Porcine carotid arteries decellularized with a suitable concentration combination of Triton X-100 and sodium dodecyl sulfate for tissue engineering vascular grafts

2020 ◽  
Author(s):  
Zhiwen Cai ◽  
Yongquan Gu ◽  
Yonghao Xiao ◽  
Cong Wang ◽  
Zhonggao Wang
Keyword(s):  
Tissue Engineering ◽  
Sodium Dodecyl Sulfate ◽  
Carotid Arteries ◽  
Vascular Grafts ◽  
Sodium Dodecyl ◽  
Dodecyl Sulfate ◽  
Triton X

Decellularization of porcine carotid arteries using low-concentration sodium dodecyl sulfate

2020 ◽  
pp. 039139882097542
Author(s):  
Jin Cheng ◽  
Ji Li ◽  
Zhiwen Cai ◽  
Yuehao Xing ◽  
Cong Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Sodium Dodecyl Sulfate ◽  
Carotid Arteries ◽  
Sodium Dodecyl ◽  
Dimensional Structure ◽  
Low Concentration ◽  
Dodecyl Sulfate ◽  
Decellularized Scaffolds ◽  
Triton X

Background: The decellularized scaffold is a promising material for producing tissue-engineered vascular grafts (TEVGs) because of its complex, native-like three-dimensional structure and mechanical properties. Sodium dodecyl sulfate (SDS), one of the most commonly used decellularization reagents, appears to be more effective than other detergents for removing cells from dense tissues. The concentrations of SDS used in previous studies and their effects on decellularization are not consistent. Methods: In this study, porcine carotid arteries were decellularized using detergent-based protocols using Triton X-100 followed by SDS at different concentrations and exposing time. Cell removal efficiency and composition were evaluated by histological analysis, and DNA and collagen quantification. Ultrastructure, mechanical properties, pore size distribution, and in vivo biocompatibility of decellularized arteries were also evaluated. Results: The DNA content of decellularized scaffolds treated with 0.3% SDS for 72 h or 0.5% SDS for 48 h was significantly less than that treated with 1% SDS for 30 h. There was a significant loss of soluble collagen after treatment with 1% SDS relative to native arteries. The extensive loss of elastin and glycosaminoglycans was observed in decellularized arteries treated with 0.5% SDS or 1% SDS. The basement membrane and biomechanics were also damaged by these two protocols. Moreover, decellularized scaffolds became more porous with many large pores after treatment with 0.3% SDS. Conclusion: Low-concentration SDS could be a suitable choice for artery decellularization. Decellularized porcine carotid arteries, prepared using Triton X-100 followed by 0.3% SDS, may be a promising biological scaffold for TEVGs.

Solubility and adsorption behaviors of chlorophenols in the presence of surfactant

1997 ◽  
Vol 35 (7) ◽  
pp. 123-130 ◽  
Author(s):  
J. C. Liu ◽  
P. S. Chang
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Critical Micelle Concentration ◽  
Sodium Dodecyl ◽  
Adsorption Behaviors ◽  
Brij 35 ◽  
Important Index ◽  
Dodecyl Sulfate ◽  
Triton X

The solubility of chlorophenols as affected by surfactant was investigated. Three kinds of surfactant, sodium dodecyl sulfate, Triton X-100, and Brij 35, were utilized. The solubilization of chlorophenols by surfactant follows the order of 2,4,6-trichlorophenol > 2,4-dichlorophenol > 2,6-dichlorophenol > 2-chlorophenol; and the critical micelle concentration is an important index. The adsorption reactions of 2,4-dichlorophenol and 2,4,6- trichlorophenol onto hydrous montmorillonite in the presence of surfactant were examined. The presence of surfactant decreased the adsorption of chlorophenols significantly. The roles of hydrophobicity of chlorophenols in solubilization and adsorption behaviors are discussed.

Different submicellar solubilization mechanisms revealed by 1H NMR and 2D diffusion ordered spectroscopy (DOSY)

2020 ◽  
Vol 22 (19) ◽  
pp. 11075-11085
Author(s):  
Mengjian Wu ◽  
Zhaoxia Wu ◽  
Shangwu Ding ◽  
Zhong Chen ◽  
Xiaohong Cui
Keyword(s):  
Nmr Spectroscopy ◽  
Sodium Dodecyl Sulfate ◽  
1H Nmr ◽  
Sodium Dodecyl ◽  
Butyl Methacrylate ◽  
H Nmr ◽  
Molecular Scale ◽  
Two Systems ◽  
Dodecyl Sulfate ◽  
Triton X

Different submicellar solubilization mechanisms of two systems, Triton X-100/tetradecane and sodium dodecyl sulfate (SDS)/butyl methacrylate, are revealed on the molecular scale by 1H NMR spectroscopy and 2D diffusion ordered spectroscopy (DOSY).

