scholarly journals A Dual-Factor Releasing Hydrogel for Rotator Cuff Injury Repair

2021 ◽  
Vol 8 ◽  
Author(s):  
Chong Teng ◽  
Yifei Fang ◽  
Huangrong Zhu ◽  
Leyi Huang ◽  
Yanglei Jin ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Surgical Reconstruction ◽  
Repair Capacity ◽  
Bone Interface ◽  
Injury Repair ◽  
Injured Area ◽  
Rotator Cuff Injury ◽  
Two Factors ◽  
Fast Healing

Rotator cuff injury causes pain in the shoulder and is a challenge to be repaired even after surgical reconstruction. Here, we developed a dual-factor releasing hydrogel based on sulfhydrylated chitosan to deliver KGN and FGF-2 to the injured area to enable fast healing of the tendon–bone interface, which is essential for the repair of rotator cuff injury. We found that the two factors could be easily loaded into the hydrogel, which could in turn continuously release the factors in physiological conditions. The hydrogel was found to be a porous structure through a scanning electron microscope (SEM). The micropores in the hydrogel structure enable the loading and releasing of these molecules. This study showed that KGN and FGF-2 could play a synergistic effect by recruiting and promoting stem cell proliferation and chondrogenesis, thus accelerating the healing of the tendon–bone interface. An in vivo study based on a rabbit rotator cuff injury model demonstrated that the dual-factor releasing hydrogel possesses superior repair capacity than a single-factor releasing hydrogel and the untreated groups. In conclusion, the KGN and FGF-2 dual-factor releasing hydrogel could be a promising biomaterial for the regeneration of the tendon–bone interface and rotator cuff injury repair.

scholarly journals Fibrin Glue-Kartogenin Complex Promotes the Regeneration of the Tendon-Bone Interface in Rotator Cuff Injury

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Jun Zhu ◽  
Jiahua Shao ◽  
Yi Chen ◽  
Guangyi Zhao ◽  
Lexiang Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Rotator Cuff ◽  
Fibrin Glue ◽  
Tendon Graft ◽  
Bone Tunnel ◽  
Rotator Cuff Tendon ◽  
Bone Interface ◽  
Tunnel Model ◽  
Rotator Cuff Injury

Objective. Rotator cuff injury healing is problematic because the tendon-bone junction often forms cicatricial tissues, rather than fibrocartilage, which leads to mechanical impairment and is prone to redamage. Kartogenin (KGN) is a newly discovered small molecule compound which can induce cartilage formation through chondrogenesis of endogenous mesenchymal stem cells. Methods. In this study, we used KGN with fibrin glue (FG) to repair the rotator cuff injury by promoting the formation of fibrocartilage at the tendon to bone interface. Firstly, we assessed the release rate of KGN from the FG-KGN complex and then created a rabbit rotator cuff tendon graft-bone tunnel model. The rabbits received saline, FG-KGN, or FG injections onto the tendon to bone interface after injury. Shoulder tissues were harvested at 6 and 12 weeks, and the sections were stained with HE and Safranin O/Fast green. The samples were assessed by histologic evaluation and biomechanical testing. Synovial mesenchymal stem cells derived from the synovial tissue around the rotator cuff were harvested for western blotting and qRT-PCR analysis. Results. KGN was released rapidly from the FG-KGN complex during first 4 hrs and followed by a slow release until 7 days. The tendon graft-bone interface in the control (saline) group and the FG group was filled with scar tissue, rather than cartilage-like tissue, and only a small number of chondrocytes were found at the adjacent bone surface. In the FG-KGN group, the tendon to bone interface was fully integrated and populated by chondrocytes with proteoglycan deposition, indicating the formation of fibrocartilage-like tissues. At 12 weeks, the maximum tensile strength of the FG-KGN group was significantly higher than that of the FG and control groups ( P < 0.01 ). The RNA expression levels of tendinous genes such as Tenascin C and the chondrogenic gene Sox-9 were substantially elevated in SMSCs treated with the FG-KGN complex compared to the other two groups. Conclusion. These results indicated that fibrin glue is an effective carrier for KGN, allowing for the sustained release of KGN. The FG-KGN complex could effectively promote the regeneration and formation of fibrocartilage tissue of the tendon-bone interface in the rabbit rotator cuff tendon graft-bone tunnel model.

Biomimetic Nanofiber Scaffolds for Tendon-to-Bone Insertion Repair

2014 ◽  
Vol 875-877 ◽  
pp. 331-334 ◽  
Author(s):  
Jing Wei Xie ◽  
Bing Ma ◽  
Franklin D. Shuler
Keyword(s):  
Rotator Cuff ◽  
Fiber Orientation ◽  
Mineral Content ◽  
Collagen Fiber ◽  
Insertion Site ◽  
Injury Repair ◽  
Nanofiber Scaffolds ◽  
Rotator Cuff Injury ◽  
Fiber Organization

This paper reports the fabrication of nanofiber scaffolds with dual gradients in both mineral content and fiber orientation, which can be used to mimic the composition and collagen fiber organization at native tendon-to-bone insertion site. Such scaffolds show great potential for rotator cuff injury repair.

