Latest Advances in Endothelial Progenitor Cell-Derived Extracellular Vesicles Translation to the Clinic

Almost all nucleated cells secrete extracellular vesicles (EVs) that are heterogeneous spheroid patterned or round shape particles ranging from 30 to 200 nm in size. Recent preclinical and clinical studies have shown that endothelial progenitor cell-derived EVs (EPC-EVs) have a beneficial therapeutic effect in various diseases, including cardiovascular diseases and kidney, and lung disorders. Moreover, some animal studies have shown that EPC-EVs selectively accumulate at the injury site with a specific mechanism of binding along with angiogenic and restorative effects that are superior to those of their ancestors. This review article highlights current advances in the biogenesis, delivery route, and long-term storage methods of EPC-EVs and their favorable effects such as anti-inflammatory, angiogenic, and tissue protection in various diseases. Finally, we review the possibility of therapeutic application of EPC-EVs in the clinic.

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Endothelial Progenitor Cell Function Inversely Correlates With Long-term Glucose Control in Diabetic Patients: Association With the Attenuation of the Heme Oxygenase-Adiponectin Axis

Canadian Journal of Cardiology ◽

10.1016/j.cjca.2012.01.013 ◽

2012 ◽

Vol 28 (6) ◽

pp. 728-736 ◽

Cited By ~ 28

Author(s):

Yossi Issan ◽

Edith Hochhauser ◽

Ran Kornowski ◽

Dorit Leshem-Lev ◽

Eli Lev ◽

...

Keyword(s):

Heme Oxygenase ◽

Endothelial Progenitor Cell ◽

Glucose Control ◽

Progenitor Cell ◽

Cell Function ◽

Diabetic Patients ◽

Endothelial Progenitor

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Prolonged Fasting Improves Endothelial Progenitor Cell-Mediated Ischemic Angiogenesis in Mice

Cellular Physiology and Biochemistry ◽

10.1159/000452581 ◽

2016 ◽

Vol 40 (3-4) ◽

pp. 693-706 ◽

Cited By ~ 6

Author(s):

Bao Xin ◽

Chun-Long Liu ◽

Hong Yang ◽

Cheng Peng ◽

Xiao-Hui Dong ◽

...

Keyword(s):

Cerebral Ischemia ◽

Endothelial Progenitor Cell ◽

Progenitor Cell ◽

Ischemic Injury ◽

Prolonged Fasting ◽

Endothelial Progenitor ◽

Ischemic Brain ◽

Cerebral Ischemic Injury ◽

Cerebral Ischemic

Background/Aims: Prolonged fasting (PF) was shown to be of great potency to promote optimal health and reduce the risk of many chronic diseases. This study sought to determine the effect of PF on the endothelial progenitor cell (EPC)-mediated angiogenesis in the ischemic brain and cerebral ischemic injury in mice. Methods: Mice were subjected to PF or periodic PF after cerebral ischemia, and histological analysis and behavioral tests were performed. Mouse EPCs were isolated and examined, and the effects of EPC transplantation on cerebral ischemic injury were investigated in mice. Results: It was found that PF significantly increased the EPC functions and angiogenesis in the ischemic brain, and attenuated the cerebral ischemic injury in mice that was previously subjected to cerebral ischemia. Periodic PF might reduce cortical atrophy and improve long-term neurobehavioral outcomes after cerebral ischemia in mice. The eNOS and MnSOD expression and intracellular NO level were increased, and TSP-2 expression and intracellular O2- level were reduced in EPCs from PF-treated mice compared to control. In addition, transplanted EPCs might home into ischemic brain, and the EPCs from PF-treated mice had a stronger ability to promote angiogenesis in ischemic brain and reduce cerebral ischemic injury compared to the EPCs from control mice. The EPC-conditioned media from PF-treated mice exerted a stronger effect on cerebral ischemic injury reduction compared to that from control mice. Conclusion: Prolonged fasting promoted EPC-mediated ischemic angiogenesis and improved long-term stroke outcomes in mice. It is implied that prolonged fasting might potentially be an option to treat ischemic vascular diseases.

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