BBS7–SHH Signaling Activity Regulates Primary Cilia for Periodontal Homeostasis

Objectives: Mechanical stimuli are essential for the maintenance of periodontal ligament (PDL) homeostasis. Although there are several studies on atrophic changes in PDL due to occlusal hypofunction, the underlying mechanism is still unknown. Here, we aimed to explore the changes of gene expression in occlusal hypofunctional PDL and elucidate the related role in maintaining the PDL homeostasis.Methods: To investigate the transcriptomic difference between control and hypofunctional PDL tissue from patients, RNA sequencing was performed on 34 human teeth. The atrophic changes in PDL were evaluated by histological analysis. The effect of the Bardet-Biedl syndrome 7 (BBS7) knockdown was evaluated by the RT-qPCR, Western blot, wound healing, and tubule formation assay.Results: We detected that the expression of BBS7 was downregulated in occlusal hypofunctional PDL through RNA sequencing. Dynamic changes, including the number of periodontal ligament cells, alignment of collagen fibers, diameter of blood vessels, appearance of primary cilia, and torturous oxytalan fibers, were observed following occlusal hypofunction. Furthermore, Sonic hedgehog signaling (Shh) activity was closely associated with BBS7 expression in PDL cells. In addition, the cell migration and angiogenesis were also suppressed by BBS7 knockdown in vitro.Conclusion: We suggest that BBS7 plays an essential role in maintaining Shh signaling activity for PDL homeostasis.

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Basic Biological Sciences Isolation and Growth of Human Periodontal Ligament Cells in vitro

Journal of Dental Research ◽

10.1177/00220345850640080101 ◽

1985 ◽

Vol 64 (8) ◽

pp. 1026-1030 ◽

Cited By ~ 82

Author(s):

B. Ragnarsson ◽

G. Carr ◽

J.C. Daniel

Keyword(s):

Biological Sciences ◽

Epithelial Cell ◽

Epithelial Cells ◽

Periodontal Ligament ◽

Root Surface ◽

Cell Protein ◽

Periodontal Ligament Cells ◽

Human Teeth ◽

Two Populations

The periodontal ligaments from single human teeth were dissected free of the root surface and plated into culture dishes. In each case two populations of cells were obtained: fibroblasts and epithelial cells. These two populations were separated and grown in culture. The epithelial nature of the epithelioid-appearing cells was confirmed by staining for keratin, exclusively an epithelial cell protein.

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Potential of the propolis as storage medium to preserve the viability of cultured human periodontal ligament cells: an in vitro study

Dental Traumatology ◽

10.1111/j.1600-9657.2011.00974.x ◽

2011 ◽

Vol 27 (2) ◽

pp. 102-108 ◽

Cited By ~ 13

Author(s):

Payal Saxena ◽

Vandana Aditya Pant ◽

Kulvindar Kaur Wadhwani ◽

Mahendra Pratap Kashyap ◽

Saurabh Kumar Gupta ◽

...

Keyword(s):

Periodontal Ligament ◽

In Vitro Study ◽

Vitro Study ◽

Periodontal Ligament Cells ◽

Storage Medium ◽

Human Periodontal Ligament Cells

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Effects of enamel matrix proteins on multi-lineage differentiation of periodontal ligament cells in vitro

Acta Biomaterialia ◽

10.1016/j.actbio.2012.09.008 ◽

2013 ◽

Vol 9 (1) ◽

pp. 4796-4805 ◽

Cited By ~ 20

Author(s):

Harsh D. Amin ◽

Irwin Olsen ◽

Jonathan C. Knowles ◽

Michel Dard ◽

Nikolaos Donos

Keyword(s):

Periodontal Ligament ◽

Matrix Proteins ◽

Periodontal Ligament Cells ◽

Enamel Matrix ◽

Enamel Matrix Proteins ◽

Lineage Differentiation

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In-vitro cytocompatibility of self-adhesive dual-curing resin cements on human mesenchymal stem cells (hMSC) and periodontal ligament cells (PDL-hTERT)

Dental Materials ◽

10.1016/j.dental.2021.12.020 ◽

2022 ◽

Author(s):

Iris Frasheri ◽

Alexandra Grimm ◽

Christina Ern ◽

Reinhard Hickel ◽

Matthias Folwaczny

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Periodontal Ligament ◽

Human Mesenchymal Stem Cells ◽

Periodontal Ligament Cells ◽

Resin Cements ◽

Dual Curing

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The effects of a combination of calcium sulfate and platelet-derived growth factor on periodontal ligament cells in vitro

The Journal of the Korean Academy of Periodontology ◽

10.5051/jkape.1997.27.4.785 ◽

1997 ◽

Vol 27 (4) ◽

pp. 785

Author(s):

Jun Seong Kim ◽

Seong Ho Choi ◽

Yun Jung Yu ◽

Jung Kiu Chai ◽

Chong Kwan Kim ◽

...

Keyword(s):

Growth Factor ◽

Periodontal Ligament ◽

Calcium Sulfate ◽

Platelet Derived Growth Factor ◽

Periodontal Ligament Cells

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Functional Behaviour of Human Periodontal Ligament Cells around Various Dental Implants

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.361-363.837 ◽

2007 ◽

Vol 361-363 ◽

pp. 837-840 ◽

Cited By ~ 3

Author(s):

Xiao Ting Luo ◽

Zhen Gao ◽

Shi Gui Yan ◽

Wei Deng ◽

Wen Shu Zhang ◽

...

Keyword(s):

Surface Roughness ◽

Dental Implants ◽

Periodontal Ligament ◽

Cell Counting ◽

Immunofluorescent Staining ◽

Periodontal Ligament Cells ◽

Human Periodontal Ligament Cells ◽

Proliferation And Differentiation ◽

In Vitro Systems

In the present investigation, four titanium (Ti) surfaces of dental implants were compared through in vitro systems. The surface roughness of Ti was measured by TR240 mobile surface roughmeter. The Ti implants were seeded with human periodontal ligament cells (hPLDCs) and maintained for a period of 0-7 days. The adhesion, proliferation, and differentiation of hPLDCs were observed by using Cell morphology, cell counting and Osteocalcin (OC) immunofluorescent staining. Results suggest that surface roughness of titanium favors hPDLCs behavior and improves cell adhesion, proliferation, and differentiation.

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