Human Tendon–Derived Collagen Hydrogel Significantly Improves Biomechanical Properties of the Tendon-Bone Interface in a Chronic Rotator Cuff Injury Model

2019 ◽  
Vol 44 (10) ◽  
pp. 899.e1-899.e11 ◽  
Author(s):  
Yukitoshi Kaizawa ◽  
Jacinta Leyden ◽  
Anthony W. Behn ◽  
U. Serdar Tulu ◽  
Austin Franklin ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Biomechanical Properties ◽  
Bone Interface ◽  
Collagen Hydrogel ◽  
Injury Model ◽  
Rotator Cuff Injury ◽  
Human Tendon

scholarly journals Hu Qian Wan Decoction Promotes Tendon-bone Healing of the Rotator Cuff in Rats by Inducing the Release of Exosomes Containing Interleukin-1 Receptor Antagonists

2020 ◽  
Author(s):  
Yao Huang ◽  
Lei Wang ◽  
Bing He ◽  
Fu-cheng Zhang ◽  
Bin Yuan ◽  
...  
Keyword(s):  
Rotator Cuff ◽  
Bone Healing ◽  
Interleukin 1 ◽  
Breaking Load ◽  
Receptor Protein ◽  
Intragastric Administration ◽  
Bone Interface ◽  
Macrophage Cells

Abstract Background: Hu Qian Wan decoction (HQWD) is a traditional Chinese medicine used to treat many orthopedic diseases, including osteoporosis and osteoarthritis. This study aims to explore the mechanism by which HQWD promotes tendon-bone healing in the rotator cuff.Methods: Tendon-bone healing of the rotator cuff, serum exosome expression, and changes in interleukin-1 receptor antagonist (IL-1RA) in exosomes were analyzed after intragastric administration of HQWD in the rotator cuff reconstruction model of rats. The effects of Hu Qian Wan (HQW) on exosomes, IL-1RA, and Nod-like receptor protein 3 (NLRP3) secretion were verified in rat bone marrow mesenchymal stem cells (BMSCs) and macrophage cells, and the effects of HQW on inflammasomes were observed. Results: HQWD intragastric administration increased proteoglycan and collagen Ⅰ (Col Ⅰ) expression in the tendon-bone interface, improved the tendon-bone interface area, and augmented the maximum breaking load and stiffness of the rotator cuff. HQWD intragastric administration also increased rat exosome and IL-1RA expression in vivo. HQW promoted exosome and IL-1RA expression and suppressed the activation of NLRP3 inflammasomes in rat BMSCs and macrophage cells in vitro. Conclusions: HQWD inhibited inflammasome-related inflammation by inducing exosome and IL-1RA secretion in rats, thus, promoting tendon-bone healing of the rotator cuff.

Application of Calcium Phosphate Biological Material on Rehabilitation in Exercise-Induced Rotator Cuff Injury

2014 ◽  
Vol 707 ◽  
pp. 184-187
Author(s):  
Chao Zhao ◽  
Zhen Lei Ma ◽  
Lei Zhang
Keyword(s):  
Calcium Phosphate ◽  
Rotator Cuff ◽  
Biomechanical Properties ◽  
Repair Process ◽  
Early Recovery ◽  
Rotator Cuff Tendon ◽  
Bone Interface ◽  
Rotator Cuff Injury ◽  
Functional Exercise ◽  
Exercise Induced

Rotator cuff injury is one of the most common musculoskeletal system diseases in exercise-induced injury, self-setting calcium phosphate materials has a very good promoting role in the rotator cuff tendon bone interface in the repair process, it can effectively improve the maximum tensile strength of tendon bone interface and stiffness, and promote recovery of biomechanical properties. Therefore, the use of calcium phosphate artificial material can promote repair of the tendon bone interface in exercise-induced rotator cuff injury, and achieve early functional exercise, and early recovery of limb function and ability to live and work.

Surface modification of the simvastatin factor-loaded silk fibroin promotes the healing of rotator cuff injury through β-catenin signaling

2021 ◽  
pp. 088532822199592
Author(s):  
Liang Hao ◽  
Jun Chen ◽  
Xiliang Shang ◽  
Shiyi Chen
Keyword(s):  
Surface Modification ◽  
Rotator Cuff ◽  
Osteogenic Differentiation ◽  
Silk Fibroin ◽  
In Vitro Experiments ◽  
Sd Rats ◽  
Rotator Cuff Injuries ◽  
Rotator Cuff Injury

Rupture of the rotator cuff is a common injury of the shoulder joint in sports professionals. In addition, research on repair of the rotator cuff has gained popularity over the recent years. Given the high rate of re-tear after surgery, it is necessary to design and prepare biodegradable materials with good mechanical properties, for the management of the condition. Consequently, the present study conducted surface modification of the simvastatin factor-loaded silk fibroin for the repair of chronic rotator cuff injury in SD rats. The in vitro experiments were analyzed through scanning electron microscopy and the water contact angle. Additionally, the CCK-8 assay was used to observe the effect of the intervention on the proliferation of BMSCs. Moreover, the osteogenic differentiation of BMSCs was detected through the ALP and ARS assays while the expression of osteogenic genes was examined using qRT-PCR and Western blot analysis. Furthermore, a model for repairing chronic rotator cuff tears in SD rats was established in vivo. Thereafter, rotator cuff repair and healing were evaluated through HE staining while Masson and Sirius staining was used to detect the collagen formation ratio. Additionally, the study analyzed the mechanism underlying the effect of simvastatin-loaded silk fibroin. The results showed that the simvastatin-loaded silk fibroin membrane had better biocompatibility and the in vitro experiments confirmed that it could promote the proliferation and osteogenic differentiation of BMSCs. In addition, the in vivo HE staining experiments similarly confirmed that it could enhance tendon bone healing and alleviate inflammation in chronic rotator cuff injuries. On the other hand, Masson and Sirius staining showed that the simvastatin-loaded silk fibroin could promote the formation of collagen. Further analysis also revealed that it could promote the osteogenic differentiation of BMSCs by activating the β-catenin signaling pathway. In general, these findings suggested that surface modification of the simvastatin factor-loaded silk fibroin was a potential means of improving the healing of rotator cuff injuries and can be implemented in clinical practice in future.

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