Immunomodulatory effects of amniotic membrane matrix incorporated into collagen scaffolds

ABSTRACTDefects in craniofacial bones occur congenitally, after high-energy impacts, and during the course of treatment for stroke and cancer. These injuries are difficult to heal due to the overwhelming size of the injury area and the inflammatory environment surrounding the injury. Significant inflammatory response after injury may greatly inhibit regenerative healing. We have developed mineralized collagen scaffolds that can induce osteogenic differentiation and matrix biosynthesis in the absence of osteogenic media or supplemental proteins. The amniotic membrane is derived from placentas and has been recently investigated as an extracellular matrix to prevent chronic inflammation. Herein, we hypothesized that a mineralized collagen-amnion composite scaffold could increase osteogenic activity in the presence of inflammatory cytokines. We report mechanical properties of a mineralized collagen-amnion scaffold and investigated osteogenic differentiation and mineral deposition of porcine adipose derived stem cells within these scaffolds as a function of inflammatory challenge. Incorporation of amniotic membrane matrix promotes osteogenesis similarly to un-modified mineralized collagen scaffolds, and increases in mineralized collagen-amnion scaffolds under inflammatory challenge. Together, these findings suggest that a mineralized collagen-amnion scaffold may provide a beneficial environment to aid craniomaxillofacial bone repair, especially in the course of defects presenting significant inflammatory complications.

Download Full-text

Mineralized collagen scaffolds fabricated with amniotic membrane matrix increase osteogenesis under inflammatory conditions

Regenerative Biomaterials ◽

10.1093/rb/rbaa005 ◽

2020 ◽

Vol 7 (3) ◽

pp. 247-258

Author(s):

Marley J Dewey ◽

Eileen M Johnson ◽

Simona T Slater ◽

Derek J Milner ◽

Matthew B Wheeler ◽

...

Keyword(s):

Osteogenic Differentiation ◽

Amniotic Membrane ◽

Bone Repair ◽

Composite Scaffold ◽

High Energy ◽

Collagen Scaffolds ◽

Membrane Matrix ◽

Inflammatory Conditions ◽

Mineral Deposition ◽

Mineralized Collagen

Abstract Defects in craniofacial bones occur congenitally, after high-energy impacts, and during the course of treatment for stroke and cancer. These injuries are difficult to heal due to the overwhelming size of the injury area and the inflammatory environment surrounding the injury. Significant inflammatory response after injury may greatly inhibit regenerative healing. We have developed mineralized collagen scaffolds that can induce osteogenic differentiation and matrix biosynthesis in the absence of osteogenic media or supplemental proteins. The amniotic membrane is derived from placentas and has been recently investigated as an extracellular matrix to prevent chronic inflammation. Herein, we hypothesized that a mineralized collagen–amnion composite scaffold could increase osteogenic activity in the presence of inflammatory cytokines. We report mechanical properties of a mineralized collagen–amnion scaffold and investigated osteogenic differentiation and mineral deposition of porcine adipose-derived stem cells within these scaffolds as a function of inflammatory challenge. Incorporation of amniotic membrane matrix promotes osteogenesis similarly to un-modified mineralized collagen scaffolds, and increases in mineralized collagen–amnion scaffolds under inflammatory challenge. Together, these findings suggest that a mineralized collagen–amnion scaffold may provide a beneficial environment to aid craniomaxillofacial bone repair, especially in the course of defects presenting significant inflammatory complications.

Download Full-text

Effective Keratocyte Culture Using Amniotic Membrane Matrix and Differentiation of Mesenchymal Stem Cells

Journal of the Korean Ophthalmological Society ◽

10.3341/jkos.2010.51.12.1652 ◽

2010 ◽

Vol 51 (12) ◽

pp. 1652 ◽

Cited By ~ 1

Author(s):

Soo Hyun Park ◽

Yeoun Sook Chun ◽

Jae Chan Kim

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Amniotic Membrane ◽

Membrane Matrix

Download Full-text

Biocompatible collagen scaffolds from a human amniotic membrane: physicochemical and in vitro culture characteristics

Journal of Biomaterials Science Polymer Edition ◽

10.1163/156856203322274941 ◽

2003 ◽

Vol 14 (7) ◽

pp. 689-706 ◽

Cited By ~ 25

Author(s):

T. R. Kumar ◽

N. Shanmugasundaram ◽

M. Babu

Keyword(s):

In Vitro Culture ◽

Amniotic Membrane ◽

Human Amniotic Membrane ◽

Collagen Scaffolds ◽

Culture Characteristics

Download Full-text

Effect of Amniotic Membrane/Collagen-Based Scaffolds on the Chondrogenic Differentiation of Adipose-Derived Stem Cells and Cartilage Repair

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2021.647166 ◽

2021 ◽

Vol 9 ◽

Author(s):

Le Cao ◽

Yuling Tong ◽

Xiao Wang ◽

Qiang Zhang ◽

Yiying Qi ◽

...

Keyword(s):

Articular Cartilage ◽

Cartilage Repair ◽

Amniotic Membrane ◽

Adipose Derived Stem Cells ◽

Collagen Scaffolds ◽

Biological Scaffolds ◽

Post Operation ◽

And Cartilage ◽

Membrane Collagen

Objectives: Repairing articular cartilage damage is challenging. Clinically, tissue engineering technology is used to induce stem cell differentiation and proliferation on biological scaffolds to repair defective joints. However, no ideal biological scaffolds have been identified. This study investigated the effects of amniotic membrane/collagen scaffolds on the differentiation of adipose-derived stem cells (ADSCs) and articular cartilage repair.Methods: Adipose tissue of New Zealand rabbits was excised, and ADSCs were isolated and induced for differentiation. An articular cartilage defect model was constructed to identify the effect of amniotic membrane/collagen scaffolds on cartilage repair. Cartilage formation was analyzed by imaging and toluene blue staining. Knee joint recovery in rabbits was examined using hematoxylin and eosin, toluidine, safranine, and immunohistochemistry at 12 weeks post-operation. Gene expression was examined using ELISA, RT-PCR, Western blotting, and immunofluorescence.Results: The adipose tissue was effectively differentiated into ADSCs, which further differentiated into chondrogenic, osteogenic, and lipogenic lineages after 3 weeks’ culture in vitro. Compared with platelet-rich plasmon (PRP) scaffolds, the amniotic membrane scaffolds better promoted the growth and differentiation of ADSCs. Additionally, scaffolds containing the PRP and amniotic membrane efficiently enhanced the osteogenic differentiation of ADSCs. The levels of COL1A1, COL2A1, COL10A1, SOX9, and ACAN in ADSCs + amniotic membrane + PRP group were significantly higher than the other groups both in vitro and in vivo. The Wakitani scores of the ADSC + amniotic membrane + PRP group were lower than that in ADSC + PRP (4.4 ± 0.44**), ADSC + amniotic membrane (2.63 ± 0.38**), and control groups (6.733 ± 0.21) at week 12 post-operation. Osteogenesis in rabbits of the ADSC + amniotic membrane + PRP group was significantly upregulated when compared with other groups. Amniotic membranes significantly promoted the expression of cartilage regeneration-related factors (SOX6, SOX9, RUNX2, NKX3-2, MEF2C, and GATA4). The ADSC + PRP + amniotic membrane group exhibited the highest levels of TGF-β, PDGF, and FGF while exhibiting the lowest level of IL-1β, IL6, and TNF-α in articular cavity.Conclusion: Amniotic membrane/collagen combination-based scaffolds promoted the proliferation and cartilage differentiation of ADSCs, and may provide a new treatment paradigm for patients with cartilage injury.

Download Full-text