Betaine improves the growth performance and muscle growth of partridge shank broiler chickens via altering myogenic gene expression and insulin-like growth factor-1 signaling pathway

We have investigated the hypothesis that there is local regulation of insulin-like growth factor (IGF) gene expression during skeletal muscle growth. Compensatory hypertrophy was induced in the soleus, a predominantly slow-twitch muscle, and plantaris, a fast-twitch muscle, in 11- to 12-wk-old female Wistar rats by unilateral cutting of the distal gastrocnemius tendon. Animals were killed 2, 4, or 8 days later, and muscles of the nonoperated leg served as controls. Muscle weight increased throughout the experimental period, reaching 127% (soleus) or 122% (plantaris) of control values by day 8. In both growing muscles, IGF-I mRNA, quantitated by a solution-hybridization nuclease-protection assay, rose by nearly threefold on day 2 and remained elevated throughout the experimental period. IGF-II mRNA levels also increased over controls. A more dramatic response was seen in hypophysectomized rats, where IGF-I mRNA levels rose by 8- to 13-fold, IGF-II values by 3- to 7-fold, and muscle mass increased on day 8 to 149% (soleus) or 133% (plantaris) of the control contralateral limb. These results indicate that signals propagated during muscle hypertrophy enhance the expression of both IGF genes, that modulation of IGF-I mRNA levels can occur in the absence of growth hormone, and that locally produced IGF-I and IGF-II may play a role in skeletal muscle growth.

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Influence of Lacto-Immuno-Vital on growth performance and gene expression of IgA, MUC-2, and growth factor IGF-2 in the jejunum of broiler chickens

Poultry Science ◽

10.1016/j.psj.2020.09.054 ◽

2020 ◽

Vol 99 (12) ◽

pp. 6569-6575

Author(s):

M. Levkut ◽

V. Karaffová ◽

M. Levkutová ◽

V. Seman ◽

V. Revajová ◽

...

Keyword(s):

Gene Expression ◽

Growth Factor ◽

Growth Performance ◽

Broiler Chickens

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New Role for Serum Response Factor in Postnatal Skeletal Muscle Growth and Regeneration via the Interleukin 4 and Insulin-Like Growth Factor 1 Pathways

Molecular and Cellular Biology ◽

10.1128/mcb.00138-06 ◽

2006 ◽

Vol 26 (17) ◽

pp. 6664-6674 ◽

Cited By ~ 53

Author(s):

Claude Charvet ◽

Christophe Houbron ◽

Ara Parlakian ◽

Julien Giordani ◽

Charlotte Lahoute ◽

...

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

Growth Factor ◽

Serum Response Factor ◽

Muscle Growth ◽

Interleukin 4 ◽

Specific Gene ◽

Response Factor ◽

Insulin Like Growth Factor ◽

Serum Response

ABSTRACT Serum response factor (SRF) is a crucial transcriptional factor for muscle-specific gene expression. We investigated SRF function in adult skeletal muscles, using mice with a postmitotic myofiber-targeted disruption of the SRF gene. Mutant mice displayed severe skeletal muscle mass reductions due to a postnatal muscle growth defect resulting in highly hypotrophic adult myofibers. SRF-depleted myofibers also failed to regenerate following injury. Muscles lacking SRF had very low levels of muscle creatine kinase and skeletal alpha-actin (SKA) transcripts and displayed other alterations to the gene expression program, indicating an overall immaturity of mutant muscles. This loss of SKA expression, together with a decrease in beta-tropomyosin expression, contributed to myofiber growth defects, as suggested by the extensive sarcomere disorganization found in mutant muscles. However, we observed a downregulation of interleukin 4 (IL-4) and insulin-like growth factor 1 (IGF-1) expression in mutant myofibers which could also account for their defective growth and regeneration. Indeed, our demonstration of SRF binding to interleukin 4 and IGF-1 promoters in vivo suggests a new crucial role for SRF in pathways involved in muscle growth and regeneration.

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Sorbic acid improves growth performance and regulates insulin-like growth factor system gene expression in swine1

Journal of Animal Science ◽

10.2527/jas.2010-3677 ◽

2011 ◽

Vol 89 (8) ◽

pp. 2356-2364 ◽

Cited By ~ 10

Author(s):

Z.-F. Luo ◽

X.-L. Fang ◽

G. Shu ◽

S.-B. Wang ◽

X.-T. Zhu ◽

...

Keyword(s):

Gene Expression ◽

Growth Factor ◽

Growth Performance ◽

Sorbic Acid ◽

Insulin Like Growth Factor ◽

Factor System

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Identification of the possible therapeutic targets in the insulin-like growth factor 1 receptor pathway in a cohort of Egyptian hepatocellular carcinoma complicating chronic hepatitis C type 4

Drug Target Insights ◽

10.33393/dti.2020.1548 ◽

2020 ◽

Vol 14 (1) ◽

pp. 1-11

Author(s):

Nada M.K. Mabrouk ◽

Dalal M. Elkaffash ◽

Mona Abdel-Hadi ◽

Salah-ElDin Abdelmoneim ◽

Sameh Saad ElDeen ◽

...

Keyword(s):

Gene Expression ◽

Hepatocellular Carcinoma ◽

Hepatitis C ◽

Growth Factor ◽

Signaling Pathway ◽

Pathway Analysis ◽

Expression Patterns ◽

Gene Expression Patterns ◽

Insulin Like Growth Factor ◽

Tumor Characteristics

Background: Molecular targeted drugs are the first line of treatment of advanced hepatocellular carcinoma (HCC) due to its chemo- and radioresistant nature. HCC has several well-documented etiologic factors that drive hepatocarcinogenesis through different molecular pathways. Currently, hepatitis C virus (HCV) is a leading cause of HCC. Therefore, we included a unified cohort of HCV genotype 4-related HCCs to study the expression levels of genes involved in the insulin-like growth factor 1 receptor (IGF1R) pathway, which is known to be involved in all aspects of cancer growth and progression. Aim: Determine the gene expression patterns of IGF1R pathway genes in a cohort of Egyptian HCV-related HCCs. Correlate them with different patient/tumor characteristics. Determine the activity status of involved pathways. Methods: Total ribonucleic acid (RNA) was extracted from 32 formalin-fixed paraffin-embedded tissues of human HCV-related HCCs and 6 healthy liver donors as controls. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) using RT2 Profiler PCR Array for Human Insulin Signaling Pathway was done to determine significantly up- and downregulated genes with identification of most frequently coregulated genes, followed by correlation of gene expression with different patient/tumor characteristics. Finally, canonical pathway analysis was performed using the Ingenuity Pathway Analysis software. Results: Six genes – AEBP1, AKT2, C-FOS, PIK3R1, PRKCI, SHC1 – were significantly overexpressed. Thirteen genes – ADRB3, CEBPA, DUSP14, ERCC1, FRS3, IGF2, INS, IRS1, JUN, MTOR, PIK3R2, PPP1CA, RPS6KA1 – were significantly underexpressed. Several differentially expressed genes were related to different tumor/patient characteristics. Nitric oxide and reactive oxygen species production pathway was significantly activated in the present cohort, while the growth hormone signaling pathway was inactive. Conclusions: The gene expression patterns identified in this study may serve as possible therapeutic targets in HCV-related HCCs. The most frequently coregulated genes may serve to guide combined molecular targeted therapies. The IGF1R pathway showed evidence of inactivity in the present cohort of HCV-related HCCs, so targeting this pathway in therapy may not be effective.

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