Extraction and properties of hemagglutinin from cell wall fragments of Fusobacterium nucleatum

1982 ◽  
Vol 152 (1) ◽  
pp. 298-305
Author(s):  
P Dehazya ◽  
R S Coles
Keyword(s):  
Cell Wall ◽  
Sodium Dodecyl Sulfate ◽  
Specific Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Fusobacterium Nucleatum ◽  
Blue Staining ◽  
Sephadex Column ◽  
Dodecyl Sulfate ◽  
Triton X

To study the hemagglutinin of Fusobacterium nucleatum, methods were sought to solubilize and purify this component. When cells of F. nucleatum were ruptured by passage through a French press, the fragments lost virtually all ability to agglutinate human erythrocytes. Extraction of the fragments with 2% Triton X-100 for 30 min at 22 degrees C restored hemagglutinating activity (HA). Hemagglutination by these fragments could be inhibited by arginine, as can hemagglutination by intact bacteria. Treatment of active cell wall fragments with pronase and 2% Triton X-100-EDTA at 37 degrees C or with pronase and 0.1% Triton X-100-EDTA at pH 10.0 allowed recovery of solubilized HA. The former HA was inhibited by arginine (arg+) whereas the latter was not (arg-). Fractionation of the arg+ extract by preparative isoelectric focusing showed that HA was recovered from the gel sections having a pH between 4.5 and 5.5. Hemagglutination by this preparation was still arg+. Chromatography of this hemagglutinin on DEAE-Sephadex increased the specific activity to high levels with a loss of inhibition by arginine. A fraction from the DEAE-Sephadex column containing 10,700 HA units per mg of protein was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Solubilization at 22 degrees C before electrophoresis revealed three Coomassie blue-staining bands which migrated with apparent molecular weights of about 21,000, 38,000 and 60,000. When the same DEAE fraction was boiled in sodium dodecyl sulfate, electrophoresis revealed only one band with an apparent molecular weight of 21,000.

scholarly journals ISOLATION AND REACTIVATION OF THE AXOSTYLE

1973 ◽  
Vol 56 (1) ◽  
pp. 13-26 ◽  
Author(s):  
Mark S. Mooseker ◽  
Lewis G. Tilney
Keyword(s):  
Molecular Weight ◽  
Sodium Dodecyl Sulfate ◽  
Atpase Activity ◽  
Adenosine Triphosphate ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gels ◽  
Molecular Weights ◽  
Dodecyl Sulfate ◽  
Cilia And Flagella ◽  
Triton X

The contractile axostyle is a ribbon-shaped organelle present in certain species of flagellates found in the hindgut of wood eating insects. This organelle propagates an undulatory wave whose motion, like flagella and cilia, is related to microtubules. Unlike the axoneme of cilia and flagella, however, the axostyle is composed of singlet microtubules linked together in parallel rows. Axostyles were isolated from Cryptocercus gut protozoa with Triton X-100. Normal motility of the isolated axostyle could be restored with adenosine triphosphate (ATP); the specific conditions necessary for this reactivation were essentially identical with those reported for the reactivation of isolated flagella or whole sperm. ATPase activity of the isolated axostyle was comparable to the values reported for ciliary or flagellar axonemes. The axostyle was reasonably specific for ATP. Most of the proteins of the isolated axostyle comigrated with proteins of the ciliary axoneme on sodium dodecyl sulfate (SDS) polyacrylamide gels (i e. equivalent molecular weights). These included the following: the higher molecular weight component of dynein, tubulin, linkage protein (nexin), and various secondary proteins. Evidence for dynein in the axostyle is presented and a model proposed to explain how repeated propagated waves can be generated.

scholarly journals Decellularization of Kidney Tissue: Comparison of Sodium Lauryl Ether Sulfate and Sodium Dodecyl Sulfate for Allotransplantation in Rat

2020 ◽  
Author(s):  
Mohammad Amin Keshvari ◽  
Alireza Afshar ◽  
Sajad Daneshi ◽  
Arezoo Khoradmehr ◽  
Mandana Baghban ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Kidney Tissue ◽  
Dna Quantification ◽  
Sodium Lauryl Ether Sulfate ◽  
Ether Sulfate ◽  
Lauryl Ether ◽  
Dodecyl Sulfate ◽  
Decellularized Scaffolds

