽  
Vol 48 (6) ◽  
pp. 2769-2778
Author(s):  
Huilin Li ◽  
Nina Y. Yao ◽  
Michael E. O'Donnell
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Bacillus Subtilis ◽  
Dna Replication ◽  
In Vivo Studies ◽  
Replication Forks ◽  
Structural Studies ◽  
Replication Factory ◽  
Higher Eukaryotes

The replication of DNA in chromosomes is initiated at sequences called origins at which two replisome machines are assembled at replication forks that move in opposite directions. Interestingly, in vivo studies observe that the two replication forks remain fastened together, often referred to as a replication factory. Replication factories containing two replisomes are well documented in cellular studies of bacteria (Escherichia coli and Bacillus subtilis) and the eukaryote, Saccharomyces cerevisiae. This basic twin replisome factory architecture may also be preserved in higher eukaryotes. Despite many years of documenting the existence of replication factories, the molecular details of how the two replisome machines are tethered together has been completely unknown in any organism. Recent structural studies shed new light on the architecture of a eukaryote replisome factory, which brings with it a new twist on how a replication factory may function.

scholarly journals ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin

2012 ◽  
Vol 200 (1) ◽  
pp. 31-44 ◽  
Author(s):  
Kyoo-young Lee ◽  
Haiqing Fu ◽  
Mirit I. Aladjem ◽  
Kyungjae Myung
Keyword(s):  
Dna Replication ◽  
Molecular Mechanisms ◽  
S Phase ◽  
Nuclear Antigen ◽  
Spatial Regulation ◽  
Replication Factory ◽  
G2 Phase ◽  
Phase Progression ◽  
Temporal And Spatial ◽  
Proliferating Cell

Temporal and spatial regulation of the replication factory is important for efficient DNA replication. However, the underlying molecular mechanisms are not well understood. Here, we report that ATAD5 regulates the lifespan of replication factories. Reduced expression of ATAD5 extended the lifespan of replication factories by retaining proliferating cell nuclear antigen (PCNA) and other replisome proteins on the chromatin during and even after DNA synthesis. This led to an increase of inactive replication factories with an accumulation of replisome proteins. Consequently, the overall replication rate was decreased, which resulted in the delay of S-phase progression. Prevalent detection of PCNA foci in G2 phase cells after ATAD5 depletion suggests that defects in the disassembly of replication factories persist after S phase is complete. ATAD5-mediated regulation of the replication factory and PCNA required an intact ATAD5 ATPase domain. Taken together, our data imply that ATAD5 regulates the cycle of DNA replication factories, probably through its PCNA-unloading activity.

scholarly journals A moving DNA replication factory in Caulobacter crescentus

2001 ◽  
Vol 20 (17) ◽  
pp. 4952-4963 ◽  
Author(s):  
R. B. Jensen
Keyword(s):  
Dna Replication ◽  
Caulobacter Crescentus ◽  
Replication Factory

Systematic localisation of proteins fused to the green fluorescent protein inBacillus subtilis: Identification of new proteins at the DNA replication factory

PROTEOMICS ◽  
2006 ◽  
Vol 6 (7) ◽  
pp. 2135-2146 ◽  
Author(s):  
Jean-Christophe Meile ◽  
Ling Juan Wu ◽  
S. Dusko Ehrlich ◽  
Jeff Errington ◽  
Philippe Noirot
Keyword(s):  
Green Fluorescent Protein ◽  
Dna Replication ◽  
Fluorescent Protein ◽  
Replication Factory ◽  
Green Fluorescent

Replication of Bacteriophage 186 DNA

1972 ◽  
Vol 30 ◽  
pp. 194-195
Author(s):  
Dhruba K. Chattoraj ◽  
Ross B. Inman
Keyword(s):  
Electron Microscopy ◽  
Dna Replication ◽  
Frame Of Reference ◽  
Dna Molecules ◽  
E Coli ◽  
Phage Dna ◽  
Partial Denaturation

Electron microscopy of replicating intermediates has been quite useful in understanding the mechanism of DNA replication in DNA molecules of bacteriophage, mitochondria and plasmids. The use of partial denaturation mapping has made the tool more powerful by providing a frame of reference by which the position of the replicating forks in bacteriophage DNA can be determined on the circular replicating molecules. This provided an easy means to find the origin and direction of replication in λ and P2 phage DNA molecules. DNA of temperate E. coli phage 186 was found to have an unique denaturation map and encouraged us to look into its mode of replication.

Molecular Themes in DNA Replication

2009 ◽  
Keyword(s):  
Dna Replication

The infection and viral DNA replication ofBocavirusminute virus of canine in permissive cells and non-permissive cells

2010 ◽  
Vol 34 (8) ◽  
pp. S60-S60
Author(s):  
Yuning Sun ◽  
Fang Li ◽  
Jianming Qiu ◽  
Xiaohong Lu
Keyword(s):  
Dna Replication ◽  
Viral Dna ◽  
Viral Dna Replication
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