Young albino rats were fed choline-deficient and choline-supplemented diets, fat-free and containing two different levels of fat (4%, 30%), for 2 weeks. The fatty acid pattern of the major lipid fractions of liver, serum, and adipose tissue was determined by thin-layer and gas–liquid chromatography. It was found that (a) no quantitative similarities in fatty acid pattern existed between hepatic triglycerides on the one hand and adipose tissue, serum triglycerides, and dietary fat on the other, but the fatty acid composition of hepatic triglycerides bore a closer resemblance to that of dietary fat when the latter was increased to 30%; (b) under the conditions of this experiment, the level of dietary fat had a more marked effect on altering the fatty acid composition of tissues, especially the triglyceride fraction, than the level of choline in the diet; and (c) a relative preponderance of 16-carbon fatty acids occurred in hepatic triglycerides of choline-deficient animals fed the fat-free and low-fat diets.
Fatty Acid Composition of Egg Yolk and Adipose Tissue as Influenced by Dietary Fat and Strain of Hen