Chronic kidney diseases (CKD) and end stage renal disease (ESRD) are growing threats worldwide. Tissue engineering is a new hope to surpass the current limitations such as the shortage of donor. To do so, the first step would be fabrication of an intact decellularized kidney scaffold. In the current study, an automatic decellularization device was developed to perfuse and decellularize male rats' kidneys using both sodium lauryl ether sulfate (SLES) and sodium dodecyl sulfate (SDS) and to compare their efficacy in kidney decellularization and post-transplantation angiogenesis. After anesthesia, kidneys were perfused with either 1% SDS solution for 4 h or 1% SLES solution for 6 h. The decellularized scaffolds were stained with hematoxylin and eosin (H&E), periodic acid Schiff (PAS), Masson’s trichrome, and alcian blue to determine cell removal and glycogen, collagen and glycosaminoglycans (GAGs) contents, respectively. Moreover, scanning electron microscopy (SEM) was performed to evaluate the cell removal and preservation of microarchitecture of both SDS and SLES scaffolds. Additionally, DNA quantification assay was applied for all groups in order to measure residual DNA in the scaffolds and normal kidney. In order to demonstrate biocompatibility and bioactivity of the decellularized scaffolds, allotransplantation was performed in back muscle and angiogenesis was evaluated. Complete cell removal in both SLES and SDS groups was observed in SEM and DNA quantification assays. Moreover, the extracellular matrix (ECM) architecture of rat kidney in the SLES group was significantly preservation better than the SDS group was shown. The formation of blood capillaries and vessels were observed in the kidney allotransplantations in both SLES and SDS decellularized kidneys. In conclusion, we demonstrated that both SLES and SDS could be promising tools in kidney tissue engineering. The better preservation of ECM than SDS, introduces SLES as the solvent of choice for kidney decellularization. ¬¬

scholarly journals FLUORESCENCE CHARACTERISTICS OF RHODAMINE 6G AND RHODAMINE C IN WATER-MICELLAR SURFACTANT ENVIRONMENTS

2019 ◽  
Vol 85 (12) ◽  
pp. 84-95
Author(s):  
Olga Zaporozhets ◽  
Sergey Kulichenko ◽  
Sergey Lelyushok ◽  
Viktoriia Klovak
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Aqueous Solutions ◽  
Fluorescence Intensity ◽  
Cationic Surfactants ◽  
Rhodamine 6G ◽  
Sodium Dodecyl ◽  
Hydrocarbon Radical ◽  
Fluorescence Characteristics ◽  
Dodecyl Sulfate ◽  
Triton X

The influence of cationic, anionic, nonionic surfactants and their mixtures on the fluorescence characteristics of rhodamine 6G and Rho-damine C solutions has been investigated. The fluorescence intensity of aqueous solutions of rhodamine 6G and in the presence of cetylpyridinium chloride and sodium dodecyl sulfate has almost unchanged throughout the pH range. The fluorescence intensity of aqueous and water-micellar rhodamine C solutions has been increased in the pH 1-4 range; the signal has been remained unchanged at high pH values. The studies have been carried out at pH 4 for rhodamine 6G and at pH 10 for rhodamine C. The fluorescence characteristics of water-micellar dye - surfactant - non-ionic surfactant systems have been performed at a concentration of Triton X-100 of 3.4·10‑2 mol/l. The interaction with cationic surfactants has shown differences character between the I=f(n) dependences for aqueous solutions of highly hydrophobic rhodamine 6G and more hydrophilic rhodamine C. The study of the effect of the hydrocarbon radical length on the intensity of the fluorescence of rhodamine 6G and rhodamine C has been carried out at two concentrations of cationic surfactants: under the condition of the formation of stoichiometric associates dye with cationic surfactant and in the region of the micellar concentrations of cationic surfactants. The character of the influence of the length of the hydrocarbon radical cationic surfactants on the fluorescence intensity of the dyes can be explained by the increasing role of hydrophobic interactions and the enhancement of solubilization in systems involving long-chain surfactants. The difference in the nature of the associates of rhodamine 6G and rhodamine C with hydrophobic and moderately hydrophobic cationic surfactants has been counterbalanced in the presence of Triton X-100. Reduction of fluorescence intensity of rhodamine 6G in domicile solutions of anionic sodium dodecyl sulfate has been established. The method of fluorescence detection of sodium dodecyl sulfate in reaction with rhodamine 6G has been proposed. The method has been tested in determining of anionic surfactants in the waters after washing clothes.

Sign in / Sign up

Export Citation Format

Share Document