scholarly journals FLUORESCENCE CHARACTERISTICS OF RHODAMINE 6G AND RHODAMINE C IN WATER-MICELLAR SURFACTANT ENVIRONMENTS

2019 ◽  
Vol 85 (12) ◽  
pp. 84-95
Author(s):  
Olga Zaporozhets ◽  
Sergey Kulichenko ◽  
Sergey Lelyushok ◽  
Viktoriia Klovak
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Aqueous Solutions ◽  
Fluorescence Intensity ◽  
Cationic Surfactants ◽  
Rhodamine 6G ◽  
Sodium Dodecyl ◽  
Hydrocarbon Radical ◽  
Fluorescence Characteristics ◽  
Dodecyl Sulfate ◽  
Triton X

The influence of cationic, anionic, nonionic surfactants and their mixtures on the fluorescence characteristics of rhodamine 6G and Rho-damine C solutions has been investigated. The fluorescence intensity of aqueous solutions of rhodamine 6G and in the presence of cetylpyridinium chloride and sodium dodecyl sulfate has almost unchanged throughout the pH range. The fluorescence intensity of aqueous and water-micellar rhodamine C solutions has been increased in the pH 1-4 range; the signal has been remained unchanged at high pH values. The studies have been carried out at pH 4 for rhodamine 6G and at pH 10 for rhodamine C. The fluorescence characteristics of water-micellar dye - surfactant - non-ionic surfactant systems have been performed at a concentration of Triton X-100 of 3.4·10‑2 mol/l. The interaction with cationic surfactants has shown differences character between the I=f(n) dependences for aqueous solutions of highly hydrophobic rhodamine 6G and more hydrophilic rhodamine C. The study of the effect of the hydrocarbon radical length on the intensity of the fluorescence of rhodamine 6G and rhodamine C has been carried out at two concentrations of cationic surfactants: under the condition of the formation of stoichiometric associates dye with cationic surfactant and in the region of the micellar concentrations of cationic surfactants. The character of the influence of the length of the hydrocarbon radical cationic surfactants on the fluorescence intensity of the dyes can be explained by the increasing role of hydrophobic interactions and the enhancement of solubilization in systems involving long-chain surfactants. The difference in the nature of the associates of rhodamine 6G and rhodamine C with hydrophobic and moderately hydrophobic cationic surfactants has been counterbalanced in the presence of Triton X-100. Reduction of fluorescence intensity of rhodamine 6G in domicile solutions of anionic sodium dodecyl sulfate has been established. The method of fluorescence detection of sodium dodecyl sulfate in reaction with rhodamine 6G has been proposed. The method has been tested in determining of anionic surfactants in the waters after washing clothes.

Solubility and adsorption behaviors of chlorophenols in the presence of surfactant

1997 ◽  
Vol 35 (7) ◽  
pp. 123-130 ◽  
Author(s):  
J. C. Liu ◽  
P. S. Chang
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Critical Micelle Concentration ◽  
Sodium Dodecyl ◽  
Adsorption Behaviors ◽  
Brij 35 ◽  
Important Index ◽  
Dodecyl Sulfate ◽  
Triton X

The solubility of chlorophenols as affected by surfactant was investigated. Three kinds of surfactant, sodium dodecyl sulfate, Triton X-100, and Brij 35, were utilized. The solubilization of chlorophenols by surfactant follows the order of 2,4,6-trichlorophenol > 2,4-dichlorophenol > 2,6-dichlorophenol > 2-chlorophenol; and the critical micelle concentration is an important index. The adsorption reactions of 2,4-dichlorophenol and 2,4,6- trichlorophenol onto hydrous montmorillonite in the presence of surfactant were examined. The presence of surfactant decreased the adsorption of chlorophenols significantly. The roles of hydrophobicity of chlorophenols in solubilization and adsorption behaviors are discussed.

Different submicellar solubilization mechanisms revealed by 1H NMR and 2D diffusion ordered spectroscopy (DOSY)

2020 ◽  
Vol 22 (19) ◽  
pp. 11075-11085
Author(s):  
Mengjian Wu ◽  
Zhaoxia Wu ◽  
Shangwu Ding ◽  
Zhong Chen ◽  
Xiaohong Cui
Keyword(s):  
Nmr Spectroscopy ◽  
Sodium Dodecyl Sulfate ◽  
1H Nmr ◽  
Sodium Dodecyl ◽  
Butyl Methacrylate ◽  
H Nmr ◽  
Molecular Scale ◽  
Two Systems ◽  
Dodecyl Sulfate ◽  
Triton X

Different submicellar solubilization mechanisms of two systems, Triton X-100/tetradecane and sodium dodecyl sulfate (SDS)/butyl methacrylate, are revealed on the molecular scale by 1H NMR spectroscopy and 2D diffusion ordered spectroscopy (DOSY).

Extraction and properties of hemagglutinin from cell wall fragments of Fusobacterium nucleatum

1982 ◽  
Vol 152 (1) ◽  
pp. 298-305
Author(s):  
P Dehazya ◽  
R S Coles
Keyword(s):  
Cell Wall ◽  
Sodium Dodecyl Sulfate ◽  
Specific Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Fusobacterium Nucleatum ◽  
Blue Staining ◽  
Sephadex Column ◽  
Dodecyl Sulfate ◽  
Triton X

To study the hemagglutinin of Fusobacterium nucleatum, methods were sought to solubilize and purify this component. When cells of F. nucleatum were ruptured by passage through a French press, the fragments lost virtually all ability to agglutinate human erythrocytes. Extraction of the fragments with 2% Triton X-100 for 30 min at 22 degrees C restored hemagglutinating activity (HA). Hemagglutination by these fragments could be inhibited by arginine, as can hemagglutination by intact bacteria. Treatment of active cell wall fragments with pronase and 2% Triton X-100-EDTA at 37 degrees C or with pronase and 0.1% Triton X-100-EDTA at pH 10.0 allowed recovery of solubilized HA. The former HA was inhibited by arginine (arg+) whereas the latter was not (arg-). Fractionation of the arg+ extract by preparative isoelectric focusing showed that HA was recovered from the gel sections having a pH between 4.5 and 5.5. Hemagglutination by this preparation was still arg+. Chromatography of this hemagglutinin on DEAE-Sephadex increased the specific activity to high levels with a loss of inhibition by arginine. A fraction from the DEAE-Sephadex column containing 10,700 HA units per mg of protein was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Solubilization at 22 degrees C before electrophoresis revealed three Coomassie blue-staining bands which migrated with apparent molecular weights of about 21,000, 38,000 and 60,000. When the same DEAE fraction was boiled in sodium dodecyl sulfate, electrophoresis revealed only one band with an apparent molecular weight of 21,000.

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