Cooperative pathogenicity in cystic fibrosis: Stenotrophomonas maltophilia modulates Pseudomonas aeruginosa virulence in mixed biofilm

Background: The epidemiology of cystic fibrosis (CF) associated pathogens other than Pseudomonas aeruginosa in the South African cystic fibrosis population has not been previously described.Methods: A retrospective review of respiratory cultures taken from cystic fibrosis clinic patients at the Charlotte Maxeke Johannesburg Academic Hospital from 2006 to 2010 was performed.Results: During the study period, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Burkholderia cepacia complex and Candida albicans prevalence remained stable, Aspergillus fumigatus increased from 8% to 20% (p = 0.0132); Staphylococcus aureus decreased from 66% to 50% (p = 0.0243) and Haemophilus influenzae decreased from 13% to 3% (p = 0.0136). There were significant antimicrobial susceptibility changes to meropenem (p 0.0001) amongst P. aeruginosa isolates and cloxacillin (p 0.0001) amongst S. aureus isolates. Prevalence of most bacterial pathogens appeared to increase with increasing age.Conclusion: The findings of this study illustrate the epidemiology of CF associated respiratory pathogens and the trends in prevalence and susceptibility patterns over a 5-year period.

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Effectors of the Stenotrophomonas maltophilia Type IV Secretion System Mediate Killing of Clinical Isolates of Pseudomonas aeruginosa

mBio ◽

10.1128/mbio.01502-21 ◽

2021 ◽

Author(s):

Megan Y. Nas ◽

Jeffrey Gabell ◽

Nicholas P. Cianciotto

Keyword(s):

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

Stenotrophomonas Maltophilia ◽

Opportunistic Pathogen ◽

Lung Infection ◽

Secretion System ◽

Type Iv Secretion ◽

Type Iv Secretion System ◽

Content Type ◽

Type Iv

S. maltophilia is an increasingly important opportunistic pathogen. Inherently resistant to many antibiotics, S. maltophilia is often associated with lung infection, being, among other things, a complicating factor in cystic fibrosis patients.

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Comparison of in vitro susceptibilities to levofloxacin and ciprofloxacin with Pseudomonas aeruginosa and Stenotrophomonas maltophilia isolated from cystic fibrosis patients in Northern Ireland

British Journal of Biomedical Science ◽

10.1080/09674845.2005.11978067 ◽

2005 ◽

Vol 62 (1) ◽

pp. 30-32 ◽

Cited By ~ 2

Author(s):

A-J. Mcknight ◽

A. Shaw ◽

C.E. Goldsmith ◽

L. Clarke ◽

B.C. Millar ◽

...

Keyword(s):

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

Northern Ireland ◽

Stenotrophomonas Maltophilia

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In Vitro Activity of Ceftolozane-Tazobactam against Multidrug-Resistant Nonfermenting Gram-Negative Bacilli Isolated from Patients with Cystic Fibrosis

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02688-16 ◽

2017 ◽

Vol 61 (4) ◽

Cited By ~ 11

Author(s):

Patrick Grohs ◽

Gary Taieb ◽

Philippe Morand ◽

Iheb Kaibi ◽

Isabelle Podglajen ◽

...

Keyword(s):

Cystic Fibrosis ◽

Pseudomonas Aeruginosa ◽

Stenotrophomonas Maltophilia ◽

Active Agent ◽

Multidrug Resistant ◽

In Vitro Activity ◽

Gram Negative ◽

Content Type ◽

Time Kill

ABSTRACT Ceftolozane-tazobactam was tested against 58 multidrug-resistant nonfermenting Gram-negative bacilli (35 Pseudomonas aeruginosa, 11 Achromobacter xylosoxydans, and 12 Stenotrophomonas maltophilia isolates) isolated from cystic fibrosis patients and was compared to ceftolozane alone, ceftazidime, meropenem, and piperacillin-tazobactam. Ceftolozane-tazobactam was the most active agent against P. aeruginosa but was inactive against A. xylosoxydans and S. maltophilia. In time-kill experiments, ceftolozane-tazobactam had complete bactericidal activity against 2/6 clinical isolates (33%).

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