scholarly journals Profiling Antibody Response Patterns in COVID-19: Spike S1-Reactive IgA Signature in the Evolution of SARS-CoV-2 Infection

2021 ◽  
Vol 12 ◽  
Author(s):  
Gabriel Siracusano ◽  
Chiara Brombin ◽  
Claudia Pastori ◽  
Federica Cugnata ◽  
Maddalena Noviello ◽  
...  
Keyword(s):  
Antibody Response ◽  
Clinical Manifestations ◽  
Surrogate Marker ◽  
Response Patterns ◽  
Specific Antibody Response ◽  
Virus Specific Antibody ◽  
Key Drivers ◽  
Severity Of The Disease ◽  
Disease Antibody ◽  
Antibody Levels

This contribution explores in a new statistical perspective the antibody responses to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in 141 coronavirus disease 2019 (COVID-19) patients exhibiting a broad range of clinical manifestations. This cohort accurately reflects the characteristics of the first wave of the SARS-CoV-2 pandemic in Italy. We determined the IgM, IgA, and IgG levels towards SARS-CoV-2 S1, S2, and NP antigens, evaluating their neutralizing activity and relationship with clinical signatures. Moreover, we longitudinally followed 72 patients up to 9 months postsymptoms onset to study the persistence of the levels of antibodies. Our results showed that the majority of COVID-19 patients developed an early virus-specific antibody response. The magnitude and the neutralizing properties of the response were heterogeneous regardless of the severity of the disease. Antibody levels dropped over time, even though spike reactive IgG and IgA were still detectable up to 9 months. Early baseline antibody levels were key drivers of the subsequent antibody production and the long-lasting protection against SARS-CoV-2. Importantly, we identified anti-S1 IgA as a good surrogate marker to predict the clinical course of COVID-19. Characterizing the antibody response after SARS-CoV-2 infection is relevant for the early clinical management of patients as soon as they are diagnosed and for implementing the current vaccination strategies.

Parenterally transmitted non-A, non-B hepatitis: Virus-specific antibody response patterns in hepatitis C virus-infected chimpanzees

1990 ◽  
Vol 99 (4) ◽  
pp. 1054-1060 ◽  
Author(s):  
Daniel W. Bradley ◽  
Krzysztof Krawczynski ◽  
James W. Ebert ◽  
Karen A. McCaustland ◽  
Qui-Lim Choo ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Antibody Response ◽  
Specific Antibody ◽  
Hepatitis Virus ◽  
Response Patterns ◽  
Specific Antibody Response ◽  
Virus Specific Antibody

scholarly journals Evaluation of antibody response to Goat Pox cell culture vaccine in goats in India

2017 ◽  
Vol 33 (1) ◽  
pp. 23-27
Author(s):  
S Baksi ◽  
P Puwar ◽  
N Rao ◽  
R Oza
Keyword(s):  
Antibody Response ◽  
Total Duration ◽  
Small Ruminants ◽  
Economic Loss ◽  
Viral Disease ◽  
High Morbidity ◽  
Specific Antibody Response ◽  
Economic Significance ◽  
Neutralization Index ◽  
Antibody Levels

Goat Pox is an important contagious viral disease that causes serious economic loss in small ruminants. The disease causes high morbidity, mortality and trade restriction. Several outbreaks of the disease are reported in Africa and Europe. In India also, the disease has high economic significance. Present study was undertaken to estimate specific antibody response to Live Goat Pox Vaccine in goats. Thirty-five healthy goats were vaccinated with single dose and 10 goats were kept as control. Blood was collected at defined intervals and sera were separated. Virus neutralization test was performed to estimate neutralization index as marker of antibody levels in serum. The study revealed that vaccine could induce immunity against the disease within seven days of vaccination, and reached a peak at 21 days. The titre remained at protective level throughout the oneyear study. Further study can be done to estimate the total duration of immunity through extended study, and the response to challenge with virus.Bangl. vet. 2016. Vol. 33, No. 1, 23-27

scholarly journals Comparative evaluation of SARS-CoV-2 IgG assays against nucleocapsid and spike antigens

2021 ◽  
pp. 1-5
Author(s):  
Mitra Rezaei ◽  
Mohammadhadi Sadeghi ◽  
Alireza Korourian ◽  
Payam Tabarsi ◽  
Mihan Porabdollah ◽  
...  
Keyword(s):  
Antibody Response ◽  
Symptom Onset ◽  
Disease Onset ◽  
Early Time ◽  
Igg Antibody ◽  
Cell Percentage ◽  
Percent Positivity ◽  
Severity Of The Disease ◽  
Nucleoprotein N ◽  
Antibody Levels

BACKGROUND: There are few studies to compare antibody response against anti-spike (S) and anti- nucleoprotein (N) SARS-CoV-2. OBJECTIVE: The aim of this study was to evaluate the IgG antibody production against S and N antigens of the virus and their correlation with the time and severity of the disease. METHODS: The IgG antibodies against S and N antigens of SARS-CoV-2 in serum specimens 72 symptomatic patients who tested real-time reverse transcription polymerase chain reaction positive for SARS-CoV-2 were detected using the ELISA technique. Different antibody response was compared and the correlation with the time from disease onset and the severity was evaluated. RESULTS: Forty-eight of 72 (67%) patients tested positive for anti-SARS-CoV-2 antibodies, while 24 (33%) did not have detectable antibodies. Comparison of antibody levels for N and S antibodies showed that they correlate with each other well (r= 0.81; P< 0.001). However, sensitivity of anti-S SARS-CoV-2 IgG and anti-N SARS-CoV-2 IgG was 30% and 60%, during the first 7 days after symptom onset (r= 0.53; P= 0.111), but increased to 73% and 68% at more than 1-week post symptom onset (r= 0.89, P= 0.111), respectively. Cases with positive IgG response showed a decreased CD8 cell percentage compared to the negative IgG groups (26 ± 14 vs. 58 ± 32, p= 0.066 in anti-N IgG group and 28 ± 15 vs. 60 ± 45, p= 0.004 in anti-S IgG group, respectively). CONCLUSION: Nearly one-third of the confirmed COVID-19 patients had negative serology results. Lower percent positivity at early time points after symptom onset (less than 1 week) was seen using anti-S SARS-COV-2 IgG kit compare to the anti-N SARS-CoV-2 IgG; therefore, clinicians should interpret negative serology results of especially anti-S SARS-CoV-2 IgG with caution.

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